Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,373,177 G→A 9.3% G96S (GGC→AGC)  arnF → undecaprenyl phosphate‑alpha‑L‑ara4N exporter; flippase ArnEF subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,373,1770GA 9.3% 390.5 / 21.6 128G96S (GGC→AGC) arnFundecaprenyl phosphate‑alpha‑L‑ara4N exporter; flippase ArnEF subunit
Reads supporting (aligned to +/- strand):  ref base G (60/55);  new base A (8/4);  total (69/59)
Fisher's exact test for biased strand distribution p-value = 3.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 130 total aligned reads displayed.

CCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCTATA  >  NC_000913/2373031‑2373325
                                                                                                                                                   |                                                                                                                                                    
cccGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCgg                                                                                                                                                     <  1:758026/148‑1 (MQ=255)
    gATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTggg                                                                                                                                                >  4:234423/1‑149 (MQ=255)
      tACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGa                                                                                                                                               <  1:662479/148‑1 (MQ=255)
      tACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGa                                                                                                                                               <  1:15909/148‑1 (MQ=255)
       aCTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGaa                                                                                                                                              <  1:723240/148‑1 (MQ=255)
         tGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAgg                                                                                                                                            <  1:567901/148‑1 (MQ=255)
         tGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTAGGAAggg                                                                                                                                           >  3:778717/1‑149 (MQ=255)
         tGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCAGCCGGGAAgta                                                                                                                                           <  3:558087/149‑3 (MQ=255)
          gTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGa                                                                                                                                          <  3:243990/149‑1 (MQ=255)
          gTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGa                                                                                                                                          <  3:840732/149‑1 (MQ=255)
          gTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGa                                                                                                                                          >  4:598526/1‑149 (MQ=255)
          gTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCAGCCGGGAAgta                                                                                                                                           <  1:45301/148‑3 (MQ=255)
           ttACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAggg                                                                                                                                           >  2:651953/1‑147 (MQ=255)
           atACTCGGGCTGCTGGGTTATCTGCTTTTCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGAc                                                                                                                                         <  4:778717/148‑1 (MQ=255)
            tACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCTTAAATTGCGGCAAGCAAAAGCCTTTCCTTTCTCAGTATGGGGTTTGTGGTGGTATGGAATTCTTCCATTGTACTTCCCGGCATGGAAggaatc                                                                                                                                        >  1:264634/1‑144 (MQ=255)
                      gctgGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTg                                                                                                                               >  1:213528/1‑148 (MQ=255)
                        tgGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGaaa                                                                                                                            <  4:179258/149‑1 (MQ=255)
                         gtgTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATTAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTAGGGATTGCGTCAATGTTACTTCCCGGCTGGGAAGAGACTTTTTCGCTGaaa                                                                                                                            <  2:322698/146‑1 (MQ=255)
                              aTCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCacta                                                                                                                      >  4:600472/1‑149 (MQ=255)
                               tCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCactac                                                                                                                     >  3:520561/1‑149 (MQ=255)
                                  gCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTAGGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTg                                                                                                                   >  2:17992/1‑148 (MQ=255)
                                    tttCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTggg                                                                                                                 <  2:139554/148‑1 (MQ=255)
                                    tttCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGa                                                                                                                >  3:224987/1‑149 (MQ=255)
                                     ttCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGaa                                                                                                                             <  2:564116/135‑1 (MQ=255)
                                      tCCGTTTTTTGTTGGTATAAAACGCTTCATAATCTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCAATACTGGGAGt                                                                                                              >  4:775254/1‑149 (MQ=255)
                                           tttttGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCt                                                                                                         <  3:837319/149‑1 (MQ=255)
                                             tttGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGt                                                                                                       <  3:600472/149‑1 (MQ=255)
                                              ttgttgGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGta                                                                                                      <  3:386103/149‑1 (MQ=255)
                                                 ttgGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGtatta                                                                                                   >  4:330879/1‑149 (MQ=255)
                                                 ttgGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGtatta                                                                                                   <  3:330879/149‑1 (MQ=255)
                                                  tgGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGtatta                                                                                                   <  2:274122/148‑1 (MQ=255)
                                                    gTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGa                                                                                                >  3:594551/1‑149 (MQ=255)
                                                     tataAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTCTGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAg                                                                                               >  3:233264/1‑149 (MQ=255)
                                                     tataAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTg                                                                                                                   <  1:363503/129‑1 (MQ=255)
                                                     tataAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGa                                                                                                >  2:860403/1‑148 (MQ=255)
                                                       taAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTTGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCg                                                                                             >  3:811631/1‑149 (MQ=255)
                                                       taAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCg                                                                                             >  4:188278/1‑149 (MQ=255)
                                                         aaaCGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTACTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCggg                                                                                           >  4:825069/1‑149 (MQ=255)
                                                           aCGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGtt                                                                                         <  3:188278/149‑1 (MQ=255)
                                                             gCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTc                                                                                                                                   <  4:750354/105‑1 (MQ=255)
                                                              cTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGAt                                                                                      <  4:84335/149‑1 (MQ=255)
                                                              cTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAAGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGa                                                                                       <  1:427587/148‑1 (MQ=255)
                                                                  aTAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACtttt                                                                                                                                     <  3:500406/98‑1 (MQ=255)
                                                                  aTAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACtttt                                                                                                                                     >  4:500406/1‑98 (MQ=255)
                                                                  aTAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTg                                                                                  <  4:346851/149‑1 (MQ=255)
                                                                   tAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCt                                                                                   <  2:633571/147‑1 (MQ=255)
                                                                   tAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCt                                                                                   >  1:633571/1‑147 (MQ=255)
                                                                       cTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGAtttt                                                                             <  4:224987/149‑1 (MQ=255)
                                                                         tGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTc                                                                            <  2:907011/148‑1 (MQ=255)
                                                                            gcTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGc                                                                        >  4:354240/1‑149 (MQ=255)
                                                                            gcTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGc                                                                        >  3:22562/1‑149 (MQ=255)
                                                                             cTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGc                                                                        <  2:445560/148‑1 (MQ=255)
                                                                               aaGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGccc                                                                      >  2:457734/1‑148 (MQ=255)
                                                                                  cAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACg                                                                   >  1:507667/1‑148 (MQ=255)
                                                                                     aaGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACa                                                                >  4:218383/1‑149 (MQ=255)
                                                                                        ccTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACaaaa                                                             <  4:80832/149‑1 (MQ=255)
                                                                                           aTGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAaaca                                                           <  1:737344/148‑1 (MQ=255)
                                                                                               cTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGaa                                                                                                                             <  2:105603/78‑1 (MQ=255)
                                                                                               cTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGaa                                                                                                                             >  1:105603/1‑78 (MQ=255)
                                                                                                ttGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGtt                                                      <  1:559083/148‑1 (MQ=255)
                                                                                                  gCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACt                                                   >  3:282885/1‑149 (MQ=255)
                                                                                                  gCTCAGTATGAGTTATATGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACt                                                   <  3:771520/149‑1 (MQ=255)
                                                                                                    tCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGaaa                                                                                                                            >  3:21051/1‑74 (MQ=255)
                                                                                                    tCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGaaa                                                                                                                            <  4:21051/74‑1 (MQ=255)
                                                                                                       gTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGtt                                              <  3:154392/149‑1 (MQ=255)
                                                                                                        tATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGtt                                              <  2:741090/148‑1 (MQ=255)
                                                                                                         aTGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGttt                                             >  1:515169/1‑148 (MQ=255)
                                                                                                                        gTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTaca                             >  3:61721/1‑149 (MQ=255)
                                                                                                                        gTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTaca                             >  4:702788/1‑149 (MQ=255)
                                                                                                                             gATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGcc                        <  3:702788/149‑1 (MQ=255)
                                                                                                                             gATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGcc                        >  3:229269/1‑149 (MQ=255)
                                                                                                                             gATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGcc                        >  3:9555/1‑149 (MQ=255)
                                                                                                                              aTTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGcc                        >  1:261431/1‑148 (MQ=255)
                                                                                                                              aTTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCg                       >  4:262829/1‑149 (MQ=255)
                                                                                                                              aTTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCg                       >  3:137607/1‑149 (MQ=255)
                                                                                                                                tGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGt                      >  2:478501/1‑148 (MQ=255)
                                                                                                                                  cTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTcc                   <  4:22562/149‑1 (MQ=255)
                                                                                                                                  cTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTcc                   >  4:417673/1‑149 (MQ=255)
                                                                                                                                   ttCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTccc                  >  3:223737/1‑149 (MQ=255)
                                                                                                                                   ttCAATGGTACTTCCCGGCTGGGAAGGGACTTTCTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACGTTACACTGCCGTTccc                  >  3:572473/1‑149 (MQ=255)
                                                                                                                                     cAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCa                 >  1:112596/1‑148 (MQ=255)
                                                                                                                                     cAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCAc                <  3:262829/149‑1 (MQ=255)
                                                                                                                                       aTGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACt               >  1:271447/1‑148 (MQ=255)
                                                                                                                                         ggTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCa             <  2:93789/148‑1 (MQ=255)
                                                                                                                                          gTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCAt            <  1:890696/148‑1 (MQ=255)
                                                                                                                                           cccTTCCCAGCCGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATc           >  2:719086/3‑148 (MQ=255)
                                                                                                                                            ccTTCCCAGCCGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCgg         >  3:803824/2‑149 (MQ=255)
                                                                                                                                            ccTTCCCAGCCGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCg          >  2:906854/2‑148 (MQ=255)
                                                                                                                                            ccTTCCCAGCCGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCg          >  1:607435/2‑148 (MQ=255)
                                                                                                                                             cTTCCCAGCCGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGtt                     >  1:335315/1‑136 (MQ=255)
                                                                                                                                             cTTCCCAGCCGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGtt                     <  2:335315/136‑1 (MQ=255)
                                                                                                                                             cTTCCCAGCCGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTc                    <  3:183290/137‑1 (MQ=255)
                                                                                                                                             cTTCCCAGCCGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTc                    >  4:183290/1‑137 (MQ=255)
                                                                                                                                             cTTCCCAGCCGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCgg         >  1:844130/1‑148 (MQ=255)
                                                                                                                                              ttCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTGGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAATGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCgggg       >  4:555820/1‑149 (MQ=255)
                                                                                                                                              ttCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGa                                                                  >  4:835269/1‑90 (MQ=255)
                                                                                                                                              ttCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCGGCCCACGa                                                                  <  3:835269/90‑1 (MQ=255)
                                                                                                                                               tCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCgggg       >  2:398488/1‑148 (MQ=255)
                                                                                                                                               tCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTCCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCgggg       >  1:751767/1‑148 (MQ=255)
                                                                                                                                                cccGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGc      >  2:891229/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCTATA  >  NC_000913/2373031‑2373325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: