Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,910,295	A→T	6.8%	D7V (GAC→GTC)	yebQ →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,910,295	0	A	T	6.8%	204.2 / 4.9	59	D7V (GAC→GTC)	yebQ	putative transporter
Reads supporting (aligned to +/- strand):  ref base A (29/26);  new base T (2/2);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

TTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGG  >  NC_000913/1910153‑1910440                                                                                                                                               |                                                                                                                                                  tttCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGTCGGCCt                                                                                                                                             >  3:440310/1‑149 (MQ=255)    cGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGTCGGCCTgaa                                                                                                                                          <  4:440310/149‑3 (MQ=255)      cACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCa                                                                                                                                         <  2:623196/148‑1 (MQ=255)      cACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCa                                                                                                                                         >  1:85416/1‑148 (MQ=255)                  cGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGa                                                                                                                             <  1:633487/148‑1 (MQ=255)                      aTCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACg                                                                                                                         >  1:265957/1‑148 (MQ=255)                         cATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGTCGGCCTgaacttt                                                                                                                                      >  4:874630/1‑126 (MQ=255)                         cATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGTCGGCCTgaacttt                                                                                                                                      <  3:874630/132‑7 (MQ=255)                         cATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTg                                                                                                                      >  1:461691/1‑148 (MQ=255)                          aTCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCg                                                                                                                    >  4:152859/1‑149 (MQ=255)                           tCTTGTGCCGGTTAGTTACAATTCTGCGACATGCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCg                                                                                                                    >  1:258879/1‑148 (MQ=255)                             ttttGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGata                                                                                                                 >  4:106596/1‑149 (MQ=255)                             ttttGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGat                                                                                                                  <  2:85416/148‑1 (MQ=255)                                             cAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGAtt                                                                                                  <  1:899097/148‑1 (MQ=255)                                              aaTTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTgg                                                                                                >  4:262275/1‑149 (MQ=255)                                                      gACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCg                                                                                         >  1:545417/1‑148 (MQ=255)                                                       aCATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGa                                                                                        <  2:150126/148‑1 (MQ=255)                                                       aCATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGa                                                                                        <  1:434965/148‑1 (MQ=255)                                                       aCATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGAt                                                                                       <  3:889819/149‑1 (MQ=255)                                                       aCATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGAt                                                                                       <  3:370600/149‑1 (MQ=255)                                                        cATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGAt                                                                                       <  2:545417/148‑1 (MQ=255)                                                               cGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTcc                                                                               >  4:527807/1‑149 (MQ=255)                                                               cGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTc                                                                                <  2:258879/148‑1 (MQ=255)                                                               cGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATACAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTc                                                                                <  2:461691/148‑1 (MQ=255)                                                                gTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTg                                                                                                                                      >  1:229004/1‑93 (MQ=255)                                                                gTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTg                                                                                                                                      <  2:229004/93‑1 (MQ=255)                                                                gTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTcc                                                                               >  1:322530/1‑148 (MQ=255)                                                                gTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCt                                                                              <  3:262275/149‑1 (MQ=255)                                                                 tGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCtt                                                                             <  4:236228/149‑1 (MQ=255)                                                                 tGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCt                                                                              >  2:687348/1‑148 (MQ=255)                                                                   aaTATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTg                                                                            >  2:594892/1‑148 (MQ=255)                                                                              tAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCg                                                                >  3:181857/1‑149 (MQ=255)                                                                                gAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGc                                                               >  1:712976/1‑148 (MQ=255)                                                                                      tACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTc                                                        <  3:106596/149‑1 (MQ=255)                                                                                           cTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGccc                                                    >  2:293613/1‑148 (MQ=255)                                                                                              ttGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGAt                                                                                       <  4:397066/110‑1 (MQ=255)                                                                                              ttGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGAt                                                                                       >  3:397066/1‑110 (MQ=255)                                                                                                gATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCca                                               >  2:139779/1‑148 (MQ=255)                                                                                                         ccAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTAttt                                                                                           <  1:114490/95‑1 (MQ=255)                                                                                                         ccAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTAttt                                                                                           >  2:114490/1‑95 (MQ=255)                                                                                                          cAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCTACAATCGCCAc                                    <  3:527807/149‑1 (MQ=255)                                                                                                          cAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCa                                     <  2:322530/148‑1 (MQ=255)                                                                                                          cAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCAc                                    >  4:279216/1‑149 (MQ=255)                                                                                                            aaGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACg                                   >  1:823954/1‑148 (MQ=255)                                                                                                             aGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCa                                                             >  2:56601/1‑121 (MQ=255)                                                                                                             aGATATAAAATGCCTATGCCAAAAGTTCAGGCCGACGTCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCa                                                             <  1:56601/121‑1 (MQ=255)                                                                                                                  taAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTc                            >  3:827729/1‑149 (MQ=255)                                                                                                                       ttCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGcc                       <  4:827729/149‑1 (MQ=255)                                                                                                                         ccTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCAc                                    >  2:78681/1‑134 (MQ=255)                                                                                                                         ccTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCAc                                    <  1:78681/134‑1 (MQ=255)                                                                                                                         ccTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCa                      <  1:594892/148‑1 (MQ=255)                                                                                                                                cAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTAtt                                                                                            >  3:469727/1‑71 (MQ=255)                                                                                                                                cAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTAtt                                                                                            <  4:469727/71‑1 (MQ=255)                                                                                                                                 aaaaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACgccagcc              >  1:313909/1‑148 (MQ=255)                                                                                                                                  aaaGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGccagccag            >  4:849299/1‑149 (MQ=255)                                                                                                                                     gTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGtt          <  1:139779/148‑1 (MQ=255)                                                                                                                                          ggCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCt    <  4:181857/149‑1 (MQ=255)                                                                                                                                            ccGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTg   >  1:789240/1‑148 (MQ=255)                                                                                                                                             cGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTgg  >  1:136914/1‑148 (MQ=255)                                                                                                                                               |                                                                                                                                                  TTTCGCACCAGGGTGGTCGCAATCCATCTTTTGCCGGTTAGTTACAATTCTGCGACATCCACCGTGAATATCAGTGCTAGAATCATACCCCTGTTGATTATTCACCAAAGATATAAAATTCCTATGCCAAAAGTTCAGGCCGACGGCCTGCCATTGCCCCAGCGATACGGTGCGATATTAACCATTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGG  >  NC_000913/1910153‑1910440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑