Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,684,016 A→G 5.7% S379G (AGC→GGC)  rstB → sensory histidine kinase of RstAB two‑component system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,684,0160AG 5.7% 511.2 / 12.7 153S379G (AGC→GGC) rstBsensory histidine kinase of RstAB two‑component system
Reads supporting (aligned to +/- strand):  ref base A (78/65);  new base G (3/6);  total (82/71)
Fisher's exact test for biased strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.79e-01

Only 100 of 153 total aligned reads displayed.

GTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTGGATAATTTGCTCAATAACGCCCTGCGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAGTATGTTGTAACTAAAGTGGT  >  NC_000913/1683767‑1684251
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                           
gTAACGCCCGAGAAAACGGTACGGATAAAAACGCTCGTGCAAGGCAATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTGGATAATTTGCTCAATAACGCCCTGCGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGAGACATTAATTGTTGAGGATGATGACCCAGGGATTGCCCCAGAAAACAGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCAAGACGGGATCGCTCAACCGGCGGCt                                                                                                                                                                                                                     <  2:592905/274‑1 (MQ=255)
                                                         ttGGATATGCGCTTAATGGAGCGCGTGCTGGATAATTTGCTCAATAACGCCCTGCGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCACCTCTATAGCACTGGCAATg                                                                                                                                                                         >  1:113050/1‑261 (MQ=255)
                                                                             gcgcGTGCTGGATAATTTGCTCAATAACGCCCTGCGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCActct                                                                                                                                                                                        >  2:126149/1‑226 (MQ=255)
                                                                             gcgcGTGCTGGATAATTTGCTCAATAACGCCCTGCGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCCCTTGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTg                                                                                                                                        >  2:487802/1‑274 (MQ=255)
                                                                                cgTGCTGGATAATTTGCTCAATAACGCCCTGCGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGa                                                                                                                                                     <  2:449953/258‑1 (MQ=255)
                                                                                 gTGCTGGATAATTTGCTCAATAACGCCCTGCGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAg                                                                                                                                                                                    <  4:126148/226‑1 (MQ=255)
                                                                                                           ccTGCGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCggg                                                                                                                                                                                                                                       >  7:585035/1‑149 (MQ=255)
                                                                                                             tGCGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTTTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCAGCTTCTCGTTTAGCTGGCCGtt                                                                                                        >  1:10860/1‑274 (MQ=255)
                                                                                                             tGCGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCTGTACGGTTAATTGTGACACCAGAGAACTGGGTGGGGCCCGCTTCCCGTTTAGCTGGCCGtt                                                                                                        >  2:282/1‑274 (MQ=255)
                                                                                                                ggTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCGGCTGGGATCga                                                                                                                                                                                                                                  <  7:148615/149‑2 (MQ=255)
                                                                                                                ggTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGc                                                                                                                                                                                                                                  <  8:480507/149‑1 (MQ=255)
                                                                                                                 gTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCGGCTGGGATCga                                                                                                                                                                                                                                  <  6:140192/148‑2 (MQ=255)
                                                                                                                 gTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCGGCTGGGATCga                                                                                                                                                                                                                                  <  5:95288/148‑2 (MQ=255)
                                                                                                                     tGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAAc                                                                                                                                                                                                                             >  8:458297/1‑149 (MQ=255)
                                                                                                                        cATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGGGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACcg                                                                                                                                                                                                                           <  5:132998/148‑1 (MQ=255)
                                                                                                                           tCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACcggcg                                                                                                                                                                                                                        >  5:240402/1‑148 (MQ=255)
                                                                                                                            cAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGc                                                                                                                                                                                                                   >  2:567395/1‑152 (MQ=255)
                                                                                                                              aCGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCt                                                                                                                                                                                                                     >  6:10851/1‑148 (MQ=255)
                                                                                                                              aCGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCt                                                                                                                                                                                                                     >  6:417725/1‑148 (MQ=255)
                                                                                                                               cGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTg                                                                                                                                                                                                                    >  6:387746/1‑148 (MQ=255)
                                                                                                                                ggTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCgggc                                                                                                                                                                                                               <  4:567393/152‑1 (MQ=255)
                                                                                                                                 gTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCgg                                                                                                                                                                                                                 >  8:272894/1‑149 (MQ=255)
                                                                                                                                  ttGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCggg                                                                                                                                                                                                                >  8:613747/1‑149 (MQ=255)
                                                                                                                                     aaaCCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCgggct                                                                                                                                                                                                              <  6:810178/148‑1 (MQ=255)
                                                                                                                                       aCCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCgggctggg                                                                                                                                                                                                           >  7:24360/1‑149 (MQ=255)
                                                                                                                                          aGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGAt                                                                                                                                                                                                                                     <  5:60365/120‑1 (MQ=255)
                                                                                                                                             cTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGggctggggctgg                                                                                                                                                                                                      >  6:185994/1‑148 (MQ=255)
                                                                                                                                               gCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCa                                                                                                                                                                                                    <  5:753340/148‑1 (MQ=255)
                                                                                                                                                  aCTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAAtt                                                                                                                                                                                                 >  5:297572/1‑148 (MQ=255)
                                                                                                                                                  aCTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTg                                                                                                                                                                                                <  7:489227/149‑1 (MQ=255)
                                                                                                                                                  aCTGTCGGGGAAGTGGGCAATATTAAGTGTGGCGGCTGTGGGCCCACGGATTACCCCAGAAAACCGCGAACAAATCTTGGAACCTTTTGTGCGCTTCGATCCCAGCCGGGAGCGCTCAACCGGCTGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTTCCCGCTTCTCGTTTAGCTTGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACt                                                                  <  4:642553/275‑1 (MQ=255)
                                                                                                                                                    tGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGt                                                                                                                                                                                               <  5:417725/148‑1 (MQ=255)
                                                                                                                                                    tGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTc                                                                                                                                                                                              <  7:458297/149‑1 (MQ=255)
                                                                                                                                                            aacagagCGAAATTAATTGTTTATGATGATGGCCCAGGGATTGCCCCAGACCACCGCGAACAGATCTTTGGGCCTTTTGTTCGCCTCGATCCCAGTCGTGATCGCTCTACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCtat                                                                                                                                                                                      <  8:758387/146‑1 (MQ=255)
                                                                                                                                                                gagCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAACCCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCTATTGTCCACTCTATATCa                                                                                                                                                                                  <  8:604937/149‑1 (MQ=255)
                                                                                                                                                                 agCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACGTATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCAc                                                                                                                                                                                 >  7:813768/1‑149 (MQ=255)
                                                                                                                                                                  gCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACt                                                                                                                                                                                >  8:242104/1‑149 (MQ=255)
                                                                                                                                                                   ggACATTAATTTTGTGGGATGATGGCCCATGGATGCCCCCAGAAAACCGCAAAAATATCTTTGACCCTTTGGTCCGCCGCGACCCCAGCCCGGGCGTCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGTGGTACGGTTAATTGTGCCACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCaa                                                 <  4:487800/274‑1 (MQ=255)
                                                                                                                                                                   cGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCaa                                                 >  2:706876/1‑275 (MQ=255)
                                                                                                                                                                     aCATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTgg                                                                                                                                                                              >  6:292064/1‑148 (MQ=255)
                                                                                                                                                                       aTTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCaa                                                                                                                                                                           >  7:701019/1‑149 (MQ=255)
                                                                                                                                                                           aTTGGTGAGGATGATGGCCCAGGGATTACCCCAGAAAACCGCGAACATATCTTTGAACCTTGTGGTCGGCTCGATCCCAACCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCACTTGTCCACTCGATAACACTGGCTATggg                                                                                                                                                                       >  8:101063/1‑149 (MQ=255)
                                                                                                                                                                            ttgttgAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGc                                                                                                                                                                      <  7:146985/149‑1 (MQ=255)
                                                                                                                                                                                tgAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCACGCTTCTCGTTTAGCTGGCCGTTATGGCCTAACATCCCGCACTTTACCCTTGCCTGACACTACGCGCACGATGGTCAAGTCACCCCGACtg                                    >  4:469411/1‑275 (MQ=255)
                                                                                                                                                                                 gAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCgggctg                                                                                                                                                                                                             >  2:41970/1‑105 (MQ=255)
                                                                                                                                                                                 gAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGac                                                                                                                                                    >  2:34035/1‑162 (MQ=255)
                                                                                                                                                                                   ggATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACgg                                                                                                                                                               >  8:277967/1‑149 (MQ=255)
                                                                                                                                                                                     atgatgGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCa                                                                                                                                                <  4:34034/162‑1 (MQ=255)
                                                                                                                                                                                       gatgGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCgggctggggc                                                                                                                                                                                                         >  4:426919/1‑103 (MQ=255)
                                                                                                                                                                                         tgGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAAtt                                                                                                                                                         <  7:774595/149‑1 (MQ=255)
                                                                                                                                                                                             ccAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATtgtg                                                                                                                                                      <  5:387746/148‑1 (MQ=255)
                                                                                                                                                                                             ccAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATtgtg                                                                                                                                                      <  5:174655/148‑1 (MQ=255)
                                                                                                                                                                                              cAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCgg                                                                                                                                                                                                                 >  4:788630/1‑88 (MQ=255)
                                                                                                                                                                                               aGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGac                                                                                                                                                    >  5:10069/1‑148 (MQ=255)
                                                                                                                                                                                                gggATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGaca                                                                                                                                                   >  5:463183/1‑148 (MQ=255)
                                                                                                                                                                                                gggATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGaca                                                                                                                                                   >  6:457281/1‑148 (MQ=255)
                                                                                                                                                                                                gggATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGaca                                                                                                                                                   >  6:581439/1‑148 (MQ=255)
                                                                                                                                                                                                gggATTGCCCAAGAAAACCGCGACCCTATCTGTGAACCTTTTGTTCGCGTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTAc                                                                                <  2:700093/215‑1 (MQ=255)
                                                                                                                                                                                                 ggATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCgggctggggctg                                                                                                                                                                                                       >  2:437626/1‑95 (MQ=255)
                                                                                                                                                                                                  gATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCgggctg                                                                                                                                                                                                             <  2:788632/88‑1 (MQ=255)
                                                                                                                                                                                                   attccccatgAAACCCGCAAACATACCTTTGAACCTTGGTTTGGACTCGTTCCCGGCCGGGAGCGCGTAACCGGGGGATGAGGGCTGGGGCTGGCAGTTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACCCCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAAGTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGAGCTATAAAATAAGTAtgt                 <  4:706874/266‑1 (MQ=255)
                                                                                                                                                                                                     tGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCaa                                                                                                                                                                                                   <  4:437624/95‑1 (MQ=255)
                                                                                                                                                                                                        cccAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAAc                                                                                                                                          >  8:718594/1‑149 (MQ=255)
                                                                                                                                                                                                          cAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACt                                                                                                                                         >  6:668118/1‑148 (MQ=255)
                                                                                                                                                                                                          cAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACct                                                                              <  2:487991/207‑1 (MQ=255)
                                                                                                                                                                                                             aaaaCCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAGCCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGggt                                                                                                                                     <  8:41385/149‑1 (MQ=255)
                                                                                                                                                                                                             aaaaCCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGggt                                                                                                                                     <  7:277967/149‑1 (MQ=255)
                                                                                                                                                                                                                  cgcgAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGTCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGACgg                                                     >  2:514552/1‑224 (MQ=255)
                                                                                                                                                                                                                  cgcgAACATATCTTTGAACCTTTTGTTCGCCGCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAGTATGTTGTAACTAAAGTGGt  <  2:282825/275‑1 (MQ=255)
                                                                                                                                                                                                                        cATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCtt                                                                                                                          <  7:242104/149‑1 (MQ=255)
                                                                                                                                                                                                                          tatCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTtct                                                                                                                        >  8:435624/1‑149 (MQ=255)
                                                                                                                                                                                                                           atCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTtctc                                                                                                                       >  8:849184/1‑149 (MQ=255)
                                                                                                                                                                                                                           atCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTtct                                                                                                                        >  5:124013/1‑148 (MQ=255)
                                                                                                                                                                                                                              tttGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGtt                                                                                                                    >  7:680847/1‑149 (MQ=255)
                                                                                                                                                                                                                               ttGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGttt                                                                                                                   <  7:762808/149‑1 (MQ=255)
                                                                                                                                                                                                                               ttGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTcac                                            <  2:95109/220‑1 (MQ=255)
                                                                                                                                                                                                                                 gAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAg                                                                                                                 >  7:148979/1‑149 (MQ=255)
                                                                                                                                                                                                                                 gAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAg                                                                                                                 >  8:7643/1‑149 (MQ=255)
                                                                                                                                                                                                                                  aaCCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTAc                                                                                                                                                                 >  1:22524/1‑100 (MQ=255)
                                                                                                                                                                                                                                  aaCCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCtt                                                                                                                          >  4:302986/1‑139 (MQ=255)
                                                                                                                                                                                                                                   aCCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAAt                         >  1:149027/1‑235 (MQ=255)
                                                                                                                                                                                                                                    ccTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCATTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTg                                                                                                              <  8:149911/149‑1 (MQ=255)
                                                                                                                                                                                                                                     cTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTgg                                                                                                             >  7:292695/1‑149 (MQ=255)
                                                                                                                                                                                                                                     cTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTgg                                                                                                             >  7:670542/1‑149 (MQ=255)
                                                                                                                                                                                                                                     cTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTgg                                                                                                             >  7:544372/1‑149 (MQ=255)
                                                                                                                                                                                                                                      ttttGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGtt                                                                                                                                                             <  3:22524/100‑1 (MQ=255)
                                                                                                                                                                                                                                      ttttGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTgg                                                                                                             >  6:559107/1‑148 (MQ=255)
                                                                                                                                                                                                                                       tttGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGc                                                                                                            <  6:4822/148‑1 (MQ=255)
                                                                                                                                                                                                                                       tttGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGAGCCCGCTTCTCGTTTAGCTGGc                                                                                                            >  5:759039/1‑148 (MQ=255)
                                                                                                                                                                                                                                           ttCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGtt                                                                                                        <  6:338086/148‑1 (MQ=255)
                                                                                                                                                                                                                                             cGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCGCTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTAt                                                                                                      <  6:240402/148‑1 (MQ=255)
                                                                                                                                                                                                                                             cGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATg                                                                                                     <  8:701019/149‑1 (MQ=255)
                                                                                                                                                                                                                                               ccTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGGAATGGGCGGTACGGTTAATTGTGACACCAGc                                                                                                                                              >  6:32433/1‑106 (MQ=255)
                                                                                                                                                                                                                                               ccTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGac                                                                                                                                                    >  1:194801/1‑100 (MQ=255)
                                                                                                                                                                                                                                               ccTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGc                                                                                                                                              <  5:32433/106‑1 (MQ=255)
                                                                                                                                                                                                                                               ccTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATgg                                                                                                    >  5:253772/1‑148 (MQ=255)
                                                                                                                                                                                                                                                 gcGATCCCGGCTGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCAt                                                                                                 >  8:237712/2‑149 (MQ=255)
                                                                                                                                                                                                                                                   gATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATaa                                                                                               >  7:714949/1‑149 (MQ=255)
                                                                                                                                                                                                                                                     tCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAAc                                                                                              <  5:185994/148‑1 (MQ=255)
                                                                                                                                                                                                                                                       ccAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACAt                                                                                            <  6:278774/148‑1 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                           
GTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTGGATAATTTGCTCAATAACGCCCTGCGGTACTGCCATTCAACGGTTGAAACCAGCCTGCTACTGTCGGGGAATAGAGCGACATTAATTGTTGAGGATGATGGCCCAGGGATTGCCCCAGAAAACCGCGAACATATCTTTGAACCTTTTGTTCGCCTCGATCCCAGCCGGGATCGCTCAACCGGCGGCTGCGGGCTGGGGCTGGCAATTGTCCACTCTATAGCACTGGCAATGGGCGGTACGGTTAATTGTGACACCAGCGAACTGGGTGGTGCCCGCTTCTCGTTTAGCTGGCCGTTATGGCATAACATCCCGCAATTTACCTCTGCCTGACACTACGCGCACGATGGTCAAGTCACCACGACTGTGCTATAAAATAAGTATGTTGTAACTAAAGTGGT  >  NC_000913/1683767‑1684251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: