BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	657,535	C→A	6.8%	intergenic (+34/+20)	cspE → / ← flc	constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	657,535	0	C	A	6.8%	558.6 / 23.5	161	intergenic (+34/+20)	cspE/flc	constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein
Reads supporting (aligned to +/- strand): ref base C (83/67); new base A (6/5); total (89/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.78e-02

Only 100 of 162 total aligned reads displayed.

TCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAA‑ATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTC  >  NC_000913/657273‑657793

tCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttt                                                                                                                                                                                                                                                          <  2:70694/275‑1 (MQ=255)

                                aaGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtt                                                                                                                                                                                                                                                              >  4:155499/1‑239 (MQ=255)

                                    tAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttt                                                                                                                                                                                                                                                          <  2:155500/239‑1 (MQ=255)

                                            aGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCAGAAAACAGCCAGAATGcc                                                                                                                                                                                                              >  2:79209/1‑275 (MQ=255)

                                                           ccAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGCCTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGACTGCCA‑GGTAGGTCATTGCa                                                                                                                                                                                              >  2:650120/1‑275 (MQ=255)

                                                              aaGGATTCTGTTTCATTACTCCGGGAGCAGGCAGCTAAGACGTGTTCGTACCCTTTACTGCAATCCAGACTAATTGTTTTAAAACTCTTGCTGAATGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCaaaa                                                                                                                                                                                           <  4:79208/275‑1 (MQ=255)

                                                                           cATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAACACAGCCAGAATGCAA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGt                                                                                                                                                                              >  2:398425/1‑275 (MQ=255)

                                                                             ttACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGATGAAGGTCAGCGCGTAGAGTTCGAACTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTACTCGCTCTGTAAGATACGTCAGCAAGAATTCAACACCCGCTTAATCAGCGGGTTTTTTTTGGTATTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGACA‑GTGCGGTCATGCAAACAGACCCCAGAAGGTTg                                                                                                                                                                            >  1:13926/1‑275 (MQ=255)

                                                                                           ggCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCg                                                                                                                                                                                                       >  2:496585/1‑234 (MQ=255)

                                                                                                          ttCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGccggtatctt                                                                                                                                               >  2:20526/1‑267 (MQ=255)

                                                                                                              gacacTTCTATGCAACCCAGACTAAGTGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACg                                                                                                                                                                   <  4:20526/250‑1 (MQ=255)

                                                                                                                   ttCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCa                                                                                                                                                                                                                                                                     >  8:736958/1‑149 (MQ=255)

                                                                                                                     ctctGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGGTAAGTAATCCCCAGCCACAGAGGccccatt                                                                                                                                    >  4:563749/1‑271 (MQ=255)

                                                                                                                      tctGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCg                                                                                                                                                                                                                                                                  <  7:447330/149‑1 (MQ=255)

                                                                                                                       ctGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCg                                                                                                                                                                                                                                                                  <  5:571294/148‑1 (MQ=255)

                                                                                                                        tGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGc                                                                                                                                                                                                               >  4:450859/1‑198 (MQ=255)

                                                                                                                         gCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGt                                                                                                                                                                                                                                                               >  7:903769/1‑149 (MQ=255)

                                                                                                                            aTCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑Gt                                                                                                                                                                                                          <  2:450861/198‑1 (MQ=255)

                                                                                                                            aTCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttt                                                                                                                                                                                                                                                             <  5:453461/148‑1 (MQ=255)

                                                                                                                             tCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaaa                                                                                                                            <  2:491070/275‑1 (MQ=255)

                                                                                                                              ccAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGt                                                                                                                                                                                                                                                               <  5:754999/144‑1 (MQ=255)

                                                                                                                              ccAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGt                                                                                                                                                                                                                                                               >  6:754999/1‑144 (MQ=255)

                                                                                                                               cAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttt                                                                                                                                                                                                                                                          <  5:217510/148‑1 (MQ=255)

                                                                                                                                aGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttttt                                                                                                                                                                                                                                                        <  7:736958/149‑1 (MQ=255)

                                                                                                                                 gACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttttt                                                                                                                                                                                                                                                        >  6:223111/1‑148 (MQ=255)

                                                                                                                                 gACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTg                                                                                                                                                                                                                                                       >  7:789804/1‑149 (MQ=255)

                                                                                                                                  aCTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTgg                                                                                                                                                                                                                                                      >  1:42255/1‑149 (MQ=255)

                                                                                                                                  aCTAATGGCTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTCACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGACTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACCGCCAGAACGCCA‑GTGCGGCCATGGCAAAAGACCCCAGAAGGCTGACGCAAACGTCCAGGAATGCCCAGCCAAAGCGCCCCACTTGTAACAAAAacac                                                                                                                       >  4:99497/1‑275 (MQ=255)

                                                                                                                                      aTGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGt                                                                                                                                                                  <  4:605946/228‑1 (MQ=255)

                                                                                                                                          ttttAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAg                                                                                                                                                                                                                                               >  6:191094/1‑148 (MQ=255)

                                                                                                                                              aaaaCTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAATCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGCGTGc                                                                                                                                                                                                                                          >  8:564088/1‑149 (MQ=255)

                                                                                                                                                aaCTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCgg                                                                                                                                                                                                                                        >  7:71901/1‑149 (MQ=255)

                                                                                                                                                aaCTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCgg                                                                                                                                                                                                                                        >  7:489775/1‑149 (MQ=255)

                                                                                                                                                  ctctTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGt                                                                                                                                                                                                                                       <  5:514529/148‑1 (MQ=255)

                                                                                                                                                   tctTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTg                                                                                                                                                                                                                                     >  7:518050/1‑149 (MQ=255)

                                                                                                                                                    ctTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGaaaa                                                                                                                                                                     >  4:651754/1‑211 (MQ=255)

                                                                                                                                                     ttGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGc                                                                                                                                                                                                                                                                   >  7:140890/1‑117 (MQ=255)

                                                                                                                                                       gCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGc                                                                                                                                                                                                                                 >  7:33645/1‑149 (MQ=255)

                                                                                                                                                        cTGAAGGTCAGCGCGTAGAGTTCGAACTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGGAAGATACGTCAGCAAGAATTCAAAACCCGCGTAAGCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACAAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTgag                                                                                                 <  2:99498/275‑1 (MQ=255)

                                                                                                                                                         tGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCATCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCg                                                                                                                                                                                                                               <  7:564088/149‑1 (MQ=255)

                                                                                                                                                          gAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTTCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAACACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCGTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGaa                                                                                               <  1:107407/275‑1 (MQ=255)

                                                                                                                                                                  gcgcGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGCGGCCGAAAACAGcc                                                                                                                                                                                                                      <  7:67233/149‑1 (MQ=255)

                                                                                                                                                                  gcgcGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGcc                                                                                                                                                                                                                      >  7:703728/1‑149 (MQ=255)

                                                                                                                                                                  gcgcGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGcc                                                                                                                                                                                                                      <  8:789804/149‑1 (MQ=255)

                                                                                                                                                                   cgcgTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAc                                                                                                                                                                                                                          >  6:576401/1‑144 (MQ=255)

                                                                                                                                                                       tAGAGTTCGAAATCATTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCg                                                                                                                                                                                                       <  2:793486/158‑1 (MQ=255)

                                                                                                                                                                         gagTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATg                                                                                                                                                                                                                >  6:786799/1‑148 (MQ=255)

                                                                                                                                                                                      cTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGATTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTTGTCTTTAGTGTGCGATTGAGGCCGAAAACAGCCAGAATGCGA‑GTGCGGTCATTGCAAAAGACCCCAGAATGTTGAAGAACACGTTCAGTAATGCCAATCCAAAGCATCTCCCTTTTAACAAAAACACCACTTTTGCGCAACATGTTGAGACGGTTGTTAGACCGCCACACC‑ATCCgg                                                                    >  4:355039/1‑273 (MQ=255)

                                                                                                                                                                                       tAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATgg                                                                                                                                                                                                <  8:545418/149‑1 (MQ=255)

                                                                                                                                                                                             tGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGGCTTTAGGGTTCGGGTGGGGCCGAAAACAGCCAGAATGCCAGGTCGGGTCATGG‑AAAAAACCCCAGAAGGTTGACGGAAAAGGTTAGGAATGCCCAAcc                                                                                                                                                  >  2:181162/1‑189 (MQ=255)

                                                                                                                                                                                               ccAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCctc                                                                                                                                      >  4:578212/1‑199 (MQ=255)

                                                                                                                                                                                                  aaGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAg                                                                                                                                                                                                                                               <  8:432001/92‑1 (MQ=255)

                                                                                                                                                                                                   aGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGt                                                                                                                                  <  2:578214/199‑1 (MQ=255)

                                                                                                                                                                                                      cccTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGa                                                                                                                                                                                  <  6:207809/148‑1 (MQ=255)

                                                                                                                                                                                                        cTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAg                                                                                                                                                                                <  5:786799/148‑1 (MQ=255)

                                                                                                                                                                                                          tCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGtt                                                                                                                                                                             <  8:166554/149‑1 (MQ=255)

                                                                                                                                                                                                            tgctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGa                                                                                                                                                                           <  8:301396/149‑1 (MQ=255)

                                                                                                                                                                                                                   aaCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGaaaa                                                                                                                                                                     <  5:191094/148‑1 (MQ=255)

                                                                                                                                                                                                                     cGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACg                                                                                                                                                                   <  6:373812/148‑1 (MQ=255)

                                                                                                                                                                                                                      gTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGtt                                                                                                                                                                 <  7:24341/149‑1 (MQ=255)

                                                                                                                                                                                                                      gTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGt                                                                                                                                                                  <  5:674593/148‑1 (MQ=255)

                                                                                                                                                                                                                        aaTCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCCAAAACAGCCAGAATGCCA‑GTTCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAACGCGGCCCTCTTCTAACAACAACACACCTTCTTCCGAAAACGTTGAGAAGGTTGTTAGACCGCCACAAC‑ACCCGGTGGTGATTAATACCTTCCCACCTGGATCAATCTTc                                 >  4:307420/1‑274 (MQ=255)

                                                                                                                                                                                                                         aTCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCa                                                                                                                                                               >  5:879470/1‑148 (MQ=255)

                                                                                                                                                                                                                           cGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGaaa                                                                                                                                                                      >  6:45919/1‑139 (MQ=255)

                                                                                                                                                                                                                           cGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGaaa                                                                                                                                                                      <  5:45919/139‑1 (MQ=255)

                                                                                                                                                                                                                                tGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGc                                                                                                                                                        <  6:191521/148‑1 (MQ=255)

                                                                                                                                                                                                                                   aaGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAg                                                                                                                                                    >  8:772631/1‑149 (MQ=255)

                                                                                                                                                                                                                                     gATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGc                                                                                                                                                   >  5:250990/1‑148 (MQ=255)

                                                                                                                                                                                                                                          gTCAGCAAGAATTCAACACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGACCGAAAACAGCCAGAATGCTA‑GTCCGGCCATTGCAAAAGACCCCAGACGGTTGACAAAAACATTCCTTAAGTCCCAGCCAAACCGGCCCCCTCGGAACAAACACACCAATGCCGCCTAACATGTTGAGAAGGCTGTTA‑ACACGCACAAACATCCGCAGGTGATTAATCATTTCCACACGGGAAACAAGTTCGTCATCCGGCTGatacat               >  4:684368/1‑269 (MQ=255)

                                                                                                                                                                                                                                          gTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGaa                                                                                                                                                                       >  1:176208/1‑123 (MQ=255)

                                                                                                                                                                                                                                          gTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGc                                                                                                                                             >  1:151637/1‑149 (MQ=255)

                                                                                                                                                                                                                                          gTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTg                                                                                                   >  4:587821/1‑191 (MQ=255)

                                                                                                                                                                                                                                          gTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAA‑ATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATg              >  2:509489/1‑275 (MQ=255)

                                                                                                                                                                                                                                           tCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCg                                                                                                                                            <  7:772631/149‑1 (MQ=255)

                                                                                                                                                                                                                                            cAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTc                                                                                                                                                                                                                                                    >  2:156423/1‑45 (MQ=255)

                                                                                                                                                                                                                                            cAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTTTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAA‑ATCAGGTGGTGATACATACCTCACACACTGGATCAATGTTCGTCATCCTGCTGAACCGTGCg            >  2:604551/1‑275 (MQ=255)

                                                                                                                                                                                                                                            cAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAA‑ATCCGGTGGTGATTAATACtt                                                     >  2:368923/1‑234 (MQ=255)

                                                                                                                                                                                                                                            cAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAA‑ATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTc                                 >  2:663199/1‑254 (MQ=255)

                                                                                                                                                                                                                                            cAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAA‑ATCCGGTGGGGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCg            >  2:545565/1‑275 (MQ=255)

                                                                                                                                                                                                                                              gCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACg                                                                                                                                                                   <  3:176207/123‑1 (MQ=255)

                                                                                                                                                                                                                                              gCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGcc                                                                                                                                         <  3:151636/149‑1 (MQ=255)

                                                                                                                                                                                                                                              gCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGaa                                                                                               <  2:587823/191‑1 (MQ=255)

                                                                                                                                                                                                                                                aaGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTa                                                                                                                                                                                                                                                <  4:156422/45‑1 (MQ=255)

                                                                                                                                                                                                                                                 aGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCct                                                                                                                                       >  5:134019/1‑148 (MQ=255)

                                                                                                                                                                                                                                                   aaTTCAAAACCCGCTTAATCAGCGGGCTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCctct                                                                                                                                     >  5:463456/1‑148 (MQ=255)

                                                                                                                                                                                                                                                     ttCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAA‑ATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtccta     <  2:307421/273‑1 (MQ=255)

                                                                                                                                                                                                                                                      tCAAACACCGCTTAATCAGCGGGTTTTTTTTGGTCGTTAGTGTGGGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAA‑ATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattc  <  4:667683/275‑1 (MQ=255)

                                                                                                                                                                                                                                                       cAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTa                                                                                                                                 <  6:673364/148‑1 (MQ=255)

                                                                                                                                                                                                                                                        aaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTc                                                                                                                                                                >  6:797186/1‑116 (MQ=255)

                                                                                                                                                                                                                                                          aaCCCGCTTAATCAGCGGGCTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACa                                                                                                                              <  6:463456/148‑1 (MQ=255)

                                                                                                                                                                                                                                                           aCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaaa                                                                                                                            >  8:205716/1‑149 (MQ=255)

                                                                                                                                                                                                                                                           aCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaaa                                                                                                                            >  7:22443/1‑149 (MQ=255)

                                                                                                                                                                                                                                                            cccGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaaa                                                                                                                            >  6:292540/1‑148 (MQ=255)

                                                                                                                                                                                                                                                             ccGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTaa                                                                                                                                <  7:426486/143‑1 (MQ=255)

                                                                                                                                                                                                                                                             ccGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTaa                                                                                                                                >  8:426486/1‑143 (MQ=255)

                                                                                                                                                                                                                                                               gCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATga                                                                                                                                                        <  8:675762/117‑2 (MQ=255)

                                                                                                                                                                                                                                                               gCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAac                                                                                                                         >  6:697816/1‑148 (MQ=255)

                                                                                                                                                                                                                                                                cTTAATCAGCGTGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAaca                                                                                                                        >  6:164283/1‑148 (MQ=255)

                                                                                                                                                                                                                                                                cTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATg                                                                                                                                                         <  5:191511/115‑1 (MQ=255)

                                                                                                                                                                                                                                                                  tAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAAcacc                                                                                                                      >  5:306684/1‑148 (MQ=255)

TCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCA‑GTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAA‑ATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTC  >  NC_000913/657273‑657793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`