Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,400,355:1 +A 5.1% coding (1201/1338 nt) nuoF ← NADH:ubiquinone oxidoreductase, chain F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,400,3551.A 5.1% 330.2 / 12.7 78coding (1201/1338 nt)nuoFNADH:ubiquinone oxidoreductase, chain F
Reads supporting (aligned to +/- strand):  ref base . (34/40);  new base A (2/2);  total (36/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.78e-01

TCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCAC  >  NC_000913/2400207‑2400488
                                                                                                                                                     |                                                                                                                                     
tCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGc                                                                                                                                        >  4:221575/1‑149 (MQ=255)
 cGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGc                                                                                                                                        >  2:812773/1‑148 (MQ=255)
    aaTTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTg                                                                                                                                    <  1:783742/148‑1 (MQ=255)
      ttCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGc                                                                                                                                 <  3:221575/149‑1 (MQ=255)
       tCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGca                                                                                                                                <  4:393710/149‑1 (MQ=255)
              ccAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGaaa                                                                                                                          <  2:681806/148‑1 (MQ=255)
              ccAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATAAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCCCTGCACCAGGTGCGTGGGCACAGAAAg                                                                                                                         <  4:532031/149‑1 (MQ=255)
              ccAGCGCTCTTTCAGCAGGATCGGCTGAATCCCATTAATCAACTGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCCCCAGGTGCGTGGGCACAGAAAg                                                                                                                         >  3:532031/1‑149 (MQ=255)
               cAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAg                                                                                                                         <  2:415023/148‑1 (MQ=255)
                aGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGtt                                                                                                                       <  3:741631/149‑1 (MQ=255)
                aGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGtt                                                                                                                       >  3:598180/1‑149 (MQ=255)
                 gcgcTCTTGCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGttt                                                                                                                      <  3:661679/149‑1 (MQ=255)
                     tctTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTAcc                                                                                                                  >  4:901014/1‑149 (MQ=255)
                        ttCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCg                                                                                                                >  1:723058/1‑148 (MQ=255)
                         tCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCggg                                                                                                              >  3:435531/1‑149 (MQ=255)
                          cagcagGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCggg                                                                                                              <  2:525008/148‑1 (MQ=255)
                          cagcagGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGc                                                                                                             <  3:851527/149‑1 (MQ=255)
                            gcagGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCt                                                                                                           >  3:838618/1‑149 (MQ=255)
                              agGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTa                                                                                                          <  1:794766/148‑1 (MQ=255)
                                     gCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCg                                                                                                   <  2:519317/148‑1 (MQ=255)
                                       tGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGaca                                                                                                >  4:39118/1‑149 (MQ=255)
                                       tGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGac                                                                                                 <  1:675680/148‑1 (MQ=255)
                                        gAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGacac                                                                                               <  4:435531/149‑1 (MQ=255)
                                         aaTCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGAcaca                                                                                              <  4:838618/149‑1 (MQ=255)
                                           tCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGt                                                                                            >  3:148817/1‑149 (MQ=255)
                                             ccATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGac                                                                                                 >  3:53667/1‑142 (MQ=255)
                                             ccATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGac                                                                                                 <  4:53667/142‑1 (MQ=255)
                                             ccATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTg                                                                                          >  3:531977/1‑149 (MQ=255)
                                              cATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTg                                                                                          >  2:777397/1‑148 (MQ=255)
                                               aTTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCt                                                                                        >  3:183919/1‑149 (MQ=255)
                                                ttAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCt                                                                                        <  2:529174/148‑1 (MQ=255)
                                                         tGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTc                                                                              <  4:224237/149‑1 (MQ=255)
                                                          gggTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTc                                                                              >  2:561721/1‑148 (MQ=255)
                                                          gggTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAAACGACACAGTTGCTCAAGTGTTTCg                                                                             <  4:9125/149‑1 (MQ=255)
                                                                    gAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGca                                                                                                                                <  4:503015/88‑1 (MQ=255)
                                                                    gAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGca                                                                                                                                >  3:503015/1‑88 (MQ=255)
                                                                      aCGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGc                                                                 >  3:406433/1‑149 (MQ=255)
                                                                         gCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTg                                                               >  1:565108/1‑148 (MQ=255)
                                                                         gCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCCAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTg                                                               <  1:715902/148‑1 (MQ=255)
                                                                          cTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGa                                                              <  1:750137/148‑1 (MQ=255)
                                                                          cTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGa                                                              <  1:345621/148‑1 (MQ=255)
                                                                          cTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGa                                                              >  2:345621/1‑148 (MQ=255)
                                                                           tGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGAcc                                                            <  3:901014/149‑1 (MQ=255)
                                                                            gTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGa                                                              <  2:233212/146‑1 (MQ=255)
                                                                            gTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGa                                                              >  1:233212/1‑146 (MQ=255)
                                                                            gTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGAcc                                                            >  2:324447/1‑148 (MQ=255)
                                                                              ttGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCtt                                                          >  1:46781/1‑148 (MQ=255)
                                                                                gATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTaa                                                                                                         >  4:69569/1‑99 (MQ=255)
                                                                                gATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTaa                                                                                                         <  4:730694/99‑1 (MQ=255)
                                                                                gATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTaa                                                                                                         >  3:730694/1‑99 (MQ=255)
                                                                                gATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCGCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTaa                                                                                                         <  3:69569/99‑1 (MQ=255)
                                                                                   tCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTcacca                                                     >  1:261773/1‑148 (MQ=255)
                                                                                   tCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTcacca                                                     >  1:895632/1‑148 (MQ=255)
                                                                                    cccGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTcaccac                                                    >  2:857720/1‑148 (MQ=255)
                                                                                     ccGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCAGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACg                                                   >  3:260941/1‑149 (MQ=255)
                                                                                      cGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGc                                                  >  2:335497/1‑148 (MQ=255)
                                                                                               ttcttcGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCAGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGcgca                                         <  4:260941/149‑1 (MQ=255)
                                                                                                  ttcGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGcgcacgca                                     <  4:193257/149‑1 (MQ=255)
                                                                                                  ttcGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGcgcacgc                                      <  1:812773/148‑1 (MQ=255)
                                                                                                     gcgGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGa                                   <  1:698437/148‑1 (MQ=255)
                                                                                                     gcgGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGa                                   <  1:335497/148‑1 (MQ=255)
                                                                                                      cgGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGaa                                  <  2:46781/148‑1 (MQ=255)
                                                                                                          aaTATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCTGAATTTTc                             <  3:139267/149‑1 (MQ=255)
                                                                                                              tttGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACg                          <  1:506569/148‑1 (MQ=255)
                                                                                                              tttGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACg                          <  2:895632/148‑1 (MQ=255)
                                                                                                               ttatggtcgcTCTGAAACGCCTCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTc                       <  4:637347/142‑1 (MQ=255)
                                                                                                                 gATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTcc                      >  3:5783/1‑149 (MQ=255)
                                                                                                                 gATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTc                       <  2:565108/148‑1 (MQ=255)
                                                                                                                   tGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGaa                                                                                                                           <  1:443280/46‑1 (MQ=255)
                                                                                                                   tGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGaa                                                                                                                           >  2:443280/1‑46 (MQ=255)
                                                                                                                      cgcTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGc                 >  3:789769/1‑149 (MQ=255)
                                                                                                                                 ggCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTcgcg      <  4:503291/149‑1 (MQ=255)
                                                                                                                                  gCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTcgcg      >  1:2631/1‑148 (MQ=255)
                                                                                                                                   cTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCgg     >  2:161448/1‑148 (MQ=255)
                                                                                                                                    tCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCa   >  3:226916/1‑149 (MQ=255)
                                                                                                                                     cccacgCACCTGGTGCAGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTc                       >  4:399937/6‑129 (MQ=255)
                                                                                                                                     cccacgCACCTGGTGCAGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTc                       <  3:399937/124‑1 (MQ=255)
                                                                                                                                     ccACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTcgc       <  4:774660/144‑1 (MQ=255)
                                                                                                                                     ccACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTcgc       >  3:774660/1‑144 (MQ=255)
                                                                                                                                     ccACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCAc  >  3:866811/1‑149 (MQ=255)
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TCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCCACTGCACCAGGTGCGTGGGCACAGAAAGTTTTACCCGGGCCTAAGAATCGACACAGTTGCTCAAGTGTTTCGATATCGCCCGGCTGACCTTCACCACGCTCCAGCGCACGCAGAATTTTCACGCTCCACGGCAGACCGTCGCGGCAC  >  NC_000913/2400207‑2400488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: