| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,423,383 | A→G | 22.2% | intergenic (+189/+40) | yhdZ → / ← rrfF | putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,423,383 | 0 | A | G | 22.2% | 54.0 / 11.6 | 27 | intergenic (+189/+40) | yhdZ/rrfF | putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon |
| Reads supporting (aligned to +/- strand): ref base A (11/10); new base G (3/3); total (14/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
AGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTG > NC_000913/3423264‑3423413 | agtagcgcggtggtcccacctgaccccatgccgaactcagaagtgaaacgccgtagcgccgatggtagtgtggggtctccccatgcgagagtagggaactgccagacatcaaATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCttt > 3:47975‑M1/113‑148 (MQ=255) cccacctgaccccatgccgaactcagaagtgaaacgccgtagcgccgatggtagtgtggggtctccccatgcgagagtagggaactgccagacttcaaATAAAACAAAAGGCCCAGTCTTCCGACTGAGcc > 4:1038098‑M1/99‑131 (MQ=255) cccacctgaccccatgccgaactcagaagtgaaacgccgtagcgccgatggtagtgtggggtctccccatgcgagagtagggaactgccagacatcaaCTAAAACAAAAGGCCCAGTCTTCCGACTGAGcc < 3:1038098‑M1/33‑1 (MQ=255) cGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTc > 3:661421/1‑110 (MQ=40) cGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCAGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTc < 4:661421/110‑1 (MQ=255) catgccgaactcagaagtgaaacgccgtagcgccgatggtagtgtggggtctcctcatgcgagagtagggaactgccaggcatcaaATAAAACGAAAGGCCCAGTCTTCCGACTGa > 1:491071‑M1/87‑116 (MQ=255) catgccgaactcagaagtgaaacgccgtagcgccgatggtagtgtggggtctcctcatgcgagagtagggaactgccaggcatcaaATAAAACGAAAGGCCCAGTCTTCCGACTGa < 2:491071‑M1/30‑1 (MQ=255) cTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAg < 4:71976/114‑1 (MQ=40) cTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAg > 3:71976/1‑114 (MQ=40) gggCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGa < 4:713140/105‑1 (MQ=255) gggCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGa > 3:713140/1‑105 (MQ=40) gaagtgaaacgccgtagcgccgatggtagtgtggggtctccccatgcgagagtagggaactgccagacatcaaATAAAACAAAAGGCCCAGTCTTCCGACTg < 4:881736‑M1/29‑1 (MQ=255) gaagtgaaacgccgtagcgccgatggtagtgtggggtctccccatgcgagagtagggaactgccagacatcaaATAAAACAAAAGGCCCAGTCTTCCGACTg > 3:881736‑M1/74‑102 (MQ=255) cgccgtagcgccgatggtagtgtggggtctccccatgcgagagtagggaactgccaggcatcaaATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCt < 2:358880‑M1/34‑1 (MQ=255) cgccgtagcgccgatggtagtgtggggtctccccatgcgagagtagggaactgccaggcatcaaATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCt > 1:358880‑M1/65‑98 (MQ=255) tCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGccc < 3:629843/75‑1 (MQ=255) tCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGccc > 4:629843/1‑75 (MQ=255) aaTCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCt < 2:791787/90‑1 (MQ=255) aaTCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCt > 1:691611/1‑90 (MQ=255) aaTCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCt > 1:791787/1‑90 (MQ=255) aaTCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCt < 2:691611/90‑1 (MQ=255) cccGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTg > 4:149945/1‑86 (MQ=255) cccGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTg < 3:149945/86‑1 (MQ=255) cctcatgcgagagtagggaactgccaggcatcaaATAAAACGAAAGGCCCAGTCTTCCGACTGAGcc > 3:926911‑M1/35‑67 (MQ=255) cctcatgcgagagtagggaactgccaggcatcaaATAAAACGAAAGGCCCAGTCTTCCGACTGAGcc < 4:926911‑M1/33‑1 (MQ=255) ggAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGa < 4:474855/52‑1 (MQ=255) ggAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGa > 3:474855/1‑52 (MQ=255) | AGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTG > NC_000913/3423264‑3423413 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |