Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,418,845 A→C 11.8% intergenic (+234/‑197) yhdV → / → yhdW putative outer membrane protein/pseudogene, ABC transporter periplasmic binding protein family;putative transport; Not classified; putative periplasmic binding transport protein; periplasmic‑binding component of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,418,8450AC11.8% 106.2 / 7.1 34intergenic (+234/‑197)yhdV/yhdWputative outer membrane protein/pseudogene, ABC transporter periplasmic binding protein family;putative transport; Not classified; putative periplasmic binding transport protein; periplasmic‑binding component of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base A (15/15);  new base C (2/2);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GCTTTCGAAATAACGCATCAATTTAATTTTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCT  >  NC_000913/3418737‑3418946
                                                                                                            |                                                                                                     
gctTTCGAAATAACGCATCAATTTAATTTTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTc                                                                                      <  1:281287/126‑1 (MQ=255)
gctTTCGAAATAACGCATCAATTTAATTTTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTc                                                                                      >  2:281287/1‑126 (MQ=255)
gctTTCGAAATAACGCATCAATTTAATTTTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGCACCATTGCGGGgcg                                                                >  4:238208/1‑148 (MQ=255)
                 tCAATTTAATTTTCACACTTGCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAAtgtg                                                                                                 <  4:821848/98‑1 (MQ=255)
                 tCAATTTAATTTTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAAtgtg                                                                                                 >  3:821848/1‑98 (MQ=255)
                    atttaatttTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGt                                            <  3:238208/148‑1 (MQ=255)
                           tttCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAAtgt                                                                                                  <  2:396193/87‑1 (MQ=255)
                           tttCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAAtgt                                                                                                  >  1:396193/1‑87 (MQ=255)
                            ttCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGCACCATTGCGGGgc                                                                 >  1:56454/1‑119 (MQ=255)
                            ttCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGCACCATTGCGGGgc                                                                 <  2:56454/119‑1 (MQ=255)
                                     cccACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTgg                                                                                                <  4:981265/79‑1 (MQ=255)
                                     cccACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTgg                                                                                                >  3:981265/1‑79 (MQ=255)
                                        accaGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTtta                                                       >  3:1115779/1‑117 (MQ=255)
                                        accaGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTtta                                                       <  4:1115779/117‑1 (MQ=255)
                                          ccgAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGttt                                                                                       <  4:191683/81‑1 (MQ=255)
                                          caGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGttt                                                                                       >  3:191683/1‑83 (MQ=255)
                                           aGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGtt                                                           <  2:261486/110‑1 (MQ=255)
                                           aGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGtt                                                           >  1:261486/1‑110 (MQ=255)
                                             aaaTATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAAtg                                                                                                   <  4:609465/68‑1 (MQ=255)
                                             aaaTATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAAtg                                                                                                   >  3:609465/1‑68 (MQ=255)
                                               atatACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCAtttt                                                                                 >  2:857732/1‑84 (MQ=255)
                                               atatACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCAtttt                                                                                 <  1:857732/84‑1 (MQ=255)
                                                      cACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGc                                                                              >  3:531709/1‑80 (MQ=255)
                                                      cACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGc                                                                              <  4:531709/80‑1 (MQ=255)
                                                        cGCTAACAATCATTATTCTGCTTAGTAGAACACGGGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGttttt                                                        <  4:988744/100‑1 (MQ=255)
                                                        cGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGttttt                                                        >  3:988744/1‑100 (MQ=255)
                                                             aCAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGttttt                                                        >  2:678397/1‑95 (MQ=255)
                                                             aCAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGttttt                                                        <  1:678397/95‑1 (MQ=255)
                                                                                          cgTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGttt                                                          <  3:648781/64‑1 (MQ=255)
                                                                                          cgTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGttt                                                          >  4:648781/1‑64 (MQ=255)
                                                                                                    atGCGCCACATTTTGGCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCGAAAGCt  >  3:786892/2‑110 (MQ=255)
                                                                                                    atGCGCCACATTTTGGCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCGAAAGCt  <  4:786892/109‑1 (MQ=255)
                                                                                                     tGCGCCACATTTTGGCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCtt                               <  4:163828/80‑1 (MQ=255)
                                                                                                     tGCGCCACATTTTGGCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCtt                               >  3:163828/1‑80 (MQ=255)
                                                                                                            |                                                                                                     
GCTTTCGAAATAACGCATCAATTTAATTTTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCT  >  NC_000913/3418737‑3418946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: