| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,477,878 | T→A | 33.3% | intergenic (+20/+29) | slyX → / ← slyD | phi X174 lysis protein/FKBP‑type peptidyl prolyl cis‑trans isomerase (rotamase) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,477,878 | 0 | T | A | 33.3% | 8.7 / 9.0 | 12 | intergenic (+20/+29) | slyX/slyD | phi X174 lysis protein/FKBP‑type peptidyl prolyl cis‑trans isomerase (rotamase) |
| Reads supporting (aligned to +/- strand): ref base T (4/4); new base A (2/2); total (6/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.69e-02 | |||||||||||
TCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGT > NC_000913/3477777‑3478010 | tCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGc < 1:1045971/109‑1 (MQ=255)tCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGc > 2:1045971/1‑109 (MQ=255) gccagccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCtt > 3:770350/1‑89 (MQ=255) gccagccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAATCCCCGCtt < 4:770350/89‑1 (MQ=255) ccagccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAt < 3:654102/79‑1 (MQ=255) ccagccagccGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGAt > 4:654102/1‑79 (MQ=255) aGCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCgtcgtggt > 1:948366/1‑141 (MQ=255) aGCGGGGAATCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCgtcgtggt < 2:948366/141‑1 (MQ=255) gggATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCt > 2:956173/1‑79 (MQ=255) gggATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGCTGCCTTTACCGCCACAGCACCCt < 1:956173/79‑1 (MQ=255) ggATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCaa < 1:1097255/126‑1 (MQ=255) ggATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCaa > 2:1097255/1‑126 (MQ=255) aTTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCa > 1:123878/1‑109 (MQ=255) aTTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCa < 2:123878/109‑1 (MQ=255) | TCTGCTGACCGAGAAGTTAAAAGCCAGCCAGCCGTCGAACATCGCGTCGCAGGCTGAAGAAACGCCACCGCCACATTATTGAGGCGTAAAAAAAGCGGGGATTCCCCGCTTTTTTGTCACTTTTTCGGTATTAGTGGCAACCGCAACCGCCGTTGCCTTTACCGCCACAGCAGCCTTCGCCACCGTGTTCATGACCGTGATCGTGGCCATGACCGCCGCAGCAACCGTCGTGGT > NC_000913/3477777‑3478010 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |