Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,810,069 G→A 8.0% intergenic (+21/‑142) yniC → / → ydjM 2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,810,0690GA 8.0% 271.9 / 11.7 87intergenic (+21/‑142)yniC/ydjM2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA
Reads supporting (aligned to +/- strand):  ref base G (33/47);  new base A (4/3);  total (37/50)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

AAGCAGCCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTATCA  >  NC_000913/1809924‑1810211
                                                                                                                                                 |                                                                                                                                              
aaGCAGCCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTg                                                                                                                                             >  4:894447/1‑149 (MQ=255)
 agcagcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGc                                                                                                                                            >  4:834311/1‑149 (MQ=255)
 agcagcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGc                                                                                                                                            >  3:413239/1‑149 (MQ=255)
  gcagcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGc                                                                                                                                            >  1:482560/1‑148 (MQ=255)
    agcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGccc                                                                                                                                          <  2:760761/148‑1 (MQ=255)
     gcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGcccc                                                                                                                                         <  1:755422/148‑1 (MQ=255)
     gcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGcccc                                                                                                                                         <  1:4478/148‑1 (MQ=255)
      cccGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGCGAGATCACTGCCCCTg                                                                                                                                       <  3:405864/149‑1 (MQ=255)
         gCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGt                                                                                                                                     <  1:200436/148‑1 (MQ=255)
           aTGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTc                                                                                                                                   <  1:604147/148‑1 (MQ=255)
           aTGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTc                                                                                                                                   <  1:723540/148‑1 (MQ=255)
             gCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCtt                                                                                                                                 <  2:862679/148‑1 (MQ=255)
               gTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTAt                                                                                                                              >  4:212690/1‑149 (MQ=255)
                ttCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTAt                                                                                                                              >  2:526846/1‑148 (MQ=255)
                ttCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATc                                                                                                                             <  3:549303/149‑1 (MQ=255)
                   cATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTg                                                                                                                           <  1:893544/148‑1 (MQ=255)
                     tcgtcgTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGcc                                                                                                                                           >  2:864715/1‑130 (MQ=255)
                     tcgtcgTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGcc                                                                                                                                           <  1:864715/130‑1 (MQ=255)
                     tcgtcgTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGaa                                                                                                                         <  2:482560/148‑1 (MQ=255)
                     tcgtcgTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGaa                                                                                                                         >  1:258338/1‑148 (MQ=255)
                     tcgtcgTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGaa                                                                                                                         <  1:830026/148‑1 (MQ=255)
                        tcgtTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTGATGACCAGGGGCAGCGAGATCACTGCCCCTg                                                                                                                                       <  1:775768/131‑1 (MQ=255)
                        tcgtTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGCGAGATCACTGCCCCTg                                                                                                                                       >  2:775768/1‑131 (MQ=255)
                          gtTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCa                                                                                                                   >  3:358184/1‑149 (MQ=255)
                          gtTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCa                                                                                                                   <  3:834311/149‑1 (MQ=255)
                            tCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCAtt                                                                                                                 <  4:413239/149‑1 (MQ=255)
                             ccTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCAtt                                                                                                                 <  2:451128/148‑1 (MQ=255)
                                       aaGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGa                                                                                                      >  4:378120/1‑149 (MQ=255)
                                          cgcAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGAtt                                                                                                    <  1:814817/148‑1 (MQ=255)
                                            cAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAAt                                                                                                 <  3:212690/149‑1 (MQ=255)
                                             aaaaTGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAAt                                                                                                 <  1:302714/148‑1 (MQ=255)
                                             aaaaTGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATc                                                                                                >  4:561525/1‑149 (MQ=255)
                                              aaaTGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATc                                                                                                >  1:254345/1‑148 (MQ=255)
                                              aaaTGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCg                                                                                               <  4:699386/149‑1 (MQ=255)
                                               aaTGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCg                                                                                               <  1:666706/148‑1 (MQ=255)
                                                       aCGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTaa                                                                                      <  4:407314/149‑1 (MQ=255)
                                                       aCGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTaa                                                                                      <  4:358184/149‑1 (MQ=255)
                                                       aCGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTa                                                                                       <  1:193815/148‑1 (MQ=255)
                                                       aCGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACCCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTaa                                                                                      <  3:842989/149‑1 (MQ=255)
                                                                aTTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAaga                                                                              >  1:698991/1‑148 (MQ=255)
                                                                 ttAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGt                                                                            <  3:174721/149‑1 (MQ=255)
                                                                    gCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTa                                                                          <  2:335502/148‑1 (MQ=255)
                                                                     cAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTaca                                                                        <  3:668160/149‑1 (MQ=255)
                                                                           tCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTca                                                                  <  4:49687/149‑1 (MQ=255)
                                                                             aaaCTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTaa                                                                                      <  3:403246/127‑1 (MQ=255)
                                                                             aaaCTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTaa                                                                                      >  4:403246/1‑127 (MQ=255)
                                                                             aaaCTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTcacc                                                                >  3:727120/1‑149 (MQ=255)
                                                                                cTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACt                                                             >  4:31052/1‑149 (MQ=255)
                                                                                  ttCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACtt                                                            >  1:432677/1‑148 (MQ=255)
                                                                                     aTCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCg                                                         <  1:526846/148‑1 (MQ=255)
                                                                                      tCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGa                                                                                                          <  4:122349/98‑1 (MQ=255)
                                                                                      tCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGa                                                                                                          >  3:122349/1‑98 (MQ=255)
                                                                                       cGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTg                                                       >  1:558772/1‑148 (MQ=255)
                                                                                         cTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCa                                                     <  2:558772/148‑1 (MQ=255)
                                                                                           gACAGAACTCAGCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTg                                                  >  3:514502/1‑149 (MQ=255)
                                                                                             cAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAAc                                                                                                             >  1:568039/1‑88 (MQ=255)
                                                                                             cAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAAc                                                                                                             <  2:568039/88‑1 (MQ=255)
                                                                                                 aCTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAAt                                                                                                 <  2:724064/96‑1 (MQ=255)
                                                                                                 aCTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAAt                                                                                                 >  1:724064/1‑96 (MQ=255)
                                                                                                 aCTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTa                                                                                       >  1:456234/1‑106 (MQ=255)
                                                                                                 aCTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTa                                                                                       <  2:456234/106‑1 (MQ=255)
                                                                                                   tCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGGTACACCGTCACCACTTCCGTGCACTGTATaaaaa                                          >  4:282125/1‑149 (MQ=255)
                                                                                                    cACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaaaa                                          <  2:406133/148‑1 (MQ=255)
                                                                                                    cACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATaaaaa                                          >  2:298346/1‑148 (MQ=255)
                                                                                                     aCCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAAt                                         <  2:254345/148‑1 (MQ=255)
                                                                                                       cGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTaa                                                                                                  <  4:551323/89‑1 (MQ=255)
                                                                                                       cGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTaa                                                                                                  >  3:551323/1‑89 (MQ=255)
                                                                                                        gCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCta                                     <  3:378120/149‑1 (MQ=255)
                                                                                                         cAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCta                                     >  1:903484/1‑148 (MQ=255)
                                                                                                          aaaaGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCtata                                   <  3:31052/149‑1 (MQ=255)
                                                                                                           aaaGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATAc                                  >  4:837974/1‑149 (MQ=255)
                                                                                                              gACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTg                                >  2:592127/1‑148 (MQ=255)
                                                                                                                ccTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTa                              <  2:698991/148‑1 (MQ=255)
                                                                                                                    ctcGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTAt                         <  3:894447/149‑1 (MQ=255)
                                                                                                                     tcGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCAc                                                    <  3:729328/121‑1 (MQ=255)
                                                                                                                     tcGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCAc                                                    >  4:729328/1‑121 (MQ=255)
                                                                                                                            aTGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGtt                 <  3:837974/149‑1 (MQ=255)
                                                                                                                              gACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGttt                <  2:432677/148‑1 (MQ=255)
                                                                                                                               aCCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTa               <  1:592127/148‑1 (MQ=255)
                                                                                                                                ccAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATc                                        <  1:335207/122‑1 (MQ=255)
                                                                                                                                ccAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATc                                        >  2:335207/1‑122 (MQ=255)
                                                                                                                                 cAGGGGCAGCGAGATCACTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTg            >  3:351263/1‑149 (MQ=255)
                                                                                                                                   ggggCAGCGAGATCACTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCg                                                         <  1:337981/102‑1 (MQ=39)
                                                                                                                                   ggggCAGCGAGATCACTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCg                                                         >  2:337981/1‑102 (MQ=39)
                                                                                                                                   ggggCAGCGAGATCACTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAg          >  4:319105/1‑149 (MQ=255)
                                                                                                                                     ggCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGt         >  1:282284/1‑148 (MQ=255)
                                                                                                                                           gATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTATCa  >  3:630733/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
AAGCAGCCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAGTGATCTCGCTGCCCCTGGTTCTTTATCTGAAATTGCATTCAACTGACGGATTAATCGTCAATTTAAGAGAAAGAGTTACACCGTCACCACTTCCGTGCACTGTATAAAAATCCTATACTGTACGTATCGACAGTTTAGTGAGTTTTATCA  >  NC_000913/1809924‑1810211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: