Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,932,095 | T→A | 7.0% | intergenic (+36/+20) | purT → / ← eda | phosphoribosylglycinamide formyltransferase 2/KHG/KDPG aldolase; 2‑dehydro‑3‑deoxy‑phosphogluconate/4‑hydroxy‑2‑ oxoglutarate aldolase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,932,095 | 0 | T | A | 7.0% | 195.6 / 3.4 | 56 | intergenic (+36/+20) | purT/eda | phosphoribosylglycinamide formyltransferase 2/KHG/KDPG aldolase; 2‑dehydro‑3‑deoxy‑phosphogluconate/4‑hydroxy‑2‑ oxoglutarate aldolase |
Reads supporting (aligned to +/- strand): ref base T (30/22); new base A (2/2); total (32/24) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACC‑GATGCACAGCACGCTTTTCAGCGCCAGGT > NC_000913/1931951‑1932242 | aaTTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCTCGATCCTCGAt < 3:513460/149‑1 (MQ=255) gATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCgg > 2:789840/1‑148 (MQ=255) aTGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCggg < 1:789840/148‑1 (MQ=255) cAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAttt < 4:723746/149‑1 (MQ=255) gCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCGGGCAtttt < 1:141570/148‑1 (MQ=255) gCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAtttt < 2:259688/148‑1 (MQ=255) ggggTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGa < 4:45499/139‑1 (MQ=255) ggggTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGa > 3:45499/1‑139 (MQ=255) cTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGcc < 2:636336/148‑1 (MQ=255) aCTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACtttt < 1:871717/129‑1 (MQ=255) aCTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACtttt > 2:871717/1‑129 (MQ=255) agagagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTc < 4:903355/149‑1 (MQ=255) agTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCAcgc < 3:11017/149‑1 (MQ=255) tgttgtTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACgcgc < 3:891598/149‑1 (MQ=255) tgttgtTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACgcgc > 4:629293/1‑149 (MQ=255) tgttgtTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACgcgc > 3:168292/1‑149 (MQ=255) gttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGc > 3:823432/1‑149 (MQ=255) ttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCt > 3:50805/1‑149 (MQ=255) gACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTa < 4:386832/149‑1 (MQ=255) cGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAg > 2:327154/1‑148 (MQ=255) ttGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑g < 1:279003/148‑1 (MQ=255) tGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑Gc > 2:415881/1‑148 (MQ=255) tGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑Gc > 2:140925/1‑148 (MQ=255) aCGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTc > 4:371805/1‑149 (MQ=255) aCGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTc > 3:424458/1‑149 (MQ=255) gcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTaa > 4:654042/1‑149 (MQ=255) aaGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCg > 3:387427/1‑149 (MQ=255) gCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGcc < 4:168292/149‑1 (MQ=255) aCGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCg > 3:186763/1‑149 (MQ=255) cgccgGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTc < 4:186763/149‑1 (MQ=255) gACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAgc > 2:866947/1‑148 (MQ=255) gTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCt > 3:579177/1‑149 (MQ=255) tAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCt > 2:860718/1‑148 (MQ=255) tAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTg > 3:251551/1‑149 (MQ=255) aaGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGcc > 1:543025/1‑148 (MQ=255) aaGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCg < 4:579177/149‑1 (MQ=255) aaGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCg < 4:823432/149‑1 (MQ=255) gTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGcc > 2:764200/1‑127 (MQ=255) gTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGcc < 1:764200/127‑1 (MQ=255) aGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAcca < 4:50805/149‑1 (MQ=255) aGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAcca > 3:890856/1‑149 (MQ=255) gggTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAcca > 2:840915/1‑148 (MQ=255) cccGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGaa > 1:688905/1‑148 (MQ=255) aaaaaCGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAacca > 1:310397/1‑143 (MQ=255) aaaaaCGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAacca < 2:310397/143‑1 (MQ=255) aaCGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACC‑GATGca > 2:501746/1‑148 (MQ=255) cGCTACAAAAATGCCCGATCCTCGATCGGGCATTTGGCCTTTTCCAGC‑TAACGCCCTCCAAAACCTTCCGCGCCAACCTTATAATGGGGGCCGAATTCGCCCGCTTCCAGCCCATCTGCCGG‑ACCAGCCCGGGAACACCGGATGcaca > 1:913168/1‑148 (MQ=255) aaaaTGCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACC‑GATGCACAGCACGCt > 2:712690/1‑148 (MQ=255) aaaaTGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACC‑GATGCACAGCACGCt < 1:415881/148‑1 (MQ=255) aaaaTGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACC‑GATGCACAGCACGCt < 1:327154/148‑1 (MQ=255) tGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACC‑GATGCACAGCACGCTTTTCa > 3:795560/1‑149 (MQ=255) cccGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACC‑GATGCACAGCACGCTTTTCAg < 1:741624/148‑1 (MQ=255) cGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACC‑GATGCACAGCACGCTTTTCAgcg > 2:35241/1‑148 (MQ=255) gATCCTCGATCGGGCTTTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACC‑GATGCACAGCACGCTTTTCAGCGcc > 4:626122/1‑149 (MQ=255) aTCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACC‑GATGCACAGCACGCTTTTCAGCGcc < 2:688905/148‑1 (MQ=255) cTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACC‑GATGCATAGCACGCTTTTCAGCGCCAGGt > 3:555031/1‑149 (MQ=255) tCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACC‑GATg > 2:289057/1‑123 (MQ=255) tCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACC‑GATg < 1:289057/123‑1 (MQ=255) tCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACC‑GATGCACAGCACGCTTTTCAGCGCCAGGt < 1:860718/148‑1 (MQ=255) | AATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT‑GCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACC‑GATGCACAGCACGCTTTTCAGCGCCAGGT > NC_000913/1931951‑1932242 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |