Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,906,231 C→A 7.2% L182I (CTC→ATC)  mntP → putative Mn(2+) efflux pump, mntR‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,906,2310CA 7.2% 282.2 / 10.0 85L182I (CTC→ATC) mntPputative Mn(2+) efflux pump, mntR‑regulated
Reads supporting (aligned to +/- strand):  ref base C (34/43);  new base A (3/3);  total (39/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

TTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCG  >  NC_000913/1906085‑1906377
                                                                                                                                                   |                                                                                                                                                  
ttATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCctc                                                                                                                                                  <  3:164296/149‑1 (MQ=255)
ttATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCctc                                                                                                                                                  >  4:849227/1‑149 (MQ=255)
ttATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCct                                                                                                                                                   >  2:316400/1‑148 (MQ=255)
ttATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCct                                                                                                                                                   >  1:398790/1‑148 (MQ=255)
    cgcgACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGa                                                                                                                                              >  4:630217/1‑149 (MQ=255)
     gcgACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGAc                                                                                                                                             >  3:166779/1‑149 (MQ=255)
       gACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGAGGATCTGGACg                                                                                                                                            <  1:480476/148‑1 (MQ=255)
         ccGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCAc                                                                                                                                         <  4:653808/149‑1 (MQ=255)
         ccGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCAc                                                                                                                                         >  3:133561/1‑149 (MQ=255)
                 gCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTCTATCGTCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGTCGTCCAGATCTTCTGGACGCACTTCCACg                                                                                                                                  >  2:102570/1‑148 (MQ=255)
                    aTTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTta                                                                                                                              <  4:23761/149‑1 (MQ=255)
                    aTTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGAGGATCTGGAc                                                                                                                                             >  3:401867/1‑134 (MQ=255)
                     ttGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTtaa                                                                                                                             <  4:166779/149‑1 (MQ=255)
                     ttGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTta                                                                                                                              <  1:474967/148‑1 (MQ=255)
                      tGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCctc                                                                                                                                                  <  1:799924/127‑1 (MQ=255)
                      tGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCctc                                                                                                                                                  >  2:799924/1‑127 (MQ=255)
                        gTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTtaataa                                                                                                                          <  4:735078/149‑1 (MQ=255)
                        gTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTtaataa                                                                                                                          >  3:316621/1‑149 (MQ=255)
                           gtgCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGaa                                                                                                                       <  3:482294/149‑1 (MQ=255)
                           gtgCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGaa                                                                                                                       <  3:443003/149‑1 (MQ=255)
                              cAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACgg                                                                                                                                 >  2:174195/1‑136 (MQ=255)
                              cAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACgg                                                                                                                                 <  1:174195/136‑1 (MQ=255)
                              cAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACg                                                                                                                     <  1:569371/148‑1 (MQ=255)
                               aaCCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTGTGGACGCACTTCCACGGTtaataa                                                                                                                          >  3:67500/1‑142 (MQ=255)
                               aaCCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTtaataa                                                                                                                          <  4:67500/142‑1 (MQ=255)
                               aaCCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCt                                                                                                                   <  3:549425/149‑1 (MQ=255)
                                   ttGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCctc                                                                                                                                                  >  3:551288/1‑114 (MQ=255)
                                   ttGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCctc                                                                                                                                                  <  4:551288/114‑1 (MQ=255)
                                   ttGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCa                                                                                                               <  3:849227/149‑1 (MQ=255)
                                   ttGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCa                                                                                                               >  3:711426/1‑149 (MQ=255)
                                      aTTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCaga                                                                                                             >  2:399139/1‑148 (MQ=255)
                                         aTGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGt                                                                                                          >  2:239720/1‑148 (MQ=255)
                                           gTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGaa                                                                                                       <  4:563716/149‑1 (MQ=255)
                                           gTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGaa                                                                                                       <  4:223892/149‑1 (MQ=255)
                                           gTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGa                                                                                                        <  1:755017/148‑1 (MQ=255)
                                            tCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGaa                                                                                                       >  1:640641/1‑148 (MQ=255)
                                            tCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGaa                                                                                                       >  1:364813/1‑148 (MQ=255)
                                             cAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGa                                                                                                        <  2:355122/146‑1 (MQ=255)
                                             cAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGa                                                                                                        >  1:355122/1‑146 (MQ=255)
                                              aaCATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAatat                                                                                                    >  4:552666/1‑149 (MQ=255)
                                                 aTTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTaa                                                                                                 >  4:772413/1‑149 (MQ=255)
                                                       gatgatGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCctc                                                                                                                                                  <  4:228380/94‑1 (MQ=255)
                                                       gatgatGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCctc                                                                                                                                                  >  3:228380/1‑94 (MQ=255)
                                                       gatgatGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTaaa                                                                                                >  4:99704/1‑144 (MQ=255)
                                                       gatgatGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTaaa                                                                                                <  3:99704/144‑1 (MQ=255)
                                                        atgatgGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCt                                                                                                                   >  3:897408/1‑124 (MQ=255)
                                                        atgatgGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCt                                                                                                                   <  4:897408/124‑1 (MQ=255)
                                                        atgatgGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTaaa                                                                                                >  3:559049/1‑143 (MQ=255)
                                                        atgatgGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTaaa                                                                                                <  4:559049/143‑1 (MQ=255)
                                                               ttggtCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCa                                                                                    >  1:726387/1‑148 (MQ=255)
                                                                 ggtCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGt                                                                                  <  2:203862/148‑1 (MQ=255)
                                                                 ggtCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGt                                                                                  <  1:755469/148‑1 (MQ=255)
                                                                    cGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGa                                                                               >  2:354938/1‑148 (MQ=255)
                                                                     gCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGaa                                                                              <  1:683211/148‑1 (MQ=255)
                                                                           tCGGCTCAATTATTGGGAAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACActt                                                                         <  1:12088/148‑1 (MQ=255)
                                                                            cGGCTCAATTATTGGGAAAAAAGCGGAAATACTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGACGAACActtctt                                                                      <  4:433248/149‑1 (MQ=255)
                                                                              gCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCttt                                                                     <  1:239720/148‑1 (MQ=255)
                                                                                tCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTtgc                                                                  >  4:393776/1‑149 (MQ=255)
                                                                                 cAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTtgc                                                                  >  2:500088/1‑148 (MQ=255)
                                                                                          ggAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGtt                                                        >  3:177962/1‑149 (MQ=255)
                                                                                             aaaaaaGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGttt                                                       <  1:399139/148‑1 (MQ=255)
                                                                                              aaaaaGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTg                                                      <  1:702778/148‑1 (MQ=255)
                                                                                              aaaaaGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTg                                                      <  1:770705/148‑1 (MQ=255)
                                                                                              aaaaaGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTg                                                      >  2:823298/1‑148 (MQ=255)
                                                                                              aaaaaGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGc                                                     <  3:190278/149‑1 (MQ=255)
                                                                                               aaaaGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGc                                                     <  2:568896/148‑1 (MQ=255)
                                                                                                aaaGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGcc                                                    >  2:85014/1‑148 (MQ=255)
                                                                                                   gCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGa                                                 <  2:408790/148‑1 (MQ=255)
                                                                                                      gAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTc                                             <  3:772413/149‑1 (MQ=255)
                                                                                                       aaaTTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTc                                             <  2:698156/148‑1 (MQ=255)
                                                                                                        aaTTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGt                                                                                              >  2:351398/1‑98 (MQ=255)
                                                                                                        aaTTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGt                                                                                              <  1:351398/98‑1 (MQ=255)
                                                                                                        aaTTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTc                                                                                 >  3:754632/1‑111 (MQ=255)
                                                                                                        aaTTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTc                                                                                 <  4:754632/111‑1 (MQ=255)
                                                                                                         aTTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTg                                           <  2:398790/148‑1 (MQ=255)
                                                                                                            ctcGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCtt                                       <  4:133561/149‑1 (MQ=255)
                                                                                                                       ggTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACTCTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGTCTTCGCACGCCAg                             <  2:257937/148‑1 (MQ=255)
                                                                                                                               tcggcatcggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACgg                     >  1:456082/1‑148 (MQ=255)
                                                                                                                               tcggcatcggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACgg                     <  2:456082/148‑1 (MQ=255)
                                                                                                                               tcggcatcggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACgg                     <  1:85014/148‑1 (MQ=255)
                                                                                                                                cggcatcggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGc                    >  1:630834/1‑148 (MQ=255)
                                                                                                                                   catcggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTc                 <  2:726387/148‑1 (MQ=255)
                                                                                                                                         cGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGc           >  1:459306/1‑148 (MQ=255)
                                                                                                                                         cGTCCAGAGGATCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGc                                                     >  4:361194/1‑106 (MQ=255)
                                                                                                                                         cGTCCAGAGGATCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGc                                                     <  3:361194/106‑1 (MQ=255)
                                                                                                                                          gTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCa          <  1:102570/148‑1 (MQ=255)
                                                                                                                                          gTCCAGAGGATCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCt                                            >  4:265703/1‑114 (MQ=255)
                                                                                                                                          gTCCAGAGGATCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCt                                            <  3:265703/114‑1 (MQ=255)
                                                                                                                                              aGATCTTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCaataa      >  2:477360/1‑148 (MQ=255)
                                                                                                                                              aGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCaataa      >  1:145933/1‑148 (MQ=255)
                                                                                                                                               gATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATg    >  3:15558/1‑149 (MQ=255)
                                                                                                                                                aTCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGcc                                                    <  4:607909/100‑1 (MQ=255)
                                                                                                                                                aTCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGcc                                                    >  3:607909/1‑100 (MQ=255)
                                                                                                                                                aTCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATg    >  2:717737/1‑148 (MQ=255)
                                                                                                                                                 tCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGc   >  2:324376/1‑148 (MQ=255)
                                                                                                                                                  ccTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCg  >  2:679962/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATGCG  >  NC_000913/1906085‑1906377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: