Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,498,894 C→G 12.5% intergenic (+19/‑44) ydcH → / → rimL DUF465 family protein/ribosomal‑protein‑L7/L12‑serine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,498,8940CG12.5% 202.7 / 18.8 64intergenic (+19/‑44)ydcH/rimLDUF465 family protein/ribosomal‑protein‑L7/L12‑serine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (30/26);  new base G (4/4);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

TTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATAAAACCTG  >  NC_000913/1498748‑1499038
                                                                                                                                                  |                                                                                                                                                
ttGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCgccagcc                                                                                                                                                 >  1:753188/1‑148 (MQ=255)
 tGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAACGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTATCCTAAGCCgccagcca                                                                                                                                                <  1:264221/148‑1 (MQ=255)
 tGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGccagccag                                                                                                                                               <  3:294969/149‑1 (MQ=255)
   ccAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAggc                                                                                                                                             <  4:49462/149‑1 (MQ=255)
     aGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAggcg                                                                                                                                            >  2:867398/1‑148 (MQ=255)
       aaaGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAggcggc                                                                                                                                          >  2:504240/1‑148 (MQ=255)
         aggaaggaTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCttt                                                                                                                                       <  4:239839/149‑1 (MQ=255)
         aggaaggTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCTGGCTGGCGGCtta                                                                                                                                       <  3:159813/149‑2 (MQ=255)
          ggaaggTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCttt                                                                                                                                       >  1:228875/1‑148 (MQ=255)
           gaaggTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCtttt                                                                                                                                      >  2:550186/1‑148 (MQ=255)
           gaaggTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCtttt                                                                                                                                      <  1:332443/148‑1 (MQ=255)
            aaggTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCTGGCTGGCGGCtta                                                                                                                                       >  3:111011/1‑145 (MQ=255)
            aaggTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCtttttt                                                                                                                                    <  3:88506/149‑1 (MQ=255)
            aaggTTCCAACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTAAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGTTGTAAACTTTCCTAAGCCGCCTGGCTGGCGGCtta                                                                                                                                       <  4:111011/146‑2 (MQ=255)
             aggTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTa                                                                                                                                   >  4:35149/1‑149 (MQ=255)
                    gACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTg                                                                                                                            >  3:74134/1‑149 (MQ=255)
                               gTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGa                                                                                                                  <  1:867398/148‑1 (MQ=255)
                               gTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGAc                                                                                                                 <  3:156494/149‑1 (MQ=255)
                                   aaTGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCTGGCTGGCGGCttag                                                                                                                                      >  3:687600/1‑122 (MQ=255)
                                   aaTGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCTGGCTGGCGGCttag                                                                                                                                      <  4:687600/124‑3 (MQ=255)
                                                tCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACt                                                                                                 >  2:83429/1‑148 (MQ=255)
                                                 ccGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTg                                                                                                >  1:114011/1‑148 (MQ=255)
                                                    cATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAAc                                                                                            <  4:102739/149‑1 (MQ=255)
                                                        aaaaaaCAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATa                                                                                        >  3:664396/1‑149 (MQ=255)
                                                         aaaaaCAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATaa                                                                                       <  3:584668/149‑1 (MQ=255)
                                                         aaaaaCAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATaa                                                                                       >  4:584668/1‑149 (MQ=255)
                                                          aaaaCAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATaaa                                                                                      >  4:89183/1‑149 (MQ=255)
                                                             aCAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGt                                                                                   >  3:781295/1‑149 (MQ=255)
                                                                aaaGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAg                                                                                                       <  1:295482/126‑1 (MQ=255)
                                                                aaaGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAg                                                                                                       >  2:295482/1‑126 (MQ=255)
                                                                     tACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGaa                                                                            <  2:228875/148‑1 (MQ=255)
                                                                             aaaGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGaa                                                                   >  4:340741/1‑149 (MQ=255)
                                                                                gatgagatgCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTa                                                                >  3:619377/1‑149 (MQ=255)
                                                                                 atgagatgCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAg                                                                                >  2:821876/1‑132 (MQ=255)
                                                                                 atgagatgCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAg                                                                                <  1:821876/132‑1 (MQ=255)
                                                                                  tgagatgCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACa                                                              <  4:619377/149‑1 (MQ=255)
                                                                                      atgCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGc                                                           >  1:636974/1‑148 (MQ=255)
                                                                                       tgCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTg                                                         <  3:395101/149‑1 (MQ=255)
                                                                                         cTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGt                                                        <  2:373282/148‑1 (MQ=255)
                                                                                              aaTCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGa                                                  >  4:212802/1‑149 (MQ=255)
                                                                                                 ccTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGaaaa                                               <  4:781295/149‑1 (MQ=255)
                                                                                                 ccTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGaaa                                                <  2:636974/148‑1 (MQ=255)
                                                                                                   tGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGaaa                                                <  3:571973/146‑1 (MQ=255)
                                                                                                   tGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGaaa                                                >  4:571973/1‑146 (MQ=255)
                                                                                                     cagcagGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCAc                                           <  3:340741/149‑1 (MQ=255)
                                                                                                        cagGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGt                                         >  2:226455/1‑148 (MQ=255)
                                                                                                         agGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCa                                       >  4:466244/1‑149 (MQ=255)
                                                                                                            agagCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAAc                                    <  3:305996/149‑1 (MQ=255)
                                                                                                              agCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAAcc                                   <  1:504240/148‑1 (MQ=255)
                                                                                                                cGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACctc                                 >  1:787326/1‑148 (MQ=255)
                                                                                                                 gTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCtt                               >  3:501286/1‑149 (MQ=255)
                                                                                                                         ggTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGtt                       <  3:89183/149‑1 (MQ=255)
                                                                                                                         ggTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGt                        <  1:550186/148‑1 (MQ=255)
                                                                                                                                aCTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAAt                                                                  <  1:303189/99‑1 (MQ=255)
                                                                                                                                aCTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAAt                                                                  >  2:303189/1‑99 (MQ=255)
                                                                                                                                      cTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATc                           <  1:116548/132‑1 (MQ=255)
                                                                                                                                      cTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATc                           >  2:116548/1‑132 (MQ=255)
                                                                                                                                       tAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATa         >  3:844962/1‑149 (MQ=255)
                                                                                                                                       aaaGCCGCCTGGCTGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCAAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATa         >  4:362985/2‑149 (MQ=255)
                                                                                                                                        aaGCCGCCTGGCTGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATaa        >  4:274606/1‑149 (MQ=255)
                                                                                                                                        aaGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAAt                                              <  3:13098/111‑1 (MQ=255)
                                                                                                                                        aaGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAAt                                              >  4:13098/1‑111 (MQ=255)
                                                                                                                                         aGCCGCCTGGCTGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATaaa       <  3:274606/149‑1 (MQ=255)
                                                                                                                                         aGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATaaa       >  3:246774/1‑149 (MQ=255)
                                                                                                                                           ccgccAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATaaaa      >  2:59288/1‑148 (MQ=255)
                                                                                                                                            cgccAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACa                                                              <  4:334857/91‑1 (MQ=255)
                                                                                                                                            cgccAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACa                                                              >  3:334857/1‑91 (MQ=255)
                                                                                                                                              ccagccagGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTCAAACGATAAAAGTAAGCGAATCACTTGAATTAAATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATAAAACCTg  <  4:664396/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TTGCCAGAAAGGAAGGTTCCGACGGTCGAGGGTACAATGCGGAAGTGGTCCGCATGAAAAAACAAAAGCTACAGTTAAAAGATGAGATGCTCAAAATCCTGCAGCAGGAGAGCGTCAAAGAGGTGTAAACTTTCCTAAGCCGCCAGCCAGGCGGCTTTTTTAACAACTGCATGGATTGACTGGAGATAAGATGACTGAAACGATAAAAGTAAGCGAATCACTTGAATTACATGCTGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATAAAACCTG  >  NC_000913/1498748‑1499038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: