Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 783,000 C→A 6.6% Q306K (CAG→AAG)  nadA → quinolinate synthase, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913783,0000CA 6.6% 471.3 / 15.6 134Q306K (CAG→AAG) nadAquinolinate synthase, subunit A
Reads supporting (aligned to +/- strand):  ref base C (54/71);  new base A (3/6);  total (57/77)
Fisher's exact test for biased strand distribution p-value = 7.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01

Only 100 of 134 total aligned reads displayed.

GGCTTATTGTGGCAACCGATCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGTATGCTGGATTTTGCGGCTACACTACGTGGATAACGAATAATAAGGCGTAAC  >  NC_000913/782854‑783146
                                                                                                                                                  |                                                                                                                                                  
ggCTTATTGTGGCAACCGATCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCa                                                                                                                                                   <  1:568824/148‑1 (MQ=255)
ggCTTATTGTGGCAACCGATCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAg                                                                                                                                                  <  3:898266/149‑1 (MQ=255)
ggCTTATTGTGGCAACCGATCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAg                                                                                                                                                  <  4:588860/149‑1 (MQ=255)
 gCTTATTGTGGCAACCGATCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAg                                                                                                                                                  <  2:366232/148‑1 (MQ=255)
   ttATTGTGGCAACCGATCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGcc                                                                                                                                               >  3:142997/1‑149 (MQ=255)
      ttGTGGCAACCGATCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATc                                                                                                                                            <  3:256314/149‑1 (MQ=255)
      ttGTGGCAACCGATCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATc                                                                                                                                            <  3:821771/149‑1 (MQ=255)
       tgtgGCAACCGATCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATc                                                                                                                                            >  1:184209/1‑148 (MQ=255)
         tgGCAACCGATCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTGAAGGCCAttcat                                                                                                                                         <  3:396366/149‑5 (MQ=255)
          ggCAACCGATCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCag                                                                                                                                        >  3:489162/1‑149 (MQ=255)
          ggCAACCGATCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTGAAGGCCAttaat                                                                                                                                         <  1:94274/148‑5 (MQ=255)
           gCAACCGATCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCaga                                                                                                                                       <  4:799800/149‑1 (MQ=255)
           gCAACCGATCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCag                                                                                                                                        <  1:336285/148‑1 (MQ=255)
           gCAACCGATCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCag                                                                                                                                        <  2:29841/148‑1 (MQ=255)
            cAACCGATCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCaga                                                                                                                                       >  1:113670/1‑148 (MQ=255)
            cAACCGATCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCaga                                                                                                                                       >  2:13060/1‑148 (MQ=255)
             aaCCGATCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAgg                                                                                                                                     >  3:807434/1‑149 (MQ=255)
               ccGATCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCa                                                                                                                                   <  3:345277/149‑1 (MQ=255)
                cGATCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCAt                                                                                                                                  <  3:428945/149‑1 (MQ=255)
                  aTCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCAtt                                                                                                                                 <  2:623945/148‑1 (MQ=255)
                  aTCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTa                                                                                                                                <  4:414712/149‑1 (MQ=255)
                       ggTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAAc                                                                                                                            >  2:846072/1‑148 (MQ=255)
                       ggTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACa                                                                                                                           <  4:489162/149‑1 (MQ=255)
                        gTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACa                                                                                                                           >  2:344167/1‑148 (MQ=255)
                        gTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACa                                                                                                                           <  1:440778/148‑1 (MQ=255)
                          aTTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACagga                                                                                                                        <  4:706737/149‑1 (MQ=255)
                          aTTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACagga                                                                                                                        >  3:688266/1‑149 (MQ=255)
                           ttttCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACaggaa                                                                                                                       <  3:462211/149‑1 (MQ=255)
                           ttttCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTGAAGGCCAt                                                                                                                                             >  4:387342/1‑127 (MQ=255)
                           ttttCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTGAAGGCCAt                                                                                                                                             <  3:387342/127‑1 (MQ=255)
                            tttCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACaggaa                                                                                                                       <  1:398376/148‑1 (MQ=255)
                            tttCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACaggaa                                                                                                                       >  2:534017/1‑148 (MQ=255)
                             ttCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACaggaagg                                                                                                                     <  4:547831/149‑1 (MQ=255)
                              tCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACaggaagg                                                                                                                     <  1:344167/148‑1 (MQ=255)
                               cTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACaggaagga                                                                                                                    <  1:764852/148‑1 (MQ=255)
                                tACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTGAAGGCCAttcat                                                                                                                                         >  3:515031/1‑122 (MQ=255)
                                 aCAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGgaaggaag                                                                                                                  >  1:659093/1‑148 (MQ=255)
                                 aCAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGgaaggaag                                                                                                                  >  2:348335/1‑148 (MQ=255)
                                    aaaTGCGGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTGAAGGCCAttca                                                                                                                                          >  2:496101/1‑118 (MQ=255)
                                    aaaTGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTGAAGGCCAttca                                                                                                                                          <  1:496101/121‑4 (MQ=255)
                                     aaTGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAAt                                                                                                              >  2:771387/1‑148 (MQ=255)
                                     aaTGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATc                                                                                                             <  4:142997/149‑1 (MQ=255)
                                     aaTGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTCGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGGAGGAAGCCAt                                                                                                              <  1:742852/148‑1 (MQ=255)
                                          agcagGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACaggaa                                                                                                                       >  4:647268/1‑134 (MQ=255)
                                          agcagGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACaggaa                                                                                                                       <  3:647268/134‑1 (MQ=255)
                                            cagGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAgg                                                                                                       <  1:641865/148‑1 (MQ=255)
                                            cagGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAgg                                                                                                       <  2:184209/148‑1 (MQ=255)
                                              ggCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACaggaagga                                                                                                                    <  1:145030/133‑1 (MQ=255)
                                              ggCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACaggaagga                                                                                                                    >  2:145030/1‑133 (MQ=255)
                                                cGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCa                                                                                                   >  1:900628/1‑148 (MQ=255)
                                                cGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCa                                                                                                   <  1:846072/148‑1 (MQ=255)
                                                     ccAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTtga                                                                                             <  4:343866/149‑1 (MQ=255)
                                                      cAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTtga                                                                                             >  1:791320/1‑148 (MQ=255)
                                                      cAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTtga                                                                                             <  2:277360/148‑1 (MQ=255)
                                                          tAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGaaa                                                                                        <  3:46530/149‑1 (MQ=255)
                                                               agTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTg                                                                                   >  3:320779/1‑149 (MQ=255)
                                                                gTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATCGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTg                                                                                   <  1:229187/148‑1 (MQ=255)
                                                                  tACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCg                                                                                 >  1:267269/1‑148 (MQ=255)
                                                                  tACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGAGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGCCATTAGAACAGGAAGGAAGCAATCACGAGGTTCTTGTTGATTAAAGGCTGCg                                                                                 <  2:659093/148‑1 (MQ=255)
                                                                      ggAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCgagaga                                                                            <  4:524375/149‑1 (MQ=255)
                                                                        aaGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGagagag                                                                           <  1:173435/148‑1 (MQ=255)
                                                                        aaGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGagagag                                                                           >  1:183382/1‑148 (MQ=255)
                                                                        aaGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAgg                                                                          <  4:492111/149‑1 (MQ=255)
                                                                         aGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAggg                                                                         <  4:680128/149‑1 (MQ=255)
                                                                         aGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAgg                                                                          <  2:183382/148‑1 (MQ=255)
                                                                             ccAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGgcgc                                                                      <  2:900628/148‑1 (MQ=255)
                                                                             ccAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGgcgc                                                                      <  2:590019/148‑1 (MQ=255)
                                                                             ccAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCt                                                                     >  3:422034/1‑149 (MQ=255)
                                                                               aaCCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTg                                                                    >  2:582944/1‑148 (MQ=255)
                                                                                 ccGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGt                                                                  >  1:636051/1‑148 (MQ=255)
                                                                                    cAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTtga                                                                                             >  2:393373/1‑118 (MQ=255)
                                                                                    cAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTtga                                                                                             <  1:393373/118‑1 (MQ=255)
                                                                                          aGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCaa                                                         <  1:534017/148‑1 (MQ=255)
                                                                                             gTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCgagag                                                                             >  1:280132/1‑125 (MQ=255)
                                                                                             gTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCg                                                      >  2:393516/1‑148 (MQ=255)
                                                                                              tGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGt                                                     >  2:115939/1‑148 (MQ=255)
                                                                                                  aCCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGTATGc                                                 >  1:433929/1‑148 (MQ=255)
                                                                                                   ccTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGTATGCt                                                <  2:214256/148‑1 (MQ=255)
                                                                                                         gCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGTATGCTGGAtttt                                         >  4:110387/1‑149 (MQ=255)
                                                                                                            gCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGGAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGTATGCTGGATTTTGCg                                      <  4:82058/149‑1 (MQ=255)
                                                                                                              tGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATTGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGagagag                                                                           >  2:751043/1‑110 (MQ=255)
                                                                                                              tGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATTGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGagagag                                                                           <  1:751043/110‑1 (MQ=255)
                                                                                                                  cgcATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTtg                                                                                              >  1:83353/1‑87 (MQ=255)
                                                                                                                  cgcATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTtg                                                                                              <  2:83353/87‑1 (MQ=255)
                                                                                                                    cATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGTATGCTGGATTTTGCGGCTacac                               >  2:270980/1‑148 (MQ=255)
                                                                                                                      ttGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGTATGCTGGATTTTGCGGCTACACTAc                            >  3:195341/1‑149 (MQ=255)
                                                                                                                       tGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGTATGCTGGATTTTGCGGCTACACTAc                            >  2:272926/1‑148 (MQ=255)
                                                                                                                              ggATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGTATGCTGGATTTTGCGGCTACACTACGTGGATaa                    <  3:110387/149‑1 (MQ=255)
                                                                                                                               gATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGTATGCTGGATTTTGCGGCTACACTACGTGGATAAc                   <  4:688266/149‑1 (MQ=255)
                                                                                                                                 tGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGTATGCTGGATTTTGCGGCTACACTACGTGGATAACg                  <  1:270980/148‑1 (MQ=255)
                                                                                                                                  ggCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGTATGCTGGATTTTGCGGCTACACTACGTGGATAACGa                 <  1:582944/148‑1 (MQ=255)
                                                                                                                                  ggCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGTATGCTGGATTTTGCGGCTACACTACGTGGATAACGa                 <  1:152595/148‑1 (MQ=255)
                                                                                                                                     cATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGt                                                     <  3:765526/109‑1 (MQ=255)
                                                                                                                                     cATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGt                                                     >  4:765526/1‑109 (MQ=255)
                                                                                                                                     cATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGTATGCTGGATTTTGCGGCTACACTACGTGGATAACGaataa             >  4:221259/1‑149 (MQ=255)
                                                                                                                                      aTGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGTATGCTGGATTTTGCGGCTACACTACGTGGATAACGaataat            >  3:761701/1‑149 (MQ=255)
                                                                                                                                      aTGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGTATGCTGGATTTTGCGGCTACACTACGTGGATAACGaataa             >  1:462781/1‑148 (MQ=255)
                                                                                                                                         aaTGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGTATGCTGGATTTTGCGGCTACACTACGTGGATAACGAAtaataa          <  1:896563/148‑1 (MQ=255)
                                                                                                                                         aaTGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGTATGCTGGATTTTGCGGCTACACTACGTGGATAACGAATAATAAg         >  4:580529/1‑149 (MQ=255)
                                                                                                                                                ttCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGTATGCTGGATTTTGCGGCTACACTACGTGGATAACGAATAATAAGGCGTAAc  >  4:503445/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGCTTATTGTGGCAACCGATCGGGGTATTTTCTACAAAATGCAGCAGGCGGTGCCAGATAAAGAGTTACTGGAAGCACCAACCGCAGGTGAGGGTGCAACCTGCCGCAGCTGCGCGCATTGTCCGTGGATGGCCATGAATGGCCTTCAGGCCATCGCAGAGGCATTAGAACAGGAAGGAAGCAATCACGAGGTTCATGTTGATGAAAGGCTGCGAGAGAGGGCGCTGGTGCCGCTCAATCGTATGCTGGATTTTGCGGCTACACTACGTGGATAACGAATAATAAGGCGTAAC  >  NC_000913/782854‑783146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: