Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 314,255 T→C 8.4% intergenic (‑450/‑102) ykgP ← / → eaeH pseudogene, oxidoreductase family/pseudogene, attaching and effacing protein homology;factor; Not classified

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913314,2550TC 8.4% 488.2 / 21.8 154intergenic (‑450/‑102)ykgP/eaeHpseudogene, oxidoreductase family/pseudogene, attaching and effacing protein homology;factor; Not classified
Reads supporting (aligned to +/- strand):  ref base T (74/66);  new base C (7/6);  total (82/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 156 total aligned reads displayed.

CTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTCAGTTCTGGCCCGCTGCGTGGCGTGGGCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCAT  >  NC_000913/314006‑314526
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                               
cTATCTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCCCTATGAATAGTGATTTATTCACTGTCCGCAGAGTATGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAATGCTAAATTTTCCCCCATAATTAAAAATATAATCCCGCGCAAAACCACCTGATGAGTAGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTAACTTAAGGTATTTCCTTTTCTGGGGAAAGGGAACTAGGGAAATgcc                                                                                                                                                                                                                                                        >  4:292178/1‑275 (MQ=255)
                                                                                       aaGAAATATAACTTAGGTATCTATTTAATGACTTTCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCTCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCATTTTCTTACATAAAGGTATTTCCTTTTCTGCGGACAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTTTTCAGGAAATTATCTCATTGTCACATta                                                                                                                                                                 >  2:711364/1‑275 (MQ=255)
                                                                                                         aTCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCa                                                                                                                                                                                                                               <  4:398382/195‑1 (MQ=255)
                                                                                                             aTTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGaaa                                                                                                                                                                                                                                                                          <  5:471712/148‑1 (MQ=255)
                                                                                                             aTTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGaaa                                                                                                                                                                                                                                                                          <  6:16155/148‑1 (MQ=255)
                                                                                                             aTTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGaaa                                                                                                                                                                                                                                                                          <  6:805871/148‑1 (MQ=255)
                                                                                                             aTTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCCGCAGaaa                                                                                                                                                                                                                                                                          <  5:448143/148‑1 (MQ=255)
                                                                                                              tttAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGaaaa                                                                                                                                                                                                                                                                         <  5:343886/148‑1 (MQ=255)
                                                                                                                tAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCCGCAGAAAAGGa                                                                                                                                                                                                                                                                      <  7:6546/149‑1 (MQ=255)
                                                                                                                 aaTGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCCGCAGAAAAGGaa                                                                                                                                                                                                                                                                     <  7:731095/149‑1 (MQ=255)
                                                                                                                       ttGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCgg                                                                                                                                                                                                                                                                <  8:225329/148‑1 (MQ=255)
                                                                                                                       ttGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCgg                                                                                                                                                                                                                                                                >  7:225329/1‑148 (MQ=255)
                                                                                                                        tGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGt                                                                                                                                                                        >  2:1235/1‑235 (MQ=255)
                                                                                                                        tGCACAAAAAGCTAAACTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTTCGGAAAAGGAAATCggg                                                                                                                                                                                                                                                               <  6:691695/148‑1 (MQ=255)
                                                                                                                               aaaGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATcccc                                                                                                                                                                                                                                                       >  7:708690/1‑149 (MQ=255)
                                                                                                                                aaGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCg                                                                                                                                                                                                                                                      >  8:805325/1‑149 (MQ=255)
                                                                                                                                aaGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCg                                                                                                                                                                                                                                                      <  8:341593/149‑1 (MQ=255)
                                                                                                                                    tAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCCGCAGAAAAGGAAAtaccttt                                                                                                                                                                                                                                                             >  7:420357/1‑132 (MQ=255)
                                                                                                                                    tAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCCGCAGAAAAGGAAAtaccttt                                                                                                                                                                                                                                                             <  8:420357/138‑7 (MQ=255)
                                                                                                                                       aTTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGttttt                                                                                                                                                                                                                                                <  6:303926/148‑1 (MQ=255)
                                                                                                                                           tCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGAc                                                                                                                                                                                                                                   >  2:661106/1‑157 (MQ=255)
                                                                                                                                               ccATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGc                                                                                                                                                                                                                                       <  7:678681/149‑1 (MQ=255)
                                                                                                                                               ccATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCa                                                                                                                                                                                                                               <  4:661104/157‑1 (MQ=255)
                                                                                                                                               ccATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTAACAAGc                                                                                                                                                                                                                                       >  8:644206/1‑149 (MQ=255)
                                                                                                                                                  taaataaaAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGa                                                                                                                                                                                                                                    >  7:869052/1‑149 (MQ=255)
                                                                                                                                                     ataaaAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACaa                                                                                                                                                                                                                                         >  8:346349/1‑141 (MQ=255)
                                                                                                                                                     ataaaAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACaa                                                                                                                                                                                                                                         <  7:346349/141‑1 (MQ=255)
                                                                                                                                                         aaaTATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGAc                                                                                                                                                                                                                                           >  8:260163/1‑135 (MQ=255)
                                                                                                                                                         aaaTATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGAc                                                                                                                                                                                                                                           <  7:260163/135‑1 (MQ=255)
                                                                                                                                                         aaaTATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCatt                                                                                                                                                                                                                             >  7:130897/1‑149 (MQ=255)
                                                                                                                                                         aaaTATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTCAGTTCTGGCCCACTGCGTTGCGTGGGCaaa                                                                                               >  1:179753/1‑275 (MQ=255)
                                                                                                                                                           atatAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCattat                                                                                                                                                                                                                           >  7:890577/1‑149 (MQ=255)
                                                                                                                                                            tataATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCattatt                                                                                                                                                                                                                          >  7:190815/1‑149 (MQ=255)
                                                                                                                                                               aaTCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTtgt                                                                                                                                                                                                                       <  8:890577/149‑1 (MQ=255)
                                                                                                                                                                aTCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCgg                                                                                                                                                                                                                                                                <  7:901242/107‑1 (MQ=255)
                                                                                                                                                                 tCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCattat                                                                                                                                                                                                                           >  4:726548/1‑143 (MQ=255)
                                                                                                                                                                 tCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAAGCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCGCAATGTCACATTATAAAACAGTTCATAAACAACCACGATTTCGTTATTCAGTTCTGGCCCGCGTCGTGGCTTGGGCAAATATctctt                                                                                       >  4:536292/1‑274 (MQ=255)
                                                                                                                                                                     gcgcCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGc                                                                                                                                                                                                                 <  8:869052/149‑1 (MQ=255)
                                                                                                                                                                      cgcCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACaa                                                                                                                                                                                                                                         >  4:24178/1‑124 (MQ=255)
                                                                                                                                                                      cgcCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGc                                                                                                                                                                                                                                       >  4:802564/1‑126 (MQ=255)
                                                                                                                                                                      cgcCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCCATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTCAGTTCTGGCCCGCTGCGTGGCTTggg                                                                                                   >  2:99416/1‑258 (MQ=255)
                                                                                                                                                                      cgcCCAACCACCTGATGAGTGGCTATAGGCACTGGACATATCAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTGTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAACTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATCCAGTTCGGGACCCATGCGTGCGCTGGCCAAattgccatgttca                                                                                   >  4:712025/1‑263 (MQ=255)
                                                                                                                                                                        cccAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAAt                                                                                                                                                                          >  2:503119/1‑185 (MQ=255)
                                                                                                                                                                          cAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAg                                                                                                                                                                                                                                     <  2:24178/124‑1 (MQ=255)
                                                                                                                                                                            accaccTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTca                                                                                                                                                                      <  4:503117/185‑1 (MQ=255)
                                                                                                                                                                                   gatgaGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTCAGTTCTGGCCCGCTGCGTGGCGTGGGAAAATAGCTCTGTTCAGGTTCTTTTTTCAc                                                                     >  1:97318/1‑275 (MQ=255)
                                                                                                                                                                                   gatgaGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTATTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTaa                                                                                                                                                                                                    >  5:267394/1‑148 (MQ=255)
                                                                                                                                                                                     tgaGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAAt                                                                                                                                                                          >  3:187827/1‑172 (MQ=255)
                                                                                                                                                                                         tGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTca                                                                                                                                                                      <  1:187828/172‑1 (MQ=255)
                                                                                                                                                                                         tGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTCCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTtc                                                                                                                                                                                             <  7:288656/149‑1 (MQ=255)
                                                                                                                                                                                            cTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCatta                                                                                                                                                                                                                            <  8:690754/115‑1 (MQ=255)
                                                                                                                                                                                             tataGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCAGCGGAAAAGGAAATCGGGTAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTtcatca                                                                                                                                                                                         >  8:419129/1‑149 (MQ=255)
                                                                                                                                                                                              ataGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAg                                                                                                                                                                                        >  7:588228/1‑149 (MQ=255)
                                                                                                                                                                                                     cTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAAtt                                                                                                                                                                                  <  6:185666/148‑1 (MQ=255)
                                                                                                                                                                                                      tGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGAc                                                                                                                                                                                                                                   >  3:57537/1‑98 (MQ=255)
                                                                                                                                                                                                      tGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAAt                                                                                                                                                                          >  4:395828/1‑155 (MQ=255)
                                                                                                                                                                                                        gATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTAtct                                                                                                                                                                              >  7:169192/1‑149 (MQ=255)
                                                                                                                                                                                                          tatatTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCATGAAATTATCTCAATGTca                                                                                                                                                                      <  2:475792/155‑1 (MQ=255)
                                                                                                                                                                                                          tatatTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTca                                                                                                                                                                      <  2:395830/155‑1 (MQ=255)
                                                                                                                                                                                                              ttAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGt                                                                                                                                                                        >  7:629585/1‑149 (MQ=255)
                                                                                                                                                                                                               tAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGcc                                                                                                                                                                                                                <  5:806885/108‑1 (MQ=255)
                                                                                                                                                                                                                 ggTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCgg                                                                                                                                                                                                                                                     <  7:635731/69‑1 (MQ=255)
                                                                                                                                                                                                                 ggTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTAGGTTCTTTAATTCGTTCATCAGGAAATTATCTCAATGTcac                                                                                                                                                                     >  8:587730/1‑149 (MQ=255)
                                                                                                                                                                                                                  gTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAAt                                                                                                                                                                          >  3:19907/1‑143 (MQ=255)
                                                                                                                                                                                                                  gTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTCTAAAACAGGTCATAAACAACCACGATTTCGTTATTCAGTTTTGACCCCTGGCGTTGTGTGGCCAACAACATCTATTAGGGTTGTTTTTCCATCNTCTGGNNCCTTTACCCCCGTatttttggc                                      >  3:17216/1‑267 (MQ=255)
                                                                                                                                                                                                                      cGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTca                                                                                                                                                                      <  1:19907/143‑1 (MQ=255)
                                                                                                                                                                                                                       ggTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATtata                                                                                                                                                               <  7:875968/149‑1 (MQ=255)
                                                                                                                                                                                                                        gTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATaa                                                                                                                                                              >  8:5272/1‑149 (MQ=255)
                                                                                                                                                                                                                        gTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTAtt                                                                                                                              <  1:113714/181‑1 (MQ=255)
                                                                                                                                                                                                                         tGCACTTTCTTACATAAAGGTATTTCCTTTTTTGCGTAAAAGGAGTCCGGGAATTGCCCGGTTTTTCTTACACGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTCAGTTCTGGCCCGCTGCGTGGCGTGGGCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGACACGTgcg                               <  4:613720/275‑1 (MQ=255)
                                                                                                                                                                                                                          gCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATaaaa                                                                                                                                                            <  8:130897/149‑1 (MQ=255)
                                                                                                                                                                                                                           cACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAAc                                                                                                                                                           >  7:849467/1‑149 (MQ=255)
                                                                                                                                                                                                                              tttCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAgg                                                                                                                                                        <  7:257915/149‑1 (MQ=255)
                                                                                                                                                                                                                               ttCTTACATAAAGGTATTCCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGt                                                                                                                                                       <  8:156296/149‑1 (MQ=255)
                                                                                                                                                                                                                                    aCATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAAc                                                                                                                                                           <  6:831680/140‑1 (MQ=255)
                                                                                                                                                                                                                                    aCATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAAc                                                                                                                                                           >  5:831680/1‑140 (MQ=255)
                                                                                                                                                                                                                                     cATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATaaa                                                                                                                                                 <  8:849467/149‑1 (MQ=255)
                                                                                                                                                                                                                                        aaaGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAaacaa                                                                                                                                              <  7:419129/149‑1 (MQ=255)
                                                                                                                                                                                                                                         aaGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTCTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAaacaa                                                                                                                                              >  6:275368/1‑148 (MQ=255)
                                                                                                                                                                                                                                          aGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATtata                                                                                                                                                               >  2:784319/1‑130 (MQ=255)
                                                                                                                                                                                                                                          aGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTCAGt                                                                                                                          >  2:369439/1‑167 (MQ=255)
                                                                                                                                                                                                                                           ccgaTTTCCTTTTCCGCAGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCa                                                                                                                                           >  7:134457/4‑149 (MQ=255)
                                                                                                                                                                                                                                              aTTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTca                                                                                                                                                                      <  2:732828/119‑1 (MQ=255)
                                                                                                                                                                                                                                              aTTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTca                                                                                                                                                                      <  1:201337/119‑1 (MQ=255)
                                                                                                                                                                                                                                              aTTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAAc                                                                                                                                                           <  4:784317/130‑1 (MQ=255)
                                                                                                                                                                                                                                              aTTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTCTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACg                                                                                                                                         <  5:275368/148‑1 (MQ=255)
                                                                                                                                                                                                                                               tttCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAAt                                                                                                                                                                          >  2:416442/1‑114 (MQ=255)
                                                                                                                                                                                                                                               tttCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAAt                                                                                                                                                                          >  1:123891/1‑114 (MQ=255)
                                                                                                                                                                                                                                               tttCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCACGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCCCATTATAAAANAGGTCATAAACAACCACGATTTCGTTATTCAGTTCTGGCACGCTGCTTGGCGTGGGCAAATATCTCTGTNCAGGTTCTTTTTCCATTCGTTGTCACCTTTTCTCCAGTAATTGCGNCNCTTTCCCACCCTGCGGTTCTGCCCCGGt         >  3:72089/1‑275 (MQ=255)
                                                                                                                                                                                                                                                 tCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGAttt                                                                                                                                     >  7:8310/1‑149 (MQ=255)
                                                                                                                                                                                                                                                 tCCTTTTCCGCAGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGAttt                                                                                                                                     >  8:258485/1‑149 (MQ=255)
                                                                                                                                                                                                                                                 tCCTTTTCCGCAGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGAtt                                                                                                                                      >  6:321871/1‑148 (MQ=255)
                                                                                                                                                                                                                                                 tCCTTTTCCGCAGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGAtt                                                                                                                                      >  6:883303/1‑148 (MQ=255)
                                                                                                                                                                                                                                                   cTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTca                                                                                                                                                                      <  3:123890/114‑1 (MQ=255)
                                                                                                                                                                                                                                                   cTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAAc                                                                                                                                                           <  8:708995/125‑1 (MQ=255)
                                                                                                                                                                                                                                                   cTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAAc                                                                                                                                                           >  7:708995/1‑125 (MQ=255)
                                                                                                                                                                                                                                                      ttCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAAt                                                                                                                                                                          >  2:613979/1‑107 (MQ=255)
                                                                                                                                                                                                                                                      ttCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTCAGTTCTGGCCCGCTGCGTGGCGTGGGCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCAt  <  2:611041/275‑1 (MQ=255)
                                                                                                                                                                                                                                                         tGCGGAAAAGGAAATCGTGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTCAGTTCTGGCCCGCTGCGTGGCGTGGGCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTAc                                                     <  4:495439/221‑1 (MQ=255)
                                                                                                                                                                                                                                                         tGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTc                                                                                                                             >  8:730775/1‑149 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                               
CTATTTCGACGTATTTCCAATAGTTTTAATTAAAGGCAGGTCATTGTTATTCACTCTGAATAGTGAATTATTCACTGTCCGCAGAGTAAGAAATATAACTTAGGTATCTATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTCAGTTCTGGCCCGCTGCGTGGCGTGGGCAAATATCTCTGTTCAGGTTCTTTTTCCACTCGCTGTCACCTTTACCCCAGTAATGGCGGCACGTGCGCAGCATGCGGTTCAGCCACGGTTGAGCAT  >  NC_000913/314006‑314526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: