Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,101,541 | G→C | 9.7% | P28R (CCG→CGG) | yggN ← | DUF2884 family putative periplasmic protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,101,541 | 0 | G | C | 9.7% | 537.1 / 42.7 | 165 | P28R (CCG→CGG) | yggN | DUF2884 family putative periplasmic protein |
Reads supporting (aligned to +/- strand): ref base G (76/71); new base C (6/10); total (83/82) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.06e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.42e-02 |
Only 100 of 177 total aligned reads displayed.
CTGCGCATTTTGCTGCTTTCGCCCATCTCCTGAACGATAATTTTATCCAGAGCAATACGGGCTTTCTCGACGCGGCTTTTCGCGCCTTCATCAATCCACGGCAGCGTGCTGCGTAGTTCAGCCTGATAATCCTTCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGTATGCTTTCTTAGTC > NC_000913/3101271‑3101812 | aaTTTTGGTGCTTTCGCCCAACTCCTGAACGATAAGTTGAGACAGAGCAAAACGGGCTTTCTAGACGCGGCTGATCGCGCCTTCATGAAGCGGCGTCAGCGTGCTTCGTAGTTCAGCCTGATGCTACTTGGCTTGCTCGGGCTGAGCGGCATTCAGGGAATATGGCTTACCGTTATACATCACGTTGCCGCCTGGCGTGATCACCAGATTGCCGTGTTCGCGCTTCACCGGCTCGGTTTTCGGGCTGACAATCACATCGT‑CACGGGGAGTAACGc < 4:237777/274‑1 (MQ=255) aTTTTGCTGCTTTCGCCCATCTCCTGAACGATAATTTTATCCAGAGCAATACGGGCTTTCTCGACGCGGCTTTTCGCGCCTTCATCAATCCACGGCAGCGTGCTGCGTAGTTCAGCCTGATAATCCTTCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGc < 1:98025/274‑1 (MQ=255) tAATTTTATCCAGAGCAATACGGGCTTTCTCGACGCGGCTTTTCGCGCCTTCATCAATCCACGGCAGCGTGCTGCGTAGTTCAGCCTGATAATCCTTCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCGTACTATCACATCGT‑CATGCGGCGTGACTCTGCACTCGTAGTCGGCTTGACCCGTCAttt > 1:108506/1‑272 (MQ=255) tAATTTTATCCAGAGCAATACGGGCTTTCTCGACGCGGCTTTTCACACCTTCATCAATCCACGGCAGCGTGCTGCGTAGTTCAGCCTGATAATCCTTCGCCTGCTCGCGCTGGGCGGCAGTCATGTAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCTTGCTCACAAGATGGCCGTTATCGCACTTCACCTGCACGCGTTGAGGGCTGACAATAACATCGT‑CACGCTGCGTGCCGCCGCACTCGTATGCCGCGTGAACGGTCATTGcc > 4:321724/1‑275 (MQ=255) gaactttATGCAGAGCAAGTCTGGCTTTGTCGATGTGGTTTTTCGCGCCTTCATCGAGCGACGGCAGCTGTCTGCGAAGTGAGGCCTGATCAGTCGAAGCCACCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCCTTATACATCTCGGTGCCGTCTGGCTGGAGCACCAGATTGCCGTGTTCGCCCTGCACCTGGACTGTTTGCTGGCTGACAAGCACATCGT‑CACGCGGCGTCACGCGGCACTGGTAGTCGGCGTGATCGGTCACTGcc < 4:681278/271‑1 (MQ=255) ttgcgcgCCTTCTCCAATCCACGGCAGCGTGGTGCGTAGTTCAGCCTGATAATCCTCCGCCTGCTCGCGCTGGCCGGCATTCAGTGAACATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGcatca < 4:167831/272‑1 (MQ=255) ggCAGCGTGCTGCGTAGTTCAGCCTGATAATCCTTCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAAt > 2:679808/1‑275 (MQ=255) ctgcGTAGTTCAGCCTGATAATCCTTCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACgcggcg < 2:238396/166‑1 (MQ=255) ctgcGTAGTTCAGCCTGATAATCCTTCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATA‑‑‑CACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTc < 2:644655/182‑1 (MQ=255) ttCAGCCTGATAATCCTTCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTg > 2:74014/1‑275 (MQ=255) ctgttatgccTGCGCCTGCTCGGGCTGGGCGGCATTAAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTGCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGcccc < 2:704459/266‑1 (MQ=255) tAATCCTTCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGCTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGc > 2:582229/1‑154 (MQ=255) tAATCCTTCGCCTGCCCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAt < 1:105394/274‑1 (MQ=255) aaTCCTTCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCCGCGTg > 8:801290/1‑149 (MQ=255) ccTTCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGTCGTGATCACCAGATTGCCGCTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCa < 4:582227/154‑1 (MQ=255) ttCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCTGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCCGCGTGACga < 6:606897/148‑2 (MQ=255) ttCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCCGCGTGACgat < 7:801290/149‑3 (MQ=255) ttCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCCGCGTGACgat < 7:40274/149‑3 (MQ=255) ttCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCCGCGTGACga < 5:310428/148‑2 (MQ=255) ttCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCCGCGTGACga < 6:735487/148‑2 (MQ=255) tCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTg > 8:264024/1‑149 (MQ=255) cGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCCGCGTGACGATg < 5:170489/148‑1 (MQ=255) cTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAGTCACATCGT‑CACGAGGCGTGCCGCTGCCCTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGACAGTGCTGCCGCCAGCAGACTTTTGCGCATCCTAGTCTTCCCTCAAGAAAAACTCAGGCCAGCATTTGCTGCCCCCACATTGATTACAAAGTAcc > 4:670803/1‑274 (MQ=255) cTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCAAGATTGATAAAAAAGTAcg > 1:154125/1‑275 (MQ=255) cTCGCGCTGGGCGCCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACCGGt < 8:572472/149‑1 (MQ=255) gcgcTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGt > 8:208539/1‑149 (MQ=255) tGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCgg < 6:536797/148‑1 (MQ=255) aTTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCgg < 8:159921/149‑1 (MQ=255) ttCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCCGCGTGACga > 6:786286/1‑123 (MQ=255) gAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCAc > 6:173433/1‑123 (MQ=255) ttGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCa < 7:59257/149‑1 (MQ=255) tGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCa < 5:240565/148‑1 (MQ=255) cTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACAGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAaca > 2:138587/1‑274 (MQ=255) aCCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGaaa < 7:136818/149‑1 (MQ=255) aCCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGaa < 5:568327/148‑1 (MQ=255) gTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGtg < 7:852292/149‑1 (MQ=255) ttATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGtgc < 7:679328/149‑1 (MQ=255) tataCATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCGTCACCGGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATccc < 2:642602/275‑1 (MQ=255) cACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCTCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACAACGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCCCTGAAAGTGCTGCCGCATGCAGCATTTTGCGCATCATAGTCTTCCCCCAAGAAAAAATCAGGCAAGCATTTGCTGTCCACAGATTTATAACCACGTACCCGTTTTCCAGTCCAGACTCGGCGTAACCACGTTATCCCCCCTACt > 4:631292/1‑275 (MQ=255) cACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTTCGGGCTGCCAATCACATCGT‑CTCGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCa > 2:523204/1‑199 (MQ=255) cACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCCCATCGT‑CACGCGGCGTGACGCTGCCCTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAACTGCTGCCGCCAGCACCATTTTGCGCATCATCGTTCTCCCTCCAGACAAAATCAGGCCAACACTTGCTCGCCCCAGCTTGATAAATCACTACGCCGTTTACCTCCCGGGAGCGTCGTTAAACCCTTATCCCAccccat > 4:365343/1‑271 (MQ=255) cACGTTGCCGTCTGGCCTGATCACCAGCTTTCCGTTTTCGCCCTTCACCTGCCCGGTTTGCGGTCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGGGCGCGGTCATTACCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATCGTCTTCCCCCAAGAAAAAATCAGGCCAGCAGTTGCTGGCCCCAGATTGCTAACACAGTCCGCGTTTTCCATGCCGGATCCGGCGTACACACGTTCTCCCCCCTAcc > 4:351445/1‑274 (MQ=255) aCGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCcag > 7:320522/1‑149 (MQ=255) cGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTc > 2:356792/1‑274 (MQ=255) cGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAGGCGGCGTAAACACCTTATCCCGCCTACTc > 1:185427/1‑274 (MQ=255) cGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGGGCTGCCGCCAGCAGCATTTTGCGACTCATAGTCTTCCCTCAAAAAAAAATCAGGCCAGCATTTGCTGGCCCCAGCTTGATAAAAAATTACGCGTTTTCCAGGCCGGNNNGGGCGTCAAACCCTTATccccactccac > 3:214066/1‑266 (MQ=255) gTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACTCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCCTAGTCTTCCCTCAAGCAAAAATCAGGCCAGCATTTCCTTGCCCCAGCTTGCTAAAAACGTAACACTTTTACATCACGGTTCCTGCGAAAACACCTTCTCCCCCCtcccct > 4:252362/1‑269 (MQ=255) ttGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACgcggcg < 2:258462/85‑1 (MQ=255) ttGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCcagca > 6:746141/1‑148 (MQ=255) ttGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCcagca > 5:495459/1‑148 (MQ=255) tGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCg > 2:800353/1‑107 (MQ=255) gCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATccc < 1:214067/262‑1 (MQ=255) gCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGC‑CACGCGGCGTGACGCTGCACTGGTAATCGGCGTGAGCGGTCATTGCAGTCACTGAAAGTGCTGCCGCCAgcagca > 7:566444/1‑149 (MQ=255) ccGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCAt > 7:583415/1‑149 (MQ=255) gTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAg < 4:800351/107‑1 (MQ=255) catcgcAGGTTGCGGTTTTCGCCCTTCCCCTGCCCGGTTGACGGGGTGAAAATCAC‑CCGTCCACGCGGCTGGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCCCTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATCGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCa < 4:614318/269‑1 (MQ=255) accaagttGCCGTTTCAGCCCTTCCCCTGCACGGTTTGCGGGCTGACAATCACATCGT‑AACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGc < 4:356790/268‑1 (MQ=255) ccAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTg > 5:807987/1‑79 (MQ=255) ccAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGt > 6:511916/1‑148 (MQ=255) ccAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGt > 6:812807/1‑148 (MQ=255) aGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCt < 5:766444/148‑1 (MQ=255) aTTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTcc < 7:631057/149‑1 (MQ=255) tGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAg > 2:298345/1‑275 (MQ=255) gCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCt < 6:180740/148‑1 (MQ=255) gCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGaaa > 7:53501/1‑98 (MQ=255) gCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAATCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGaaaaaa < 8:566444/149‑1 (MQ=255) cccTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGaaaaaa < 5:594237/148‑1 (MQ=255) cTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGaaaa > 6:589780/1‑144 (MQ=255) cTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCa < 8:392403/149‑1 (MQ=255) ttCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCa < 5:747258/148‑1 (MQ=255) ttCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCa > 5:53686/1‑148 (MQ=255) gCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGc < 6:910600/148‑1 (MQ=255) tttGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCAttt > 4:597773/1‑275 (MQ=255) aagggCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGc < 7:776227/147‑1 (MQ=255) cGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTgcg < 4:289005/102‑1 (MQ=255) gggCTTACAATCACATCGT‑CGCGCGGCGTGACGGTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTAACTGAAAGTGCTGCTGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCAt < 2:321726/275‑1 (MQ=255) ggCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGccc > 6:880814/1‑148 (MQ=255) tGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAg > 5:714535/1‑148 (MQ=255) gACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACTCGTTTTCCATGCCGCATGCCGCGTCAACACCTTATCCCGCCTACTCGATCGTGCATCTCTCAAGGCCTTATAAGCTTTGCACCTCAGTCAGTTTTTCATTTGTCATCcccc > 4:250106/1‑275 (MQ=255) aCAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAtt > 8:832397/1‑149 (MQ=255) aCAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAt < 5:511916/148‑1 (MQ=255) aaTCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGa < 7:356914/149‑1 (MQ=255) tCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGTCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATa > 7:73111/1‑149 (MQ=255) cacaTCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATa > 6:403999/1‑148 (MQ=255) acaTCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAAc > 8:542172/1‑149 (MQ=255) agcGT‑CACGCCGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaa > 8:365270/3‑149 (MQ=255) tCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGa < 8:636777/142‑1 (MQ=255) tCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaaa < 8:507871/149‑1 (MQ=255) tCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaa < 6:495459/148‑1 (MQ=255) cGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaaa < 5:880814/148‑1 (MQ=255) cGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAg < 8:320522/149‑1 (MQ=255) cGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCCTTTTTCATCGCCCg > 2:615849/1‑265 (MQ=255) cGT‑CACGCCGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCCCAAGAAAAAATCAGGCCAGCATTTGCTg > 7:340615/1‑129 (MQ=255) cGT‑CACGCCGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCCCAAGAAAAAATCAGGCCAGCATTTGCTg < 8:340615/129‑1 (MQ=255) gT‑CACGCCGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGt < 6:51333/51‑1 (MQ=255) aCGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCgg > 2:507071/1‑164 (MQ=255) cgcgGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaa > 5:532039/1‑142 (MQ=255) cgcgGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCg > 4:70325/1‑187 (MQ=255) cgcgGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGTATGCTTTCTTAGTc < 2:276066/275‑1 (MQ=255) ggcgTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgc < 4:507069/164‑1 (MQ=255) | CTGCGCATTTTGCTGCTTTCGCCCATCTCCTGAACGATAATTTTATCCAGAGCAATACGGGCTTTCTCGACGCGGCTTTTCGCGCCTTCATCAATCCACGGCAGCGTGCTGCGTAGTTCAGCCTGATAATCCTTCGCCTGCTCGCGCTGGGCGGCATTCAGGGAATATTGCTTACCGTTATACATCACGTTGCCGTCTGGCGTGATCACCAGATTGCCGTTTTCGCCCTTCACCTGCACGGTTTGCGGGCTGACAATCACATCGT‑CACGCGGCGTGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGTATGCTTTCTTAGTC > NC_000913/3101271‑3101812 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |