Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	481,228	C→A	10.4%	intergenic (‑520/+26)	tomB ← / ← acrB	Hha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	481,228	0	C	A	10.4%	290.7 / 20.1	88	intergenic (‑520/+26)	tomB/acrB	Hha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (42/36);  new base A (5/4);  total (47/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.00e-04

TCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTAAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGT‑CATCAGGATCGGACGTAAACGCAT  >  NC_000913/480991‑481496                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                              tctGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCGAAATGATTAAGGATTATTAAATCTATTAACACTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATc                                                                                                                                                                                                                                          <  2:181239/275‑1 (MQ=255)                                                              ccTCTAAATGATTATGGATTATTAAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGAt                                                                                                                                                                                                                                                                 >  1:182332/1‑191 (MQ=255)                                                                  tAAATGATTATGGATTATTAAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTaca                                                                                                                                                                                                                                                             <  3:182331/191‑1 (MQ=255)                                                                                             tAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTAcgc                                                                                                                                                                                                                                                                             >  6:739037/1‑148 (MQ=255)                                                                                             tAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTAcgc                                                                                                                                                                                                                                                                             <  5:739037/148‑1 (MQ=255)                                                                                                    aCTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCGTAAGCGGCCttt                                                                                                                                                                                                                                                                      <  5:814331/148‑2 (MQ=255)                                                                                                               aTTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTa                                                                                                                                                                                                                                                      >  4:816225/1‑153 (MQ=255)                                                                                                                  aactaacAATCAGTATTTTTTATGAATTTTATTCGTTGTTAATACTGGTTTTCGGATGAGATACTGATTTGGTGGTTCAATTACTCCTTAATGTTCGTATGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAGAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATccc                                                                                                                           <  1:211964/273‑1 (MQ=255)                                                                                                                   actaacAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCaa                                                                                                                                                                                                                                                  <  2:816227/153‑1 (MQ=255)                                                                                                                    ctaacAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCt                                                                                                                                                                                                                              >  2:596483/1‑172 (MQ=255)                                                                                                                     taacAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATc                                                                                                                                                                                                                                                    >  7:542964/1‑149 (MQ=255)                                                                                                                        cAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGc                                                                                                                                                                                                                          <  4:596481/172‑1 (MQ=255)                                                                                                                             aGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGAt                                                                                                                                                                                                                                                                 >  2:796461/1‑128 (MQ=255)                                                                                                                                 ttttttATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTaca                                                                                                                                                                                                                                                             <  4:796459/128‑1 (MQ=255)                                                                                                                                       aTGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTac                                                                                                                                                                                                                                                              >  4:809932/1‑121 (MQ=255)                                                                                                                                         gAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATgg                                                                                                                                                                                                                                <  8:542964/149‑1 (MQ=255)                                                                                                                                           aTTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGt                                                                                                                                                                                                                                                          <  2:809934/121‑1 (MQ=255)                                                                                                                                           aTTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGc                                                                                                                                                                                                                               <  6:633892/148‑1 (MQ=255)                                                                                                                                                 tCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTc                                                                                                                                                                                                                        >  8:410307/1‑149 (MQ=255)                                                                                                                                                    gTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGcc                                                                                       >  1:142576/1‑275 (MQ=255)                                                                                                                                                      ggTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGcc                                                                                                                                                                                                                                                                         >  4:91352/1‑95 (MQ=255)                                                                                                                                                      ggTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAatgat                                                                                                                                                                                                                                              >  1:194461/1‑122 (MQ=255)                                                                                                                                                      ggTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCtgtg                                                                                                                                                                                                                           >  4:717195/1‑141 (MQ=255)                                                                                                                                                          aaTACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAg                                                                                                                                                                                                                                                                     <  2:91353/95‑1 (MQ=255)                                                                                                                                                          aaTACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATc                                                                                                                                                                                                                                          <  3:194460/122‑1 (MQ=255)                                                                                                                                                          aaTACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCg                                                                                                                                                                                                                       <  2:717197/141‑1 (MQ=255)                                                                                                                                                               tGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACgaa                                                                                                                                                            >  4:60386/1‑195 (MQ=255)                                                                                                                                                                gtttttCTTTTGGGTTCCTGATTTTGTGGTTCAATTATCCCTTAATGTTCGTAGGTTATGCCTAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCAGTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCAc                                                                            <  4:654015/272‑1 (MQ=255)                                                                                                                                                                 gTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAatga                                                                                                                                                                                                                                               >  4:148507/1‑110 (MQ=255)                                                                                                                                                                 gTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAaccac                                                                                                                                                                                 >  2:610472/1‑172 (MQ=255)                                                                                                                                                                 gTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCAcc                                                                           >  1:103774/1‑274 (MQ=255)                                                                                                                                                                 gTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCAcc                                                                           >  2:654017/1‑274 (MQ=255)                                                                                                                                                                   tttCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGaagaag                                                                                                                                                        <  2:60387/195‑1 (MQ=255)                                                                                                                                                                     tCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTAcgc                                                                                                                                                                                                                                                                             <  4:77292/76‑1 (MQ=255)                                                                                                                                                                     tCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATgatgat                                                                                                                                                                                                                                           <  2:148508/110‑1 (MQ=255)                                                                                                                                                                     tCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACcaca                                                                                                                                                                             <  4:610470/172‑1 (MQ=255)                                                                                                                                                                      cGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGc                                                                                                                                                                                                                          >  2:809940/1‑126 (MQ=255)                                                                                                                                                                      cGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCAt                                                                                                                                                                                                             >  2:737283/1‑139 (MQ=255)                                                                                                                                                                      cGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACgaa                                                                                                                                                            >  2:626212/1‑188 (MQ=255)                                                                                                                                                                          tGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGa                                                                                                                                                                                                                      <  4:809938/126‑1 (MQ=255)                                                                                                                                                                          tGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCtt                                                                                                                                                                                                         <  4:737281/139‑1 (MQ=255)                                                                                                                                                                          tGAGATCCTGAGTTGGTGGTTAAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGTCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGaagaag                                                                                                                                                        <  4:626210/188‑1 (MQ=255)                                                                                                                                                                              aTCCTGAGTTGTTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTaa                                                                                                                                                                                                 >  7:782323/1‑143 (MQ=255)                                                                                                                                                                              aTCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTaa                                                                                                                                                                                                 <  8:782323/143‑1 (MQ=255)                                                                                                                                                                              aTCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcgg                                                                                                                                                                                            >  5:893902/1‑148 (MQ=255)                                                                                                                                                                                  tGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggcg                                                                                                                                                                                       <  7:410307/149‑1 (MQ=255)                                                                                                                                                                                      ttGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCCCCATCCCGCCCATTACACCGGTACCTACTGCCTTCTGCGCCCCCGAACCCGCCCCCGTCCTGATAACCCGCGCCAt                                                      >  4:553796/1‑274 (MQ=255)                                                                                                                                                                                         gtggtTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCt                                                                                                                                                                                                   >  2:128187/1‑130 (MQ=255)                                                                                                                                                                                           ggtTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGcc                                                                                                                                                  >  4:55996/1‑177 (MQ=255)                                                                                                                                                                                            gtTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACcaca                                                                                                                                                                             >  7:82465/1‑149 (MQ=255)                                                                                                                                                                                             ttCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAg                                                                                                                                                                                               <  4:128186/130‑1 (MQ=255)                                                                                                                                                                                              tCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACgaa                                                                                                                                                            >  2:811682/1‑164 (MQ=255)                                                                                                                                                                                               cAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTa                                                                                                                                              <  2:55997/177‑1 (MQ=255)                                                                                                                                                                                                  ttACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGaagaag                                                                                                                                                        <  4:811680/164‑1 (MQ=255)                                                                                                                                                                                                          tAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTAcgcg                                                                                                                                                                                                                                                                            >  2:459688/1‑40 (MQ=255)                                                                                                                                                                                                          tAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggc                                                                                                                                                                                           >  4:742589/1‑121 (MQ=255)                                                                                                                                                                                                            ttGTTCTTGGGTTATGAATAAAAACGGGCNGTTTGGCATTATTTGTGATTATTAANTGTATAAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCTGCTAAAGCGGCGGCGAACCACCACAAAGNATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCg                               <  3:142575/274‑1 (MQ=255)                                                                                                                                                                                                              gTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCt                                                                                                                                                                                                                                                                        <  4:459686/40‑1 (MQ=255)                                                                                                                                                                                                              gTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggcg                                                                                                                                                                                       <  2:742591/121‑1 (MQ=255)                                                                                                                                                                                                                tCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGt                           >  2:474478/1‑275 (MQ=255)                                                                                                                                                                                                                  gTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAAtgatga                                                                                                                                                                                                                                            >  1:40913/1‑64 (MQ=255)                                                                                                                                                                                                                      gTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGa                                                                                                                                                                                                                                        <  3:40912/64‑1 (MQ=255)                                                                                                                                                                                                                         aTGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTCAAGTGGCGGCGAACCAACACAACGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCCCCCATTACCCCGGTACCTACTCCGTTCTGCCCGCCGGAACCACCCCCAGTTATGATAACCACCCGCCTACCCCCCAAGAAAAACCCCAACAGGGTCCATCAGGa                  >  4:115245/1‑275 (MQ=255)                                                                                                                                                                                                                         aTGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggc                                                                                                                                                                                        >  1:189241/1‑109 (MQ=255)                                                                                                                                                                                                                             aTAAAAGAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAaccacc                                                                                                                                                                                >  6:499318/1‑113 (MQ=255)                                                                                                                                                                                                                             aTAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAaccacc                                                                                                                                                                                <  5:499318/113‑1 (MQ=255)                                                                                                                                                                                                                             aTAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAac                                                                                                                                                                                    <  3:189240/109‑1 (MQ=255)                                                                                                                                                                                                                                actaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggc                                                                                                                                                                                        >  8:799966/4‑102 (MQ=255)                                                                                                                                                                                                                                actaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggc                                                                                                                                                                                        <  7:799966/99‑1 (MQ=255)                                                                                                                                                                                                                                 ctaaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTg                                                                                                                                        >  7:39434/3‑149 (MQ=255)                                                                                                                                                                                                                                 aaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGTCGAACCACCACAAAGAATACCGGAACGAAGAAGATCAACAGTACCGTTGCGGTCACCATCCCGCCAATTACACCGGTCTCTACTACCGTCTGCGCGACGGAACCACCACCAGTAATGATAACCAGACGCATATCGCCAAGGATCAACCACTCAGAGGT‑CACCAGGATCggcagg          >  4:393803/1‑270 (MQ=255)                                                                                                                                                                                                                                 aaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGAtt                                                                                                                                                     <  7:683856/136‑1 (MQ=255)                                                                                                                                                                                                                                 aaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGAtt                                                                                                                                                     >  8:683856/1‑136 (MQ=255)                                                                                                                                                                                                                                 aaaaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGtt                                                                                                                                         >  6:176670/1‑148 (MQ=255)                                                                                                                                                                                                                                  taaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcgg                                                                                                                                                                                            <  7:13008/95‑1 (MQ=255)                                                                                                                                                                                                                                  taaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcgg                                                                                                                                                                                            >  8:13008/2‑96 (MQ=255)                                                                                                                                                                                                                                  taaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGaa                                                                                                                                                                        <  7:435498/115‑1 (MQ=255)                                                                                                                                                                                                                                  taaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGaa                                                                                                                                                                        >  8:435498/2‑116 (MQ=255)                                                                                                                                                                                                                                  taaGGCCGCGTAAGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGc                                                                                                                                       >  7:178614/2‑149 (MQ=255)                                                                                                                                                                                                                                   aaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAAt                                                                                                                                                                       >  6:364903/1‑116 (MQ=255)                                                                                                                                                                                                                                   aaGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAAt                                                                                                                                                                       <  5:364903/116‑1 (MQ=255)                                                                                                                                                                                                                                      gCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTaaa                                                                                                                                                                                                >  2:426262/1‑88 (MQ=255)                                                                                                                                                                                                                                      gCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACgaag                                                                                                                                                           >  1:102529/1‑125 (MQ=255)                                                                                                                                                                                                                                        cggttACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGT‑CATCAGGATCGGACGTAAACGCAt  <  2:553798/272‑1 (MQ=255)                                                                                                                                                                                                                                         gCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcggcggc                                                                                                                                                                                        >  3:128755/1‑93 (MQ=255)                                                                                                                                                                                                                                          cTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAgcgg                                                                                                                                                                                            <  4:426260/88‑1 (MQ=255)                                                                                                                                                                                                                                          cTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACgaa                                                                                                                                                            >  7:876549/1‑120 (MQ=255)                                                                                                                                                                                                                                          cTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACgaa                                                                                                                                                            <  8:876549/120‑1 (MQ=255)                                                                                                                                                                                                                                          cTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAagaaga                                                                                                                                                       <  3:102528/125‑1 (MQ=255)                                                                                                                                                                                                                                          cTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCATTGCGGTCACCa                                                                                                                               >  8:319232/1‑149 (MQ=255)                                                                                                                                                                                                                                           ttACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCAt                                                                                                                              >  8:487573/1‑149 (MQ=255)                                                                                                                                                                                                                                             aCGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAac                                                                                                                                                                                    <  1:128756/93‑1 (MQ=255)                                                                                                                                                                                                                                              cgcgGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATccc                                                                                                                           <  8:82465/149‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                              TCTGAATATGACGTAATAACCGAGGAATGAATAAAGAATTACCGCAAATAATTAAGAATAGCCTCTAAATGATTATGGATTATTAAATCTATTAGCGCTACTTAATATAAATTAACTAACAATCAGTATTTTTTATGAATTTTATCCGTGGTTAATACTGGTTTTCGTATGAGATCCTGAGTTGGTGGTTCAATTACTCCTTAATGTTCGTAGGTTATGCATAAAAAAGGCCGCTTACGCGGCCTTAGTGATTACACGTTGTATCAATGATGATCGACAGTATGGCTGTGCTCGATATCTTCATTCTTGCGGCTAAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGT‑CATCAGGATCGGACGTAAACGCAT  >  NC_000913/480991‑481496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑