Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,064,161 G→A 5.4% L42L (CTG→CTA) ‡ yccE → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,064,1610GA 5.4% 604.9 / 16.9 185L42L (CTG→CTA) ‡yccEuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (93/82);  new base A (5/5);  total (98/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-04

Only 100 of 185 total aligned reads displayed.

GTGAGCGATTTTTGATAGTTGAACCAGGCACTTTAAGTTTAACTAGGGCGTCATTATTTATTAAATTTTATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGGGGTTTCAGGAGGTATTACATATACTATACAATATGTTCGAGATATTGATATTGTTAGAGTGTCCTTGCCGGGCAGAGCTTCA  >  NC_000913/1063954‑1064413
                                                                                                                                                                                                               |                                                                                                                                                                                                                                                            
gTGAGCGATTTTTGATAGTTGAACCAGGCACTTTAAGTTTAACTAGGGCGTCATTATTTATTAAATTTTATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTGTTAt                                                                                                                                                                                                                                                   >  4:169137/1‑219 (MQ=255)
    gCGATTTTTGATAGTTGAACCAGGCACTTTAAGTTTAACTAGGGCGTCATTATTTATTAAATTTTATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCgg                                                                                                                                                                                                                                               <  2:169138/219‑1 (MQ=255)
                                                                    tataGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACCAGTGTGttt                                                                                                                                                                                                                                                      <  5:410690/148‑1 (MQ=255)
                                                                                atatGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATAc                                                                                                                                                                                                                                         <  7:220517/149‑1 (MQ=255)
                                                                                     ggTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGgtgtgt                                                                                                                                                                                                                                                        >  3:198576/1‑129 (MQ=255)
                                                                                         gtaATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTa                                                                                                                                                                                                                                                    <  1:198577/129‑1 (MQ=255)
                                                                                         gtaATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGAc                                                                                                                                                                                                                            >  3:94809/1‑153 (MQ=255)
                                                                                         gtaATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgt                                                                                                                                                                                                                          >  3:117309/1‑155 (MQ=255)
                                                                                         gtaATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAAtgtg                                                                                                                                                                                     >  3:38547/1‑192 (MQ=255)
                                                                                         gtaATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGaaa                                                                                                                                                          >  2:128372/1‑219 (MQ=255)
                                                                                             tataCATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgtaa                                                                                                                                                                                                                        <  1:94810/153‑1 (MQ=255)
                                                                                             tataCATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATc                                                                                                                                                                                 <  1:38548/192‑1 (MQ=255)
                                                                                             tataCATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAg                                                                                                                                                                      <  2:345063/203‑1 (MQ=255)
                                                                                             tataCATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCa                                                                                                                                                      <  4:128371/219‑1 (MQ=255)
                                                                                               taCATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATCAAATGAACTACGACGTAAAGTAAATGAAGAGGATACTCCATGTATGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAAGGATAAGGATCTATTATTATTTATGAGAATATTGCGTACTAATAATACACAAAtt                                                                                            >  4:209506/1‑275 (MQ=255)
                                                                                               taCATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAAANNNTAGa                                                                                                                                                                           >  4:788726/1‑196 (MQ=255)
                                                                                               taCATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGAAGTAAAGTAAATGAag                                                                                                                                                                                                             >  4:712656/1‑162 (MQ=255)
                                                                                               taCATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACAAACTGGAGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAg                                                                                                                                                                            >  1:101185/1‑195 (MQ=255)
                                                                                                   tGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgtaaag                                                                                                                                                                                                                      >  8:380264/1‑149 (MQ=255)
                                                                                                   tGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGa                                                                                                                                                                                                         <  2:712658/162‑1 (MQ=255)
                                                                                                   tGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGaaaaa                                                                                                                                                                       <  2:788728/196‑1 (MQ=255)
                                                                                                    ggAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGAc                                                                                                                                                                                                                            >  2:279776/1‑142 (MQ=255)
                                                                                                    ggAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGAc                                                                                                                                                                                                                            >  2:760842/1‑142 (MQ=255)
                                                                                                    ggAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGa                                                                                                                                                            >  4:177294/1‑206 (MQ=255)
                                                                                                    ggAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAAt                                                                                                                                                         >  2:333137/1‑209 (MQ=255)
                                                                                                    ggAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatg                                                                                                                                                 >  4:457183/1‑217 (MQ=255)
                                                                                                    ggAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaat                                                                                                                                                  >  4:481954/1‑216 (MQ=255)
                                                                                                    ggAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATgag                                                                                                                        >  4:432043/1‑242 (MQ=255)
                                                                                                    ggAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTAAAGCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACATTGAACTCAGAc                                                                                                                                                                                                                            >  4:278222/1‑142 (MQ=255)
                                                                                                        ttAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgtaa                                                                                                                                                                                                                        <  4:760840/142‑1 (MQ=255)
                                                                                                        ttAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAAt                                                                                                                                                                                                                 >  8:721779/1‑149 (MQ=255)
                                                                                                        ttAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGaaa                                                                                                                                                                         <  1:102326/189‑1 (MQ=255)
                                                                                                        ttAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAg                                                                                                                                                     <  4:333135/209‑1 (MQ=255)
                                                                                                        ttAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatgata                                                                                                                                              <  2:481956/216‑1 (MQ=255)
                                                                                                        ttAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaa                                                                                                                                                   <  1:160937/211‑1 (MQ=255)
                                                                                                         tAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACaa                                                                                                                                                                                                                                       >  5:305569/1‑126 (MQ=255)
                                                                                                         tAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACaa                                                                                                                                                                                                                                       <  6:305569/126‑1 (MQ=255)
                                                                                                              gCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatgata                                                                                                                                              <  4:281118/210‑1 (MQ=255)
                                                                                                              gCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatg                                                                                                                                                 >  4:552266/1‑207 (MQ=255)
                                                                                                               cACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGaa                                                                                                                                                                                                              >  3:206670/1‑145 (MQ=255)
                                                                                                                aCTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGgtgtgt                                                                                                                                                                                                                                                        >  2:258468/1‑102 (MQ=255)
                                                                                                                   gCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAgg                                                                                                                                                                                                          <  1:206671/145‑1 (MQ=255)
                                                                                                                    cAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTa                                                                                                                                                                                                                                                    <  4:258467/102‑1 (MQ=255)
                                                                                                                    cAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgtaaa                                                                                                                                                                                                                       >  2:527998/1‑131 (MQ=255)
                                                                                                                        taatTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgtaaagtaa                                                                                                                                                                                                                   <  4:527996/131‑1 (MQ=255)
                                                                                                                              tttGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGgtgtgt                                                                                                                                                                                                                                                        >  2:49269/1‑88 (MQ=255)
                                                                                                                               ttGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTgg                                                                                                                                                                                           >  5:772733/1‑148 (MQ=255)
                                                                                                                               ttGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACCAGTGTGtttt                                                                                                                                                                                                                                                     >  5:178411/1‑90 (MQ=255)
                                                                                                                               ttGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACCAGTGTGtttt                                                                                                                                                                                                                                                     <  6:178411/90‑1 (MQ=255)
                                                                                                                                tGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgtaaa                                                                                                                                                                                                                       >  1:143159/1‑119 (MQ=255)
                                                                                                                                  aaaCAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTa                                                                                                                                                                                                                                                    <  4:49268/88‑1 (MQ=255)
                                                                                                                                  aaaCAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTa                                                                                                                                                                                                                                                    <  4:796370/88‑1 (MQ=255)
                                                                                                                                   aaCAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAAtgt                                                                                                                                                                                      >  7:82622/1‑149 (MQ=255)
                                                                                                                                   aaCAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAAtg                                                                                                                                                                                       <  6:622734/148‑1 (MQ=255)
                                                                                                                                    aCAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgtaaagtaa                                                                                                                                                                                                                   <  3:143158/119‑1 (MQ=255)
                                                                                                                                      aGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGa                                                                                                                                                                                    >  6:780227/1‑148 (MQ=255)
                                                                                                                                       ggCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAg                                                                                                                                                              <  4:197202/169‑1 (MQ=255)
                                                                                                                                             gAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGttt                                                                                                                                                                                                                                                      >  1:57672/1‑75 (MQ=255)
                                                                                                                                             gAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAg                                                                                                                                                                            <  7:870761/149‑1 (MQ=255)
                                                                                                                                             gAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatgat                                                                                                                                               >  2:406215/1‑178 (MQ=255)
                                                                                                                                               aCGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGaa                                                                                                                                                                                                                                   >  8:138345/1‑92 (MQ=255)
                                                                                                                                               aCGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGaa                                                                                                                                                                                                                                   <  7:138345/92‑1 (MQ=255)
                                                                                                                                                cGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGaa                                                                                                                                                                          >  6:148506/1‑148 (MQ=255)
                                                                                                                                                 gATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGgt                                                                                                                                                                                                                                                            <  2:746268/65‑1 (MQ=255)
                                                                                                                                                  atatAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGaaaa                                                                                                                                                                        <  6:322489/148‑1 (MQ=255)
                                                                                                                                                  atatAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGaaaa                                                                                                                                                                        <  5:389793/148‑1 (MQ=255)
                                                                                                                                                  atatAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTACTGATACCCAAATTTATTAAGGGGTTTTCGGAGGTATTACATATACTATCCAATCTGTTAGAgat                                         >  2:591540/1‑275 (MQ=255)
                                                                                                                                                     taAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGaa                                                                                                                                                                                   <  7:94137/134‑1 (MQ=255)
                                                                                                                                                      aaaaaaTGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgtaaa                                                                                                                                                                                                                       <  7:237161/97‑1 (MQ=255)
                                                                                                                                                      aaaaaaTGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgtaaa                                                                                                                                                                                                                       >  8:237161/1‑97 (MQ=255)
                                                                                                                                                      aaaaaaTGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGa                                                                                                                                                                                                         <  2:803124/111‑1 (MQ=255)
                                                                                                                                                          aaTGAGTAAGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGa                                                                                                                                                               <  8:92524/149‑1 (MQ=255)
                                                                                                                                                           aTGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAg                                                                                                                                                              >  7:340337/1‑149 (MQ=255)
                                                                                                                                                               gTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATg                                                                                                                                                                                                                                     >  4:817663/1‑74 (MQ=255)
                                                                                                                                                                tACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAAt                                                                                                                                                         >  8:294517/1‑149 (MQ=255)
                                                                                                                                                                tACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAAt                                                                                                                                                         >  4:754001/1‑149 (MQ=255)
                                                                                                                                                                tACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATg                                                                                                                                                        <  4:367601/150‑1 (MQ=255)
                                                                                                                                                                tACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGGGGTTTCAGGAGGTATTACATATACTATACAATATGTTCGAGATATTGATATTGTTag                           >  2:438480/1‑275 (MQ=255)
                                                                                                                                                                 aCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTc                                                                                                                                                                                              <  2:183521/111‑1 (MQ=255)
                                                                                                                                                                    aaaaaaaTCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAAt                                                                                                                                                         >  2:98907/1‑145 (MQ=255)
                                                                                                                                                                    aaaaaaaTCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAg                                                                                                                                                     <  2:754003/149‑1 (MQ=255)
                                                                                                                                                                        aaaTCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTAATGATaca                                                                                                  <  4:593379/196‑1 (MQ=255)
                                                                                                                                                                         aaTCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatg                                                                                                                                                 <  5:125409/148‑1 (MQ=255)
                                                                                                                                                                              aaCATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatgataatg                                                                                                                                           >  7:638649/1‑149 (MQ=255)
                                                                                                                                                                              aaCATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatgataat                                                                                                                                            >  6:584543/1‑148 (MQ=255)
                                                                                                                                                                                cATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatgataatgat                                                                                                                                         <  7:721779/149‑1 (MQ=255)
                                                                                                                                                                                    ttCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatgataatgat                                                                                                                                         <  5:597899/145‑1 (MQ=255)
                                                                                                                                                                                    ttCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTa                                                                                                                                      <  5:627072/148‑1 (MQ=255)
                                                                                                                                                                                        aTCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCatt                                                                                                                                 >  7:175402/1‑149 (MQ=255)
                                                                                                                                                                                         tCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGGGGTTTCAGGAGGTATTACATATACTATACAATATGTTCGAGATATTGATATTGTTAGAGTGTCCTTGCCGGGCAGAGCTTCa  <  2:27252/275‑1 (MQ=255)
                                                                                                                                                                                             gCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGGGGTTTCAGGAGGTATTACATATACTATACAATATGTTCgag                                           >  4:74690/1‑230 (MQ=255)
                                                                                                                                                                                              cTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCAttatttat                                                                                                                           <  8:149682/149‑1 (MQ=255)
                                                                                                                                                                                              cTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGGGGttt                                                                               >  2:476610/1‑193 (MQ=255)
                                                                                                                                                                                                ttttGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGc                                                                                                                                                       <  6:837190/119‑1 (MQ=255)
                                                                                                                                                                                                ttttGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGc                                                                                                                                                       >  5:837190/1‑119 (MQ=255)
                                                                                                                                                                                                 tttGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAaga                                                                                                                                                                                                            >  2:203616/1‑65 (MQ=255)
                                                                                                                                                                                                 tttGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGGGGTTTCAGGAGGTATTACATATACTATACAATATGTTCGAgatat                                       <  2:74691/230‑1 (MQ=255)
                                                                                                                                                                                                    taaaaCACACCAGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGaaaa                                                                                                                                                                        >  6:424507/2‑98 (MQ=255)
                                                                                                                                                                                                       aaCACACCAGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAatat                                                                                                                   >  5:513651/1‑148 (MQ=255)
                                                                                                                                                                                                            acTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTa                                                                                                             >  7:387118/1‑149 (MQ=255)
                                                                                                                                                                                                               |                                                                                                                                                                                                                                                            
GTGAGCGATTTTTGATAGTTGAACCAGGCACTTTAAGTTTAACTAGGGCGTCATTATTTATTAAATTTTATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGGGGTTTCAGGAGGTATTACATATACTATACAATATGTTCGAGATATTGATATTGTTAGAGTGTCCTTGCCGGGCAGAGCTTCA  >  NC_000913/1063954‑1064413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: