Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,147,475 A→C 5.5% R22R (CGT→CGG alkA ← 3‑methyl‑adenine DNA glycosylase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,147,4750AC 5.5% 254.9 / 7.4 73R22R (CGT→CGGalkA3‑methyl‑adenine DNA glycosylase II
Reads supporting (aligned to +/- strand):  ref base A (32/37);  new base C (2/2);  total (34/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.82e-01

TTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACGG  >  NC_000913/2147337‑2147622
                                                                                                                                          |                                                                                                                                                   
ttCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCGCCGCCCGtgcggtg                                                                                                                                            >  2:245038/1‑146 (MQ=255)
ttCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCGCCGCCCGtgcggtg                                                                                                                                            >  2:599751/1‑146 (MQ=255)
ttCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGgcggcg                                                                                                                                            <  2:282212/148‑1 (MQ=255)
ttCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGCCga                                                                                                                                           <  4:262705/149‑1 (MQ=255)
 tCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCGCCGCCCGTGCGGTga                                                                                                                                           <  1:599751/148‑1 (MQ=255)
 tCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCGCCGCCCGTGCGGTga                                                                                                                                           <  1:245038/148‑1 (MQ=255)
       ccTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATc                                                                                                                                     <  2:10418/148‑1 (MQ=255)
        cTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATccc                                                                                                                                   <  3:776333/149‑1 (MQ=255)
        cTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATcc                                                                                                                                    >  2:291329/1‑148 (MQ=255)
         tGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATccc                                                                                                                                   >  1:481171/1‑148 (MQ=255)
           cACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCaa                                                                                                                                 <  1:663413/148‑1 (MQ=255)
             cTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACa                                                                                                                               <  2:685978/148‑1 (MQ=255)
               tAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAAt                                                                                                                                      <  1:886217/139‑1 (MQ=255)
               tAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAAt                                                                                                                                      >  2:886217/1‑139 (MQ=255)
               tAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATc                                                                                                                             <  2:876523/148‑1 (MQ=255)
                   tttATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACg                                                                                                                         >  1:384033/1‑148 (MQ=255)
                    ttATGTGCAGAGTATGGCGGGCTATATCAGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGa                                                                                                                        <  2:699173/148‑1 (MQ=255)
                            agagTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCa                                                                                                                 >  3:287660/1‑147 (MQ=255)
                            agagTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCa                                                                                                                 <  4:287660/147‑1 (MQ=255)
                            agagTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATa                                                                                                               >  3:750436/1‑149 (MQ=255)
                              agTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATAcg                                                                                                             <  4:726807/149‑1 (MQ=255)
                                    gCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACggcggc                                                                                                        <  2:761772/148‑1 (MQ=255)
                                           atatCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACGTCCACGACCAGTCATACGGCGGCTGCCAGtt                                                                                                <  3:38106/149‑1 (MQ=255)
                                              tCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAg                                                                                             >  4:469057/1‑149 (MQ=255)
                                              tCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAg                                                                                             >  3:389082/1‑149 (MQ=255)
                                              tCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAg                                                                                             >  3:507002/1‑149 (MQ=255)
                                               ccGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCTAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCGCGTTCAg                                                                                             <  2:709908/148‑1 (MQ=255)
                                               ccGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAg                                                                                             <  2:234829/148‑1 (MQ=255)
                                               ccGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAg                                                                                             <  2:384033/148‑1 (MQ=255)
                                               ccGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAg                                                                                             <  1:319967/148‑1 (MQ=255)
                                                cGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAgg                                                                                            <  1:282522/148‑1 (MQ=255)
                                                   aaTAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGta                                                                                         >  1:780308/1‑148 (MQ=255)
                                                       gCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACAt                                                                                    <  3:810482/149‑1 (MQ=255)
                                                        cAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATc                                                                                   <  3:638396/149‑1 (MQ=255)
                                                            caccacGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACAGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCa                                                                                >  1:589859/1‑148 (MQ=255)
                                                             accacGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCAtc                                                                              >  3:471821/1‑149 (MQ=255)
                                                             accacGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCAtc                                                                              >  4:633799/1‑149 (MQ=255)
                                                             accacGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCAtc                                                                              >  4:680931/1‑149 (MQ=255)
                                                               cacGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCAtctc                                                                            >  3:144784/1‑149 (MQ=255)
                                                               cacGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCAtct                                                                             >  1:483723/1‑148 (MQ=255)
                                                                acGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTcc                                                                           >  4:178133/1‑149 (MQ=255)
                                                                     gcgATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTAtt                                                                      >  4:85156/1‑149 (MQ=255)
                                                                        atatTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCa                                                                    >  1:433873/1‑148 (MQ=255)
                                                                                  aCCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCa                                                          >  1:73049/1‑148 (MQ=255)
                                                                                    cGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCa                                                       <  3:447283/149‑1 (MQ=255)
                                                                                       cAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCAGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATa                                                     <  2:780308/148‑1 (MQ=255)
                                                                                           cTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACg                                                 >  1:240106/1‑148 (MQ=255)
                                                                                              cGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTa                                              <  1:550473/148‑1 (MQ=255)
                                                                                              cGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTa                                              <  2:589859/148‑1 (MQ=255)
                                                                                                ggCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTAtt                                            >  2:242289/1‑148 (MQ=255)
                                                                                                ggCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTc                                           >  3:321107/1‑149 (MQ=255)
                                                                                                 gCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCa                                          >  3:404294/1‑149 (MQ=255)
                                                                                                  cATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCa                                          >  1:742083/1‑148 (MQ=255)
                                                                                                      ataaCTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGc                                     >  4:497942/1‑149 (MQ=255)
                                                                                                           tGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTgcgc                                >  4:447506/1‑149 (MQ=255)
                                                                                                            gTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTgcgc                                >  1:500073/1‑148 (MQ=255)
                                                                                                             tCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCgct                               >  2:613743/1‑148 (MQ=255)
                                                                                                             tCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCgct                               <  2:742083/148‑1 (MQ=255)
                                                                                                              ccGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCgcttt                             <  4:321107/149‑1 (MQ=255)
                                                                                                                gcgACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCgctttgc                           <  3:469057/149‑1 (MQ=255)
                                                                                                                gcgACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCgctttgc                           <  3:497942/149‑1 (MQ=255)
                                                                                                                   aCCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCgctttgcttt                        <  3:178133/149‑1 (MQ=255)
                                                                                                                   aCCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCgctttgctt                         <  2:282313/148‑1 (MQ=255)
                                                                                                                    ccGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCgctttgcttt                        >  1:797366/1‑148 (MQ=255)
                                                                                                                       tttCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCat                     <  1:613743/148‑1 (MQ=255)
                                                                                                                          ccACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATAtt                  <  1:291329/148‑1 (MQ=255)
                                                                                                                            acacTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTcc                >  1:693078/1‑148 (MQ=255)
                                                                                                                               cTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGt             <  1:242289/148‑1 (MQ=255)
                                                                                                                                  cTCACCGCATGGCAGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGt          <  2:702943/148‑1 (MQ=255)
                                                                                                                                      ccGCACGGGCGCCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGa     <  3:785424/149‑1 (MQ=255)
                                                                                                                                         cACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACgg  >  3:380725/1‑149 (MQ=255)
                                                                                                                                         cACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACg   <  2:797366/148‑1 (MQ=255)
                                                                                                                                         cACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACg   <  2:481171/148‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                   
TTCTAAACCTGCACTTAAATTTATGTGCAGAGTATGGCGGGCTATATCCGGAATAGCAGTCACCACGCCGCGATATTCGCCCACCGCCAGACTACGGGCATAATAACTGTCCGCGACCGTTTCCACACTGCTCACCGCACGGGCGGCGAGAAATCCCAACATCCACGACCAGTCATACGGCGGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACGG  >  NC_000913/2147337‑2147622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: