BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	672,110	G→A	5.6%	T26I (ACC→ATC)	lptE ←	LPS assembly OM complex LptDE, lipoprotein component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	672,110	0	G	A	5.6%	593.6 / 17.2	178	T26I (ACC→ATC)	lptE	LPS assembly OM complex LptDE, lipoprotein component
Reads supporting (aligned to +/- strand): ref base G (82/86); new base A (4/6); total (86/92)
Fisher's exact test for biased strand distribution p-value = 7.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-02

Only 100 of 180 total aligned reads displayed.

CGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAAC  >  NC_000913/671849‑672368

cGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCCTAGTGGAAGGACCCTGCGTGGTATCCCGCTGAt                                                                                                                                                                                                                                                        >  4:340844/1‑274 (MQ=255)

      gggATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATgccagcc                                                                                                                                                                                                                                                 >  2:432299/1‑275 (MQ=255)

                 ggtagatttAACAGGCATGGGCATCTGTTACTCTGTTGTTTGCCCGTTACGAAATACCGAAGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCGTTATCAAGCAACTGGATACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCcggcg                                                                                                                                                                                                                                       <  4:6933/267‑1 (MQ=255)

                   tCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTg                                                                                                                                                                                                                                    >  1:172873/1‑275 (MQ=255)

                                             tACTCTGCTTTTTTACCGTTACGGAATACCGATGCGGTATCTTTCGCGTTGCTCCCTTTTCCCAAACGCAAGGATGGAACGTCCTTACGCTTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAAcaacaa                                                                                                                                                                                                          <  4:432297/275‑1 (MQ=255)

                                                               atACGGAAAACCGATGCGGTATTTTTCGCGTTGATAACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTGTCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGAACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCAc                                                                                                                                                                                        <  4:550661/274‑1 (MQ=255)

                                                                     aaTACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCCGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGGTACCAACAAGGTTTCCAGATATCGCAACCTtc                                                                                                                                                                                   >  2:102760/1‑274 (MQ=255)

                                                                            aTGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCCTCCTCCcg                                                                                                                                                                             >  2:550663/1‑273 (MQ=255)

                                                                                   gtctgtggcgATGCCCACTTTACCAAAAAGCAAGGATGGAACGTCCTTACGCGGGGTTTTTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGcc                                                                                                                                                                    <  4:140007/268‑1 (MQ=255)

                                                                                         cgcgATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCAACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCtt                                                                                                                                                                        >  2:140008/1‑265 (MQ=255)

                                                                                                  cACTTTACCCCAACGAAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCCAGCAACTCGGCACCATTCAGACGGAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTgag                                                                                                                                                     <  4:568875/275‑1 (MQ=255)

                                                                                                                  aaGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGt                                                                                                                                                                                                                                                                   >  7:181319/1‑149 (MQ=255)

                                                                                                                    ggATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCATAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTAt                                                                                                                                                                                                                                                                 <  7:628471/149‑1 (MQ=255)

                                                                                                                          aaCGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCa                                                                                                                                                                                                                                                           >  8:330654/1‑149 (MQ=255)

                                                                                                                           aCGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCa                                                                                                                                                                                                                                                           >  6:750585/1‑148 (MQ=255)

                                                                                                                           aCGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGATACCACGCa                                                                                                                                                                                                                                                           <  5:360308/148‑1 (MQ=255)

                                                                                                                             gTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGa                                                                                                                                                                                                                                                         <  6:6134/148‑1 (MQ=255)

                                                                                                                              tCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCa                                                                                                                                                                                                                       <  2:591674/181‑1 (MQ=255)

                                                                                                                              tCCTTACGCGTGGTTCCTTTATCACGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCCCCCGAGACAACAACAAAGTTCCCAAGTATCGTAAGCATCCTCCCGCCCTTAGCCAACGACCAGCTTAAGGAGTTTAGCTGGTCCATAACTCaactc                                                                                                                          >  4:110789/1‑270 (MQ=255)

                                                                                                                                cTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACg                                                                                                                       <  2:604803/275‑1 (MQ=255)

                                                                                                                                      cgTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATgccagcca                                                                                                                                                                                                                                                >  6:507589/1‑148 (MQ=255)

                                                                                                                                        tGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCcaca                                                                                                                                                                                                                                              <  5:452433/148‑1 (MQ=255)

                                                                                                                                            ttCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCcgg                                                                                                                                                                                                                                         <  7:36479/149‑1 (MQ=255)

                                                                                                                                            ttCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCcgg                                                                                                                                                                                                                                         >  8:19298/1‑149 (MQ=255)

                                                                                                                                            ttCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCcg                                                                                                                                                                                                                                          >  6:237402/1‑148 (MQ=255)

                                                                                                                                                  aTCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTg                                                                                                                                                                                                                                    <  5:237402/148‑1 (MQ=255)

                                                                                                                                                   tCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGa                                                                                                                                                                                                                                   >  5:233110/1‑148 (MQ=255)

                                                                                                                                                        cAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTgttaatca                                                                                                                                                                                                                             <  8:275385/149‑8 (MQ=255)

                                                                                                                                                        cAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAac                                                                                                                                                                                                                              >  6:322952/1‑148 (MQ=255)

                                                                                                                                                         aaCTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAaca                                                                                                                                                                                                                             <  6:742238/148‑1 (MQ=255)

                                                                                                                                                            tcaacacCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTACGTGGTATCGCGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGc                                                                                                                                                                                                                         >  8:69827/4‑149 (MQ=255)

                                                                                                                                                              gACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGATACCACGCAggt                                                                                                                                                                                                                                                        >  8:395735/1‑114 (MQ=255)

                                                                                                                                                               acacCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCa                                                                                                                                                                                                                       >  6:719651/1‑148 (MQ=255)

                                                                                                                                                                cacCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCaga                                                                                                                                                                                                                     <  7:330654/149‑1 (MQ=255)

                                                                                                                                                                  ccATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCagag                                                                                                                                                                                                                    >  6:782123/1‑148 (MQ=255)

                                                                                                                                                                  ccATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAgaga                                                                                                                                                                                                                   <  8:496294/149‑1 (MQ=255)

                                                                                                                                                                     ttCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATa                                                                                                                                                                                                                 <  5:719651/148‑1 (MQ=255)

                                                                                                                                                                     ttCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTccc                                                                                                                                                                              >  2:264022/1‑183 (MQ=255)

                                                                                                                                                                        aGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATaaca                                                                                                                                                                                                              >  5:790310/1‑148 (MQ=255)

                                                                                                                                                                          aCGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGa                                                                                                                                                                                                                                                         >  6:806306/1‑103 (MQ=255)

                                                                                                                                                                          aCGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGa                                                                                                                                                                                                                                                         <  5:806306/103‑1 (MQ=255)

                                                                                                                                                                          aCGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAacaaca                                                                                                                                                                                                           >  7:108438/1‑149 (MQ=255)

                                                                                                                                                                              aaCTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCTCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGc                                                                           <  2:90919/273‑1 (MQ=255)

                                                                                                                                                                               aCTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGtt                                                                                                                                                                                                      <  7:859114/149‑1 (MQ=255)

                                                                                                                                                                               aCTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGt                                                                                                                                                                                                       <  6:413157/148‑1 (MQ=255)

                                                                                                                                                                               aCTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGt                                                                                                                                                                                                       >  6:187396/1‑148 (MQ=255)

                                                                                                                                                                                 tGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTg                                                                                                                                                                                                     <  5:802273/148‑1 (MQ=255)

                                                                                                                                                                                 tGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGc                                                                                                                                                                                                    >  7:508887/1‑149 (MQ=255)

                                                                                                                                                                                 tGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGc                                                                                                                                                                                                    >  7:678871/1‑149 (MQ=255)

                                                                                                                                                                                   gTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGcc                                                                                                                                                                                                   >  6:284127/1‑148 (MQ=255)

                                                                                                                                                                                      aCGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGat                                                                                                                                                                                               >  8:829395/1‑149 (MQ=255)

                                                                                                                                                                                              cacGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTa                                                                                                                                       >  2:536437/1‑197 (MQ=255)

                                                                                                                                                                                                 ggCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCtt                                                                                                                                                                                    >  7:846745/1‑149 (MQ=255)

                                                                                                                                                                                                             cGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCt                                                                                                                                                                         >  5:676061/1‑148 (MQ=255)

                                                                                                                                                                                                             cGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTa                                                                                                                                              >  2:724456/1‑175 (MQ=255)

                                                                                                                                                                                                               ttCGGATCGCCTGAGTCCAGGCTCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACAcc                                                                                                                                                                                                                           >  2:1245/1‑96 (MQ=255)

                                                                                                                                                                                                               ttCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATCttt                                                                                               >  1:161128/1‑220 (MQ=255)

                                                                                                                                                                                                                tCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACg                                                                                                                       <  4:601247/195‑1 (MQ=255)

                                                                                                                                                                                                                 cGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCcggcg                                                                                                                                                                                                                                       <  3:194488/82‑1 (MQ=255)

                                                                                                                                                                                                                  ggATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGATACCACGCAg                                                                                                                                                                                                                                                          <  7:672413/62‑1 (MQ=255)

                                                                                                                                                                                                                  ggATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGATACCACGCAg                                                                                                                                                                                                                                                          >  8:672413/1‑62 (MQ=255)

                                                                                                                                                                                                                    aTCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCcg                                                                                                                                                                             >  1:92955/1‑137 (MQ=255)

                                                                                                                                                                                                                    aTCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCCATTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTACGTTGCGTCCACCg                                    >  2:315574/1‑274 (MQ=255)

                                                                                                                                                                                                                        ccTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGAcc                                                                                                                                                             <  7:417087/149‑1 (MQ=255)

                                                                                                                                                                                                                         cTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGtt                                                                                                                                                                                                      >  7:727005/1‑107 (MQ=255)

                                                                                                                                                                                                                         cTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTtc                                                                                                                                                                                   <  5:425728/126‑1 (MQ=255)

                                                                                                                                                                                                                         cTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTtc                                                                                                                                                                                   >  6:425728/1‑126 (MQ=255)

                                                                                                                                                                                                                         cTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTAACAGATGTTCCTGGCCAGCACGTTCGCGAACCTGATCTTCCGTTGCGTCCACCGGAACgg                              >  1:8677/1‑275 (MQ=255)

                                                                                                                                                                                                                             gTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGAtt                                                                                                                                                        <  8:181319/149‑1 (MQ=255)

                                                                                                                                                                                                                                cAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTgag                                                                                                                                                     <  8:508887/149‑1 (MQ=255)

                                                                                                                                                                                                                                cAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTgag                                                                                                                                                     <  7:40473/149‑1 (MQ=255)

                                                                                                                                                                                                                                    aTCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTgaggag                                                                                                                                                  >  5:306049/1‑148 (MQ=255)

                                                                                                                                                                                                                                       aTGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCa                                                                                                                                                                   >  4:728609/1‑128 (MQ=255)

                                                                                                                                                                                                                                        tGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGAt                                                                                                                                                                                                                  >  2:786310/1‑80 (MQ=255)

                                                                                                                                                                                                                                        tGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCa                                                                                                                                                                   >  1:204377/1‑127 (MQ=255)

                                                                                                                                                                                                                                        tGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACgg                >  2:62124/1‑274 (MQ=255)

                                                                                                                                                                                                                                         gACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTAc                                                                                                                                             >  5:486721/1‑148 (MQ=255)

                                                                                                                                                                                                                                          aCCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTAcc                                                                                                                                            <  6:116252/148‑1 (MQ=255)

                                                                                                                                                                                                                                           ccTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGa                                                                                                                                                               <  2:728611/128‑1 (MQ=255)

                                                                                                                                                                                                                                            cTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATaaca                                                                                                                                                                                                              <  4:786308/80‑1 (MQ=255)

                                                                                                                                                                                                                                            acgcacagtaggaggaaCCCGTGGGGTATCACGCAGCTGTCAGCCACACCCGGAGGTGAGTAACAACGCCAGAGATAACATCAATGTTGCCAGATTTCGCACGTGTCAGCCCGGGGTTAGGCAACGACCAGATTGAGGAGTGTACCTGGTACGTAAAACACTTTACGTACAGTATCGCCATCAGGATATTGTGCTACTAGATGTTCCTGGCCAGAACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATGTTGGCACGGACtt            <  4:102759/262‑1 (MQ=255)

                                                                                                                                                                                                                                             ttCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGt                                                                                                                                        >  8:657426/1‑149 (MQ=255)

                                                                                                                                                                                                                                              tCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTa                                                                                                                                       <  7:218742/149‑1 (MQ=255)

                                                                                                                                                                                                                                               cATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTAc                                                                                         >  4:36055/1‑194 (MQ=255)

                                                                                                                                                                                                                                               cATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTAc         >  2:289879/1‑274 (MQ=255)

                                                                                                                                                                                                                                                  aGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATGAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCg       <  1:181359/273‑1 (MQ=255)

                                                                                                                                                                                                                                                   gTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGa                                                                                     <  2:36056/194‑1 (MQ=255)

                                                                                                                                                                                                                                                    tGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTaaa                                                                                                                                 <  8:108438/149‑1 (MQ=255)

                                                                                                                                                                                                                                                     ggaaggaaCCCGCGTGGTATCACGCAGATGCCCGCCACCCCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGGTATCGCACGCTTCCGCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTGTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCGGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTGGGCACGGACTTTACCGTTAAc  <  4:533641/275‑1 (MQ=255)

                                                                                                                                                                                                                                                        aggaaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCAc                                                                                                                             <  8:846745/149‑1 (MQ=255)

                                                                                                                                                                                                                                                         ggaaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTAc                                                                                                                                             <  5:140662/132‑1 (MQ=255)

                                                                                                                                                                                                                                                          gaaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACt                                                                                                                            <  5:763119/148‑1 (MQ=255)

                                                                                                                                                                                                                                                             tcTGCGTGATACCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGaaaa                                                                                                                                 <  7:131997/139‑1 (MQ=255)

                                                                                                                                                                                                                                                             ccTGCGTAGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTATCACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTAc                                                                                                                        <  7:778984/149‑1 (MQ=255)

                                                                                                                                                                                                                                                              cTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACg                                                                                                                       >  7:554338/1‑149 (MQ=255)

                                                                                                                                                                                                                                                               tGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTAc                                                                                                                                             <  6:110626/126‑1 (MQ=255)

                                                                                                                                                                                                                                                               tGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTAc                                                                                                                                             >  5:110626/1‑126 (MQ=255)

                                                                                                                                                                                                                                                                gCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGt                                                                                                                      >  6:214374/1‑148 (MQ=255)

                                                                                                                                                                                                                                                                gCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTa                                                                                                                     >  7:5702/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                     gTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCcgc                                                                                                                                                                            >  2:380907/1‑89 (MQ=255)

CGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGCCAGCACGTTCGCGAACCTGTTCTTCCGTTGCGTCCACCGGAACGGTGATTTTGGCACGGACTTTACCGTTAAC  >  NC_000913/671849‑672368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`