Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,147,470 A→G 5.7% V24A (GTG→GCG)  alkA ← 3‑methyl‑adenine DNA glycosylase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,147,4700AG 5.7% 225.8 / 3.7 70V24A (GTG→GCG) alkA3‑methyl‑adenine DNA glycosylase II
Reads supporting (aligned to +/- strand):  ref base A (32/34);  new base G (2/2);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.66e-01

CTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGT  >  NC_000913/2147322‑2147614
                                                                                                                                                    |                                                                                                                                                
ctctGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCa                                                                                                                                                  <  4:9774/149‑1 (MQ=255)
 tctGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCAc                                                                                                                                                 >  4:652177/1‑149 (MQ=255)
               ttCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCGCCGCCCGtgcggtg                                                                                                                                    >  2:245038/1‑146 (MQ=255)
               ttCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCGCCGCCCGtgcggtg                                                                                                                                    >  2:599751/1‑146 (MQ=255)
               ttCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGgcggcg                                                                                                                                    <  2:282212/148‑1 (MQ=255)
               ttCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGCCga                                                                                                                                   <  4:262705/149‑1 (MQ=255)
                tCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCGCCGCCCGTGCGGTga                                                                                                                                   <  1:245038/148‑1 (MQ=255)
                tCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCGCCGCCCGTGCGGTga                                                                                                                                   <  1:599751/148‑1 (MQ=255)
                      ccTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATc                                                                                                                             <  2:10418/148‑1 (MQ=255)
                       cTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATccc                                                                                                                           <  3:776333/149‑1 (MQ=255)
                       cTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATcc                                                                                                                            >  2:291329/1‑148 (MQ=255)
                        tGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATccc                                                                                                                           >  1:481171/1‑148 (MQ=255)
                          cACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCaa                                                                                                                         <  1:663413/148‑1 (MQ=255)
                            cTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACa                                                                                                                       <  2:685978/148‑1 (MQ=255)
                              tAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAAt                                                                                                                              >  2:886217/1‑139 (MQ=255)
                              tAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAAt                                                                                                                              <  1:886217/139‑1 (MQ=255)
                              tAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATc                                                                                                                     <  2:876523/148‑1 (MQ=255)
                                  tttATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACg                                                                                                                 >  1:384033/1‑148 (MQ=255)
                                   ttATGTGCAGAGTATGGCGGGCTATATCAGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGa                                                                                                                <  2:699173/148‑1 (MQ=255)
                                           agagTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCa                                                                                                         >  3:287660/1‑147 (MQ=255)
                                           agagTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCa                                                                                                         <  4:287660/147‑1 (MQ=255)
                                           agagTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATa                                                                                                       >  3:750436/1‑149 (MQ=255)
                                             agTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATAcg                                                                                                     <  4:726807/149‑1 (MQ=255)
                                                   gCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACggcggc                                                                                                <  2:761772/148‑1 (MQ=255)
                                                          atatCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACGTCCACGACCAGTCATACGGCGGCTGCCAGtt                                                                                        <  3:38106/149‑1 (MQ=255)
                                                             tCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAg                                                                                     >  3:389082/1‑149 (MQ=255)
                                                             tCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAg                                                                                     >  4:469057/1‑149 (MQ=255)
                                                             tCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAg                                                                                     >  3:507002/1‑149 (MQ=255)
                                                              ccGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCTAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCGCGTTCAg                                                                                     <  2:709908/148‑1 (MQ=255)
                                                              ccGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAg                                                                                     <  2:384033/148‑1 (MQ=255)
                                                              ccGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAg                                                                                     <  1:319967/148‑1 (MQ=255)
                                                              ccGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAg                                                                                     <  2:234829/148‑1 (MQ=255)
                                                               cGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAgg                                                                                    <  1:282522/148‑1 (MQ=255)
                                                                  aaTAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGta                                                                                 >  1:780308/1‑148 (MQ=255)
                                                                      gCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACAt                                                                            <  3:810482/149‑1 (MQ=255)
                                                                       cAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATc                                                                           <  3:638396/149‑1 (MQ=255)
                                                                           caccacGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACAGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCa                                                                        >  1:589859/1‑148 (MQ=255)
                                                                            accacGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCAtc                                                                      >  3:471821/1‑149 (MQ=255)
                                                                            accacGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCAtc                                                                      >  4:680931/1‑149 (MQ=255)
                                                                            accacGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCAtc                                                                      >  4:633799/1‑149 (MQ=255)
                                                                              cacGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCAtctc                                                                    >  3:144784/1‑149 (MQ=255)
                                                                              cacGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCAtct                                                                     >  1:483723/1‑148 (MQ=255)
                                                                               acGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTcc                                                                   >  4:178133/1‑149 (MQ=255)
                                                                                    gcgATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTAtt                                                              >  4:85156/1‑149 (MQ=255)
                                                                                       atatTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCa                                                            >  1:433873/1‑148 (MQ=255)
                                                                                                 aCCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCa                                                  >  1:73049/1‑148 (MQ=255)
                                                                                                   cGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCa                                               <  3:447283/149‑1 (MQ=255)
                                                                                                      cAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCAGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATa                                             <  2:780308/148‑1 (MQ=255)
                                                                                                          cTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACg                                         >  1:240106/1‑148 (MQ=255)
                                                                                                             cGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTa                                      <  1:550473/148‑1 (MQ=255)
                                                                                                             cGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTa                                      <  2:589859/148‑1 (MQ=255)
                                                                                                               ggCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTAtt                                    >  2:242289/1‑148 (MQ=255)
                                                                                                               ggCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTc                                   >  3:321107/1‑149 (MQ=255)
                                                                                                                gCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCa                                  >  3:404294/1‑149 (MQ=255)
                                                                                                                 cATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCa                                  >  1:742083/1‑148 (MQ=255)
                                                                                                                     ataaCTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGc                             >  4:497942/1‑149 (MQ=255)
                                                                                                                          tGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTgcgc                        >  4:447506/1‑149 (MQ=255)
                                                                                                                           gTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTgcgc                        >  1:500073/1‑148 (MQ=255)
                                                                                                                            tCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCgct                       >  2:613743/1‑148 (MQ=255)
                                                                                                                            tCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCgct                       <  2:742083/148‑1 (MQ=255)
                                                                                                                             ccGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCgcttt                     <  4:321107/149‑1 (MQ=255)
                                                                                                                               gcgACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCgctttgc                   <  3:497942/149‑1 (MQ=255)
                                                                                                                               gcgACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCgctttgc                   <  3:469057/149‑1 (MQ=255)
                                                                                                                                  aCCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCgctttgcttt                <  3:178133/149‑1 (MQ=255)
                                                                                                                                  aCCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCgctttgctt                 <  2:282313/148‑1 (MQ=255)
                                                                                                                                   ccGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCgctttgcttt                >  1:797366/1‑148 (MQ=255)
                                                                                                                                      tttCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCat             <  1:613743/148‑1 (MQ=255)
                                                                                                                                         ccACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATAtt          <  1:291329/148‑1 (MQ=255)
                                                                                                                                           acacTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTcc        >  1:693078/1‑148 (MQ=255)
                                                                                                                                              cTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGt     <  1:242289/148‑1 (MQ=255)
                                                                                                                                                 cTCACCGCATGGCAGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGt  <  2:702943/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CTCTGCGGCAACAGGTTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGT  >  NC_000913/2147322‑2147614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑