Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,552,377 G→A 7.3% intergenic (+386/+21) fdnI → / ← yddM formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,552,3770GA 7.3% 258.9 / 9.1 82intergenic (+386/+21)fdnI/yddMformate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (42/34);  new base A (4/2);  total (46/36)
Fisher's exact test for biased strand distribution p-value = 6.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

CAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTGCCGATCACCACGGAAAGTTTAATCG  >  NC_000913/1552230‑1552525
                                                                                                                                                   |                                                                                                                                                    
cAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGgcg                                                                                                                                                      >  2:584981/1‑148 (MQ=34)
  aCGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCtt                                                                                                                                                   <  3:860516/149‑1 (MQ=35)
        gCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTtatac                                                                                                                                              <  1:427945/148‑2 (MQ=38)
        gCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATaaa                                                                                                                                             <  3:131057/149‑1 (MQ=255)
            tGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTg                                                                                                                                          <  2:409611/148‑1 (MQ=255)
                          aaggcgTTCACGCCGCATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTg                                                                                                                            >  1:828411/3‑148 (MQ=255)
                               gttcaCGCCGCATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTaa                                                                                                                       <  2:272156/144‑1 (MQ=255)
                               gTGAACGCCTTATCCGGCGTGAAAACCGTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCACTTTATAAAGCGCCAttt                                                                                                                                    <  4:739803/135‑1 (MQ=14)
                               gTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGACACTTTATAAAGCGCCAttt                                                                                                                                    >  3:739803/1‑135 (MQ=255)
                                 gAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCa                                                                                                                    >  4:145108/1‑149 (MQ=255)
                                  aaCGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAg                                                                                                                   >  4:594511/1‑149 (MQ=255)
                                   acgccgcaTCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAg                                                                                                                   <  2:828411/141‑1 (MQ=255)
                                       cTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGc                                                                                                              >  3:293321/1‑149 (MQ=255)
                                       cTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGc                                                                                                              >  3:91978/1‑149 (MQ=255)
                                       cTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGc                                                                                                              <  3:111966/149‑1 (MQ=255)
                                       cTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGc                                                                                                              >  4:111966/1‑149 (MQ=255)
                                        ttATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGc                                                                                                              >  1:614017/1‑148 (MQ=255)
                                            ccGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAAt                                                                                                         >  4:188886/1‑149 (MQ=255)
                                                 aTGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGa                                                                                                    <  4:226580/149‑1 (MQ=255)
                                                     aaaCCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTCTTTTATTGCGTAACCAGACGGCGTAATCGCGAcaca                                                                                                >  4:241377/1‑149 (MQ=255)
                                                       aCCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACAt                                                                                               >  2:771981/1‑148 (MQ=255)
                                                          cTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCAc                                                                                           >  4:327406/1‑149 (MQ=255)
                                                           ttCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCAc                                                                                           >  2:624861/1‑148 (MQ=255)
                                                           ttCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACt                                                                                          >  3:346309/1‑149 (MQ=255)
                                                                aTCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGtttt                                                                                     <  4:469709/149‑1 (MQ=255)
                                                                 tCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGttttt                                                                                    <  3:432382/149‑1 (MQ=255)
                                                                 tCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGtttt                                                                                     >  1:354603/1‑148 (MQ=255)
                                                                   cAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGtttttt                                                                                   <  1:771981/148‑1 (MQ=255)
                                                                   cAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTtc                                                                                  <  3:145108/149‑1 (MQ=255)
                                                                    aaTAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTtc                                                                                  >  1:637061/1‑148 (MQ=255)
                                                                    aaTAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTtc                                                                                  <  2:614017/148‑1 (MQ=255)
                                                                      tAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTtctg                                                                                >  2:83822/1‑148 (MQ=255)
                                                                          ttGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTtctgcctc                                                                            <  1:584981/148‑1 (MQ=255)
                                                                          ttGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTtctgc                                                                               >  3:351256/1‑145 (MQ=255)
                                                                          ttGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTtctgc                                                                               <  4:351256/145‑1 (MQ=255)
                                                                                tGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGa                                                                     <  4:346309/149‑1 (MQ=255)
                                                                                 gAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGa                                                                     >  2:680763/1‑148 (MQ=255)
                                                                                  aaCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTTTGCCTCTGCCAGACt                                                                   >  4:634326/1‑149 (MQ=255)
                                                                                  aaCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACt                                                                   >  3:851425/1‑149 (MQ=255)
                                                                                   aCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACt                                                                   >  2:732320/1‑148 (MQ=255)
                                                                                    cGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTcc                                                                 <  3:188886/149‑1 (MQ=255)
                                                                                      tgtAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCa                                                                >  1:181452/1‑148 (MQ=255)
                                                                                      tgtAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAg                                                               <  3:760472/149‑1 (MQ=255)
                                                                                          ggCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCTAGGCg                                                            <  2:647740/148‑1 (MQ=255)
                                                                                               gATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTaa                                                                                                                       >  3:108812/1‑84 (MQ=255)
                                                                                               gATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTaa                                                                                                                       <  4:108812/84‑1 (MQ=255)
                                                                                                   aGCGTAGCGCATCAGGCAATGTTGTGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGa                                                   >  1:131539/1‑148 (MQ=255)
                                                                                                    gCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGAtt                                                 <  3:327406/149‑1 (MQ=255)
                                                                                                     cGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTCTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTc                                                <  3:241377/149‑1 (MQ=255)
                                                                                                       tAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCa                                                                >  2:183190/1‑131 (MQ=255)
                                                                                                       tAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCa                                                                <  1:183190/131‑1 (MQ=255)
                                                                                                          cgcATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGcc                                            >  1:317458/1‑148 (MQ=255)
                                                                                                              tCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGttt                                                         <  1:56414/131‑1 (MQ=255)
                                                                                                              tCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGttt                                                         >  2:56414/1‑131 (MQ=255)
                                                                                                                aGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTg                                     >  3:753467/1‑149 (MQ=255)
                                                                                                                 ggCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTgc                                    >  3:152947/1‑149 (MQ=255)
                                                                                                                  gCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTgc                                    >  2:414229/1‑148 (MQ=255)
                                                                                                                         tGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAAc                             >  2:501494/1‑148 (MQ=255)
                                                                                                                           cGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTg                           <  2:317458/148‑1 (MQ=255)
                                                                                                                           cGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTGc                          <  4:293321/149‑1 (MQ=255)
                                                                                                                             tttGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTGCCg                        >  3:636785/1‑149 (MQ=255)
                                                                                                                                gTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTGCCGATc                     >  3:114308/1‑149 (MQ=255)
                                                                                                                                 tcatcaGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTGCCGATca                    >  3:52085/1‑149 (MQ=255)
                                                                                                                                    tcaGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTGCCGATcacca                 >  4:572466/1‑149 (MQ=255)
                                                                                                                                       gTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTCCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCCCATTTGCGGCGAACTGCCGATCACCACg               >  1:62098/1‑148 (MQ=255)
                                                                                                                                        ttCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTGCCGATCACCACgg              >  2:563783/1‑148 (MQ=255)
                                                                                                                                         tCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCCCTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTGCCGATCACCACGGa             <  1:732320/148‑1 (MQ=255)
                                                                                                                                         tCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCa                                                                <  2:458945/97‑1 (MQ=255)
                                                                                                                                         tCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCa                                                                >  1:458945/1‑97 (MQ=255)
                                                                                                                                          cTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTGCCGATCACCACGGaa            <  2:131539/148‑1 (MQ=255)
                                                                                                                                          cTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTGCCGATCACCACGGaa            <  1:680763/148‑1 (MQ=255)
                                                                                                                                          cTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTGCCGATCACCACGGaa            <  1:414229/148‑1 (MQ=255)
                                                                                                                                           aaaaTGGCACTTTATAAAGCGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTGCCGATCACCACGGAAAg          >  3:371552/2‑149 (MQ=255)
                                                                                                                                            aaaTGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTGCCGATCACCACGGAAAg          <  1:342357/148‑1 (MQ=255)
                                                                                                                                            aaaTGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTGCCGATCACCACGGAAAg          >  1:118058/1‑148 (MQ=255)
                                                                                                                                            aaaTGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTGCCGATCACCACGGAAAg          >  1:470767/1‑148 (MQ=255)
                                                                                                                                            aaaTGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTGCCGATCACCACGGAAAGt         <  4:851425/149‑1 (MQ=255)
                                                                                                                                            aaaTGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCGGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTGCCGATCACCACGGAAAg          >  1:630986/1‑148 (MQ=255)
                                                                                                                                             aaTGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTg                                                       <  3:675981/102‑1 (MQ=255)
                                                                                                                                             aaTGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTg                                                       >  4:675981/1‑102 (MQ=255)
                                                                                                                                               tGGCACTTTATAAAGCGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTg                                     <  1:67020/118‑1 (MQ=255)
                                                                                                                                               tGGCACTTTATAAAGCGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTg                                     >  2:67020/1‑118 (MQ=255)
                                                                                                                                               tGGCACTTTATAAAGCGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTGCCGATCACCACGGAAAGttt       >  2:717815/1‑148 (MQ=255)
                                                                                                                                                  cgcTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTGCCGATCACCACGGAAAGTTTAAt    <  2:354603/148‑1 (MQ=255)
                                                                                                                                                   gcTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTGCCGATCACCACGGAAAGTTTAATCg  >  3:49306/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCGTAATCGCGACACATCCACTGTTTTTTCTGCCTCTGCCAGACTCCAGGCGTTTTGCAGATTCAGCCACATTTGCGGCGAACTGCCGATCACCACGGAAAGTTTAATCG  >  NC_000913/1552230‑1552525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: