Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,380,447 C→T 7.0% P86L (CCG→CTG)  yhcB → DUF1043 family inner membrane‑anchored protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,380,4470CT 7.0% 372.2 / 13.4 116P86L (CCG→CTG) yhcBDUF1043 family inner membrane‑anchored protein
Reads supporting (aligned to +/- strand):  ref base C (52/54);  new base T (4/4);  total (57/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 120 total aligned reads displayed.

AAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGA  >  NC_000913/3380308‑3380578
                                                                                                                                           |                                                                                                                                   
aaGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCGGCAGCAGGCTGCTgg                                                                                                                                   >  1:9240/1‑142 (MQ=255)
aaGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGt                                                                                                                             >  2:678846/1‑148 (MQ=255)
aaGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTCGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCGGCAGCAGGCTGCTgg                                                                                                                                   <  2:9240/142‑1 (MQ=255)
     tAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCGGCAGCAGGCTGCTgg                                                                                                                                   >  4:61947/1‑137 (MQ=255)
     tAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCGGCAGCAGGCTGCTgg                                                                                                                                   <  3:61947/137‑1 (MQ=255)
       aaGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGa                                                                                                                      <  3:142786/148‑1 (MQ=255)
             aaCTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCaaa                                                                                                                >  4:1065348/1‑148 (MQ=255)
                  gACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGt                                                                                                           <  1:678846/148‑1 (MQ=255)
                   aCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGt                                                                                                                             >  1:511071/1‑129 (MQ=255)
                        tATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTa                                                                                                     <  1:808270/148‑1 (MQ=255)
                           cgcgAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCaaa                                                                                                                <  4:1003406/134‑1 (MQ=255)
                           cgcgAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCaaa                                                                                                                <  2:725690/134‑1 (MQ=255)
                                agagCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGa                                                                                                                      >  2:907544/1‑123 (MQ=255)
                                agagCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGa                                                                                                                      <  1:907544/123‑1 (MQ=255)
                                  agCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGc                                                                                           >  3:802322/1‑148 (MQ=255)
                                        ttAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCg                                                                                                                                    >  2:805214/1‑101 (MQ=255)
                                            ccACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGaa                                                                                                                                 >  1:657856/1‑100 (MQ=255)
                                            ccACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGaa                                                                                                                                 <  2:657856/100‑1 (MQ=255)
                                              aCTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGa                                                                                                                                  <  2:292773/97‑1 (MQ=255)
                                              aCTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGa                                                                                                                                  >  1:292773/1‑97 (MQ=255)
                                               cTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTct                                                                                                                           <  3:501075/103‑1 (MQ=255)
                                               cTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTct                                                                                                                           >  4:501075/1‑103 (MQ=255)
                                               cTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGtt                                                                                                          >  4:1060279/1‑120 (MQ=255)
                                               cTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGtt                                                                                                          <  3:1060279/120‑1 (MQ=255)
                                                      cGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTa                                                                                                     >  2:74747/1‑118 (MQ=255)
                                                      cGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTa                                                                                                     <  1:74747/118‑1 (MQ=255)
                                                      cGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACg                                                                       >  3:22315/1‑148 (MQ=255)
                                                      cGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACg                                                                       <  4:22315/148‑1 (MQ=255)
                                                       gCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCa                                                                             >  3:1135252/1‑141 (MQ=255)
                                                       gCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCa                                                                             >  3:226278/1‑141 (MQ=255)
                                                       gCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCa                                                                             <  4:1135252/141‑1 (MQ=255)
                                                        cAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCGGCAGCAGGCTGCTGGAGCtt                                                                                                                              <  2:1093391/91‑2 (MQ=38)
                                                        cAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCGGCAGCAGGCTGCTGGAGCtt                                                                                                                              >  1:1093391/1‑90 (MQ=38)
                                                             cgGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGc                                                                >  4:1023235/1‑148 (MQ=255)
                                                                      tGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGa                                                                      >  1:281203/1‑133 (MQ=255)
                                                                      tGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCa                                                                   <  1:633300/136‑1 (MQ=255)
                                                                      tGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCa                                                                   >  2:633300/1‑136 (MQ=255)
                                                                          tACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGaa                                                                                                                     <  2:189083/82‑1 (MQ=255)
                                                                          tACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGaa                                                                                                                     >  1:189083/1‑82 (MQ=255)
                                                                          atctaTGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATg                                                   <  3:502443/144‑1 (MQ=255)
                                                                              aTGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGa                                                                                  <  2:279895/113‑1 (MQ=255)
                                                                              aTGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCg                                                            <  1:1073576/135‑1 (MQ=255)
                                                                              aTGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCg                                                            >  2:1073576/1‑135 (MQ=255)
                                                                              aTGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTc                                               >  4:286056/1‑148 (MQ=255)
                                                                               tGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTcg                                              <  3:286056/148‑1 (MQ=255)
                                                                                 gcgcACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCa                                                                   >  3:1034762/1‑125 (MQ=255)
                                                                                       gACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTAtt                                      <  4:802322/148‑1 (MQ=255)
                                                                                       gACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTAtt                                      <  3:1023235/148‑1 (MQ=255)
                                                                                        aCTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGc                                                                               <  2:586736/106‑1 (MQ=255)
                                                                                        aCTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGc                                                                               >  1:586736/1‑106 (MQ=255)
                                                                                          tATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGa                                                     <  2:289553/130‑1 (MQ=255)
                                                                                          tATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGa                                                     >  1:289553/1‑130 (MQ=255)
                                                                                           aTCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAg                                                      <  4:597484/128‑1 (MQ=255)
                                                                                           aTCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAg                                                      >  3:597484/1‑128 (MQ=255)
                                                                                           aTCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGa                                  <  4:277753/148‑1 (MQ=255)
                                                                                            tCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGaa                                 <  1:106461/148‑1 (MQ=255)
                                                                                               ccAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGgc                              <  1:625503/148‑1 (MQ=255)
                                                                                                cAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGAt                                                                     >  2:762709/1‑108 (MQ=255)
                                                                                                cAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGAt                                                                     <  1:762709/108‑1 (MQ=255)
                                                                                                cAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGAt                                                                     <  1:800240/108‑1 (MQ=255)
                                                                                                cAGCTCTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGAt                                                                     >  2:800240/1‑108 (MQ=255)
                                                                                                       aTCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTct                                                                                                                           <  3:1027244/47‑1 (MQ=255)
                                                                                                       aTCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTct                                                                                                                           >  4:1027244/1‑47 (MQ=255)
                                                                                                         cAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCGGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGc                                                                               <  1:1109389/89‑1 (MQ=255)
                                                                                                         cAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCGGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGc                                                                               >  2:1109389/1‑89 (MQ=255)
                                                                                                         cAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTAtt                                      >  2:306397/1‑130 (MQ=255)
                                                                                                         cAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTAtt                                      <  1:306397/130‑1 (MQ=255)
                                                                                                            cacaTGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGt                                                                                      <  2:592866/79‑1 (MQ=255)
                                                                                                            cacaTGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCa                                                                   >  2:538996/1‑98 (MQ=255)
                                                                                                            cacaTGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCa                                                                   <  1:538996/98‑1 (MQ=255)
                                                                                                                tGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGa                                                                                  <  3:1016620/79‑1 (MQ=255)
                                                                                                                tGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGa                                                                                  >  4:1016620/1‑79 (MQ=255)
                                                                                                                  gCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAAt                                                                                                   >  3:1002349/1‑60 (MQ=255)
                                                                                                                  gCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAAt                                                                                                   <  4:1002349/60‑1 (MQ=255)
                                                                                                                    aaaaaGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGa                                                                                  <  2:868083/75‑1 (MQ=255)
                                                                                                                    aaaaaGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGa                                                                                  >  1:868083/1‑75 (MQ=255)
                                                                                                                     aaaaGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGcc                                                                              >  1:7306/1‑78 (MQ=255)
                                                                                                                     aaaaGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCctg                  <  1:551659/138‑1 (MQ=255)
                                                                                                                     aaaaGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCctg                  >  2:551659/1‑138 (MQ=255)
                                                                                                                          cTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGa                                                     >  3:42275/1‑98 (MQ=255)
                                                                                                                          cTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGa                                                     <  4:42275/98‑1 (MQ=255)
                                                                                                                           tCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGa                                                     <  4:1085972/97‑1 (MQ=255)
                                                                                                                           tCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGa                                                     >  3:1085972/1‑97 (MQ=255)
                                                                                                                             cagcagCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAg                                <  3:469219/116‑1 (MQ=255)
                                                                                                                             cagcagCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAg                                >  4:469219/1‑116 (MQ=255)
                                                                                                                              agcagcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTcgcg                                            <  2:370317/103‑1 (MQ=255)
                                                                                                                              agcagcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTcgcg                                            >  1:531739/1‑103 (MQ=255)
                                                                                                                              agcagcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTcgcg                                            <  2:531739/103‑1 (MQ=255)
                                                                                                                              agcagcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCt                                    >  1:942190/1‑111 (MQ=255)
                                                                                                                              agcagcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCt                                    <  2:942190/111‑1 (MQ=255)
                                                                                                                              agcagcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGa  >  2:345440/1‑145 (MQ=255)
                                                                                                                              agcagcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGa  <  1:345440/145‑1 (MQ=255)
                                                                                                                               gcagcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAgg                               <  2:706970/115‑1 (MQ=255)
                                                                                                                               gcagcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAgg                               >  1:706970/1‑115 (MQ=255)
                                                                                                                                  gcaggctgctGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAg                                <  3:777294/107‑1 (MQ=255)
                                                                                                                                  gcaggctgctGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAg                                >  4:777294/5‑111 (MQ=255)
                                                                                                                                  gcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGa                                  >  1:798160/1‑109 (MQ=255)
                                                                                                                                  gcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGa                                  <  2:798160/109‑1 (MQ=255)
                                                                                                                                           cGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCag                                                                                         >  4:799269/1‑45 (MQ=255)
                                                                                                                                           cGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCag                                                                                         <  3:799269/45‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                   
AAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGA  >  NC_000913/3380308‑3380578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: