Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 752,067 C→A 6.2% D35Y (GAC→TAC) ‡ ybgQ ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913752,0670CA 6.2% 287.8 / 6.5 81D35Y (GAC→TAC) ‡ybgQputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (33/43);  new base A (2/3);  total (35/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

ATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGC  >  NC_000913/751926‑752214
                                                                                                                                             |                                                                                                                                                   
aTGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCAtt                                                                                                                                              <  3:454391/149‑1 (MQ=255)
aTGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCAt                                                                                                                                               <  2:149655/148‑1 (MQ=255)
aTGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCAt                                                                                                                                               <  2:724315/148‑1 (MQ=255)
 tGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCAt                                                                                                                                               <  3:134571/147‑1 (MQ=255)
 tGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCAt                                                                                                                                               >  4:134571/1‑147 (MQ=255)
  ggAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCAtt                                                                                                                                              <  4:606620/147‑1 (MQ=255)
  ggAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCAtt                                                                                                                                              >  3:606620/1‑147 (MQ=255)
   gAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGa                                                                                                                                            <  2:2522/148‑1 (MQ=255)
   gAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGCGACCGCATTGa                                                                                                                                            <  1:309971/148‑1 (MQ=255)
    aaTGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGCGACCGCATTGAta                                                                                                                                          <  4:259838/149‑2 (MQ=255)
       ggTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTa                                                                                                                                        <  2:444921/148‑1 (MQ=255)
        gTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATc                                                                                                                                      <  3:723785/149‑1 (MQ=255)
        gTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATc                                                                                                                                      >  4:672983/1‑149 (MQ=255)
         ttttGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCg                                                                                                                                     >  3:17063/1‑149 (MQ=255)
                 cccTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACAt                                                                                                                              >  1:397588/1‑148 (MQ=255)
                 cccTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACAt                                                                                                                              >  2:417252/1‑148 (MQ=255)
                   cTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTc                                                                                                                            >  1:55791/1‑148 (MQ=255)
                   cTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCa                                                                                                                           >  4:505083/1‑149 (MQ=255)
                    tttttttGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAg                                                                                                                          >  4:641134/1‑149 (MQ=255)
                    tttttttGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGCGACCGCATTGa                                                                                                                                            >  1:672970/1‑131 (MQ=255)
                     ttttttGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGCGACCGCATTGAta                                                                                                                                          >  2:278602/1‑131 (MQ=255)
                     ttttttGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGCGACCGCATTGAta                                                                                                                                          <  1:278602/132‑2 (MQ=255)
                      tttttGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGa                                                                                                                         <  2:290162/148‑1 (MQ=255)
                           gCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAAt                                                                                                                   <  4:892497/149‑1 (MQ=255)
                           gCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAAt                                                                                                                   <  4:178779/149‑1 (MQ=255)
                            ccAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAAt                                                                                                                   >  2:768513/1‑148 (MQ=255)
                             cAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTc                                                                                                                 <  4:17063/149‑1 (MQ=255)
                              aGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTc                                                                                                                 <  2:397588/148‑1 (MQ=255)
                                ttAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCga                                                                                                                                    >  4:540200/1‑127 (MQ=255)
                                ttAATTTTGTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCga                                                                                                                                    <  3:540200/127‑1 (MQ=255)
                                       tttGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCAt                                                                                                        <  1:439583/148‑1 (MQ=255)
                                       tttGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCAt                                                                                                        <  1:417252/148‑1 (MQ=255)
                                               ttACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAgg                                                                                               >  3:105386/1‑149 (MQ=255)
                                                   tGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCCTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCAt                                                                                            >  1:81360/1‑148 (MQ=255)
                                                    gATTTTGTGGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATACAGATTGAATTCGACCGCCATTGCGCAAGGCATc                                                                                           >  1:783329/1‑148 (MQ=255)
                                                       tttGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCaa                                                                                                 <  3:25449/139‑1 (MQ=255)
                                                       tttGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCaa                                                                                                 >  4:25449/1‑139 (MQ=255)
                                                       tttGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGcc                                                                                        >  1:879458/1‑148 (MQ=255)
                                                       tttGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGcc                                                                                        <  1:764922/148‑1 (MQ=255)
                                                        ttgttgTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCa                                                                                       <  1:768513/148‑1 (MQ=255)
                                                          gttgttATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATg                                                                                     <  2:857550/148‑1 (MQ=255)
                                                           ttgttATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGa                                                                                    <  1:664055/148‑1 (MQ=255)
                                                           ttgttATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGa                                                                                    >  1:569338/1‑148 (MQ=255)
                                                                  tCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACcaggca                                                                             >  1:375098/1‑148 (MQ=255)
                                                                   cACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACcaggcag                                                                            <  2:879458/148‑1 (MQ=255)
                                                                     ccGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGa                                                                          >  1:76290/1‑148 (MQ=255)
                                                                         aaCGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACaa                                                                     >  4:699703/1‑149 (MQ=255)
                                                                           cGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACaaaa                                                                   <  3:505083/149‑1 (MQ=255)
                                                                               aaCAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAagag                                                                >  2:310654/1‑148 (MQ=255)
                                                                               aaCAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAagag                                                                >  2:499551/1‑148 (MQ=255)
                                                                                aCAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAagaga                                                               <  2:81360/148‑1 (MQ=255)
                                                                                 cAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGt                                                             <  3:789551/149‑1 (MQ=255)
                                                                                  aaaaTATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGt                                                             <  2:76290/148‑1 (MQ=255)
                                                                                  aaaaTATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGt                                                             <  2:569338/148‑1 (MQ=255)
                                                                                        ttCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATaaa                                                      >  3:844791/1‑149 (MQ=255)
                                                                                              gggAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTc                                                 <  1:465269/148‑1 (MQ=255)
                                                                                               ggAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCa                                                <  1:310654/148‑1 (MQ=255)
                                                                                               ggAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCAc                                               <  3:672983/149‑1 (MQ=255)
                                                                                                   cAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAata                                                    >  3:804820/1‑140 (MQ=255)
                                                                                                   cAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAata                                                    <  4:804820/140‑1 (MQ=255)
                                                                                                       gACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTcc                                        >  1:420289/1‑148 (MQ=255)
                                                                                                        aCTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACAGTGTCCAt                                      <  3:835042/149‑1 (MQ=255)
                                                                                                              ttttCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTg                                 <  1:499551/148‑1 (MQ=255)
                                                                                                              ttttCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGa                                <  3:641134/149‑1 (MQ=255)
                                                                                                               tttCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGa                                <  2:375098/148‑1 (MQ=255)
                                                                                                                    tttAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATAcc                                   >  1:65841/1‑140 (MQ=255)
                                                                                                                    tttAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATAcc                                   <  2:65841/140‑1 (MQ=255)
                                                                                                                    tttAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAata                          >  4:527493/1‑149 (MQ=255)
                                                                                                                    tttAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAata                          >  3:277642/1‑149 (MQ=255)
                                                                                                                       aataatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATAttt                       <  3:699703/149‑1 (MQ=255)
                                                                                                                          aatGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTAt                    >  3:594537/1‑149 (MQ=255)
                                                                                                                            tGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATCGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGa                  >  4:617833/1‑149 (MQ=255)
                                                                                                                                atatCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATaaa                                                      <  2:146374/109‑1 (MQ=255)
                                                                                                                                atatCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATaaa                                                      >  1:146374/1‑109 (MQ=255)
                                                                                                                                atatCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAgg              <  4:844791/149‑1 (MQ=255)
                                                                                                                                 tatCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAATAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAgg              <  2:783329/148‑1 (MQ=255)
                                                                                                                                     aaTGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGgcggcg         >  3:151398/1‑149 (MQ=255)
                                                                                                                                       tGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCgat       <  4:753577/149‑1 (MQ=255)
                                                                                                                                            tCGCGCATTGATTTATCGAGAACATTCAGATTGAAt                                                                                                                   >  4:306763/1‑36 (MQ=255)
                                                                                                                                            tCGCGCATTGATTTATCGAGAACATTCAGATTGAAt                                                                                                                   <  3:306763/36‑1 (MQ=255)
                                                                                                                                            tCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATgc  <  4:105386/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
ATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGAGCAATGACTCCTTTTTCCTTTAATAATGAAATATCAATGCGGTCGCGCATTGATTTATCGAGAACATTCAGATTGAATTCGACCGCCATTGCGCAAGGCATCGCCATGACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGC  >  NC_000913/751926‑752214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: