Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,224,239 A→T 5.3% intergenic (+332/+40) ycgI → / ← minE pseudogene/cell division topological specificity factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,224,2390AT 5.3% 606.2 / 17.7 169intergenic (+332/+40)ycgI/minEpseudogene/cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base A (80/80);  new base T (6/3);  total (86/83)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-02

Only 100 of 170 total aligned reads displayed.

AACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTT  >  NC_000913/1224005‑1224487
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                        
aaccccaatcTATGGGGGAACAATTATTAATGAAATTGCGGGGGTATCATCTCCCTGTATTTCCATTCTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGGTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCATGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCt                                                                                                                                                                                                                  <  4:78625/266‑1 (MQ=255)
aaCCTCATCCCATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATAGCCATACTAACAATAAGGTTATTTACTCAAACAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCt                                                                                                                                                                                                                  <  2:631458/275‑1 (MQ=255)
                         aaaaatGAAATTGCGGGGTTTTCACCTACCATTATATCCATACTAACAATAAGTTTATTTACTAAACCAGGCATAAACATTTTGTTTTGTGCGGGGGAACAGCCTTAAGGGGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTa                                                                                                                                                                                         <  4:41468/272‑1 (MQ=255)
                              tGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTcc                                                                                                                                                                                             >  2:41469/1‑266 (MQ=255)
                                                   tcCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCGGCTTTACCAAAACGTGGATGAGAGGGATGAAAAACAAAAGGCAGAGAGAACGCTGCCTTGAAGGTAAATGCGGTTTTAAAGCGGGCTTATTTCAGCACTTCTGCTTCCNGTAAGNNTAAGTTTAGGTCAAAAATATaa                                                                                                                                                               >  3:13734/1‑275 (MQ=255)
                                                     ccAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtcttc                                                                                                                                                                                                    >  2:430805/1‑236 (MQ=255)
                                                         taaatCCAAACTAACAATAAGGTGATTTACTCAACCAGGCATAAACAGTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCt                                                                                                                                                                                                <  4:430803/233‑1 (MQ=255)
                                                             taagggacagcAATAAGGTTTTTTAACCAACCAGGACTAAAACTTTTTTTTTTTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGCGACTCAGCAAGAATACTCTCCGCTTTACGACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCa                                                                                                                                                      <  4:544872/264‑1 (MQ=255)
                                                                                        cAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGAt                                                                                                                                                                                                                                                         >  6:266859/1‑148 (MQ=255)
                                                                                         aaCCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATaa                                                                                                                                                                                                                                                       >  7:844118/1‑149 (MQ=255)
                                                                                         aaCCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATaa                                                                                                                                                                                                                                                       >  7:395477/1‑149 (MQ=255)
                                                                                            cAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTAtctc                                                                                                                                                                                                                                                    <  8:448766/149‑1 (MQ=255)
                                                                                              ggCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATa                                                                                                                                                                                                                                       >  4:342106/1‑160 (MQ=255)
                                                                                               gCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGc                                                                                                                                                                                                                                                 >  7:238728/1‑149 (MQ=255)
                                                                                               gCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGc                                                                                                                                                                                                                                                 <  7:434153/149‑1 (MQ=255)
                                                                                                  tAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCt                                                                                                                                                                                                                                               <  5:349487/148‑1 (MQ=255)
                                                                                                  tAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATg                                                                                                                                                                                                                                   <  2:342108/160‑1 (MQ=255)
                                                                                                   aaaCATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTg                                                                                                                                                                                                                                             >  8:521185/1‑149 (MQ=255)
                                                                                                       aTTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGa                                                                                                                                                                                                                                         <  7:313681/149‑1 (MQ=255)
                                                                                                         tttgttttgtGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGAt                                                                                                                                                                                                                                        >  6:19954/1‑148 (MQ=255)
                                                                                                            gttttgtGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAAt                                                                                                                                                                                                                                    <  7:94405/149‑1 (MQ=255)
                                                                                                            gttttgtGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAAt                                                                                                                                                                                                                                    <  8:844118/149‑1 (MQ=255)
                                                                                                              ttggggCGTGGGAAAAGCCTTAAGGTGTAAAGGGGGAGGGTGAAATAGCAATGAGTAGTATCAGAAATAATACTCGGCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGt                                                                                                     <  4:644633/269‑1 (MQ=255)
                                                                                                                tgtgCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATgcgc                                                                                                                                                                                                                                <  8:633079/149‑1 (MQ=255)
                                                                                                                tgtgCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATgcg                                                                                                                                                                                                                                 <  6:451930/148‑1 (MQ=255)
                                                                                                                 gtgCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTGCAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTAttt                                                                                                 >  2:311960/1‑275 (MQ=255)
                                                                                                                  taggTGGGAAAAGCCCTAAGGTGTAGAAGAGGAAGTTGAAATAGCAATGACGAGTATCAGCAAGGATACTGGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGTCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGTTAAGGTCACGTTCAGCTCAAGAATAGCAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGt                                                                                                     <  4:407407/267‑1 (MQ=255)
                                                                                                                   gCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCtt                                                                                                                                                                                                                              <  5:59570/148‑1 (MQ=255)
                                                                                                                     gTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAGACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTa                                                                                                                                                                                                                           <  8:395477/149‑1 (MQ=255)
                                                                                                                           aCAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCg                                                                                                                                                                                                                      <  6:344766/148‑1 (MQ=255)
                                                                                                                              gCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGGATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATTAAAAACTCAACGCAGAGATAACTCTGCCTTGACGATAAATGCGCTTTTACAGCgagt                                                                                                                                                                                                                   >  6:572851/1‑145 (MQ=255)
                                                                                                                               ccTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAATAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAACCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCt                                                                                                                                                                                                                  <  5:468280/148‑1 (MQ=255)
                                                                                                                                cTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCt                                                                                                                                                                                                                                               <  6:319376/118‑1 (MQ=255)
                                                                                                                                cTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCt                                                                                                                                                                                                                                               >  5:319376/1‑118 (MQ=255)
                                                                                                                                cTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCtt                                                                                                                                                                                                                 >  5:435784/1‑148 (MQ=255)
                                                                                                                                 ctAAGGGGGAAAGGGGGAGGGGGAAATAAGAATGAGGAGTATCAGCAAGAATACTCGGCGCTTTATCAAAACGTTGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTGCAGCGTGCTTATTTCAGCTCTTCTGCTTCCGGTAATGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTGTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAg                                                                                  <  4:311959/273‑1 (MQ=255)
                                                                                                                                         tAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTAtt                                                                                                                                                                                                              >  2:118653/1‑142 (MQ=255)
                                                                                                                                          aaaGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGcc                                                                                                                                                                                                                                                <  5:4087/107‑1 (MQ=255)
                                                                                                                                              ggggAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCa                                                                    >  1:216957/1‑275 (MQ=255)
                                                                                                                                               gggAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTg                                                                                                                                                                                                  >  5:846548/1‑148 (MQ=255)
                                                                                                                                                 gAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATa                                                                                                                                                                                                                                       >  1:117727/1‑109 (MQ=255)
                                                                                                                                                 gAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGt                                                                                                                                                                                >  2:103159/1‑164 (MQ=255)
                                                                                                                                                   ggTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTg                                                                                                                                                                                                  <  5:832214/144‑1 (MQ=255)
                                                                                                                                                     tGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATg                                                                                                                                                                                                                                   <  3:117726/109‑1 (MQ=255)
                                                                                                                                                     tGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCg                                                                                                                                                                                            >  6:842253/1‑148 (MQ=255)
                                                                                                                                                     tGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAg                                                                                                                                                                            <  4:103158/164‑1 (MQ=255)
                                                                                                                                                     tGGAAATAGCAATGAGGAGTAGCAGCATGAATACTCGACGCTTTACCACAACGTGGATGAGAGTGATGAAAAACTCAAGGCAGAGATAACTCTGGCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAAGTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCg                                                              <  1:218187/274‑1 (MQ=255)
                                                                                                                                                       gAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCAGGCTTATTTCAGCTCTTCTGCTTCCGGTa                                                                                                                                                                                         <  7:538950/149‑1 (MQ=255)
                                                                                                                                                        aaaTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtcttc                                                                                                                                                                                                    >  5:14046/1‑137 (MQ=255)
                                                                                                                                                        aaaTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTaa                                                                                                                                                                                        <  7:806906/149‑1 (MQ=255)
                                                                                                                                                         aaTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGa                                                         <  2:373377/275‑1 (MQ=255)
                                                                                                                                                          aTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAg                                                                                                                                                                                       <  5:784129/148‑1 (MQ=255)
                                                                                                                                                              cAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTTTTCTGCTTTCGGTACGGTCACGTTCAGCTCAAGTATAGAAATATCGCCCTCTTTTTGCTTAAGCTGTTCGGTTACCATCTCAGGCTCAAt                                                                                                             >  2:625899/1‑218 (MQ=255)
                                                                                                                                                                 tGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGtt                                                                                                                                                                               >  8:275146/1‑149 (MQ=255)
                                                                                                                                                                       gTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTc                                                                                                                                                                         <  8:75168/149‑1 (MQ=255)
                                                                                                                                                                       gTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTc                                                                                                                                                                         >  7:782380/1‑149 (MQ=255)
                                                                                                                                                                        tATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCg                                                                                                                                                                                            <  7:271872/129‑1 (MQ=255)
                                                                                                                                                                         aataataaGAATACTCGCCTCTTGTACAANNNTTGGATGAGAGGGATTAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCAt                                         <  3:216956/269‑1 (MQ=255)
                                                                                                                                                                          tCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtctt                                                                                                                                                                                                     <  1:115094/118‑1 (MQ=255)
                                                                                                                                                                                  aaTACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGc                                                                                                                                       >  2:374032/1‑172 (MQ=255)
                                                                                                                                                                                    tACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGa                                                                                                                                                                     >  5:610008/1‑140 (MQ=255)
                                                                                                                                                                                      cTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTa                                                                                                                                   <  4:374030/172‑1 (MQ=255)
                                                                                                                                                                                        cgccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGt                                                                                                                                                                                >  6:231286/1‑125 (MQ=255)
                                                                                                                                                                                        cgccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGt                                                                                                                                                                                <  5:231286/125‑1 (MQ=255)
                                                                                                                                                                                         gccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCa                                                                                                                                                                                   <  6:376936/121‑1 (MQ=255)
                                                                                                                                                                                         gccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCa                                                                                                                                                                                   >  5:376936/1‑121 (MQ=255)
                                                                                                                                                                                         gccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGcc                                                                                                                                                       >  7:573088/1‑149 (MQ=255)
                                                                                                                                                                                            gcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTa                                                                                                                                                                                         >  4:7825/1‑112 (MQ=255)
                                                                                                                                                                                             cTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAg                       <  1:156685/273‑1 (MQ=255)
                                                                                                                                                                                             atttGCCAAAACGGGGAAGAGGGGGTGGAAAAATTAAAGGCAGGGATAACTCTGCCTTGGAGATAAATGCGCTTGTAAAGTGGGTTAATTTCATCTTTTTTGTTTCCGGTAAGGTAACGTTCAGCTCAAGAATAGAAATATCGACATCTTTTTGCTCAAGCTGTACAGTTACCATCGCAGGAAGAATTTGTACGTATGTAAAAAGAACCTCAAGAATATCTTTACGCAACTGCTGAACATAATGCGGTTCTGCATCGCCGAGACGGCGTTCAg                       <  4:166099/272‑1 (MQ=255)
                                                                                                                                                                                                tACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGt                                                                                                                                                                                     <  2:7825/112‑1 (MQ=255)
                                                                                                                                                                                                 aCCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCttttt                                                                                                                                               <  8:238728/149‑1 (MQ=255)
                                                                                                                                                                                                   cacaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGc                                                                                                                                             >  8:816133/1‑149 (MQ=255)
                                                                                                                                                                                                    acaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCtttt                                                                                                                                                >  8:269509/1‑145 (MQ=255)
                                                                                                                                                                                                     caACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGt                                                                                                                                                                                     >  6:671373/1‑107 (MQ=255)
                                                                                                                                                                                                     caACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGt                                                                                                                                                                                     <  5:671373/107‑1 (MQ=255)
                                                                                                                                                                                                          tGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCttttt                                                                                                                                               >  2:113575/1‑140 (MQ=255)
                                                                                                                                                                                                            gATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCttttt                                                                                                                                               >  2:771139/1‑138 (MQ=255)
                                                                                                                                                                                                            gATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGAACAAATAATTTGCAGc      >  4:353568/1‑275 (MQ=255)
                                                                                                                                                                                                              tGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTc                                                                                                                                           <  4:113574/140‑1 (MQ=255)
                                                                                                                                                                                                              tGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTa                                                                                                                                   <  5:266859/148‑1 (MQ=255)
                                                                                                                                                                                                                agagGGATGAAAAACTCAAGGCAGGGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGtt  <  2:394131/275‑1 (MQ=255)
                                                                                                                                                                                                                agagGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTc                                                                                                                                           <  4:771137/138‑1 (MQ=255)
                                                                                                                                                                                                                agagGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACg                                                                                                                                 <  6:743666/148‑1 (MQ=255)
                                                                                                                                                                                                                 gagGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATGGCCATCTTTTTGCTCAAGCTGTACGGt                                                                                                                               <  7:420374/149‑1 (MQ=255)
                                                                                                                                                                                                                  agGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAAt                                                                                                                                                                   >  5:425822/1‑112 (MQ=255)
                                                                                                                                                                                                                  agGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAAt                                                                                                                                                                   <  6:425822/112‑1 (MQ=255)
                                                                                                                                                                                                                  agGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGtt  >  2:530560/1‑273 (MQ=255)
                                                                                                                                                                                                                    ggATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAg                                                                                                                                                                                                          <  7:69846/71‑1 (MQ=255)
                                                                                                                                                                                                                     gATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATc                                                                                                                                                    <  5:720065/124‑1 (MQ=255)
                                                                                                                                                                                                                      aTGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTAcc                                                                                                                           >  6:911604/1‑148 (MQ=255)
                                                                                                                                                                                                                         aaaaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCAtct                                                                                                                       >  8:337056/1‑149 (MQ=255)
                                                                                                                                                                                                                           aaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCAtctc                                                                                                                      <  5:331366/148‑1 (MQ=255)
                                                                                                                                                                                                                               tCAAGGCAGAGTTATCTCTGCCTTGAGGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGa                                                                                                                  >  6:406200/1‑148 (MQ=255)
                                                                                                                                                                                                                                cAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCa                                                                                                                                                                        >  6:681423/1‑93 (MQ=255)
                                                                                                                                                                                                                                 aaGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGtt                                                                                                                              >  8:423418/1‑134 (MQ=255)
                                                                                                                                                                                                                                 aaGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCa                                                                                                               >  8:742177/1‑149 (MQ=255)
                                                                                                                                                                                                                                   ggCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATaaa                                                                                                              >  5:333837/1‑148 (MQ=255)
                                                                                                                                                                                                                                   ggCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCaa                                                                                                              <  5:694309/148‑1 (MQ=255)
                                                                                                                                                                                                                                    gCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAAt                                                                                                             >  5:878336/1‑148 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                        
AACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACAATAAGGTTATTTACTCAACCAGGCATAAACATTTTGTTTTGTGCGTGGGAACAGCCTTAAGGTGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCATCTTTTTGCTCAAGCTGTACGGTTACCATCTCAGGATCAATTTGTACGTATTTACAAATGACCTCAAGAATATCTTTACGCAACTGCGGCAGATAATGCGGTTCTGCATCGCTGCGACGGCGTTCAGCAACAATAATCTGCAGCCGTT  >  NC_000913/1224005‑1224487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: