Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 899,680 A→G 6.2% intergenic (+35/+164) rlmC → / ← artJ 23S rRNA m(5)U747 methyltransferase, SAM‑dependent/arginine ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913899,6800AG 6.2% 212.2 / 3.8 64intergenic (+35/+164)rlmC/artJ23S rRNA m(5)U747 methyltransferase, SAM‑dependent/arginine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (27/33);  new base G (2/2);  total (29/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.45e-01

GGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCC  >  NC_000913/899534‑899822
                                                                                                                                                  |                                                                                                                                              
ggCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACaaa                                                                                                                                                >  4:355555/1‑147 (MQ=255)
ggCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACaaa                                                                                                                                                <  3:355555/147‑1 (MQ=255)
  cGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTg                                                                                                                                             <  2:762738/148‑1 (MQ=255)
    aaaGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTgcgc                                                                                                                                          <  3:669712/149‑1 (MQ=255)
     aaGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTgcgc                                                                                                                                          >  2:29448/1‑148 (MQ=255)
       gATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCgt                                                                                                                                        <  2:482188/148‑1 (MQ=255)
        atatCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCgtt                                                                                                                                       >  2:626777/1‑148 (MQ=255)
         tatCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCgttgt                                                                                                                                     <  4:631191/149‑1 (MQ=255)
         tatCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCgttg                                                                                                                                      <  1:287696/148‑1 (MQ=255)
           tCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGttt                                                                                                                                   <  4:58443/149‑1 (MQ=255)
                    tGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGa                                                                                                                           <  1:140533/148‑1 (MQ=255)
                        tGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTACGCCGCATccg                                                                                                                       >  2:273960/1‑148 (MQ=255)
                              tCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTACGCCGCATCCGGCATaa                                                                                                                 <  1:273960/148‑1 (MQ=255)
                                  aTTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGcc                                                                                                            <  3:82603/149‑1 (MQ=255)
                                  aTTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGcc                                                                                                            >  3:208066/1‑149 (MQ=255)
                                           gTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                    <  1:651735/148‑1 (MQ=255)
                                               aGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                                                                >  2:32108/1‑148 (MQ=255)
                                               aGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                                               <  3:398910/149‑1 (MQ=255)
                                                  ttttCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAGGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaa                                                                                            >  4:315926/1‑149 (MQ=255)
                                                   tttCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa                                                                                           <  4:208066/149‑1 (MQ=255)
                                                       gATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCgtt                                                                                                                                       <  3:639136/101‑1 (MQ=255)
                                                       gATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCgtt                                                                                                                                       >  4:639136/1‑101 (MQ=255)
                                                       gATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCg                                                                                        <  2:490503/148‑1 (MQ=255)
                                                       gATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGt                                                                                       >  3:171608/1‑149 (MQ=255)
                                                         tatGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTg                                                                                      >  2:853979/1‑148 (MQ=255)
                                                            gTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCaaa                                                                                  <  3:610672/149‑1 (MQ=255)
                                                             ttCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAGGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAAt                                                                                 <  3:315926/149‑1 (MQ=255)
                                                              tCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                    >  1:768612/1‑129 (MQ=255)
                                                              tCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                    <  2:768612/129‑1 (MQ=255)
                                                                   cATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATa                                                                           <  4:781628/149‑1 (MQ=255)
                                                                      aCCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACa                                                                         <  1:29448/148‑1 (MQ=255)
                                                                      aCCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACa                                                                         <  1:32108/148‑1 (MQ=255)
                                                                             aCTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATg                                                                  <  1:853979/148‑1 (MQ=255)
                                                                               tATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAGGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGc                                                               >  4:189796/1‑149 (MQ=255)
                                                                               tATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAGGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGc                                                               >  3:59216/1‑149 (MQ=255)
                                                                               tATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATggg                                                                <  1:626777/148‑1 (MQ=255)
                                                                               tATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGc                                                               >  3:217669/1‑149 (MQ=255)
                                                                                 tGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCa                                                             >  4:517450/1‑149 (MQ=255)
                                                                                       gCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAgg                                                       <  4:171608/149‑1 (MQ=255)
                                                                                         tGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGcc                                                     <  3:517450/149‑1 (MQ=255)
                                                                                            cGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAAt                                                                                 <  2:426658/118‑1 (MQ=255)
                                                                                            cGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAAt                                                                                 >  1:426658/1‑118 (MQ=255)
                                                                                                  tgGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGc                                             >  2:493337/1‑148 (MQ=255)
                                                                                                     tGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTa                                          >  1:409964/1‑148 (MQ=255)
                                                                                                            aTAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCa                                  <  3:3528/149‑1 (MQ=255)
                                                                                                            aTAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCa                                  >  3:403705/1‑149 (MQ=255)
                                                                                                            aTAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCa                                  >  4:427032/1‑149 (MQ=255)
                                                                                                              aaaaaaGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCAGCCTACAAAAg                                                                                          >  1:708452/1‑91 (MQ=255)
                                                                                                              aaaaaaGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCAGCCTACAAAAg                                                                                          <  2:708452/91‑1 (MQ=255)
                                                                                                               aaaaaGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAgg                                >  2:231165/1‑148 (MQ=255)
                                                                                                               aaaaaGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCATAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAgg                                >  2:331576/1‑148 (MQ=255)
                                                                                                                tgaaGCCGCACATGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGc                               >  1:838546/3‑148 (MQ=255)
                                                                                                                 aaaGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCa                              >  1:237622/1‑148 (MQ=255)
                                                                                                                      cGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTa                        <  4:403705/149‑1 (MQ=255)
                                                                                                                       gCATGTGCGGCTTCAGATTGCTGACAAGGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTAc                       <  4:59216/149‑1 (MQ=255)
                                                                                                                       gCATGTGCGGCTTCAGATTGCTGACAAGGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTAc                       <  3:189796/149‑1 (MQ=255)
                                                                                                                       gCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGa                                                  >  3:623595/1‑122 (MQ=255)
                                                                                                                       gCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGa                                                  <  4:623595/122‑1 (MQ=255)
                                                                                                                       gCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTAc                       >  3:420693/1‑149 (MQ=255)
                                                                                                                       gCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTAc                       >  3:210336/1‑149 (MQ=255)
                                                                                                                       gCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTAc                       <  4:747753/149‑1 (MQ=255)
                                                                                                                                    cAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAg           <  1:493337/148‑1 (MQ=255)
                                                                                                                                         tGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCa      <  2:256012/148‑1 (MQ=255)
                                                                                                                                             gACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGcc  >  2:449580/1‑148 (MQ=255)
                                                                                                                                             gACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGc   <  1:786752/147‑1 (MQ=255)
                                                                                                                                             gACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGc   >  2:786752/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
GGCGAAAGATATCCGCGAACTGCCTGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCC  >  NC_000913/899534‑899822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: