Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,423,622 C→T 8.4% noncoding (34/76 nt) thrV ← tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,423,6220CT 8.4% 452.0 / 24.3 143noncoding (34/76 nt)thrVtRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base C (67/64);  new base T (7/5);  total (74/69)
Fisher's exact test for biased strand distribution p-value = 7.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

Only 100 of 145 total aligned reads displayed.

TTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGA  >  NC_000913/3423485‑3423763
                                                                                                                                         |                                                                                                                                             
ttCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTgcgc                                                                                                                                     >  2:38875/1‑148 (MQ=255)
      tCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAggg                                                                                                                                          >  2:49557/1‑137 (MQ=255)
      tCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAggg                                                                                                                                          <  1:49557/137‑1 (MQ=255)
      tCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTAcc                                                                                                                               >  1:1067552/1‑148 (MQ=255)
      tCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTAc                                                                                                                                <  3:624198/147‑1 (MQ=255)
      tCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTAc                                                                                                                                >  4:624198/1‑147 (MQ=255)
        tGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTgc                                                                                                                                       <  3:182937/138‑1 (MQ=255)
        tGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTgc                                                                                                                                       >  4:182937/1‑138 (MQ=255)
           gTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTg                                                                                                                          <  4:46636/148‑1 (MQ=255)
             tCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAg                                                                                                                        >  4:756274/1‑148 (MQ=255)
                    ggggTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATc                                                                                                                 <  1:38875/148‑1 (MQ=255)
                     gggTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTTCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCa                                                                                                                <  4:532479/148‑1 (MQ=255)
                           ggTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGc                                                                                                          >  3:352975/1‑148 (MQ=255)
                             tGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTg                                                                                                                          <  3:583191/130‑1 (MQ=255)
                              gggACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTaa                                                                                                       <  2:1067552/148‑1 (MQ=255)
                               ggACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTGGTAAGGGTg                                                                                                                                        >  1:658463/1‑114 (MQ=255)
                                 accaccGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAg                                                                                                                                            <  3:316339/108‑1 (MQ=255)
                                 accaccGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAg                                                                                                                                            >  4:316339/1‑108 (MQ=255)
                                 accaccGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAAtt                                                                                                    >  1:929084/1‑148 (MQ=255)
                                  ccaccGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCatat                                                                                                                  >  1:1038937/1‑133 (MQ=255)
                                          cTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTg                                                                                           <  3:756274/148‑1 (MQ=255)
                                                   ccAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTGGTAAGGGTga                                                                                                                                       >  4:318362/1‑94 (MQ=255)
                                                          aaTTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTGGTAAGGGTg                                                                                                                                        <  2:1010688/87‑1 (MQ=255)
                                                          aaTTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTGGTAAGGGTg                                                                                                                                        >  1:1010688/1‑87 (MQ=255)
                                                           aTTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGa                                                                                                 <  3:618162/125‑1 (MQ=255)
                                                             tCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTg                                                                                                  <  3:412437/122‑1 (MQ=255)
                                                             tCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTg                                                                                                  >  4:412437/1‑122 (MQ=255)
                                                                   cTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGcc                                                                                                                      >  4:20980/1‑96 (MQ=255)
                                                                   cTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGcc                                                                                                                      <  3:20980/96‑1 (MQ=255)
                                                                     aaTCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTGGTAAGGGTga                                                                                                                                       >  4:575631/1‑76 (MQ=255)
                                                                     aaTCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGct                                                                                                                                    <  3:56427/80‑1 (MQ=255)
                                                                     aaTCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGct                                                                                                                                    >  4:56427/1‑80 (MQ=255)
                                                                       tCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAg                                                                                                                                            <  2:620318/70‑1 (MQ=255)
                                                                            cGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCata                                                                                                                   >  4:908616/1‑90 (MQ=255)
                                                                            cGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCata                                                                                                                   <  3:908616/90‑1 (MQ=255)
                                                                            cGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAg                                                                                       >  2:140422/1‑118 (MQ=255)
                                                                            cGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAg                                                                                       <  1:140422/118‑1 (MQ=255)
                                                                            cGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCt                                                                                 >  4:862378/1‑124 (MQ=255)
                                                                            cGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCt                                                                                 <  3:862378/124‑1 (MQ=255)
                                                                                  ttAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAcccc                                                            >  4:1058205/1‑139 (MQ=255)
                                                                                  ttAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAcccc                                                            <  3:1058205/139‑1 (MQ=255)
                                                                                        tAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTcc                                                                                   >  3:846420/1‑110 (MQ=255)
                                                                                        tAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTcc                                                                                   <  4:846420/110‑1 (MQ=255)
                                                                                        tAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTActct                                                                            <  4:596278/117‑1 (MQ=255)
                                                                                        tAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTActct                                                                            >  3:596278/1‑117 (MQ=255)
                                                                                        tAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGgaga                                                                <  1:491244/129‑1 (MQ=255)
                                                                                        tAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGgaga                                                                >  2:491244/1‑129 (MQ=255)
                                                                                         aaaaaGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAggg                                                                                                                                          >  3:52082/1‑54 (MQ=255)
                                                                                         aaaaaGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTg                                                                                                                          <  4:529765/70‑1 (MQ=255)
                                                                                         aaaaaGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTg                                                                                                                          >  3:529765/1‑70 (MQ=255)
                                                                                         aaaaaGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGc                                                                                                                       <  1:412892/73‑1 (MQ=255)
                                                                                         aaaaaGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGc                                                                                                                       >  2:412892/1‑73 (MQ=255)
                                                                                         aaaaaGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACt                                                       <  1:1129041/137‑1 (MQ=255)
                                                                                         aaaaaGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGgcgc                                            >  2:209100/1‑148 (MQ=255)
                                                                                           aaaGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAcccc                                                            <  2:35304/130‑1 (MQ=255)
                                                                                           aaaGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAcccc                                                            >  1:35304/1‑130 (MQ=255)
                                                                                                  tgCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCaa                                                                                                                             <  4:499092/58‑1 (MQ=255)
                                                                                                     tGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCa                                <  2:929084/148‑1 (MQ=255)
                                                                                                      gATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGc                                                                                              >  1:853856/1‑85 (MQ=255)
                                                                                                      gATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGc                                                                                              <  2:853856/85‑1 (MQ=255)
                                                                                                      gATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCg                                     >  3:405414/1‑142 (MQ=255)
                                                                                                       aTACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAcc                                                              >  3:455249/1‑116 (MQ=255)
                                                                                                       aTACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAcc                                                              <  4:455249/116‑1 (MQ=255)
                                                                                                          cccAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCt                           >  2:1120355/1‑148 (MQ=255)
                                                                                                           ccAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTc                            >  2:926115/1‑146 (MQ=255)
                                                                                                           ccAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTc                            <  1:926115/146‑1 (MQ=255)
                                                                                                            cAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAcccc                                                            >  4:1131807/1‑113 (MQ=255)
                                                                                                            cAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAcccc                                                            <  3:1131807/113‑1 (MQ=255)
                                                                                                            cAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcaca                                                         >  3:745206/1‑116 (MQ=255)
                                                                                                            cAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcaca                                                         <  4:745206/116‑1 (MQ=255)
                                                                                                                    aaCTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGt                       >  2:858748/1‑142 (MQ=255)
                                                                                                                    aaCTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGt                       <  1:858748/142‑1 (MQ=255)
                                                                                                                        ggggACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTccc                                                                                  <  1:305085/79‑1 (MQ=255)
                                                                                                                        ggggACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTccc                                                                                  >  2:305085/1‑79 (MQ=255)
                                                                                                                         gggACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATgggg            >  3:553587/1‑148 (MQ=255)
                                                                                                                          ggACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCg                                     <  4:1101791/122‑1 (MQ=255)
                                                                                                                          ggACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCg                                     >  3:1101791/1‑122 (MQ=255)
                                                                                                                            aCCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcac                                                          >  2:258402/1‑99 (MQ=255)
                                                                                                                            aCCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcac                                                          <  1:258402/99‑1 (MQ=255)
                                                                                                                            aCCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTc                            >  1:720471/1‑129 (MQ=255)
                                                                                                                            aCCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTc                            <  2:720471/129‑1 (MQ=255)
                                                                                                                               tCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGt      >  2:716758/1‑148 (MQ=255)
                                                                                                                               tCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGt      >  3:1024140/1‑148 (MQ=255)
                                                                                                                                cACCCTTGGTAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTccc                                                                                  <  3:941496/71‑1 (MQ=25)
                                                                                                                                cACCCTTGGTAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTccc                                                                                  >  2:627424/1‑71 (MQ=25)
                                                                                                                                cACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAcc                                                              <  4:377857/91‑1 (MQ=255)
                                                                                                                                  cccTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCgg                                               >  4:37871/1‑104 (MQ=255)
                                                                                                                                  cccTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCgg                                               <  3:37871/104‑1 (MQ=255)
                                                                                                                                  cccTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTggg   <  4:1024140/148‑1 (MQ=255)
                                                                                                                                   ccTTACCAAGGGTGCGCTCTACCAACTGCGCCATATCAGCCCGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGa  >  4:585000/1‑148 (MQ=255)
                                                                                                                                   ccTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGa  >  2:1075702/1‑148 (MQ=255)
                                                                                                                                    cTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTg                                                                                                  <  4:448989/51‑1 (MQ=255)
                                                                                                                                    cTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATg                                                                                               >  3:657529/1‑54 (MQ=255)
                                                                                                                                    cTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATg                                                                                               <  4:657529/54‑1 (MQ=255)
                                                                                                                                    cTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTg                          <  2:93662/123‑1 (MQ=255)
                                                                                                                                    cTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTg                          >  1:93662/1‑123 (MQ=255)
                                                                                                                                    cTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGtt                      <  4:611280/127‑1 (MQ=255)
                                                                                                                                      tACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAcc                                                              <  3:465898/85‑1 (MQ=255)
                                                                                                                                         cAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGtt                      <  3:821112/122‑1 (MQ=255)
                                                                                                                                         cAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGtt                      >  4:821112/1‑122 (MQ=255)
                                                                                                                                         |                                                                                                                                             
TTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGA  >  NC_000913/3423485‑3423763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: