Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,692,979	G→A	5.2%	L429F (CTC→TTC) ‡	uidB ←	glucuronide transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,692,979	0	G	A	5.2%	250.6 / 3.0	77	L429F (CTC→TTC) ‡	uidB	glucuronide transporter
Reads supporting (aligned to +/- strand):  ref base G (43/30);  new base A (2/2);  total (45/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

TGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGA  >  NC_000913/1692833‑1693126                                                                                                                                                   |                                                                                                                                                    tGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCg                                                                                                                                                   <  4:724962/149‑1 (MQ=255)       cTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATaa                                                                                                                                              >  2:91749/1‑148 (MQ=255)         aTTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGCTCACGGATaaaaa                                                                                                                                           >  3:742693/1‑148 (MQ=255)          tttttCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGCTCACGGATaaaaaa                                                                                                                                          <  3:874713/149‑3 (MQ=255)             ttCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGCTCACGGATaaaaaattc                                                                                                                                       <  4:742693/149‑6 (MQ=255)                tcATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGataa                                                                                                                                    >  4:311802/1‑149 (MQ=255)                 cATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGataa                                                                                                                                    >  1:450115/1‑148 (MQ=255)                   caGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGAtaataa                                                                                                                                 <  3:78665/148‑1 (MQ=255)                    aGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGAtaataa                                                                                                                                 >  1:558512/1‑148 (MQ=255)                              ttGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAg                                                                                                                       >  1:903582/1‑148 (MQ=255)                                     tCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGAtaataa                                                                                                                                 <  2:391320/131‑1 (MQ=255)                                     tCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGAtaataa                                                                                                                                 >  1:391320/1‑131 (MQ=255)                                         attattTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATcc                                                                                                           >  3:81020/1‑149 (MQ=255)                                               tttATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAg                                                                                                      >  1:11217/1‑148 (MQ=255)                                               tttATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGCTCACGGATaaaaa                                                                                                                                           >  1:768825/1‑110 (MQ=255)                                                   ttGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACt                                                                                                 >  4:28373/1‑149 (MQ=255)                                                     gAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTa                                                                                                <  2:787250/148‑1 (MQ=255)                                                      aaTATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTaa                                                                                               <  1:405571/148‑1 (MQ=255)                                                      aaTATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTaa                                                                                               <  2:558512/148‑1 (MQ=255)                                                       atatTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAg                                                                                              >  1:190654/1‑148 (MQ=255)                                                        tatTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAgg                                                                                             >  2:792450/1‑148 (MQ=255)                                                        tatTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAgg                                                                                             >  2:656685/1‑148 (MQ=255)                                                               taGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGa                                                                                      <  1:254128/148‑1 (MQ=255)                                                                 gTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGAtgt                                                                                   >  3:858372/1‑149 (MQ=255)                                                                 gTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGAtgt                                                                                   >  4:131847/1‑149 (MQ=255)                                                                     tatCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCg                                                                                >  2:202447/1‑148 (MQ=255)                                                                      atCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCgg                                                                               >  2:153608/1‑148 (MQ=255)                                                                      atCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGa                                                                              >  3:341661/1‑149 (MQ=255)                                                                      atCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGa                                                                              <  3:311802/149‑1 (MQ=255)                                                                         gCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGc                                                                           >  3:749206/1‑149 (MQ=255)                                                                            gATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGccc                                                                         >  1:666967/1‑148 (MQ=255)                                                                             aTTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCat                                                                       >  3:260980/1‑149 (MQ=255)                                                                              ttAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAACCCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGc                                                                           <  4:522163/144‑1 (MQ=255)                                                                              ttAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGc                                                                           >  3:522163/1‑144 (MQ=255)                                                                              ttAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCata                                                                      >  3:121847/1‑149 (MQ=255)                                                                              ttAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCat                                                                       >  2:407457/1‑148 (MQ=255)                                                                              ttAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCat                                                                       >  1:122807/1‑148 (MQ=255)                                                                               tAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCata                                                                      <  2:740349/148‑1 (MQ=255)                                                                                aaTTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCataat                                                                    <  3:131847/149‑1 (MQ=255)                                                                                   tgctgctgcACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCAtaataa                                                                  <  1:656685/148‑1 (MQ=255)                                                                                         tgcACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCgg                                                           >  4:823180/1‑149 (MQ=255)                                                                                          gcACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCgg                                                           >  2:735921/1‑148 (MQ=255)                                                                                              ttttttACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCataa                                                                     <  2:431730/134‑1 (MQ=255)                                                                                              ttttttACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCataa                                                                     >  1:431730/1‑134 (MQ=255)                                                                                              ttttttACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGttt                                                      >  3:751187/1‑149 (MQ=255)                                                                                                 tttACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCa                                                   <  4:858372/149‑1 (MQ=255)                                                                                                  ttACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCa                                                   >  1:296267/1‑148 (MQ=255)                                                                                                      gATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGa                                              >  3:37776/1‑149 (MQ=255)                                                                                                      gATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGa                                              >  4:107894/1‑149 (MQ=255)                                                                                                      gATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGa                                              >  4:622360/1‑149 (MQ=255)                                                                                                        ttATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGAt                                             >  1:915089/1‑148 (MQ=255)                                                                                                            cAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTg                                               <  1:118990/142‑1 (MQ=255)                                                                                                            cAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTg                                               >  2:118990/1‑142 (MQ=255)                                                                                                             aaTTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCg                                        <  1:153608/148‑1 (MQ=255)                                                                                                             aaTTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCg                                        <  2:168117/148‑1 (MQ=255)                                                                                                             aaTTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGa                                       >  3:207944/1‑149 (MQ=255)                                                                                                              aTTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGat                                      >  3:368525/1‑149 (MQ=255)                                                                                                                ttCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACC‑GATTGGCGatat                                    >  1:666964/1‑148 (MQ=255)                                                                                                                    aCCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATcc                                <  4:751187/149‑1 (MQ=255)                                                                                                                    aCCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATc                                 <  2:11217/148‑1 (MQ=255)                                                                                                                      cACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGc                              <  3:622360/149‑1 (MQ=255)                                                                                                                      cACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGc                              >  3:146341/1‑149 (MQ=255)                                                                                                                          aTTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTa                           <  2:752590/148‑1 (MQ=255)                                                                                                                                ttGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCa                     >  2:836888/1‑148 (MQ=255)                                                                                                                                   aaTTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAAt                 >  3:387989/1‑149 (MQ=255)                                                                                                                                      tttttATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATaa               <  2:122807/148‑1 (MQ=255)                                                                                                                                      tttttATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATaa               >  2:808178/1‑148 (MQ=255)                                                                                                                                       ttttATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATaaa              <  1:202447/148‑1 (MQ=255)                                                                                                                                        tttATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAg             <  2:915089/148‑1 (MQ=255)                                                                                                                                          tATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGc           <  1:407457/148‑1 (MQ=255)                                                                                                                                           aTCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAg         <  4:368525/149‑1 (MQ=255)                                                                                                                                           aTCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAg         >  4:745189/1‑149 (MQ=255)                                                                                                                                             ccGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAgg        <  2:903582/148‑1 (MQ=255)                                                                                                                                              cGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGa       <  1:792450/148‑1 (MQ=255)                                                                                                                                               gTGAGCGGATAAAAGCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAAt     >  3:67564/1‑149 (MQ=255)                                                                                                                                               gTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAAt     <  4:341661/149‑1 (MQ=255)                                                                                                                                                tGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAAtt    <  3:28373/149‑1 (MQ=255)                                                                                                                                                   gCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGa  <  1:808178/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    TGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAGGAATTGA  >  NC_000913/1692833‑1693126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑