Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,541,077 | G→C | 5.2% | A505G (GCC→GGC) | narZ ← | nitrate reductase 2 (NRZ), alpha subunit |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,541,077 | 0 | G | C | 5.2% | 465.1 / 13.9 | 134 | A505G (GCC→GGC) | narZ | nitrate reductase 2 (NRZ), alpha subunit |
Reads supporting (aligned to +/- strand): ref base G (66/61); new base C (4/3); total (70/64) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.23e-02 |
Only 100 of 136 total aligned reads displayed.
CCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAA > NC_000913/1540824‑1541342 | ccAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGAttt < 1:158875/275‑1 (MQ=255) acAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAATCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGAttt < 2:558237/274‑1 (MQ=255) tttttCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCt < 2:293322/235‑1 (MQ=255) gccgccACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGc > 2:232152/1‑275 (MQ=255) gccgccACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGc > 2:637288/1‑275 (MQ=255) ccgccACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCTGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGACAAATTCACGGGCGATGGTTTCAATATACTGGCGGGGCGCGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTAAGGTTTGATTTCCG‑CTTAATCTTTCGCACTGTTTT‑CATTTTCCAGCCCGCATTCCAGGc > 4:636263/1‑274 (MQ=255) ccgccACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGc < 2:153253/274‑1 (MQ=255) cacaACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCaa > 4:331461/1‑274 (MQ=255) cAGGATAGTGATTCTGCCGCAGTTGTTCATGACCAATTGAGACCAGTGGTTAACACCTGCGGCGAGGATAATCATCGAGCGCCCATGCGTTTGAGGGGGGGTATCGGCAAATTCACGAGCGATGGGTTAAATAGGCTGGCGCGGCACGCCGGTAATTTGCTAACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGacca < 4:232151/275‑1 (MQ=255) gATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTCATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAgg > 2:42529/1‑275 (MQ=255) atcatcCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTCATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTCCCAAATCATAACCGCTACCCACCGGACAGgt > 2:293612/1‑275 (MQ=255) atcatcCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGCGGCCGTAATTCGCCAGCACCAAATCATAAACGCTGACCACCGGACAGgt > 2:8959/1‑275 (MQ=255) atcCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGgtg < 2:680600/273‑1 (MQ=255) gTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTg > 8:661644/1‑149 (MQ=255) tCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTggg < 8:177074/149‑1 (MQ=255) aTGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGTGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTgggg > 5:790479/1‑148 (MQ=255) tGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGgtg < 7:862493/149‑1 (MQ=255) tGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGgt > 6:750177/1‑148 (MQ=255) tCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGCCTGGGgtga < 6:272301/148‑2 (MQ=255) aTGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACgg > 8:800744/1‑149 (MQ=255) tgtgATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGttt > 2:628415/1‑275 (MQ=255) tgATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGttt > 7:228478/1‑149 (MQ=255) aTACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTg > 6:409693/1‑148 (MQ=255) aTACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTg < 5:13701/148‑1 (MQ=255) aTACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTg < 6:289548/148‑1 (MQ=255) tACCAGCGGTTAACACCTGCGCCGAGGATAATCATCCAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGAt < 7:800744/149‑1 (MQ=255) cAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTc > 7:658627/1‑149 (MQ=255) cAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGCCTgg < 6:189379/131‑1 (MQ=255) gTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAg < 8:73383/149‑1 (MQ=255) gTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCCCCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCa > 1:100799/1‑275 (MQ=255) ttAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTAc < 5:83321/132‑1 (MQ=255) ttAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTAc > 6:83321/1‑132 (MQ=255) tAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTaa < 7:206809/149‑1 (MQ=255) ccTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCttt < 7:76574/149‑1 (MQ=255) ccTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCAc < 2:331463/275‑1 (MQ=255) cTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCttt < 6:845015/148‑1 (MQ=255) gcgcCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGgt > 3:72735/1‑119 (MQ=255) cgcCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCcgc > 4:433199/1‑171 (MQ=255) cgcCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTg > 2:36551/1‑212 (MQ=255) ccGCGGAGAATCGTCGAGCGACCTTACGTTTTATGGTGTGTAACGGGAAATGCACGGGAGATGTTTTTGATAAATGTGGGTGGCTAGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTGTGATGTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCGTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGCTTTTAACGGGCAATTGGCGCACCAGCa < 4:637286/274‑1 (MQ=255) ccGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTAAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCa < 2:18529/274‑1 (MQ=255) cGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTAc < 1:72736/119‑1 (MQ=255) gAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATc < 2:433201/171‑1 (MQ=255) aGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCAcc > 1:14893/1‑273 (MQ=255) aTAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGtttt < 6:386333/148‑1 (MQ=255) aTAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑c < 7:831081/149‑1 (MQ=255) aaTCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CAt < 7:684141/149‑1 (MQ=255) tcatcGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACACCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATc < 6:783686/148‑1 (MQ=255) atcGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCtt < 5:549044/148‑1 (MQ=255) atcGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCtt < 5:409693/148‑1 (MQ=255) cGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTcc > 5:757474/1‑148 (MQ=255) cgcCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGccc < 8:90401/149‑1 (MQ=255) ccATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCttt > 4:748115/1‑122 (MQ=255) aTGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGa < 6:431503/148‑1 (MQ=255) aTGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGAt > 7:139012/1‑149 (MQ=255) gggCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAg > 8:510044/1‑99 (MQ=255) tATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGcc < 8:228478/149‑1 (MQ=255) aTCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCa < 8:139012/149‑1 (MQ=255) ggCAAATTCACGGGCGATGGTTTCAATATACTGGTGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTa > 8:301004/1‑149 (MQ=255) ggCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGt < 5:123572/79‑1 (MQ=255) cAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATGCGCCAGTAc > 5:7250/1‑148 (MQ=255) gggCGATTGTTTCAATATACTGGCGCTGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAgggg < 1:180854/275‑1 (MQ=255) ggCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTc > 6:63615/1‑75 (MQ=255) ggCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTc < 5:63615/75‑1 (MQ=255) ggCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATaaa > 8:646429/1‑149 (MQ=255) ggCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATaaa < 8:649495/149‑1 (MQ=255) gATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGc > 7:24083/1‑149 (MQ=255) ggTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGa < 8:481445/149‑1 (MQ=255) gTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGac < 7:307782/149‑1 (MQ=255) atatACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCgg > 7:148119/1‑149 (MQ=255) tataCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCgg > 6:353426/1‑148 (MQ=255) tataCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGa > 7:143541/1‑149 (MQ=255) taCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACa < 7:431890/149‑1 (MQ=255) aCTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAAt > 4:650122/1‑111 (MQ=255) tGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGa > 4:467856/1‑96 (MQ=255) tGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAgg > 5:216893/1‑148 (MQ=255) tGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAgg > 6:55656/1‑148 (MQ=255) ggCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATaa < 6:367464/128‑1 (MQ=255) ggCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATaa > 5:367464/1‑128 (MQ=255) ggCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGc < 2:612967/274‑1 (MQ=255) gcgcGGCCCGCC‑GTAATTTGCTCCCCCCCGGCTGGGGGGTAAGGTTTTGTTTCAGCCGTAA‑CTTTCGGCATGTTTTCCATC‑TCCAGCCCGGCAATGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGtt < 8:713376/149‑1 (MQ=255) gcgcGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGc < 2:650124/111‑1 (MQ=255) cgcgGCACGCCGGTAATTTGCTCACTCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCCGAACCAAATCATAAACGCTGACCACCGGACAGGCGTTGCCATCGACTACCGTCAGGTTTTTAACGGCCGATTGGCGCCTCAGACCCGGATTGTTTATAACGCTGCGAAAAAGCGGATTTCCAATGCTGCCAAAGGCGGGGAAGGCCACGCCTGCAACAggacc > 4:372872/1‑271 (MQ=255) gcgGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGa < 2:467858/96‑1 (MQ=255) cgGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCAACAGCACCGGATTGTGTTTTAAGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCACCGCCTGCAACacactaat > 4:48071/1‑268 (MQ=255) gcagcccGGTAATTTGCTCACCCCACGCTGTGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGAACGAGGCCTTAATTCGCCAGTACCAAATCATAAGCGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCaa < 2:372874/259‑1 (MQ=255) cGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATc > 6:767201/1‑148 (MQ=255) gCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGa < 7:661644/149‑1 (MQ=255) tAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTaa < 5:487209/148‑1 (MQ=255) aTTTTCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAAGTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCa < 1:18373/274‑1 (MQ=255) tttGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCTAGGCCGTAATTCGCCAGTACCAAATCATAATCGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTc > 8:672775/1‑149 (MQ=255) gtacACCCCAGCCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAg > 5:624977/4‑148 (MQ=255) tacACCCCAGCCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGt > 8:253327/3‑149 (MQ=255) tCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGtt > 8:713551/1‑149 (MQ=255) cACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGtt > 6:806825/1‑148 (MQ=255) aCCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGtttt > 8:643805/1‑149 (MQ=255) ccccAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGttttt > 8:720911/1‑149 (MQ=255) ccccAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGtttt > 5:97983/1‑148 (MQ=255) ccAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTa > 6:10537/1‑148 (MQ=255) ggCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACg < 6:216893/148‑1 (MQ=255) | CCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAG‑CGTAATCTTTCGCACTGTTTT‑CATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAA > NC_000913/1540824‑1541342 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |