| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,521,348 | A→G | 22.7% | intergenic (+20/+245) | lysV → / ← xapR | tRNA‑Lys/transcriptional activator of xapAB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,521,348 | 0 | A | G | 22.7% | 42.2 / 8.5 | 22 | intergenic (+20/+245) | lysV/xapR | tRNA‑Lys/transcriptional activator of xapAB |
| Reads supporting (aligned to +/- strand): ref base A (9/8); new base G (3/2); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01 | |||||||||||
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAG > NC_000913/2521210‑2521495 | aggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtttt < 2:856939/148‑1 (MQ=255) gggTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTa > 2:868527/1‑148 (MQ=255) gcggTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtttt < 1:534038/136‑1 (MQ=255) gcggTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtttt > 2:534038/1‑136 (MQ=255) cGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGAt > 2:796910/1‑148 (MQ=255) tcaCCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAAc > 3:473469/1‑149 (MQ=255) ccaccaCTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCTTTAGGGCGCttt > 4:693187/1‑114 (MQ=255) ccaccaCTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCTTTAGGGCGCttt < 3:693187/114‑1 (MQ=255) tCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgag < 1:868527/148‑1 (MQ=32) tCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCa < 4:473469/149‑1 (MQ=32) gTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGaaa < 1:796910/148‑1 (MQ=32) aTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACg > 2:384188/1‑148 (MQ=32) aaTCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAg > 3:525224/1‑104 (MQ=32) aaTCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAg < 4:525224/104‑1 (MQ=33) gCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAGACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagcga > 1:698655/1‑148 (MQ=17) ccaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGc < 1:384188/148‑1 (MQ=32) aaaaGCGCCTTTAGGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCATGTttgag < 3:481016/69‑1 (MQ=11) aaaaGCGCCTTTAGGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgag > 4:481016/1‑69 (MQ=11) aGCGCCTTTAGGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttg > 2:366055/1‑64 (MQ=11) ccTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTaaa > 4:654583/1‑149 (MQ=32) tAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAaacaa < 3:372579/77‑1 (MQ=35) tAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAaacaa > 4:372579/1‑77 (MQ=35) aaGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAg < 3:654583/149‑1 (MQ=32) | AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAG > NC_000913/2521210‑2521495 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |