Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,041,940 C→A 6.6% intergenic (+25/+90) appA → / ← etk phosphoanhydride phosphorylase/tyrosine‑protein kinase, role in O‑antigen capsule formation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,041,9400CA 6.6% 318.0 / 8.9 91intergenic (+25/+90)appA/etkphosphoanhydride phosphorylase/tyrosine‑protein kinase, role in O‑antigen capsule formation
Reads supporting (aligned to +/- strand):  ref base C (41/44);  new base A (4/2);  total (45/46)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CCGCCCGGAGAGGTGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGT  >  NC_000913/1041793‑1042086
                                                                                                                                                   |                                                                                                                                                  
ccgccCGGAGAGGTGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTAc                                                                                                                                                    >  2:844048/1‑148 (MQ=255)
     cGGAGAGGTGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAAt                                                                                                                                              <  4:238167/149‑1 (MQ=255)
     cGGAGAGGTGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGGTAACTGaa                                                                                                                                               <  2:321887/148‑1 (MQ=255)
     cGGAGAGGTGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTGTTAAGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAAAGCATTCAGGTAACTGaa                                                                                                                                               <  2:506536/148‑1 (MQ=255)
       gagaGGTGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAAt                                                                                                                                              >  4:56455/1‑147 (MQ=255)
       gagaGGTGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAAt                                                                                                                                              <  3:56455/147‑1 (MQ=255)
             tGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAg                                                                                                                                      <  3:204661/149‑1 (MQ=255)
             tGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAg                                                                                                                                      >  4:389653/1‑149 (MQ=255)
              gAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAg                                                                                                                                      >  2:534241/1‑148 (MQ=255)
                aaCTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCt                                                                                                                                   <  3:204113/149‑1 (MQ=255)
                   tGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCtgat                                                                                                                                <  3:587261/149‑1 (MQ=255)
                         tGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACaa                                                                                                                           >  1:210533/1‑148 (MQ=255)
                         tGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACaa                                                                                                                           <  2:531106/148‑1 (MQ=255)
                          ggCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAAc                                                                                                                         >  3:390265/1‑149 (MQ=255)
                           gCAGGATGTGAAGAGCGAAATGCGCAGCGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAAc                                                                                                                         >  2:265319/1‑148 (MQ=255)
                             aGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACgaa                                                                                                                      <  4:390265/149‑1 (MQ=255)
                             aGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACgaa                                                                                                                      <  3:688986/149‑1 (MQ=255)
                              ggATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACaa                                                                                                                           <  1:458568/143‑1 (MQ=255)
                              ggATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACaa                                                                                                                           >  2:458568/1‑143 (MQ=255)
                               gATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTAc                                                                                                                                                    >  4:27795/1‑117 (MQ=255)
                               gATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTAc                                                                                                                                                    <  3:27795/117‑1 (MQ=255)
                                aTGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCtga                                                                                                                                 >  2:882620/1‑135 (MQ=255)
                                aTGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCtga                                                                                                                                 <  1:882620/135‑1 (MQ=255)
                                    gAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTg                                                                                                                <  1:827151/148‑1 (MQ=255)
                                    gAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGt                                                                                                               <  4:674986/149‑1 (MQ=255)
                                     aaGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGt                                                                                                               <  2:52223/148‑1 (MQ=255)
                                          cGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAAt                                                                                                          <  1:265319/148‑1 (MQ=255)
                                           gAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATg                                                                                                         <  2:210533/148‑1 (MQ=255)
                                             aaTGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCg                                                                                                       >  2:605546/1‑148 (MQ=255)
                                             aaTGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGt                                                                                                      >  3:546353/1‑149 (MQ=255)
                                                  gcAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGAcc                                                                                                 >  4:16753/1‑149 (MQ=255)
                                                   cAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGAcc                                                                                                 >  2:454307/1‑148 (MQ=255)
                                                     gggCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCgg                                                                                               >  2:191415/1‑148 (MQ=255)
                                                        cATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGaaaa                                                                                           <  4:367683/149‑1 (MQ=255)
                                                         aTGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGaaaa                                                                                           <  1:371/148‑1 (MQ=255)
                                                         aTGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAg                                                                                          <  3:332090/149‑1 (MQ=255)
                                                                 gTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACgaag                                                                                                                     >  4:466529/1‑114 (MQ=255)
                                                                 gTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACgaag                                                                                                                     <  3:466529/114‑1 (MQ=255)
                                                                      cAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCAcgccg                                                                              >  1:291908/1‑148 (MQ=255)
                                                                          ttttACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATc                                                                          >  1:198284/1‑148 (MQ=255)
                                                                          ttttACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATc                                                                          >  1:728502/1‑148 (MQ=255)
                                                                             tACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGc                                                                      <  3:252086/149‑1 (MQ=255)
                                                                                gCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCAc                                                                   >  4:137837/1‑149 (MQ=255)
                                                                                  aaaTCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACtt                                                                 >  4:685676/1‑149 (MQ=255)
                                                                                    aTCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACttt                                                                >  1:249152/1‑148 (MQ=255)
                                                                                     tCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTc                                                               <  1:534241/148‑1 (MQ=255)
                                                                                       gTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGt                                                            >  3:829327/1‑149 (MQ=255)
                                                                                        tgaatgaaGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGtt                                                           >  4:875394/1‑149 (MQ=255)
                                                                                          aatgaaGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAgg                                                                                         <  2:203189/117‑1 (MQ=255)
                                                                                          aatgaaGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCGCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAgg                                                                                         >  1:203189/1‑117 (MQ=255)
                                                                                             gaaGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTAct                                                      <  3:303959/149‑1 (MQ=255)
                                                                                             gaaGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTAc                                                       <  1:454307/148‑1 (MQ=255)
                                                                                                gCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGt                                                            <  4:50107/140‑1 (MQ=255)
                                                                                                gCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGt                                                            >  3:50107/1‑140 (MQ=255)
                                                                                                gCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCtt                                                   >  4:647983/1‑149 (MQ=255)
                                                                                                 cACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCtt                                                   >  2:615900/1‑148 (MQ=255)
                                                                                                 cACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCtt                                                   <  2:249152/148‑1 (MQ=255)
                                                                                                    gCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACTTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTtctc                                               >  4:664232/1‑149 (MQ=255)
                                                                                                     cATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTtctc                                               >  1:697119/1‑148 (MQ=255)
                                                                                                       tACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCgg                                             <  2:35684/148‑1 (MQ=255)
                                                                                                       tACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCgg                                             <  1:191415/148‑1 (MQ=255)
                                                                                                        aCCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGa                                            <  2:728502/148‑1 (MQ=255)
                                                                                                        aCCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAg                                           >  4:198826/1‑149 (MQ=255)
                                                                                                           ggCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTa                                         <  1:800199/148‑1 (MQ=255)
                                                                                                                       gTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTaa                            <  3:664232/149‑1 (MQ=255)
                                                                                                                         aaTGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATa                          >  4:19817/1‑149 (MQ=255)
                                                                                                                          aTGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAg                         >  4:452587/1‑149 (MQ=255)
                                                                                                                           tGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAg                         >  1:48894/1‑148 (MQ=255)
                                                                                                                            gCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGtt                       >  3:845829/1‑149 (MQ=255)
                                                                                                                            gCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGtt                       >  3:363005/1‑149 (MQ=255)
                                                                                                                            gCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGt                        <  1:615900/148‑1 (MQ=255)
                                                                                                                                   tcagagCATTCAGGTAACTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCg                >  4:783223/3‑149 (MQ=255)
                                                                                                                                    aaGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGt               <  3:137837/149‑1 (MQ=255)
                                                                                                                                    aaGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCAAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCg                >  2:782680/1‑148 (MQ=255)
                                                                                                                                      gagCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTa              <  1:605546/148‑1 (MQ=255)
                                                                                                                                      gagCATTCAGGTAACTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTaa             >  3:155490/1‑149 (MQ=255)
                                                                                                                                       agCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGt                                          <  2:224188/119‑1 (MQ=255)
                                                                                                                                       agCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGt                                          >  1:224188/1‑119 (MQ=255)
                                                                                                                                       agCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTaa             >  1:744548/1‑148 (MQ=255)
                                                                                                                                       agCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAAc            >  4:529172/1‑149 (MQ=255)
                                                                                                                                       agCATTCAGGTAACTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTaa             >  1:481454/1‑148 (MQ=255)
                                                                                                                                       agCATTCAGGTAACTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTaa             >  2:593910/1‑148 (MQ=255)
                                                                                                                                          aTTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGa                                            <  4:267518/114‑1 (MQ=255)
                                                                                                                                          aTTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGa                                            >  3:267518/1‑114 (MQ=255)
                                                                                                                                           ttCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCttt                                                  <  4:720996/107‑1 (MQ=255)
                                                                                                                                           ttCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCttt                                                  >  3:720996/1‑107 (MQ=255)
                                                                                                                                            tCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTaa       <  3:685676/149‑1 (MQ=255)
                                                                                                                                            tCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTa        <  2:198284/148‑1 (MQ=255)
                                                                                                                                               gTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGc     <  2:291908/148‑1 (MQ=255)
                                                                                                                                               gTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCg    <  3:19817/149‑1 (MQ=255)
                                                                                                                                               gTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCg    <  4:845829/149‑1 (MQ=255)
                                                                                                                                                ttACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATaa                                  <  3:467739/118‑1 (MQ=255)
                                                                                                                                                ttACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATaa                                  >  4:467739/1‑118 (MQ=255)
                                                                                                                                                 tACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGt  <  4:829327/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCGCCCGGAGAGGTGAAACTGACCCTGGCAGGATGTGAAGAGCGAAATGCGCAGGGCATGTGTTCGTTGGCAGGTTTTACGCAAATCGTGAATGAAGCACGCATACCGGCGTGCAGTTTGTAATGCATAAAAAAGAGCATTCAGTTACCTGAATGCTCTGAGGCTGATGACAAACGAAGAACTGTCTAATGCGTAGACCGGAAAAGGCGTTCACGCCGCATCCGGCCACTTTCAGTTTTACTCTTTCTCGGAGTAACTATAACCGTAATAGTTATAGCCGTAACTGTAAGCGGT  >  NC_000913/1041793‑1042086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: