Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,747,025 G→C 9.3% intergenic (+20/‑106) ydhR → / → ydhS putative monooxygenase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,747,0250GC 9.3% 176.7 / 8.9 54intergenic (+20/‑106)ydhR/ydhSputative monooxygenase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (25/24);  new base C (2/3);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

AGCGCCCTTGCCTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCT  >  NC_000913/1746880‑1747173
                                                                                                                                                 |                                                                                                                                                    
aGCGCCCTTGCCTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGtt                                                                                                                                                    <  1:535633/148‑1 (MQ=255)
       ttGCCTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCt                                                                                                                                            >  4:417432/1‑149 (MQ=255)
       ttGCCTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCt                                                                                                                                            <  3:280487/149‑1 (MQ=255)
        tGCCTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGCTAACCGCCCtt                                                                                                                                           <  4:869135/149‑1 (MQ=255)
          ccTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCttt                                                                                                                                          <  2:778772/148‑1 (MQ=255)
          ccTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGCTAACCGCCCttga                                                                                                                                         <  3:492958/149‑3 (MQ=255)
               cTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATc                                                                                                                                     <  2:680289/148‑1 (MQ=255)
               cTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATc                                                                                                                                     <  1:162393/148‑1 (MQ=255)
               cTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATc                                                                                                                                     <  2:469705/148‑1 (MQ=255)
                     aaaCATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCt                                                                                                                              >  3:397904/1‑149 (MQ=255)
                         aTACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGaaa                                                                                                                          >  3:90137/1‑149 (MQ=255)
                         aTACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGaa                                                                                                                           <  1:704474/148‑1 (MQ=255)
                            cTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAAttt                                                                                                                       <  4:492649/149‑1 (MQ=255)
                                  gACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCaa                                                                                                                  <  1:317963/148‑1 (MQ=255)
                                       aaaaaTCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTa                                                                                                            >  3:418700/1‑149 (MQ=255)
                                         aaaTCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTAGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTaaa                                                                                                          >  3:332423/1‑149 (MQ=255)
                                              tcGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAAtct                                                                                                     >  3:690961/1‑149 (MQ=255)
                                                 gCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCa                                                                                                   <  1:897841/148‑1 (MQ=255)
                                                    ttGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGCTAACCGCCCttg                                                                                                                                          >  2:464698/1‑105 (MQ=255)
                                                    ttGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGCTAACCGCCCttg                                                                                                                                          <  1:464698/106‑2 (MQ=255)
                                                     tGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCa                                                                                               >  1:4451/1‑148 (MQ=255)
                                                      gAGGAAGTTGTCGCCGAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCaaa                                                                                             >  3:336382/1‑149 (MQ=255)
                                                             ttGTCGCCAAAGTTTTCTTTGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCt                                                                                      <  4:332423/149‑1 (MQ=255)
                                                                  gCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATaa                                                                                 <  3:629726/149‑1 (MQ=255)
                                                                              gATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGa                                                                     <  3:161563/149‑1 (MQ=255)
                                                                                tGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGaaa                                                                   <  4:397904/149‑1 (MQ=255)
                                                                                   cAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGaa                                                                <  3:640067/149‑1 (MQ=255)
                                                                                           cACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGa                                                                                    <  1:103136/121‑1 (MQ=255)
                                                                                           cACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGa                                                                                    >  2:103136/1‑121 (MQ=255)
                                                                                           cACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTaa                                                                           >  2:861721/1‑130 (MQ=255)
                                                                                           cACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTaa                                                                           <  1:861721/130‑1 (MQ=255)
                                                                                           cACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACa                                                        >  3:83666/1‑149 (MQ=255)
                                                                                              ttAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgt                                                     >  4:88186/1‑149 (MQ=255)
                                                                                                aGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgtaa                                                   <  3:417432/149‑1 (MQ=255)
                                                                                                 gTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGaa                                                                    >  3:822858/1‑131 (MQ=255)
                                                                                                 gTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGaa                                                                    <  4:822858/131‑1 (MQ=255)
                                                                                                  tCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgtaagt                                                 >  3:579211/1‑149 (MQ=255)
                                                                                                  tCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgtaag                                                  >  1:39657/1‑148 (MQ=255)
                                                                                                    aaaTCAATCAGGCTAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGgtaagtaa                                               <  4:418700/149‑1 (MQ=255)
                                                                                                       tcaatcaGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACaa                                            <  3:396157/149‑1 (MQ=255)
                                                                                                                 aaaaCTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAAt                                  >  4:636577/1‑149 (MQ=255)
                                                                                                                  aaaCTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAAtt                                 <  4:18858/149‑1 (MQ=255)
                                                                                                                      tCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAAt                                           <  3:839532/135‑1 (MQ=255)
                                                                                                                      tCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAAt                                           >  4:839532/1‑135 (MQ=255)
                                                                                                                       cGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTa                             >  2:259975/1‑148 (MQ=255)
                                                                                                                                 aaTTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATCCTAAAAATCTCCACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGc                   >  2:531649/1‑148 (MQ=255)
                                                                                                                                   tttAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCgg                >  3:253426/1‑149 (MQ=255)
                                                                                                                                   tttAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCg                 >  1:161191/1‑148 (MQ=255)
                                                                                                                                   tttAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCCGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCg                 >  1:458922/1‑148 (MQ=255)
                                                                                                                                         cAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTa           >  1:86046/1‑148 (MQ=255)
                                                                                                                                         cAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTa           >  1:636192/1‑148 (MQ=255)
                                                                                                                                           aGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACg         >  2:136380/1‑148 (MQ=255)
                                                                                                                                           aGGGCGCTAACCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACg         >  2:789836/1‑148 (MQ=255)
                                                                                                                                              gCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGa     <  4:90137/149‑1 (MQ=255)
                                                                                                                                                ggTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGAt    <  2:4451/148‑1 (MQ=255)
                                                                                                                                                ggTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATc   <  4:690961/149‑1 (MQ=255)
                                                                                                                                                 gTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCt  <  4:253426/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
AGCGCCCTTGCCTATCTGGAAAAACATACTGCCCGACTGAAAAATCTCGGCGTTGAGGAAGTTGTCGCCAAAGTTTTCGATGTCAATGAGCCACTTAGTCAAATCAATCAGGCAAAACTCGCCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCT  >  NC_000913/1746880‑1747173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑