| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,419,395 | C→G | 7.6% | V132L (GTG→CTG) | yfcE ← | phosphodiesterase activity on bis‑pNPP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,419,395 | 0 | C | G | 7.6% | 318.8 / 14.3 | 92 | V132L (GTG→CTG) | yfcE | phosphodiesterase activity on bis‑pNPP |
| Reads supporting (aligned to +/- strand): ref base C (42/43); new base G (5/2); total (47/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
GCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAG > NC_000913/2419247‑2419542 | gcgACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCAc < 4:722114/149‑1 (MQ=255) cgACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGCATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCAc < 1:588140/148‑1 (MQ=255) gACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACt < 2:10086/148‑1 (MQ=255) gACCTGATCAATGCTACTATCGTCATTGAGTGCGATTACACTGGGTACGTCATTATTCAGCATGCCATACCTCGCCTGACTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTTGAAAAGATCCCCCCGCTGTTCTGCCCCt < 2:743937/148‑1 (MQ=255) tGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCa > 1:246079/1‑148 (MQ=255) cgcAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGAt < 4:580401/149‑1 (MQ=255) gcAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGAt < 2:355516/148‑1 (MQ=255) cAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATgg < 3:416556/149‑1 (MQ=255) cAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATg < 2:631405/148‑1 (MQ=255) aTGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGt < 4:798300/149‑1 (MQ=255) tGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGt < 1:40272/148‑1 (MQ=255) aTACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTAt < 1:572805/148‑1 (MQ=255) tttGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCg > 3:78540/1‑149 (MQ=255) ttGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCg > 1:406409/1‑148 (MQ=255) aTCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTac > 2:25123/1‑148 (MQ=255) tCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTacac > 4:745628/1‑149 (MQ=255) cATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTg > 1:104677/1‑126 (MQ=255) cATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTg < 2:104677/126‑1 (MQ=255) tGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAg > 2:198153/1‑148 (MQ=255) gAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGc < 2:261608/148‑1 (MQ=255) tGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACAcc < 4:503586/142‑1 (MQ=255) tGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACAcc > 3:503586/1‑142 (MQ=255) tGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACAt > 4:426324/1‑149 (MQ=255) tGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACAt > 3:57926/1‑149 (MQ=255) ttACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGtt > 1:244171/1‑148 (MQ=255) tACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTc < 2:406409/148‑1 (MQ=255) acacTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCt < 1:275511/148‑1 (MQ=255) aaGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGttt > 2:727433/1‑148 (MQ=255) aaGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGt > 1:98309/1‑146 (MQ=255) aaGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGt < 2:98309/146‑1 (MQ=255) aaGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTa < 3:745628/149‑1 (MQ=255) gTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTaca > 3:450736/1‑126 (MQ=255) gTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTaca < 4:450736/126‑1 (MQ=255) tACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAg > 3:402852/1‑149 (MQ=255) cGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAgca > 3:878672/1‑149 (MQ=255) cGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAgc < 2:648589/148‑1 (MQ=255) tCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAgcag > 2:577315/1‑148 (MQ=255) tCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAgcag > 1:348517/1‑148 (MQ=255) aTCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGAt > 3:789557/1‑149 (MQ=255) tCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGAt < 1:697738/148‑1 (MQ=255) ccAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGAttt < 3:100604/149‑1 (MQ=255) aGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGAtttt < 1:198153/148‑1 (MQ=255) cATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTaa < 2:220411/127‑1 (MQ=255) cATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTaa > 1:220411/1‑127 (MQ=255) cATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCa > 4:156908/1‑149 (MQ=255) aTAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCa > 2:803057/1‑148 (MQ=255) aTAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCa > 2:559368/1‑148 (MQ=255) aCTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCaaaa < 1:11390/148‑1 (MQ=255) aCTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCaaaa < 2:379540/148‑1 (MQ=255) ccGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATg > 1:800157/1‑148 (MQ=255) ccGGATTAACGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACAACAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGa < 4:422640/149‑1 (MQ=255) gCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCa > 1:229486/1‑142 (MQ=255) gCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCa < 2:229486/142‑1 (MQ=255) ttCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCaaaa > 2:429209/1‑148 (MQ=255) cGGAATACTCACCGAGCCGGGGTTGAAGTGGAAATTCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACAGCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAAc < 1:559368/148‑1 (MQ=255) cGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACa < 4:900876/149‑1 (MQ=255) cGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACa < 3:426324/149‑1 (MQ=255) ggAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACa > 1:37236/1‑148 (MQ=255) aCTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACgt < 2:246079/148‑1 (MQ=255) tCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACgttgt < 3:156908/149‑1 (MQ=255) ccGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGtttt > 3:883940/1‑149 (MQ=255) gAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGttttt > 1:319364/1‑148 (MQ=255) gCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTcc > 1:39596/1‑148 (MQ=255) ccGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCa < 1:577315/148‑1 (MQ=255) gTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAAt < 1:803057/148‑1 (MQ=255) tGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATAcc < 4:883940/149‑1 (MQ=255) aGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTg < 1:518628/148‑1 (MQ=255) aGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTATTCCAGTAATACCTg > 2:264299/1‑148 (MQ=255) ggAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTg > 1:383223/1‑148 (MQ=255) ggAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTg > 2:46199/1‑148 (MQ=255) gAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGcc > 3:566659/1‑149 (MQ=255) aaaaTCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTc > 1:798617/1‑131 (MQ=255) aaaaTCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTc < 2:798617/131‑1 (MQ=255) tctcCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACgg < 2:37236/148‑1 (MQ=255) ccccccGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATa > 1:746780/1‑132 (MQ=255) ccccccGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATa < 2:746780/132‑1 (MQ=255) ccccccGCTGCTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGgcgc > 1:288014/1‑148 (MQ=255) ccccGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAg > 1:701495/1‑110 (MQ=255) ccccGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAg < 2:701495/110‑1 (MQ=255) cccGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGt < 1:155387/134‑1 (MQ=255) cccGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGt > 2:155387/1‑134 (MQ=255) ccGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTg < 1:694871/132‑1 (MQ=255) ccGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTg > 2:694871/1‑132 (MQ=255) gCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTAt < 1:25123/148‑1 (MQ=255) cccatCTGCCAGTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATc > 2:854621/5‑148 (MQ=255) cTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATc > 1:259897/1‑148 (MQ=255) ccatCTGCCAGTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGtt < 4:178081/127‑1 (MQ=255) ccatCTGCCAGTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGtt > 3:178081/4‑130 (MQ=255) ccatCTGCCAGTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCgg > 3:772046/4‑149 (MQ=255) ccatCTGCCAGTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCgg > 4:811320/4‑149 (MQ=255) catCTGCCAGTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCggg < 3:811320/147‑1 (MQ=255) catCTGCCAGTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCggg > 4:856816/3‑149 (MQ=255) cACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATgc > 2:105348/1‑148 (MQ=255) aCTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATgcag < 4:566659/149‑1 (MQ=255) | GCGACCTGCGCAATGATACTTTGATCATTGAGTGCGATTACACTAAGTACGTCATTATCCAGCATGCCATAACTCGCCGGATTACCGCCTTTCGGAATACTCACCGAGCCGGGGTTGAAGTGGAAAATCTCCCCCCGCTGTTCTGCCACTGGCAGATGGGTATGACCGTACACCAGCACATCGTTCTGGTTTAAAGCAGGCAGATTTTCCGGGCCAAAAAGATGACCATGCGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAG > NC_000913/2419247‑2419542 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |