Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,093,969 T→A 6.9% Y168N (TAC→AAC)  hisB → histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,093,9690TA 6.9% 356.6 / 12.5 102Y168N (TAC→AAC) hisBhistidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (40/55);  new base A (4/3);  total (44/58)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 104 total aligned reads displayed.

AGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCC  >  NC_000913/2093821‑2094110
                                                                                                                                                    |                                                                                                                                             
aGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGtt                                                                                                                                               >  3:479396/1‑149 (MQ=255)
 gCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGtt                                                                                                                                               <  2:430385/148‑1 (MQ=255)
 gCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTa                                                                                                                                              >  4:255398/1‑149 (MQ=255)
    aTGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACg                                                                                                                                            <  1:838613/148‑1 (MQ=255)
        aTCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGGCCGCGACACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCa                                                                                                                                        <  2:743959/148‑1 (MQ=255)
                   aGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTaa                                                                                                                            <  3:682750/149‑1 (MQ=255)
                    gTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTaa                                                                                                                            >  1:273678/1‑148 (MQ=255)
                    gTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTaa                                                                                                                            <  2:293436/148‑1 (MQ=255)
                     ttATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAGCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATa                                                                                                                          <  3:309209/149‑1 (MQ=255)
                     ttATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATa                                                                                                                          <  3:554367/149‑1 (MQ=255)
                          tgATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCaaa                                                                                                                     <  3:516331/149‑1 (MQ=255)
                          tgATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCaa                                                                                                                      <  1:304874/148‑1 (MQ=255)
                          tgATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCaa                                                                                                                      <  2:216250/148‑1 (MQ=255)
                            aTTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAaga                                                                                                                   <  3:255398/149‑1 (MQ=255)
                               ggCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAgaga                                                                                                                 <  1:499573/148‑1 (MQ=255)
                               ggCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAgaga                                                                                                                 <  2:808520/148‑1 (MQ=255)
                                  gATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCGACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGc                                                                                                              <  1:157107/148‑1 (MQ=255)
                                   aTCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAGCGTTACGCTCACGTAGTGCGTAATACTAAAGAGACGCa                                                                                                             >  2:465263/1‑148 (MQ=255)
                                    tCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAg                                                                                                            >  2:75028/1‑148 (MQ=255)
                                      gcgcgACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGACACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt                                                                                                         <  3:562742/149‑1 (MQ=255)
                                      gcgcgACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt                                                                                                         <  4:484284/149‑1 (MQ=255)
                                        gcgACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGa                                                                                                       <  4:142879/149‑1 (MQ=255)
                                         cgaccgacATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAATGa                                                                                                       <  2:274765/148‑1 (MQ=255)
                                           agcgacATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGt                                                                                                    >  4:540949/3‑149 (MQ=255)
                                              gacATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCa                                                                                                 <  3:237479/149‑1 (MQ=255)
                                               acATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCa                                                                                                 <  1:810828/148‑1 (MQ=255)
                                                  ttCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGgt                                                                                              >  1:469287/1‑148 (MQ=255)
                                                    cAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg                                                                                           <  4:444640/149‑1 (MQ=255)
                                                     aaCTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACTTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTgg                                                                                          >  3:773316/1‑149 (MQ=255)
                                                       cTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCa                                                                                                                                        <  3:240949/101‑1 (MQ=255)
                                                       cTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCa                                                                                                                                        >  4:240949/1‑101 (MQ=255)
                                                       cTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc                                                                                         >  2:255468/1‑148 (MQ=255)
                                                           cGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGa                                                                                     >  2:833850/1‑148 (MQ=255)
                                                            ggAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATc                                                                                   <  4:497895/149‑1 (MQ=255)
                                                             gAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCg                                                                                  <  3:628487/149‑1 (MQ=255)
                                                              aaaaCATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCg                                                                                  <  1:231815/148‑1 (MQ=255)
                                                               aaaCATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGt                                                                                 >  2:354383/1‑148 (MQ=255)
                                                                 aCATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGa                                                                               >  1:183821/1‑148 (MQ=255)
                                                                  cATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGaa                                                                              <  1:354383/148‑1 (MQ=255)
                                                                    tGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCGCGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGt                                                                           <  3:173501/149‑1 (MQ=255)
                                                                    tGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGt                                                                           <  4:479396/149‑1 (MQ=255)
                                                                    tGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGt                                                                           >  3:42314/1‑149 (MQ=255)
                                                                      ggCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTg                                                                          <  1:430506/148‑1 (MQ=255)
                                                                        cATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCg                                                                                  <  1:346002/138‑1 (MQ=255)
                                                                        cATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCg                                                                                  >  2:346002/1‑138 (MQ=255)
                                                                          ttACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc                                                                                         >  4:475352/1‑129 (MQ=255)
                                                                          ttACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc                                                                                         <  3:475352/129‑1 (MQ=255)
                                                                            aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGgcagca                                                                    <  1:255468/148‑1 (MQ=255)
                                                                             cTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCaa                                                                   <  2:469287/148‑1 (MQ=255)
                                                                              tGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGa                                                                 >  4:156731/1‑149 (MQ=255)
                                                                                gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGAtt                                                               <  4:42314/149‑1 (MQ=255)
                                                                                gTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGAt                                                                <  1:471367/148‑1 (MQ=255)
                                                                                 tttACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGAtt                                                               >  1:103124/1‑148 (MQ=255)
                                                                                 tttACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGAGTa                                                              >  3:598929/1‑149 (MQ=255)
                                                                                  ttACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTa                                                              <  1:168057/148‑1 (MQ=255)
                                                                                   tACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGt                                                                                                                                  >  4:543483/1‑79 (MQ=255)
                                                                                   tACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGt                                                                                                                                  <  3:543483/79‑1 (MQ=255)
                                                                                   tACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAac                                                            >  4:883903/1‑149 (MQ=255)
                                                                                       cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACAcc                                                         >  1:912648/1‑148 (MQ=255)
                                                                                         aCGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGa                                                                 <  1:804048/138‑1 (MQ=255)
                                                                                         aCGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGa                                                                 >  2:804048/1‑138 (MQ=255)
                                                                                            aCCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGtt                                                   <  3:550191/149‑1 (MQ=255)
                                                                                            aCCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGt                                                    >  1:729364/1‑148 (MQ=255)
                                                                                             ccGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGtt                                                   <  2:183821/148‑1 (MQ=255)
                                                                                             ccGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTCAAGGTGGCAGCAAGATTAACACCGGCGtt                                                   >  1:658564/1‑148 (MQ=255)
                                                                                              cgcgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTg                                                  >  2:701283/1‑148 (MQ=255)
                                                                                                  aaaCCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGcttc                                             <  3:540949/149‑1 (MQ=255)
                                                                                                      ccTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATAGCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGTCTTCTTTg                                         >  3:239585/1‑149 (MQ=255)
                                                                                                       cTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGa                                        >  3:429022/1‑149 (MQ=255)
                                                                                                        tGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGa                                        >  1:70057/1‑148 (MQ=255)
                                                                                                        tGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGAt                                       <  3:905913/149‑1 (MQ=255)
                                                                                                          aaCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGAtt                                                               <  3:97891/123‑1 (MQ=255)
                                                                                                                ccAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGc                                <  2:398644/148‑1 (MQ=255)
                                                                                                                 cAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTg                              <  3:257360/149‑1 (MQ=255)
                                                                                                                  aaTGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCaa                                                                   >  3:261037/1‑111 (MQ=255)
                                                                                                                  aaTGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCaa                                                                   <  4:261037/111‑1 (MQ=255)
                                                                                                                    tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATc                                                                                   >  3:538296/1‑93 (MQ=255)
                                                                                                                    tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATc                                                                                   <  4:538296/93‑1 (MQ=255)
                                                                                                                    tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGAt                           <  3:754529/149‑1 (MQ=255)
                                                                                                                    tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGAt                           <  3:89645/149‑1 (MQ=255)
                                                                                                                    tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGAt                           <  3:298535/149‑1 (MQ=255)
                                                                                                                     gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATc                          >  3:355768/1‑149 (MQ=255)
                                                                                                                        tGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAg                        <  1:126249/148‑1 (MQ=255)
                                                                                                                          gCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTCATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGAt                      >  2:181020/1‑148 (MQ=255)
                                                                                                                              gCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTa                 <  3:156731/149‑1 (MQ=255)
                                                                                                                                aaCTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTAc                <  2:483304/148‑1 (MQ=255)
                                                                                                                                   tCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCAc            >  4:299998/1‑149 (MQ=255)
                                                                                                                                    cACCTGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCAc            >  2:39509/1‑148 (MQ=255)
                                                                                                                                     aCCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGa                                        <  3:844168/119‑1 (MQ=255)
                                                                                                                                     aCCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGa                                        >  4:844168/1‑119 (MQ=255)
                                                                                                                                      cactaCGTGAGCGTAACGGTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGa                       >  2:209485/5‑135 (MQ=255)
                                                                                                                                      cactaCGTGAGCGTAACGGTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGa                       <  1:209485/131‑1 (MQ=255)
                                                                                                                                        aGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCAcggcg        >  1:512185/1‑148 (MQ=255)
                                                                                                                                         taCGTGAGCGTAACGGTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGt      >  4:403165/2‑149 (MQ=255)
                                                                                                                                          aCGTGAGCGTAACGGTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGtt                                                   >  2:628148/1‑103 (MQ=255)
                                                                                                                                          aCGTGAGCGTAACGGTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGtt                                                   <  1:628148/103‑1 (MQ=255)
                                                                                                                                          aCGTGAGCGTAACGGTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTa                 >  2:721227/1‑137 (MQ=255)
                                                                                                                                          aCGTGAGCGTAACGGTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTa                 <  1:721227/137‑1 (MQ=255)
                                                                                                                                          aCGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGtt     <  4:355768/149‑1 (MQ=255)
                                                                                                                                              gACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTcc  >  1:170840/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
AGCGATGGATCGCGCTAACAGTTATGTGATTGGCGATCGCGCGACCGACATTCAACTGGCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAACACCGGCGTTGGCTTCTTTGATCATATGCTGGATCAGATCGCTACCCACGGCGGTTTCC  >  NC_000913/2093821‑2094110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: