Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,557,343 C→T 6.5% T232T (ACG→ACA) ‡ ddpF ← D,D‑dipeptide ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,557,3430CT 6.5% 293.1 / 8.1 92T232T (ACG→ACA) ‡ddpFD,D‑dipeptide ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (47/39);  new base T (2/4);  total (49/43)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.22e-01

GGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAA  >  NC_000913/1557197‑1557489
                                                                                                                                                  |                                                                                                                                                  
gggCAACGTTCTTAGAAAAAACAGCCTAGTGGCAACGTGAGGTTTCCCGGCAGATCCGTTTTACGAAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGAAATAATCGGGTGTATGGATGTGCAGGTGCCg                                                                                                                                                   <  2:442116/148‑1 (MQ=255)
 ggCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTc                                                                                                                                                 <  4:502948/149‑1 (MQ=255)
    aaCGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGc                                                                                                                                              >  3:220340/1‑149 (MQ=255)
       gTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCAcc                                                                                                                                           >  4:752709/1‑149 (MQ=255)
        ttCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCAcc                                                                                                                                           >  1:552143/1‑148 (MQ=255)
         tCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACct                                                                                                                                          <  1:769125/148‑1 (MQ=255)
          cGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACctgc                                                                                                                                        >  4:344638/1‑149 (MQ=255)
           gTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgca                                                                                                                                       <  3:288146/149‑2 (MQ=255)
            tAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctgcac                                                                                                                                      <  4:631660/149‑3 (MQ=255)
            tAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACctgctg                                                                                                                                      >  4:358830/1‑149 (MQ=255)
             aGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACctgctg                                                                                                                                      <  1:451433/148‑1 (MQ=255)
              gAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctg                                                                                                                                         >  2:613620/1‑144 (MQ=255)
              gAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCTGACGGCACctg                                                                                                                                         <  1:613620/144‑1 (MQ=255)
               aaaaaaCAGCCTTGCGGCAGCGTGCGGTATCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGTGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGcgcg                                                                                                                                  >  3:749970/1‑149 (MQ=255)
                aaaaaCAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGAGCAGGTGCCGTCAGCACCTGCAGtgcg                                                                                                                                  >  4:781566/1‑149 (MQ=255)
                  aaaCAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTc                                                                                                                                <  3:358830/149‑1 (MQ=255)
                           tGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGtt                                                                                                                        >  1:558921/1‑148 (MQ=255)
                            ccGGCAACGTGCGGTTTCCCGGCAGATCCGGTTTACGAAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCt                                                                                                                      <  3:674073/148‑1 (MQ=255)
                                  aCGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAAt                                                                                                                 >  2:215277/1‑148 (MQ=255)
                                     tGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGt                                                                                                                         <  1:198369/137‑1 (MQ=255)
                                     tGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGt                                                                                                                         >  2:198369/1‑137 (MQ=255)
                                     tGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTg                                                                                                              >  1:257730/1‑148 (MQ=255)
                                         gTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCga                                                                                                         <  3:672975/149‑1 (MQ=255)
                                          tttCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAg                                                                                                                                               <  2:344417/110‑1 (MQ=255)
                                          tttCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAg                                                                                                                                               >  1:344417/1‑110 (MQ=255)
                                                   aGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCAc                                                                                               <  3:752709/149‑1 (MQ=255)
                                                    gATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCAcc                                                                                              <  4:626225/149‑1 (MQ=255)
                                                     aTCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCg                                                                                             >  3:392079/1‑149 (MQ=255)
                                                     aTCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGGTCTACAATCTGGCCGAGATACATCACCg                                                                                             >  3:693520/1‑149 (MQ=255)
                                                      tCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGc                                                                                            >  3:485863/1‑149 (MQ=255)
                                                        cGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCa                                                                                          <  3:1463/149‑1 (MQ=255)
                                                          ttttACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAcc                                                                                        >  4:134200/1‑149 (MQ=255)
                                                           tttACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAcc                                                                                        <  2:552143/148‑1 (MQ=255)
                                                           tttACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAcc                                                                                        <  2:158265/148‑1 (MQ=255)
                                                              aCGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGa                                                                                     <  1:666284/148‑1 (MQ=255)
                                                               cGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAc                                                                                         >  4:241281/1‑143 (MQ=255)
                                                               cGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAc                                                                                         <  3:241281/143‑1 (MQ=255)
                                                                 tAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCg                                                                                  >  1:861837/1‑148 (MQ=255)
                                                                 tAATGCCCATTCTTCCTCCAGCGGGTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGGTCTACAATCTGTCCGAGATACATCACCGCCGCCCGATCGc                                                                                 >  3:764784/1‑149 (MQ=255)
                                                                    tGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTc                                                                               <  1:215277/148‑1 (MQ=255)
                                                                       ccATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTTCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCata                                                                            >  2:336257/1‑148 (MQ=255)
                                                                       ccATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCatat                                                                           >  4:183562/1‑149 (MQ=255)
                                                                        cATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCatat                                                                           >  1:300510/1‑148 (MQ=255)
                                                                         aTTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGa                                                                         >  3:555882/1‑149 (MQ=255)
                                                                          ttcttcCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGa                                                                         <  2:257730/148‑1 (MQ=255)
                                                                          ttcttcCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGAc                                                                        <  4:485863/149‑1 (MQ=255)
                                                                           tcttcCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGAc                                                                        >  1:727568/1‑148 (MQ=255)
                                                                              tcctccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTAt                                                                    >  4:653477/1‑149 (MQ=255)
                                                                                  ccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCAcc                                                                >  4:219816/1‑149 (MQ=255)
                                                                                  ccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCAc                                                                 >  2:518709/1‑148 (MQ=255)
                                                                                  ccAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCAc                                                                 >  1:558028/1‑148 (MQ=255)
                                                                                    aGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCg                                                               <  2:727568/148‑1 (MQ=255)
                                                                                      cGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGcc                                                                                                                             >  2:766451/1‑84 (MQ=255)
                                                                                      cGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGcc                                                                                                                             <  1:766451/84‑1 (MQ=255)
                                                                                        gTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAc                                                                                         <  3:1434/118‑1 (MQ=255)
                                                                                        gTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCAc                                                                                         >  4:1434/1‑118 (MQ=255)
                                                                                         tttATCAATGGCGGGGAAGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACAt                                                         >  3:70172/1‑149 (MQ=255)
                                                                                          ttATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACAt                                                         >  1:325263/1‑148 (MQ=255)
                                                                                            aTCAATGGCGGGGAGGGAATCCAGCTATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtg                                                       <  2:537313/148‑1 (MQ=255)
                                                                                            aTCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtg                                                       <  1:809764/148‑1 (MQ=255)
                                                                                             tCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgt                                                      >  2:680001/1‑148 (MQ=255)
                                                                                              cAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgtgt                                                    <  3:344638/149‑1 (MQ=255)
                                                                                              cAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgtg                                                     <  2:861837/148‑1 (MQ=255)
                                                                                              cAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgtg                                                     <  1:680001/148‑1 (MQ=255)
                                                                                                aTGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATtgtgtg                                                   >  1:341394/1‑148 (MQ=255)
                                                                                                     ggggAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATc                                              <  2:679588/148‑1 (MQ=255)
                                                                                                          gggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCg                                                               <  1:665348/126‑1 (MQ=255)
                                                                                                          gggaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCg                                                               >  2:665348/1‑126 (MQ=255)
                                                                                                            gaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCg                                                               <  2:501073/124‑1 (MQ=255)
                                                                                                            gaATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCg                                                               >  1:501073/1‑124 (MQ=255)
                                                                                                             aaTCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATa                                      <  1:404323/148‑1 (MQ=255)
                                                                                                                  aGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAg                                >  3:870763/1‑149 (MQ=255)
                                                                                                                  aGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAg                                >  4:664216/1‑149 (MQ=255)
                                                                                                                   gCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAg                                >  1:708424/1‑148 (MQ=255)
                                                                                                                     aataatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGccc                             >  4:368384/1‑149 (MQ=255)
                                                                                                                     aataatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGccc                             >  4:642024/1‑149 (MQ=255)
                                                                                                                     aataatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGccc                             >  4:576935/1‑149 (MQ=255)
                                                                                                                        aatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCg                                                                                  >  2:381065/1‑93 (MQ=255)
                                                                                                                        aatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCg                                                                                  <  1:381065/93‑1 (MQ=255)
                                                                                                                        aatCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTg                          >  3:848536/1‑149 (MQ=255)
                                                                                                                         atCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTg                          <  1:376586/148‑1 (MQ=255)
                                                                                                                         atCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTg                          <  2:300510/148‑1 (MQ=255)
                                                                                                                         atCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGa                         <  3:653477/149‑1 (MQ=255)
                                                                                                                         atCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGa                         >  3:738226/1‑149 (MQ=255)
                                                                                                                          tCGGGTGTATGGATGTGCAGGTGCCGTCGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGAt                        >  4:568311/1‑149 (MQ=255)
                                                                                                                              gtgtATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTc                    <  4:870763/149‑1 (MQ=255)
                                                                                                                                gtATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCt                  <  4:392079/149‑1 (MQ=255)
                                                                                                                                   tGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTa               <  3:32084/149‑1 (MQ=255)
                                                                                                                                     gATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGc             <  3:576935/149‑1 (MQ=255)
                                                                                                                                         tgCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGtt          >  1:502695/1‑148 (MQ=255)
                                                                                                                                          gCAGGTGCTGACGGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTa         >  2:194269/1‑148 (MQ=255)
                                                                                                                                            aGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTAcc       <  1:731709/148‑1 (MQ=255)
                                                                                                                                                 ccGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTaa  <  2:106890/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGGCAACGTTCGTAGAAAAAACAGCCTTGCGGCAACGTGCGGTTTCCCGGCAGATCCGTTTTACGTAATGCCCATTCTTCCTCCAGCGGTTTATCAATGGCGGGGAGGGAATCCAGCAATAATCGGGTGTATGGATGTGCAGGTGCCGTCAGCACCTGCTGCGCGTCGCCCAGTTCTACAATCTGCCCGAGATACATCACCGCCACCCGATCGCTCATATGACGTATCACCGAGACATTGTGTGAAATCAGCACATAGGTCAGCCCGTGATTTTCCTGTAGCGTTACCAGTAA  >  NC_000913/1557197‑1557489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: