Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,103,107 T→G 10.8% pseudogene (283/446 nt) wbbL ← pseudogene, lipopolysaccharide biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,103,1070TG10.8% 234.1 / 18.0 73pseudogene (283/446 nt)wbbLpseudogene, lipopolysaccharide biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base T (32/33);  new base G (3/5);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAATATAGTCCAGGCCTGCATAATGCTG  >  NC_000913/2102962‑2103251
                                                                                                                                                 |                                                                                                                                                
gACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCat                                                                                                                                               <  3:806195/149‑1 (MQ=255)
    caATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCatgat                                                                                                                                            >  1:283683/1‑148 (MQ=255)
    caATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCatgat                                                                                                                                            >  1:828338/1‑148 (MQ=255)
     aaTAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCAtgatga                                                                                                                                          >  4:868610/1‑149 (MQ=255)
      aTAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGAAGCATgatgat                                                                                                                                         <  3:616193/149‑1 (MQ=255)
       tAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATgatgat                                                                                                                                         >  2:122692/1‑148 (MQ=255)
       tAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGata                                                                                                                                        <  3:357940/149‑1 (MQ=255)
        aaaaTCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGAAGCATgatgattt                                                                                                                                       <  4:819149/149‑3 (MQ=255)
          aaTCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCGTGAAGCATgatgatttg                                                                                                                                      <  2:102104/148‑4 (MQ=255)
            tCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCggg                                                                                                                                   >  4:867876/1‑149 (MQ=255)
             cAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCggg                                                                                                                                   <  2:558160/148‑1 (MQ=255)
             cAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGa                                                                                                                                  <  3:86708/149‑1 (MQ=255)
             cAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGa                                                                                                                                  <  3:636047/149‑1 (MQ=255)
              aGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGa                                                                                                                                  <  2:374580/148‑1 (MQ=255)
                     acaGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCa                                                                                                                                                <  3:208622/127‑1 (MQ=255)
                     acaGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCa                                                                                                                                                >  4:208622/1‑127 (MQ=255)
                      caGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCaaaaa                                                                                                                          >  2:157401/1‑148 (MQ=255)
                         gAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACaa                                                                                                                       >  2:790497/1‑148 (MQ=255)
                           aaTTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACaaaa                                                                                                                     <  2:828338/148‑1 (MQ=255)
                             ttttCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATg                                                                                                                   <  2:832257/148‑1 (MQ=255)
                               ttCTTACGGTATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCAGAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATGTCGGGATTCAAAAACAAAATGTaa                                                                                                                <  3:565175/149‑1 (MQ=255)
                               ttCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTa                                                                                                                 <  1:481389/148‑1 (MQ=255)
                                      ggAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAatcatcat                                                                                                         >  3:800218/1‑149 (MQ=255)
                                           aatCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGAAGCAtgatga                                                                                                                                          >  1:823232/1‑111 (MQ=255)
                                           aatCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGAAGCAtgatga                                                                                                                                          <  2:823232/111‑1 (MQ=255)
                                                    aaGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCtata                                                                                            >  2:518613/1‑148 (MQ=255)
                                                      gATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATAttt                                                                                         <  4:895063/149‑1 (MQ=255)
                                                       aTTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATAttt                                                                                         <  1:790497/148‑1 (MQ=255)
                                                        tttCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATAttttt                                                                                       >  4:427408/1‑149 (MQ=255)
                                                         ttCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTc                                                                                      <  3:418509/149‑1 (MQ=255)
                                                          tCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAGACAAAATGTAATCATCATCTGCGGGTCTATATTTTTc                                                                                      <  2:215362/148‑1 (MQ=255)
                                                           cGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCatga                                                                                                                                             >  2:359607/1‑92 (MQ=255)
                                                           cGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCatga                                                                                                                                             <  1:359607/92‑1 (MQ=255)
                                                            gCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGAAGCAtgatga                                                                                                                                          <  4:415628/94‑1 (MQ=255)
                                                             cTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCtt                                                                                   >  2:312364/1‑148 (MQ=255)
                                                              ttCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTa                                                                                 <  3:868610/149‑1 (MQ=255)
                                                               tCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTa                                                                                 <  2:148905/148‑1 (MQ=255)
                                                                  tctcGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATa                                                                             >  3:432179/1‑149 (MQ=255)
                                                                                   tGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAAtatta                                                             <  1:312364/148‑1 (MQ=255)
                                                                                    gTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAAtattatt                                                           <  3:867876/149‑1 (MQ=255)
                                                                                       cTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAAtattattat                                                         <  1:122692/148‑1 (MQ=255)
                                                                                         aaaaGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAAtattattatta                                                       <  2:55008/148‑1 (MQ=255)
                                                                                           aaGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATg                                                     >  1:451167/1‑148 (MQ=255)
                                                                                               aTAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCaa                                                                  <  3:815599/131‑1 (MQ=255)
                                                                                               aTAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCaa                                                                  >  4:815599/1‑131 (MQ=255)
                                                                                                tAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCaaa                                               >  3:5887/1‑149 (MQ=255)
                                                                                                tAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCaaa                                               >  4:361435/1‑149 (MQ=255)
                                                                                                 aaCGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCaaa                                               >  2:169388/1‑148 (MQ=255)
                                                                                                   cGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGc                                             >  2:547866/1‑148 (MQ=255)
                                                                                                      ttACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGt                                          >  2:664340/1‑148 (MQ=255)
                                                                                                       tACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCttt                                                                                  <  1:306653/107‑1 (MQ=255)
                                                                                                       tACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCttt                                                                                  >  2:306653/1‑107 (MQ=255)
                                                                                                          tttCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTAc                                                                                >  3:132864/1‑106 (MQ=255)
                                                                                                          tttCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTAc                                                                                <  4:132864/106‑1 (MQ=255)
                                                                                                          tttCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAAtattattattat                                                      <  4:538220/132‑1 (MQ=255)
                                                                                                          tttCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAAtattattattat                                                      >  3:538220/1‑132 (MQ=255)
                                                                                                              gACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCt                                  <  1:227121/148‑1 (MQ=255)
                                                                                                                cATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCa                               <  3:713848/149‑1 (MQ=255)
                                                                                                                      tAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAat                          >  1:690070/1‑148 (MQ=255)
                                                                                                                              gTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAATATAGTCCa                  <  1:518613/148‑1 (MQ=255)
                                                                                                                              gTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAATATAGTCCAg                 >  3:132062/1‑149 (MQ=255)
                                                                                                                               tCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAATATAGTCCAgg                >  3:861763/1‑149 (MQ=255)
                                                                                                                                cAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAATATAGTCCAGGc               <  4:800218/149‑1 (MQ=255)
                                                                                                                                  gCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAATATAGTCCAGGcc              >  1:89637/1‑148 (MQ=255)
                                                                                                                                   gatatcatcATGAAGCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAATATAGTCCAGGCCTg            >  3:378973/4‑149 (MQ=255)
                                                                                                                                      atcatcATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAATATAGTCCAGGCCTGCa          >  1:210701/1‑148 (MQ=255)
                                                                                                                                      atcatcATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAATATAGTCCAGGCCTGCAt         >  3:397257/1‑149 (MQ=255)
                                                                                                                                       tcatcaTGAAGCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAATATAGTCCAGGCCTGCAt         >  1:670531/1‑148 (MQ=255)
                                                                                                                                         atcatGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAATATAGTCCAg                 <  4:64130/138‑1 (MQ=255)
                                                                                                                                         atcatGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAATATAGTCCAg                 >  3:64130/1‑138 (MQ=255)
                                                                                                                                           catGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAATATAGTCCAGGCCTGCATAATg     >  1:553307/1‑148 (MQ=255)
                                                                                                                                             tGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAATATAGTCCAGGCCTGCATAATGCt   <  2:690070/148‑1 (MQ=255)
                                                                                                                                              gCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAATATAGTCCAGGCCTGCATAATGCTg  <  2:553307/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GACACAATAAAATCAGAAAGCACAGGAAATTTTCTTACGGAATAATCATGTAAAGATTTCGCTTCATCTCGGAACAGGCATAATGTACTAAAAGCATAACGCTTACTTTCGACATATTTAATATATGTCAGCAAATCATCATGCTTCATGATGATATCGGGATTCAAAAACAAAATGTAATCATCATCTGCGGGTCTATATTTTTCCTTTACATACGCCACCGCAATATTATTATTATGACCAAAGCCGTATACACCTCCACTAATATAGTCCAGGCCTGCATAATGCTG  >  NC_000913/2102962‑2103251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: