Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,488,989 G→T 5.3% H398N (CAC→AAC)  argD ← bifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,488,9890GT 5.3% 471.7 / 11.9 133H398N (CAC→AAC) argDbifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (65/61);  new base T (4/3);  total (69/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 144 total aligned reads displayed.

ACCGCATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTT  >  NC_000913/3488842‑3489129
                                                                                                                                                   |                                                                                                                                            
aCCGCATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTg                                                                                                                                              >  1:259490/1‑148 (MQ=255)
    cATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCCACGCGGTGGCg                                                                                                                                          >  4:726204/1‑148 (MQ=255)
       cACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCCACGCGGTGGCGaa                                                                                                                                        <  2:656832/147‑1 (MQ=255)
       cACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCCACGCGGTGGCGaa                                                                                                                                        >  1:656832/1‑147 (MQ=255)
           ggATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTgg                                                                                                                                             <  1:555913/138‑1 (MQ=255)
              tttGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCa                                                                                                                                <  2:465440/148‑1 (MQ=255)
                  gCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATccc                                                                                                                            <  2:259490/148‑1 (MQ=255)
                      aGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGt                                                                                                                                    <  4:245141/136‑1 (MQ=255)
                      aGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCATCGCCACCGCGTggg                                                                                                                                            >  4:740906/1‑128 (MQ=255)
                      aGTCGCAAGTTGCGGGACTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATCCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTggg                                                                                                                                            <  3:740906/128‑1 (MQ=255)
                         cGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCg                                                                                                                     >  3:216756/1‑148 (MQ=255)
                            aaGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCAt                                                                                                                       <  1:1049481/143‑1 (MQ=255)
                            aaGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCAt                                                                                                                       >  2:1049481/1‑143 (MQ=255)
                                     gATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCt                                                                                                         >  1:539691/1‑148 (MQ=255)
                                      aTTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCAt                                                                                                                       >  1:949158/1‑133 (MQ=255)
                                      aTTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCtt                                                                                                        >  3:12945/1‑148 (MQ=255)
                                           aaGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGtt                                                                                                                                   <  2:940724/116‑1 (MQ=255)
                                           aaGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGtt                                                                                                                                   >  1:940724/1‑116 (MQ=255)
                                           aaGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATccc                                                                                                                            <  3:1099442/123‑1 (MQ=255)
                                           aaGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATccc                                                                                                                            >  4:1099442/1‑123 (MQ=255)
                                           aaGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTc                                                                                                                         >  2:993450/1‑126 (MQ=255)
                                           aaGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTc                                                                                                                         <  1:993450/126‑1 (MQ=255)
                                            aGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATc                                                                                                                <  4:192335/134‑1 (MQ=255)
                                                  aaTGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCg                                                                                            >  1:743519/1‑148 (MQ=255)
                                                   aTGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGa                                                                                           <  4:12945/148‑1 (MQ=255)
                                                          cTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTggg                                                                                                                                            >  2:6051/1‑92 (MQ=255)
                                                          cTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCa                                                                                                                        >  4:501793/1‑112 (MQ=255)
                                                            aaaCCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATcc                                                                                                                             >  1:366390/1‑105 (MQ=255)
                                                            aaaCCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATcc                                                                                                                             <  2:366390/105‑1 (MQ=255)
                                                            aaaCCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCAt                                                                                                                       <  4:636801/111‑1 (MQ=255)
                                                            aaaCCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCAt                                                                                                                       >  3:636801/1‑111 (MQ=255)
                                                            aaaCCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTg                                                                                      >  4:38899/1‑144 (MQ=255)
                                                            aaaCCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTg                                                                                      <  3:38899/144‑1 (MQ=255)
                                                            aaaCCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCaaa                                                                                  >  4:1055164/1‑148 (MQ=255)
                                                              aCCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACgg                                                                                        <  2:1006081/140‑1 (MQ=255)
                                                              aCCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACgg                                                                                        >  1:1006081/1‑140 (MQ=255)
                                                                 aTCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTggg                                                                                                                                            <  4:568405/85‑1 (MQ=255)
                                                                 aTCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCa                                                                             <  3:623203/148‑1 (MQ=255)
                                                                 aTCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCa                                                                             <  2:539691/148‑1 (MQ=255)
                                                                 aTCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCa                                                                             <  1:509693/148‑1 (MQ=255)
                                                                  tCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTg                                                                                                                                              <  3:251334/82‑1 (MQ=255)
                                                                  tCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCaaa                                                                                  >  2:951021/1‑142 (MQ=255)
                                                                  tCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCa                                                                             >  1:1110573/1‑147 (MQ=255)
                                                                   cGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATc                                                                           <  3:1055164/148‑1 (MQ=255)
                                                                      aTAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACg                                                                                                                                     >  3:568209/1‑87 (MQ=255)
                                                                      aTAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCAt                                                                            <  2:93601/144‑1 (MQ=255)
                                                                      aTAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATcaca                                                                        >  1:69849/1‑148 (MQ=255)
                                                                          ggCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACAtccg                                                                    >  4:1022874/1‑148 (MQ=255)
                                                                           gCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCg                                                                                                                     >  2:252377/1‑98 (MQ=255)
                                                                           gCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCg                                                                                                                     <  1:252377/98‑1 (MQ=255)
                                                                            cATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACAt                                                                       >  4:223123/1‑143 (MQ=255)
                                                                            cATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACAt                                                                       <  3:223123/143‑1 (MQ=255)
                                                                              ttCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCAGAtcggaaga                                                                >  1:252047/1‑142 (MQ=255)
                                                                              ttCACGCCGCATCCGACATCTTTTGACTGATGCCTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCa                                                                             >  2:531374/1‑135 (MQ=255)
                                                                                  cgccgcATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGaa                                                                                                                                        <  3:704460/72‑1 (MQ=255)
                                                                                    ccgcATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCAtt                                                          <  4:665961/148‑1 (MQ=255)
                                                                                      gcATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCa                                                        <  2:743519/148‑1 (MQ=255)
                                                                                        aTCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGc                                                      <  3:1022874/148‑1 (MQ=255)
                                                                                          ccGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGc                                                                                                             <  4:1036442/91‑1 (MQ=255)
                                                                                             aCATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTc                                                                                                       <  3:1126682/94‑1 (MQ=255)
                                                                                             aCATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCa                                                                             <  1:687843/120‑1 (MQ=255)
                                                                                             aCATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCa                                                                             >  2:687843/1‑120 (MQ=255)
                                                                                             aCATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCAt                                                 >  3:1038511/1‑148 (MQ=255)
                                                                                             aCATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCAt                                                 >  1:885654/1‑148 (MQ=255)
                                                                                              cATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCa                                                                             <  1:458416/119‑1 (MQ=255)
                                                                                              cATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCa                                                                             >  2:458416/1‑119 (MQ=255)
                                                                                                 cTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGc                                                                              >  4:1071040/1‑115 (MQ=255)
                                                                                                           aTGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCaaa                                                                                  <  2:3540/101‑1 (MQ=255)
                                                                                                           aTGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCaaa                                                                                  >  1:3540/1‑101 (MQ=255)
                                                                                                            tGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACAt                                                                       >  3:875122/1‑111 (MQ=255)
                                                                                                            tGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACAt                                                                       <  4:875122/111‑1 (MQ=255)
                                                                                                            tGCTTCGTATATACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTgc                                  >  4:101440/1‑148 (MQ=255)
                                                                                                                tCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGc                                                      <  1:344168/124‑1 (MQ=255)
                                                                                                                tCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGc                                                      >  2:344168/1‑124 (MQ=255)
                                                                                                                tCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCAcc                                                   >  3:134380/1‑127 (MQ=255)
                                                                                                                tCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATGCAGCAcc                                                   <  4:134380/127‑1 (MQ=255)
                                                                                                                     tttACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATa                         >  1:61621/1‑148 (MQ=255)
                                                                                                                           ccccAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGaa                   >  4:854552/1‑148 (MQ=255)
                                                                                                                                accaccTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCAc              >  2:846834/1‑148 (MQ=255)
                                                                                                                                 ccaccTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCAcg             <  2:69849/148‑1 (MQ=255)
                                                                                                                                 ccaccTGCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCAcg             <  2:61621/148‑1 (MQ=255)
                                                                                                                                  caccTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATc                >  1:267204/1‑144 (MQ=255)
                                                                                                                                  caccTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATc                <  2:267204/144‑1 (MQ=255)
                                                                                                                                  acgtttCGCCCACGCGGTGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGaa                   >  1:804464/5‑141 (MQ=255)
                                                                                                                                  acgtttCGCCCACGCGGTGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGaa                   <  2:804464/137‑1 (MQ=255)
                                                                                                                                    ccTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCa                                                                             <  3:989349/81‑1 (MQ=255)
                                                                                                                                    ccTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCa                                                                             >  4:989349/1‑81 (MQ=255)
                                                                                                                                    ccTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACgcgc          >  3:507423/1‑148 (MQ=255)
                                                                                                                                     tttCGCCCACGCGGTGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGcc         >  1:618969/2‑148 (MQ=255)
                                                                                                                                     cTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGcc         >  4:1111604/1‑148 (MQ=255)
                                                                                                                                       tCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTa                                               >  3:68538/1‑108 (MQ=255)
                                                                                                                                       tCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTa                                               <  4:68538/108‑1 (MQ=255)
                                                                                                                                          ccACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGAcc    >  2:872434/1‑148 (MQ=255)
                                                                                                                                           cACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCAtt                                                          <  4:604962/93‑1 (MQ=255)
                                                                                                                                           cACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCAtt                                                          >  3:604962/1‑93 (MQ=255)
                                                                                                                                            aCCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAg                                                       <  3:850290/95‑1 (MQ=255)
                                                                                                                                            aCCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAg                                                       >  4:850290/1‑95 (MQ=255)
                                                                                                                                            aCCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCtt  <  1:872434/148‑1 (MQ=255)
                                                                                                                                            aCCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCtt  <  2:167566/148‑1 (MQ=255)
                                                                                                                                            aCCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCtt  <  3:108429/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
ACCGCATCACGGGATTTGGCTGAGTCGCAAGTTGCGGGATTCGAAGGCGTAATGCAGGCTAAACCATCGGATAAGGCATTCACGCCGCATCCGACATCTTTTGCCTGATGCTTCGTATTTACGCCCCAACCACCTTCGCCACCGCGTGGGCGAAACGTTGCATCCCTTCATCGATATCCGCATCTTCCACCACCAGCGACGGTGCAAAACGCATCACATCCGGTCCGGCATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTT  >  NC_000913/3488842‑3489129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: