Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,092,774:1 | +A | 6.7% | coding (897/951 nt) | gshB → | glutathione synthetase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,092,774 | 1 | . | A | 6.7% | 709.2 / 49.5 | 179 | coding (897/951 nt) | gshB | glutathione synthetase |
Reads supporting (aligned to +/- strand): ref base . (85/82); new base A (8/4); total (93/86) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.76e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-02 |
Only 100 of 188 total aligned reads displayed.
AACGCGTGCTGGTGGTGGATGGCGAGCCGGTACCGTACTGCCTGGCGCGTATTCCGCAGGGGGGCGAAACCCGTGGCAATCTGGCTGCCGGTGGTCGCGGTGAACCTCGTCCGCTGACGGAAAGTGACTGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTGCTCTCCAGGATCCGATTTTCCGTCGTTCCGTGGTCTACATTTGCGAACATAATACCAATGGTGCAATGGGGA > NC_000913/3092503‑3093045 | tGCTGGTGGTGGATGGCGAGCCGGTACCGTACTGCCGGGCGCGTATTCCGCAGGGGGGCGAAACCCGTGGCAATCTGGCTGCCGGTGGTCGCGGTGAACCTCGTCCGCTGACGGAAAGTGACTGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCAc < 2:297216/274‑1 (MQ=255) gggggtggtGGGCGATCCGGTACCGGACTGCGGGGCGCGTATGCCGCAGGGGGGGGAAACCCGTGGCAATCTGGCGGCCGGTGGTCGCGGTGAACCTCGTCCGCTGACGGAAAGTGACTGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGa < 4:119449/271‑1 (MQ=255) ggtggATGGCGAGCCGGTACCGTACTGCCTGGCGCGTATTCCGCAGGGGGGCGAAACCCGTGGCAATCTGGCTGCCGGTGGTCGCGGTGAACCTCGTCCGCTGACGGAAAGTGACTGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATg > 2:689252/1‑274 (MQ=255) tGGCGAGCCGGTACCGTACTGCCTGGCGCGTATTCCGCAGGGGGGCGAAACCCGTGGCAATCTGGCTGCCGGTGGTCGCGGTGAACCTCGTCCGCTGACGGAAAGTGACTGGAAAATCGCACGGCAGATCGGTCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTTGTCTGGATCTCATTGGCGACAGTATGACTGCAATTAACGTCACTAGCACAACCTGTAGTCGGGAGATTGAGTACAAGTCTCCGTTG‑TCCATCACCGGCATTTTCAtt > 4:508937/1‑273 (MQ=255) cGTACTGCATGGGGCGGATTCCGCAGGGGGGCGAAACCCGTGGCAAGCTGGCTGCCGGTGGTCGCGGTGAACCTCGTCGGCTGACGGAAAGTGACTGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGc < 2:530951/274‑1 (MQ=255) ccTGGCGCGTATTCCGCAGGGGGGCGAAACCCGTGGCAATCTGGCTGCCGGTGGTCGCGGTGAACCTCGTCCGCTGACGGAAAGTGACTGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGgtg‑t > 4:226735/1‑233 (MQ=255) gcggggtggccGCGGGGGGGCGGAACCCGTGGACAGAGGGCGGCGGGTGGCGGCGGGGAACCTAGTCCGATGACGGAAAGGGACGGGAAAATCTCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGAGTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGGGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTAcagc < 4:27620/265‑1 (MQ=255) gcgcGTATTCCGCAGGGGGGCGAAACCCGTGGCAATCTGGCTGCCGGTGGTCGCGGTGAACCTCGTCCGCTGACGGAAAGTGACTGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGAt < 2:226736/233‑1 (MQ=255) gTATTCCGCAGGGGGGCGAAACCCGTGGCAATCTGGCTGCCGGTGGTCGCGGTGAACCTCGTCCGCTGACGGAAAGTGACTGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTAcagcagcag < 2:62425/275‑1 (MQ=255) aTTCCGCAGGGGGGCGAAACCCGTGGCAATCTGGCTGCCGGTGGTCGCGGTGAACCTCGTCCGCTGACGGAAAGTGACTGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATAGCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGTA‑TGCAATCGCAGCACGTTTACAGCAGCAGTa > 4:642665/1‑275 (MQ=255) gCAGGGGGGCGAAACCCGTGGCAATCTGGCTGCCGGTGGTCGCGGTGAACCTCGTCCGCTGACGGAAAGTGACTGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTTTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGCTTGAAGCAACATTTCCGGTT‑TCGATCACCGGAATGTTAATGGACTCCCTTCG‑AGCACGTTTCAAGCAGCCGTAACCCa > 4:103471/1‑275 (MQ=255) cgGGGAACCACGTCCGCTGACGGAAAGTGACTGGAAAATCGCCCGTCAGGTCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTTGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAgcg < 2:655734/274‑1 (MQ=255) gAACCTCGTCCGCTGACGGAAAGTGACTGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGccc > 2:662189/1‑273 (MQ=255) aCGGAAAGTGACTGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCCAAGCACGTTTACAGCCGCAGTAACCCACCTTAGCGAGAAGGACCTCGTTGAGCCTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACt > 2:514068/1‑275 (MQ=255) aCTGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGT‑TGCCa > 4:478057/1‑174 (MQ=255) cTGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGa < 8:439909/149‑1 (MQ=255) tGGAAAATCGTCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGa < 5:252180/148‑1 (MQ=255) gggAAAACCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGAt < 8:484504/148‑1 (MQ=255) gAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGa < 2:478059/174‑1 (MQ=255) gAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCa < 2:156989/253‑1 (MQ=255) tCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTa > 5:763388/1‑148 (MQ=255) cAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGgtg‑t > 5:800018/1‑130 (MQ=255) cAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGgtg‑t < 6:800018/130‑1 (MQ=255) aTCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATgg > 6:299782/1‑148 (MQ=255) tCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGaa < 7:480263/138‑1 (MQ=255) cGGGCAGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGAATGAAATTAACGTCACCAGCCCAACCTGTAGTCGTGAGATTGAAGCAGAGTGTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGAACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCGTTCCACGC‑ATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAg < 1:108627/275‑1 (MQ=255) ggCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGc < 5:113990/148‑1 (MQ=255) gCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTNATGGA‑TGCCATCGACGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTGGTTGAGCATCTGATGGCCACCNTTCTTCTTTCCCAGCAATACGGGGTNGT‑TTGCTTTTTTTAAACAGGAAACAGaaa > 3:145828/1‑274 (MQ=255) ccGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCa < 5:433456/148‑1 (MQ=255) cGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCAt > 6:384406/1‑148 (MQ=255) gACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGgtg‑t > 6:429824/1‑119 (MQ=255) agaaaAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGtt < 8:297657/149‑1 (MQ=255) aaaaaGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTa < 7:182284/149‑1 (MQ=255) aaaGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAAtt < 2:320470/274‑1 (MQ=255) ggCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTAcagcag > 8:581623/1‑149 (MQ=255) ggCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTATATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTAc > 2:135004/1‑273 (MQ=255) gATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCGGACAATGAATTTAAAGCATc > 2:59651/1‑275 (MQ=255) tttttGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTaa > 7:455450/1‑149 (MQ=255) ttttGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTAcagca > 8:151178/1‑141 (MQ=255) ttttGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTaa > 6:750175/1‑148 (MQ=255) ttttGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTaa > 5:718432/1‑148 (MQ=255) tCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAg > 7:784137/1‑149 (MQ=255) ggATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCgag < 4:749098/150‑1 (MQ=255) gATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCgag > 8:882249/1‑149 (MQ=255) gATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCAt > 2:342755/1‑275 (MQ=255) atcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGa < 5:384406/148‑1 (MQ=255) atcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTGc > 1:110089/1‑275 (MQ=255) cGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGAtc > 5:819075/1‑148 (MQ=255) gCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCAc > 2:359924/1‑79 (MQ=255) gCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGc > 2:770989/1‑202 (MQ=255) gCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTGCtctc < 4:141274/275‑1 (MQ=255) ccGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGa < 4:359922/79‑1 (MQ=255) ctgaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTg < 5:750175/148‑1 (MQ=255) gaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGActc > 5:420898/1‑144 (MQ=255) aTTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGa > 5:23846/1‑66 (MQ=255) aTTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGAc > 8:222908/1‑149 (MQ=255) ttAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACa > 8:801000/1‑149 (MQ=255) ttAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACa < 7:801000/149‑1 (MQ=255) ttAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACa < 7:890580/149‑1 (MQ=255) gTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTGCTCTCCAGGATCCGATTTTCCGTCGTTc < 1:208011/275‑1 (MQ=255) cACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGccc < 6:763388/148‑1 (MQ=255) ccAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGa < 7:700050/117‑1 (MQ=255) aGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGa < 5:686376/115‑1 (MQ=255) cccAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCttt < 6:824614/148‑1 (MQ=255) aaCCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCAc < 7:680747/149‑1 (MQ=255) aCCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTg > 2:40800/1‑163 (MQ=255) ccTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGtt > 8:690031/1‑115 (MQ=255) ccTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTGCTCTCCAGGATCCGATTTTCCGTCGTTCCGTGGTCTACAt > 2:390181/1‑274 (MQ=255) tATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTGCTCTCCAGGATCCGATTTTCCGTCGTTCCGTGGTCTACATTTGCg < 2:109720/275‑1 (MQ=255) tCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTgg < 8:281053/149‑1 (MQ=255) tCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTgg < 7:388103/149‑1 (MQ=255) tCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTgg < 7:257623/149‑1 (MQ=255) tCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTg < 5:299782/148‑1 (MQ=255) gTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCTTTTTTGa > 1:66535/1‑165 (MQ=255) gTGAGATTGAAGCAGAGTTTCCGCTG‑TCGCTCACCGGTATGTTAATGGC‑TGCCCTCGCAGCACGTTTACAGCTGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGCGTCCCAGCTCCCTTCTTTCCACCC‑ATACTGGCCCCTGTTGCTTTTTTGAACCAGGCACCAGCACCTCTGACCATGAATTTACAGCATCACTTTCTTCTTGCCATGCCTGCTCTCCAGGATCCTATTTTCCTTCTTTCCGTGGTCTCCAttttctacct > 4:162903/1‑267 (MQ=255) tGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGGTCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGgc > 6:666479/1‑148 (MQ=255) tGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTGCTCTCCGGGATCCGATTTTCCGTCGTTCCGTGGTCTACATTTGCGAACat < 2:508939/274‑1 (MQ=255) ggggaTTGAAGCAGGTTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑GTACTGGGCGCTATTGCTTTTTTGAGCCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTGCTCACCAGGATCCGATTTTCCGTCGTGCCGTGGTCTACATTTGCGAACat < 4:390179/271‑1 (MQ=255) gagaTTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCAATGCCTGCCCTCCAGGATCCGATTTTCCGTCGTTCCGTGGTCTCAATTTGCGAACatta > 4:684647/1‑273 (MQ=255) ttGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGtt > 8:356177/1‑149 (MQ=255) ttGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGt > 6:92107/1‑148 (MQ=255) ttGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGt > 5:340317/1‑148 (MQ=255) aaGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCAc < 3:36796/54‑1 (MQ=255) aaGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCt > 7:132279/1‑149 (MQ=255) aGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGa < 7:843195/106‑1 (MQ=255) gCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTGCTCTCCAGGATCCGATTTTCCGTCGTTCCGTGGTCTACATTTGCGAACATAATACCAATgg < 4:342753/275‑1 (MQ=255) ttaacattCCGGTGATCGA‑CACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGtt > 8:787823/7‑143 (MQ=255) cAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCttt > 6:392856/1‑148 (MQ=255) gagTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCtttttt < 7:414766/149‑1 (MQ=255) gagTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCAGTTGCtttttt < 7:530032/149‑1 (MQ=255) attttCCCGTG‑TCGATCTCCGGAATGTTTNNGGG‑TGCCATCGTAGCACGTTTACAGCCGGANTAACCCCCCCTAGCGAGAAGGATCTCGTTGAGACTCTGGGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCNGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTGCTCTCCAGGATCCGATTTTCCGTCGTTCCGTGGTCTACATTTGCGAACATAATACCAATGGTGCa < 3:110088/273‑1 (MQ=255) agTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTg > 2:447768/1‑170 (MQ=255) attCCGGTGATCGA‑CACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCga > 7:800570/2‑76 (MQ=38) ttCCGGTGATCGA‑CACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCGGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCTTTTTTGaa > 6:228291/1‑148 (MQ=255) ttCCGGTGATCGA‑CACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCTTTTTTGaa > 5:178791/1‑148 (MQ=255) tCCGGTGATCGA‑CACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTAcagcagca < 7:286940/57‑1 (MQ=37) tCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGtt < 4:446829/87‑1 (MQ=255) tCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGAct < 4:276264/93‑1 (MQ=255) ccGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACa > 4:658460/1‑156 (MQ=255) tg‑tCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGT‑ATACTGGGCGCTGTTGCTTTTTTGAACCAGGaa > 8:596929/1‑149 (MQ=255) | AACGCGTGCTGGTGGTGGATGGCGAGCCGGTACCGTACTGCCTGGCGCGTATTCCGCAGGGGGGCGAAACCCGTGGCAATCTGGCTGCCGGTGGTCGCGGTGAACCTCGTCCGCTGACGGAAAGTGACTGGAAAATCGCCCGTCAGATCGGGCCGACGCTGAAAGAAAAAGGGCTGATTTTTGTTGGTCTGGATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTG‑TCGATCACCGGAATGTTAATGGA‑TGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGC‑ATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTGCTCTCCAGGATCCGATTTTCCGTCGTTCCGTGGTCTACATTTGCGAACATAATACCAATGGTGCAATGGGGA > NC_000913/3092503‑3093045 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |