Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 762,869 A→T 6.3% intergenic (+130/‑145) sucB → / → sucC dihydrolipoyltranssuccinase/succinyl‑CoA synthetase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913762,8690AT 6.3% 455.8 / 14.9 127intergenic (+130/‑145)sucB/sucCdihydrolipoyltranssuccinase/succinyl‑CoA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (60/59);  new base T (4/4);  total (64/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

Only 100 of 129 total aligned reads displayed.

TCTGCTGCTGGACGTGTAGTAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACA  >  NC_000913/762721‑763014
                                                                                                                                                    |                                                                                                                                                 
tCTGCTGCTGGACGTGTAGTAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCa                                                                                                                                                   >  3:243150/1‑149 (MQ=255)
          gACGTGTAGTAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATGCGGCGCTTGcgcctta                                                                                                                                          <  1:667948/148‑4 (MQ=38)
                 agtagtTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACaa                                                                                                                                  <  4:469382/149‑1 (MQ=255)
                 agtagtTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACaa                                                                                                                                  >  3:256179/1‑149 (MQ=255)
                  gtagtTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAg                                                                                                                                 >  3:569339/1‑149 (MQ=255)
                  gtagtTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAg                                                                                                                                 >  3:429916/1‑149 (MQ=255)
                   tagtTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGGCGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAg                                                                                                                                 >  2:583033/1‑148 (MQ=255)
                      tttAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGAt                                                                                                                             >  4:727239/1‑149 (MQ=255)
                       ttAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGAt                                                                                                                             >  2:403180/1‑148 (MQ=255)
                       ttAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGAt                                                                                                                             >  1:375158/1‑148 (MQ=255)
                       ttAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGAt                                                                                                                             >  1:701242/1‑148 (MQ=255)
                       ttAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATg                                                                                                                            <  4:429916/149‑1 (MQ=255)
                         aaGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATGGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATACGGCGCAAGCGCCGCATCCGACAAGCGATGcc                                                                                                                          <  4:553442/149‑1 (MQ=255)
                            tttCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATGCGGCGCTTGCGCCTTATCCGAc                                                                                                                                    <  3:40804/136‑1 (MQ=37)
                            tttCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATGCGGCGCTTGCGCCTTATCCGAc                                                                                                                                    >  4:40804/1‑136 (MQ=37)
                            tttCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTg                                                                                                                        >  2:421154/1‑148 (MQ=255)
                             ttCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGa                                                                                                                       <  1:723468/148‑1 (MQ=255)
                                   tGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACg                                                                                                                >  4:868879/1‑149 (MQ=255)
                                      aCTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGtt                                                                                                              <  1:460846/148‑1 (MQ=255)
                                       cTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGttt                                                                                                             >  2:260968/1‑148 (MQ=255)
                                         gTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTaaa                                                                                                           >  1:158772/1‑148 (MQ=255)
                                           aGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACgt                                                                                                        <  3:868879/149‑1 (MQ=255)
                                             aCCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACgtgt                                                                                                      >  3:321483/1‑149 (MQ=255)
                                                 gATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTa                                                                                                  >  3:303871/1‑149 (MQ=255)
                                                  aTAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGAtg                                                                                                                     >  1:682823/1‑129 (MQ=255)
                                                  aTAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGAtg                                                                                                                     <  2:682823/129‑1 (MQ=255)
                                                       gCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATGCGGCGCTTGcgccttat                                                                                                                                         >  2:781646/1‑100 (MQ=25)
                                                       gCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGAt                                                                                                                             <  4:262201/116‑1 (MQ=255)
                                                       gCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGAt                                                                                                                             >  3:262201/1‑116 (MQ=255)
                                                       gCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAgg                                                                                             <  2:701242/148‑1 (MQ=255)
                                                       gCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGc                                                                                            >  4:284993/1‑149 (MQ=255)
                                                       gCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGc                                                                                            >  4:74959/1‑149 (MQ=255)
                                                         aTTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAAc                                                                                                          <  4:679060/133‑1 (MQ=255)
                                                         aTTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAAc                                                                                                          >  3:679060/1‑133 (MQ=255)
                                                          ttATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTa                                                                                         >  4:711227/1‑149 (MQ=255)
                                                           tATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTa                                                                                                            >  2:430969/1‑129 (MQ=255)
                                                           tATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTa                                                                                                            <  1:430969/129‑1 (MQ=255)
                                                              cGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGAtg                                                                                                                     >  1:785317/1‑117 (MQ=255)
                                                              cGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGAtg                                                                                                                     <  2:785317/117‑1 (MQ=255)
                                                                 cTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTg                                                                                   >  1:645150/1‑148 (MQ=255)
                                                                  ttCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGa                                                                                  <  2:100774/148‑1 (MQ=255)
                                                                       cGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGa                                                                                                                  <  3:275635/111‑1 (MQ=255)
                                                                       cGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGa                                                                                                                  >  4:275635/1‑111 (MQ=255)
                                                                       cGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACa                                                                            >  4:406839/1‑149 (MQ=255)
                                                                        ggCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACaa                                                                           <  3:284993/149‑1 (MQ=255)
                                                                        ggCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACa                                                                            <  1:403180/148‑1 (MQ=255)
                                                                           aaTTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGc                                                                        >  4:610593/1‑149 (MQ=255)
                                                                            aTTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGttt                                                                                                             >  3:837567/1‑111 (MQ=255)
                                                                            aTTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGttt                                                                                                             <  4:837567/111‑1 (MQ=255)
                                                                              tGAAGCCTGATGCGGCGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGccc                                                                      <  1:583033/148‑1 (MQ=255)
                                                                              tGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCg                                                                     <  4:237053/149‑1 (MQ=255)
                                                                                     tGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAgcg                                                                                                                                               >  2:746727/1‑68 (MQ=255)
                                                                                     tGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAgcg                                                                                                                                               <  1:746727/68‑1 (MQ=255)
                                                                                     tGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGa                                                              <  3:727239/149‑1 (MQ=255)
                                                                                       aTGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATa                                                            <  3:463919/149‑1 (MQ=255)
                                                                                       aTGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATa                                                            <  4:700882/149‑1 (MQ=255)
                                                                                            aCGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGaaa                                                       <  4:256179/149‑1 (MQ=255)
                                                                                            aCGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGaaa                                                       <  4:367462/149‑1 (MQ=255)
                                                                                              gCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAat                                                      <  2:201841/148‑1 (MQ=255)
                                                                                                 gACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTc                                                  <  4:569339/149‑1 (MQ=255)
                                                                                                  aCGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTc                                                  <  1:358226/148‑1 (MQ=255)
                                                                                                  aCGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCg                                                 >  3:104081/1‑149 (MQ=255)
                                                                                                  aCGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCg                                                 >  4:816354/1‑149 (MQ=255)
                                                                                                  aCGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCg                                                 <  3:74959/149‑1 (MQ=255)
                                                                                                        cTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTa                                            >  1:121222/1‑148 (MQ=255)
                                                                                                          tATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATAtt                                                   >  1:68142/1‑139 (MQ=255)
                                                                                                          tATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATAtt                                                   <  2:68142/139‑1 (MQ=255)
                                                                                                             cAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGGGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGttt                                      <  4:540279/149‑1 (MQ=255)
                                                                                                               ggCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTaa                                    >  4:681872/1‑149 (MQ=255)
                                                                                                                 ccTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTaaa                                   >  2:31885/1‑148 (MQ=255)
                                                                                                                      gggACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATaa                             <  3:711227/149‑1 (MQ=255)
                                                                                                                        gACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACg                           >  3:588348/1‑149 (MQ=255)
                                                                                                                         accaccAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACg                           >  1:374882/1‑148 (MQ=255)
                                                                                                                          ccaccaATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGa                          >  1:284845/1‑148 (MQ=255)
                                                                                                                           caccaATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGAtt                        >  3:805684/1‑149 (MQ=255)
                                                                                                                             ccaATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTa                       >  1:118323/1‑148 (MQ=255)
                                                                                                                             ccaATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTa                       <  1:453359/148‑1 (MQ=255)
                                                                                                                             ccaATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTa                       <  2:375158/148‑1 (MQ=255)
                                                                                                                              caATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATAtt                                                   >  1:227525/1‑119 (MQ=255)
                                                                                                                                 tGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGa                   <  1:236545/148‑1 (MQ=255)
                                                                                                                                 tGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGa                   >  2:236545/1‑148 (MQ=255)
                                                                                                                                     ggTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCg                                                 >  4:341473/1‑114 (MQ=255)
                                                                                                                                      gTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGAt              <  1:31885/148‑1 (MQ=255)
                                                                                                                                      gTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTAAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGAt              <  2:158772/148‑1 (MQ=255)
                                                                                                                                       tCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCgg                                                <  1:277651/113‑1 (MQ=255)
                                                                                                                                       tCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCgg                                                >  2:277651/1‑113 (MQ=255)
                                                                                                                                       tCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATgg            <  3:406839/149‑1 (MQ=255)
                                                                                                                                       tCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATgg            >  4:425286/1‑149 (MQ=255)
                                                                                                                                       tCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATg             >  2:877813/1‑148 (MQ=255)
                                                                                                                                        cGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGa           <  4:284299/149‑1 (MQ=255)
                                                                                                                                          gATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACa         <  4:345702/149‑1 (MQ=255)
                                                                                                                                           aTAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACa         <  1:530795/148‑1 (MQ=255)
                                                                                                                                           aTAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACa         >  2:725355/1‑148 (MQ=255)
                                                                                                                                           aTAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAg        >  4:413676/1‑149 (MQ=255)
                                                                                                                                             aaGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGa       <  2:860558/148‑1 (MQ=255)
                                                                                                                                                gcgcAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAaca    <  2:645150/148‑1 (MQ=255)
                                                                                                                                                 cgcAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAacac   >  1:227623/1‑148 (MQ=255)
                                                                                                                                                  gcAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGACTACTGAAGGATGGACAGAAcaca  >  1:638629/1‑148 (MQ=255)
                                                                                                                                                   cAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCg                                                 >  2:115509/1‑100 (MQ=255)
                                                                                                                                                   cAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCg                                                 <  1:115509/100‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TCTGCTGCTGGACGTGTAGTAGTTTAAGTTTCACCTGCACTGTAGACCGGATAAGGCATTATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTACGGGTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACA  >  NC_000913/762721‑763014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: