Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,888,212 Δ1 bp 15.9% intergenic (+20/‑223) mnmE → / → tnaC tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase/tryptophanase leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,888,2120T.15.9% 135.7 / 21.3 44intergenic (+20/‑223)mnmE/tnaCtRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase/tryptophanase leader peptide
Reads supporting (aligned to +/- strand):  ref base T (12/25);  new base . (3/4);  total (15/29)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.65e-01

GGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGAT  >  NC_000913/3888069‑3888349
                                                                                                                                               |                                                                                                                                          
ggCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTg                                                                                                                                        <  2:786798/148‑1 (MQ=255)
ggCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGa                                                                                                                                       <  3:654781/149‑1 (MQ=255)
     gTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTc                                                                                                                                  <  3:787038/149‑1 (MQ=255)
       gAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAg                                                                                                                                <  3:586213/149‑1 (MQ=255)
              tgGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCA‑TCTGACAGTCAGAATGCGgc                                                                                                                        <  3:107136/149‑1 (MQ=255)
                gCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGgct                                                                                                                       >  2:301240/1‑148 (MQ=255)
                  ggAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGCATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCA‑TCTGACAGTCAGAATGCGgctcc                                                                                                                     <  3:747457/148‑3 (MQ=255)
                  ggAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGgcttcg                                                                                                                    >  3:446869/1‑149 (MQ=255)
                  ggAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCA‑TCTGACAGTCAGAATGCGgctcc                                                                                                                     >  4:747457/1‑146 (MQ=255)
                   gAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGAc                                                                                                                                      <  3:494920/131‑1 (MQ=255)
                   gAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGAc                                                                                                                                      >  4:494920/1‑131 (MQ=255)
                      gagTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGgcttcgcttc                                                                                                                >  3:403391/1‑149 (MQ=255)
                      gagTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGgcttcgcttc                                                                                                                <  3:699577/149‑1 (MQ=255)
                             gTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTAGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTa                                                                                                         <  3:289525/149‑1 (MQ=255)
                               cTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTAc                                                                                                        >  1:770807/1‑148 (MQ=255)
                                    acaGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTc                                                                                                   <  2:556704/148‑1 (MQ=255)
                                         agAACTTAAGCGAAATCACCGGGGAATTTACTTCAGCCGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTa                                                                                                         <  4:81264/137‑1 (MQ=255)
                                         agAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTa                                                                                                         >  3:81264/1‑137 (MQ=255)
                                            aCTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTAtt                                                                                           <  1:289474/148‑1 (MQ=255)
                                               tAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCA‑TCTGACAGTCAG‑aatg                                                                                                                           <  2:872652/112‑4 (MQ=255)
                                               tAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCA‑TCTGACAGTCAG‑aatg                                                                                                                           >  1:872652/1‑109 (MQ=255)
                                                  gCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAAc                                                                                    <  3:428262/149‑1 (MQ=255)
                                                   cGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAAc                                                                                    >  1:898433/1‑148 (MQ=255)
                                                        tCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCttt                                                                               <  2:770807/148‑1 (MQ=255)
                                                        tCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCttt                                                                               <  1:749890/148‑1 (MQ=255)
                                                           ccGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCttttttt                                                                           >  3:700488/1‑149 (MQ=255)
                                                              gggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaa                                                                         <  1:301240/148‑1 (MQ=255)
                                                                    ttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTa                                                                   <  2:779999/148‑1 (MQ=255)
                                                                        ttCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAAtt                                                              >  3:245155/1‑149 (MQ=255)
                                                                         ccAGACGACCTGCTGGGGCGGATTTTCTTCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACCTTAAAATTc                                                             <  4:779670/148‑1 (MQ=255)
                                                                                      tgGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTtat                                                <  4:446869/149‑1 (MQ=255)
                                                                                            ggATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCtt                                          <  4:403391/149‑1 (MQ=255)
                                                                                                tttCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaa                                       >  2:117227/1‑148 (MQ=255)
                                                                                                    tcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaaaaaaa                                  <  4:178024/149‑1 (MQ=255)
                                                                                                             tGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTaa                          <  2:898433/148‑1 (MQ=255)
                                                                                                              gTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAata                        <  4:700488/149‑1 (MQ=255)
                                                                                                              gTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAata                        <  4:847879/149‑1 (MQ=255)
                                                                                                                 ttGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTg                     <  3:648139/149‑1 (MQ=255)
                                                                                                                     taagtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCTTTTTTTTAAACATTaaa                                                                 <  2:669369/101‑1 (MQ=255)
                                                                                                                     taagtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCTTTTTTTTAAACATTaaa                                                                 >  1:669369/1‑101 (MQ=255)
                                                                                                                                    aCGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAAc           >  3:879317/1‑140 (MQ=255)
                                                                                                                                    aCGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAAc           <  4:879317/140‑1 (MQ=255)
                                                                                                                                         gCCGCA‑TCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgat  >  2:765557/1‑144 (MQ=255)
                                                                                                                                         gCCGCA‑TCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTgat  <  1:765557/144‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                          
GGCAGGTGAACTGCTGGCGGAAGAGTTGCGTCTGGCACAGCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAG‑ATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTTAATATTGCTCCCCGAACGATTGTGAT  >  NC_000913/3888069‑3888349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑