| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,652,133 | A→C | 5.1% | V52G (GTG→GGG) | yfhM ← | bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,652,133 | 0 | A | C | 5.1% | 498.5 / 12.7 | 152 | V52G (GTG→GGG) | yfhM | bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (62/79); new base C (8/2); total (70/82) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01 | |||||||||||
Only 100 of 154 total aligned reads displayed.
CCTCCTTGCCAATAGTAACGATCAAATCACGTTTCGGTTCGAGGTGGCGTAAACGTAACTCTTTAAGATTATCTGACAGCTCCCAGGCACCATCCACTTTGCCGCTTTTTTTATCGACGACATGAATAACGCGTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCC > NC_000913/2651861‑2652383 | cctccgTTCCAAGAGTAACGATCAAATCACGTTTCGGTTCGGGGGTGCGTAAACGTAACTCTTTAAGATTATCTGACAGCTCCCAGGCACCATCCACTTTGCCGCTTTTTTTATCGACGACATGAGTAACGAGTGAGAAAGCCTGATCCGGGCCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACg < 4:50955/275‑1 (MQ=255) tAGTAACGATCAAATCACGTTTCGGTTCGAGGTGGCGTAAACGTAACTCTTTAAGATTATCTGACAGCTCCCAGGCACCATCCACTTTGCCGCTTTTTTTATCGACGACATGAATAACGCGTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGCAATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTTCTTCTCCGGCCGGGAGTGTTTTGCt > 4:220045/1‑275 (MQ=255) gTAACGATCAAATCACGTTTCGGTTCGAGGTGGCGTAAACGTAACTCTTTAAGATTATCTGACAGCTCCCAGGCACCATCCACTTTGCCGCTTTTTTTATCGACGACATGAATAACGCGTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCGGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGATAGTTTTGCTGa > 2:179622/1‑275 (MQ=255) ggTTCGAGGTGGCGTAAACGTAACTCTTTAAGATTATCTGACAGCTCCCAGGAACCATCCACTTTGCCGCTTTTTTTATCGACGACATGAATAACGCGTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGc < 2:192560/275‑1 (MQ=255) aCAGCTCCCAGGCACCATCCACTTTGCCGCTTTTTTTATCGACGACATGAATAACGCGTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTcgc < 1:163617/220‑1 (MQ=255) cTCCCAGGCACCATCCACTTTGCCGCTTTTTTTATCGACGACATGAATAACGCGTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGaa < 4:109725/226‑1 (MQ=255) cccAGGCACCATCCACTTTGCCGCTTTTTTTATCGACGACATGAATAACGCGTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACTGGACCTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGCGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCACTTTAGTAACTTCACAAGGCCCATCTTTTTTACCCCATGTTGgc > 4:73984/1‑275 (MQ=255) ccAGGCACCATCCACTTTGCCGCTTTTTTTATCGACGACATGAATAACGCGTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGc > 4:158996/1‑228 (MQ=255) gCACCATCCACTTTGCCGCTTTTTTTATCGACGACATGAATAACGCGTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTTTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCt < 2:158997/228‑1 (MQ=255) tCCACTTTGCCGCTTTTTTTATCGACGACATGAATAACGCGTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAAGCGCATCTTTTTTACCCGCTGTTGGCGCGTTATCgt > 2:45281/1‑274 (MQ=255) cACTTTGCCGCTTTTTTTATCGACGACATGAATAACGCGTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGGCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACATTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTGTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCgtt < 2:300919/273‑1 (MQ=255) gCCGCTTTTTTTATCGACGACATGAATAACGCGTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGACGGCGAATCTTTTTTCACCTCTGTTGGCCAGTTATCGTTTTTTTCGc > 4:268307/1‑275 (MQ=255) gCCGCTTTTTTTATCGACGAAATGAATAACGCGTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCCGCACCAGCGTGCCTGCACCATCAAGTTTGCCTTCAGAGAGCTCCAGCAATGTCCGCACCTTACCGGAACTCCGTTAGGCAAGTTTTTCTCTCTCCGGCCCCGAGCGTTTTGCTCAACTTGCCTTTTCTGCAGCGGCCGCTTTCGTAACTTCATCATGCGCATTTTTTTTCACCCCTTTTCGCCCTTTATCTTTGTTTTCg > 4:235189/1‑274 (MQ=255) tCGACGACATGAATAACGCGGGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTgccagcgc < 2:73985/275‑1 (MQ=255) aCGCGTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACgg > 6:238389/1‑148 (MQ=255) aCGCGTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGga < 7:620438/149‑1 (MQ=255) cgTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCNGTTTTTGTCTCTCCGGCACGGAGNGtttn > 4:718664/1‑152 (MQ=255) cgTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGgaga > 6:138657/1‑148 (MQ=255) gTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGTGCCGGagag < 5:48040/148‑1 (MQ=255) tGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGtt < 8:217896/149‑1 (MQ=255) tGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGt < 6:868443/148‑1 (MQ=255) aaaTCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTg < 5:744060/148‑1 (MQ=255) aaTCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGc < 6:468763/148‑1 (MQ=255) acccTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTg > 7:510685/3‑149 (MQ=255) cGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGACGAGGc > 3:200733/1‑162 (MQ=255) gTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACt > 2:575141/1‑172 (MQ=255) tCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGtttt < 5:600757/148‑1 (MQ=255) tCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCt < 1:200734/162‑1 (MQ=255) agagGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAg < 4:575139/172‑1 (MQ=255) agagGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTgccagcgc < 2:486346/233‑1 (MQ=255) gagGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGaa < 8:340605/149‑1 (MQ=255) gggATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAag < 6:526182/148‑1 (MQ=255) gggATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAag < 5:781380/148‑1 (MQ=255) ggATGGGCAACGGCAGACGCAGCGTGGCTGCACGATCAAGTTGACATTCAGATGTATCCCGCAATTTCAGTGCCTTACCGGCACTGTGTTGTGCCAGTTTTTGTCTCTCCGGCACGGCGAGTTTTGCTGAACTTGCGTTTTCTGAGGTGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTGTCGCCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTttgtcagttttcat < 4:179621/275‑1 (MQ=255) ggATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAagag > 7:390687/1‑149 (MQ=255) tGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCTGTTTTTGTCTCTCCGGCACGGAGCGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAGGGCGCACCTTTTTTCACCGCTTTTGGCGCTTTTTTTTTGTTTTCGCCCCCTGCCCGCGCTAGCATTGGCATGTAGGCGGCTCCGCGTAACTtttttatttttgattc > 4:10634/1‑272 (MQ=255) tGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAag > 5:709251/1‑144 (MQ=255) tGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAag < 6:709251/144‑1 (MQ=255) ggAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGcc > 5:470156/1‑148 (MQ=255) gTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCa < 7:482614/103‑1 (MQ=255) gTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGt > 8:805695/1‑149 (MQ=255) taaGCACCAGCGTGGCTGCACCATCAAGTTAGACTTCAGAGAGTTCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACaa < 2:10634/272‑1 (MQ=255) tCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGt > 6:584153/1‑148 (MQ=255) cAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACt > 5:183460/1‑120 (MQ=255) aGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAAc > 8:50948/1‑149 (MQ=255) aGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTTGGCCAGTTTTGGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAAATTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTgccagcgc < 2:736878/209‑1 (MQ=255) gCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGa < 6:795763/148‑1 (MQ=255) gCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACACTGGCCCGTTa > 2:171153/1‑275 (MQ=255) tGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAgg < 5:324577/148‑1 (MQ=255) cTGCACCATCAAGTTGGACTTCAGAGGGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACg < 2:228985/275‑1 (MQ=255) gCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATc > 8:871521/1‑149 (MQ=255) cACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCGCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTg < 1:223929/250‑1 (MQ=255) cACCAGCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCg < 2:688572/275‑1 (MQ=255) aCCATCAAGTTTGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCtt > 7:527779/1‑149 (MQ=255) gTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCttt > 7:384676/1‑118 (MQ=255) gTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCttt < 8:384676/118‑1 (MQ=255) ttGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGTGCCGGagag > 5:630974/1‑81 (MQ=255) gACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCAAAATGGCCCGTTAGCAACGTCGAATAATTATTATg > 2:100103/1‑275 (MQ=255) ttCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGtt < 6:245165/148‑1 (MQ=255) gagagaTCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGgcgc < 8:527779/149‑1 (MQ=255) agagaTCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGcgcg > 7:509589/1‑149 (MQ=255) gagaTCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAAt < 2:268308/275‑1 (MQ=255) cAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATc > 5:693072/1‑147 (MQ=255) cAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATc < 6:693072/147‑1 (MQ=255) cAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCCGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGGTAGCAACGTCGAATAATTATTATGGGTGAGACttttagt > 4:686182/1‑271 (MQ=255) aaTGTCAGAACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAAt < 2:686184/265‑1 (MQ=255) gTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTtgttgt < 5:238389/148‑1 (MQ=255) cAGCACCTTACCGGCATTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGAGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAAtt < 2:589842/275‑1 (MQ=255) gCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCAcc > 5:755003/1‑119 (MQ=255) cACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGt > 6:199448/1‑131 (MQ=255) cACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGt < 1:65805/187‑1 (MQ=255) ttgccGGTACTCGGGTTGGCAAGTTTTGCTATTTCGGGCGAGGAGAGTCTTGCTGAACTCACGTTTGCTAATGAGGCGGCGTTAGTTATGTCAAAAGGCGACTCTTTTGGTACCGCTGTTGGCGCTGTATCGTATTGGTAGAGCTGTGCCAGTGACAGCATTAGCATGCAGGCGGCTACGCGGACATTTTGCATTTTTCATCCCTTCCACAGTGTCGCGTTAGCAACGTCGGAGAAGTATTATGCGTTAGAATTTACGTTATGTAATTCAGTAt < 4:151401/271‑1 (MQ=255) tACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTgc > 2:111612/1‑148 (MQ=255) tccGGCAATCGTTTGTGCCAGTTTTTTTCTGTCCGGCACGGAGATTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCCCCGCTGTTGGCGCGTTATCGTTGTTGTCGCAACCGGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTAt < 4:525071/271‑1 (MQ=255) ggCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCg < 2:475161/226‑1 (MQ=255) gCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCg > 3:118293/1‑135 (MQ=255) cACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCgttg < 6:628898/128‑1 (MQ=255) cACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTgccagcgcc < 8:510685/149‑1 (MQ=255) tctGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCAcc < 1:118294/135‑1 (MQ=255) tctGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCt < 7:590957/137‑1 (MQ=255) tctGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCt > 8:590957/1‑137 (MQ=255) tGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCCGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACTACGAATAATTATTATGCGTTAGAATTTACGTTATGTAATTCAGTCACACTCCCCTAcct > 4:70691/1‑273 (MQ=255) gTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAAt < 2:22809/275‑1 (MQ=255) ttGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTa < 8:390687/149‑1 (MQ=255) gggCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTAt < 2:70692/258‑1 (MQ=255) gCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCa < 6:554542/148‑1 (MQ=255) ccAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATg > 7:297549/1‑149 (MQ=255) gTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCttttt > 2:458898/1‑87 (MQ=255) gTTTTTGGCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTAGCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACttttt < 1:133604/169‑1 (MQ=255) tttGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAggc < 5:584153/148‑1 (MQ=255) ttGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCAc < 4:458896/87‑1 (MQ=255) aactctcCGTGCCGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAggcggc > 6:13312/3‑148 (MQ=255) aactctcCGTGCCGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCt > 7:103975/3‑149 (MQ=255) actctcCGTGCCGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCt > 5:418355/2‑148 (MQ=255) ctctcCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCACCGCCAGCATTAGCATGCAGGCGGCTAc > 7:812937/1‑149 (MQ=255) tctcCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCgtt < 7:94243/103‑1 (MQ=255) tctcCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTAcg > 7:298937/1‑149 (MQ=255) ctcCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTAcgc < 8:509589/149‑1 (MQ=255) ggCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGTCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTaa < 7:871521/149‑1 (MQ=255) gCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTaa > 6:320970/1‑148 (MQ=255) | CCTCCTTGCCAATAGTAACGATCAAATCACGTTTCGGTTCGAGGTGGCGTAAACGTAACTCTTTAAGATTATCTGACAGCTCCCAGGCACCATCCACTTTGCCGCTTTTTTTATCGACGACATGAATAACGCGTGAGAAATCCTGATCCGGGTCGAGAGGGATGGAGAACGTCAGCACCAGCGTGGCTGCACCATCAAGTTGGACTTCAGAGAGATCCAGCAATGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATACAATCC > NC_000913/2651861‑2652383 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |