Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,159,335 G→T 8.8% intergenic (+33/+27) ptsG → / ← fhuE fused glucose‑specific PTS enzymes: IIB component/IIC component/ferric‑rhodotorulic acid outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,159,3350GT 8.8% 315.2 / 16.1 91intergenic (+33/+27)ptsG/fhuEfused glucose‑specific PTS enzymes: IIB component/IIC component/ferric‑rhodotorulic acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (35/48);  new base T (3/5);  total (38/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

GAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAG  >  NC_000913/1159188‑1159482
                                                                                                                                                   |                                                                                                                                                   
gaagaaACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAg                                                                                                                                                     <  2:444807/148‑1 (MQ=255)
gaagaaACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGa                                                                                                                                                    <  3:257505/149‑1 (MQ=255)
 aagaaACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGa                                                                                                                                                    <  1:552340/148‑1 (MQ=255)
 aagaaACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGAt                                                                                                                                                   <  4:40054/149‑1 (MQ=255)
   gaaACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATg                                                                                                                                                  >  2:433453/1‑148 (MQ=255)
    aaaCTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTTGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATgg                                                                                                                                                 >  2:27964/1‑148 (MQ=255)
    aaaCTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATgg                                                                                                                                                 >  2:688061/1‑148 (MQ=255)
     aaCTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCATCTGGCt                                                                                                                                               <  3:770466/149‑1 (MQ=255)
     aaCTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCt                                                                                                                                               <  4:282054/149‑1 (MQ=255)
      aCTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCt                                                                                                                                               <  1:306577/148‑1 (MQ=255)
       cTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGc                                                                                                                                             <  4:624958/149‑1 (MQ=255)
           gcgcAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGAACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCATCTGGCTGCCtt                                                                                                                                          <  2:731198/148‑1 (MQ=255)
            cgcAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCATCTGGCTGc                                                                                                                                             <  2:870540/144‑1 (MQ=255)
            cgcAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCATCTGGCTGc                                                                                                                                             >  1:870540/1‑144 (MQ=255)
               aGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCtttttt                                                                                                                                      <  2:227094/148‑1 (MQ=255)
               aGCGGGCGTAGTGGTTGCTGGTTCTGATGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCTAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCTGCCAGATGGCTGCCtttttt                                                                                                                                      <  1:655739/148‑1 (MQ=255)
                  gggCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAg                                                                                                                                  <  3:487247/149‑1 (MQ=255)
                    gCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAgg                                                                                                                                 <  1:17536/148‑1 (MQ=255)
                           ggTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGgt                                                                                                                                <  3:622124/142‑1 (MQ=255)
                           ggTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGgt                                                                                                                                >  4:622124/1‑142 (MQ=255)
                                    ttCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGAt                                                                                                                <  3:691682/149‑1 (MQ=255)
                                          tgtTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTg                                                                                                           <  1:700536/148‑1 (MQ=255)
                                                      tttCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTg                                                                                               >  2:823514/1‑148 (MQ=255)
                                                          ggTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAAtt                                                                                          >  4:180762/1‑149 (MQ=255)
                                                            tACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCATCTGGCTGcc                                                                                                                                            <  4:248599/97‑1 (MQ=255)
                                                            tACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCATCTGGCTGcc                                                                                                                                            >  3:248599/1‑97 (MQ=255)
                                                            tACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTa                                                                                         >  1:162471/1‑148 (MQ=255)
                                                                 aaTCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGgt                                                                                   <  3:365621/149‑1 (MQ=255)
                                                                  aTCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATa                                                                                                               >  1:831859/1‑120 (MQ=255)
                                                                  aTCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATa                                                                                                               <  2:831859/120‑1 (MQ=255)
                                                                     cGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGc                                                                               <  4:176814/149‑1 (MQ=255)
                                                                           ccTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGa                                                                         >  4:122320/1‑149 (MQ=255)
                                                                            cTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGaaa                                                                                            >  4:166228/1‑129 (MQ=255)
                                                                            cTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGaaa                                                                                            <  3:166228/129‑1 (MQ=255)
                                                                            cTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGAc                                                                        >  4:370347/1‑149 (MQ=255)
                                                                             tGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGAc                                                                        >  1:47578/1‑148 (MQ=255)
                                                                             tGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGAc                                                                        >  2:619123/1‑148 (MQ=255)
                                                                              gAAAACCGATATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTTATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGa                                                                      <  3:370347/149‑1 (MQ=255)
                                                                              gAAAACCGAGATGGTTGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGa                                                                                              >  2:887532/1‑125 (MQ=255)
                                                                              gAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGa                                                                                              <  1:887532/125‑1 (MQ=255)
                                                                              gAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGc                                                                               >  2:284414/1‑140 (MQ=255)
                                                                              gAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGc                                                                               <  1:284414/140‑1 (MQ=255)
                                                                              gAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACg                                                                       >  1:670896/1‑148 (MQ=255)
                                                                              gAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGa                                                                      <  4:897949/149‑1 (MQ=255)
                                                                                aaaCCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGAt                                                                     <  1:904497/148‑1 (MQ=255)
                                                                                aaaCCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGAt                                                                     <  1:27964/148‑1 (MQ=255)
                                                                                aaaCCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATa                                                                    <  3:122320/149‑1 (MQ=255)
                                                                                  aCCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGAt                                                                                                                <  2:116703/103‑1 (MQ=255)
                                                                                  aCCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGAt                                                                                                                >  1:116703/1‑103 (MQ=255)
                                                                                  aCCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAg                                                                   >  2:453045/1‑148 (MQ=255)
                                                                                  aCCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAg                                                                   >  2:323349/1‑148 (MQ=255)
                                                                                  aCCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAg                                                                   >  2:291690/1‑148 (MQ=255)
                                                                                     gagaTGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCTGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAcc                                                               >  4:268596/1‑149 (MQ=255)
                                                                                        atggatgAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCtt                                                             <  1:433453/148‑1 (MQ=255)
                                                                                         tggatgAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTc                                                            <  1:823514/148‑1 (MQ=255)
                                                                                           gatgAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCAc                                                         >  3:229484/1‑149 (MQ=255)
                                                                                             tgAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGt                                                       >  3:43031/1‑149 (MQ=255)
                                                                                               aGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTGTTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTg                                                     <  4:825120/149‑1 (MQ=255)
                                                                                                gTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTgg                                                    >  4:188587/1‑149 (MQ=255)
                                                                                                  aCATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAAt                                                                                                                    <  2:246733/83‑1 (MQ=255)
                                                                                                  aCATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAAt                                                                                                                    >  1:246733/1‑83 (MQ=255)
                                                                                                    aTCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTAt                                                 >  1:56836/1‑148 (MQ=255)
                                                                                                       cGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGt                                              <  1:323349/148‑1 (MQ=255)
                                                                                                       cGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGt                                              <  1:453045/148‑1 (MQ=255)
                                                                                                       cGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGt                                              <  1:619123/148‑1 (MQ=255)
                                                                                                            ccACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTc                                                            >  2:522008/1‑129 (MQ=255)
                                                                                                            ccACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTc                                                            <  1:522008/129‑1 (MQ=255)
                                                                                                              aCTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGt                                              <  1:321982/141‑1 (MQ=255)
                                                                                                              aCTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGt                                              >  2:321982/1‑141 (MQ=255)
                                                                                                              aCTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTg                                      >  4:704511/1‑149 (MQ=255)
                                                                                                                  aTCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGGTGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATAc                                                                                                              <  3:288282/73‑1 (MQ=255)
                                                                                                                  aTCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGGTGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATAc                                                                                                              >  4:288282/1‑73 (MQ=255)
                                                                                                                    ccGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGaa                                 <  2:189265/148‑1 (MQ=255)
                                                                                                                      gTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAg                              <  3:180762/149‑1 (MQ=255)
                                                                                                                        aaGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAgg                             >  2:601925/1‑148 (MQ=255)
                                                                                                                         aGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATg                                                                                                  >  4:818360/1‑78 (MQ=255)
                                                                                                                         aGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATg                                                                                                  <  3:818360/78‑1 (MQ=255)
                                                                                                                         aGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGt                            <  1:291690/148‑1 (MQ=255)
                                                                                                                           aCGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTa                          <  1:688061/148‑1 (MQ=255)
                                                                                                                                  ggAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGt                   <  2:670896/148‑1 (MQ=255)
                                                                                                                                   gagaCTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGtt                  <  2:47578/148‑1 (MQ=255)
                                                                                                                                    agaCTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGt                   >  3:421824/1‑146 (MQ=255)
                                                                                                                                    agaCTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGt                   <  4:421824/146‑1 (MQ=255)
                                                                                                                                      aCTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCTGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTcccc              <  3:268596/149‑1 (MQ=255)
                                                                                                                                        tAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTcc                >  2:448375/1‑145 (MQ=255)
                                                                                                                                        tAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTcc                <  1:448375/145‑1 (MQ=255)
                                                                                                                                        aaaGGCAGCCATCTGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCgg                                                                                    >  2:171680/2‑77 (MQ=255)
                                                                                                                                        aaaGGCAGCCATCTGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCgg                                                                                    <  1:171680/76‑1 (MQ=255)
                                                                                                                                           ggCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGAt                                                                     >  1:321277/1‑89 (MQ=255)
                                                                                                                                           ggCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGAt                                                                     <  2:321277/89‑1 (MQ=255)
                                                                                                                                                  aGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGaa   <  1:13700/148‑1 (MQ=255)
                                                                                                                                                  aGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAg  <  3:704511/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGACGTTCCCCTGTAACGAGAAG  >  NC_000913/1159188‑1159482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: