BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,702,495	C→G	5.8%	A88G (GCC→GGC)	add →	adenosine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,702,495	0	C	G	5.8%	482.5 / 16.6	138	A88G (GCC→GGC)	add	adenosine deaminase
Reads supporting (aligned to +/- strand): ref base C (68/62); new base G (4/4); total (72/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

Only 100 of 140 total aligned reads displayed.

CCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTA  >  NC_000913/1702348‑1702641

   tGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCg                                                                                                                                                 >  2:134368/1‑148 (MQ=255)

         cacTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACgg                                                                                                                                           >  1:754965/1‑148 (MQ=255)

           cTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGcc                                                                                                                                         <  2:892635/148‑1 (MQ=255)

           cTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGcc                                                                                                                                         <  1:362240/148‑1 (MQ=255)

           cTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCt                                                                                                                                        >  4:37654/1‑149 (MQ=255)

             gATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTg                                                                                                                                       >  1:436972/1‑148 (MQ=255)

             gATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCATGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGGCCGTGACGGGCTg                                                                                                                                       >  2:727312/1‑148 (MQ=255)

              aTTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGc                                                                                                                                      >  2:770979/1‑148 (MQ=255)

               ttCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCAc                                                                                                                                    <  4:823977/149‑1 (MQ=255)

                 ccccACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGGCCGTGACGGGCTGCatc                                                                                                                                   <  1:727312/148‑3 (MQ=255)

                   ccACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTAt                                                                                                                                 >  1:502975/1‑148 (MQ=255)

                   ccACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTAt                                                                                                                                 >  2:172386/1‑148 (MQ=255)

                    cACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTg                                                                                                                                       <  2:299/141‑1 (MQ=255)

                    cACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATg                                                                                                                                >  2:305935/1‑148 (MQ=255)

                    cACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTCAAGATGCAGCCCGTCACGGCCTg                                                                                                                                       >  1:299/1‑141 (MQ=255)

                       gTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCg                                                                                                                             <  1:810546/148‑1 (MQ=255)

                         tCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAg                                                                                                                           >  1:97884/1‑148 (MQ=255)

                          cAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTc                                                                                                                              >  4:373971/1‑144 (MQ=255)

                          cAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTc                                                                                                                              <  3:725850/144‑1 (MQ=255)

                             gTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGGCCGTGACGGGCTGCatct                                                                                                                                  >  3:890133/1‑134 (MQ=255)

                               cATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGt                                                                                                                     >  1:509970/1‑148 (MQ=255)

                                aTTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGttt                                                                                                                   <  4:700673/149‑1 (MQ=255)

                                aTTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGttt                                                                                                                   <  4:293494/149‑1 (MQ=255)

                                       aCGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCAcc                                                                                                             <  1:850403/148‑1 (MQ=255)

                                       aCGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCAcc                                                                                                             <  1:770979/148‑1 (MQ=255)

                                       aCGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCAcc                                                                                                             >  2:642132/1‑148 (MQ=255)

                                            cccGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACg                                                                                                                                            <  4:552139/112‑1 (MQ=255)

                                            cccGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACg                                                                                                                                            >  3:552139/1‑112 (MQ=255)

                                              cGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGtttt                                                                                                                  >  1:837074/1‑136 (MQ=255)

                                              cGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGtttt                                                                                                                  <  2:837074/136‑1 (MQ=255)

                                                aTCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACatg                                                                                                   <  3:238615/149‑1 (MQ=255)

                                                    ggTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACatggcaa                                                                                               <  4:597124/149‑1 (MQ=255)

                                                     gTGAACTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACatggcaat                                                                                              >  3:669046/1‑149 (MQ=255)

                                                      tGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACatggcaatg                                                                                             <  4:874748/149‑1 (MQ=255)

                                                         gCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACatggcaatggca                                                                                          <  4:692437/149‑1 (MQ=255)

                                                                gACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAg                                                                                    <  1:352628/148‑1 (MQ=255)

                                                                  ccAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCt                                                                                  >  1:885956/1‑148 (MQ=255)

                                                                   cAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGc                                                                                >  4:863849/1‑149 (MQ=255)

                                                                   cAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGc                                                                                <  3:355990/149‑1 (MQ=255)

                                                                   cAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGc                                                                                >  4:427498/1‑149 (MQ=255)

                                                                   cAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGACTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACatgg                                                                                                  <  4:683297/131‑1 (MQ=255)

                                                                   cAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGACTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACatgg                                                                                                  >  3:683297/1‑131 (MQ=255)

                                                                    aaaCTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTc                                                                                                                                               <  2:420053/85‑1 (MQ=255)

                                                                    aaaCTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTc                                                                                                                                               >  1:420053/1‑85 (MQ=255)

                                                                    aaaCTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGc                                                                                >  1:192324/1‑148 (MQ=255)

                                                                        ttGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTa                                                                           >  3:406429/1‑149 (MQ=255)

                                                                            cTGGCGCGCTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGTCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTTCGTTTTTCACCAGGCTACAGGGCAATGGCACATCAGCTGCCTGTAGCgg                                                                       <  4:345944/149‑1 (MQ=255)

                                                                              ggggCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGgt                                                                     <  4:724485/149‑1 (MQ=255)

                                                                                 gCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGgtgt                                                                   <  2:462711/148‑1 (MQ=255)

                                                                                   gTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGtgttgt                                                                >  4:362485/1‑149 (MQ=255)

                                                                                    ttAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTc                                                               <  3:863849/149‑1 (MQ=255)

                                                                                    ttAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTc                                                               >  3:420251/1‑149 (MQ=255)

                                                                                     ttccgatctcGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCg                                                              <  3:82019/143‑1 (MQ=255)

                                                                                      aaaGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGa                                                             >  4:296299/1‑149 (MQ=255)

                                                                                       aaGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGaa                                                            >  3:17980/1‑149 (MQ=255)

                                                                                           tctcGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCgg                                                        <  4:496964/149‑1 (MQ=255)

                                                                                           tctcGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCg                                                         >  1:787431/1‑148 (MQ=255)

                                                                                            ctcGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTAGATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGt                                                       >  3:766735/1‑149 (MQ=255)

                                                                                            ctcGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGt                                                       >  3:673336/1‑149 (MQ=255)

                                                                                             tcGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGt                                                       <  1:245330/148‑1 (MQ=255)

                                                                                              cGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGa                                                     <  4:17980/149‑1 (MQ=255)

                                                                                               gCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATc                                                                                      >  3:3557/1‑115 (MQ=255)

                                                                                               gCCTCTCTTGAGGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACAAGGCTATATGGCAGTGGCACATCAGCTGCCTGTAGCGGGTTTTGTCGAAgatcggaa                                                    >  4:82019/1‑142 (MQ=255)

                                                                                                ccTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATc                                                   <  4:44749/149‑1 (MQ=255)

                                                                                                  tctctTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGa                                                 >  3:13668/1‑149 (MQ=255)

                                                                                                   ctctTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCGCCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGAt                                                <  4:717719/149‑1 (MQ=255)

                                                                                                   ctctTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGa                                                 <  2:754965/148‑1 (MQ=255)

                                                                                                   ctctTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGAt                                                <  3:654965/149‑1 (MQ=255)

                                                                                                   ctctTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGAGGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGa                                                 <  1:110432/148‑1 (MQ=255)

                                                                                                      ttGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGt                                                                                                                               <  3:461998/67‑1 (MQ=255)

                                                                                                      ttGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATgg                                              >  1:171862/1‑148 (MQ=255)

                                                                                                      ttGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATgg                                              <  1:789129/148‑1 (MQ=255)

                                                                                                       tGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATg                                                                                                                                >  2:387523/1‑65 (MQ=255)

                                                                                                          tGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTAc                                         <  3:216230/149‑1 (MQ=255)

                                                                                                           gCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACg                                        >  4:157205/1‑149 (MQ=255)

                                                                                                           gCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACg                                        >  4:548772/1‑149 (MQ=255)

                                                                                                            ccTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACg                                        >  2:675721/1‑148 (MQ=255)

                                                                                                                tCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAg                                   <  3:101187/149‑1 (MQ=255)

                                                                                                                     gcgTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGc                              <  4:351460/149‑1 (MQ=255)

                                                                                                                      cgTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGc                              <  1:181911/148‑1 (MQ=255)

                                                                                                                       gTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCg                            >  4:751764/1‑149 (MQ=255)

                                                                                                                        tGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGc                           >  4:211659/1‑149 (MQ=255)

                                                                                                                           cATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCAcc                        <  4:203062/149‑1 (MQ=255)

                                                                                                                              ttGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCtt                      >  2:452770/1‑148 (MQ=255)

                                                                                                                                   aaCATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGgtgt                >  4:189038/1‑149 (MQ=255)

                                                                                                                                     cATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGtgtg               >  1:160802/1‑148 (MQ=255)

                                                                                                                                      aTTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGc              <  2:771478/148‑1 (MQ=255)

                                                                                                                                        tGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAgg           <  3:548772/149‑1 (MQ=255)

                                                                                                                                          aaGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGc          >  1:218188/1‑148 (MQ=255)

                                                                                                                                             aTGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGaa       <  2:664942/148‑1 (MQ=255)

                                                                                                                                             aTGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGaa       <  2:171862/148‑1 (MQ=255)

                                                                                                                                              tGCAGGCCGTGACGGGCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATc                                                                                      <  2:402779/68‑1 (MQ=38)

                                                                                                                                              tGCAGGCCGTGACGGGCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATc                                                                                      >  1:402779/1‑68 (MQ=38)

                                                                                                                                              tGCAGGCCGTGACGGGCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAg      >  2:695544/1‑148 (MQ=255)

                                                                                                                                               gCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCt    >  3:19413/1‑149 (MQ=255)

                                                                                                                                               gCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCt    >  3:494036/1‑149 (MQ=255)

                                                                                                                                                 aGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTa  <  4:494036/149‑1 (MQ=255)

                                                                                                                                                  gCCCGTCACGGCTTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTa  <  2:795530/148‑1 (MQ=255)

                                                                                                                                                  gCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGaa                                    >  3:239345/1‑114 (MQ=255)

                                                                                                                                                  gCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGaa                                    <  4:239345/114‑1 (MQ=255)

CCCTGGAAACACTGATTCCCCACGTTCAGGTCATTGCCAACGAACCCGATCTGGTGAGCTTTCTGACCAAACTTGACTGGGGCGTTAAAGTTCTCGCCTCTCTTGATGCCTGTCGCCGCGTGGCATTTGAAAACATTGAAGATGCAGCCCGTCACGGCCTGCACTATGTCGAGCTGCGTTTTTCACCAGGCTACATGGCAATGGCACATCAGCTGCCTGTAGCGGGTGTTGTCGAAGCGGTGATCGATGGCGTACGTGAAGGTTGCCGCACCTTTGGTGTGCAGGCGAAGCTTA  >  NC_000913/1702348‑1702641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`