Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,660,611 G→T 5.1% Y307* (TAC→TAA) ‡ iscS ← cysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,660,6110GT 5.1% 426.5 / 9.3 118Y307* (TAC→TAA) ‡iscScysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (55/57);  new base T (2/4);  total (57/61)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

Only 100 of 119 total aligned reads displayed.

AGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTT  >  NC_000913/2660464‑2660755
                                                                                                                                                   |                                                                                                                                                
  agaAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTa                                                                                                                                                >  2:895431/1‑148 (MQ=255)
  agaAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAg                                                                                                                                               <  3:201636/149‑1 (MQ=255)
    aaaCGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGtt                                                                                                                                             >  4:489531/1‑149 (MQ=255)
    aaaCGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGt                                                                                                                                              <  1:51381/148‑1 (MQ=255)
      aCGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTg                                                                                                                                            >  1:753759/1‑148 (MQ=255)
        ggATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGaa                                                                                                                                          <  2:722050/148‑1 (MQ=255)
        ggATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAg                                                                                                                                         <  3:76910/149‑1 (MQ=255)
          aTAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACTACGTTGAAggt                                                                                                                                       <  3:480344/149‑3 (MQ=255)
            agagCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCCCCTTCAACGTAGTTGAAGCTg                                                                                                                                      <  1:118646/148‑1 (MQ=255)
            agagCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGa                                                                                                                                     <  3:89197/149‑1 (MQ=255)
              agCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGAc                                                                                                                                    >  2:806666/1‑148 (MQ=255)
               gCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAg                                                                                                                                         <  1:131199/142‑1 (MQ=255)
               gCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAg                                                                                                                                         >  2:131199/1‑142 (MQ=255)
                 tATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGtt                                                                                                                                 <  2:753759/148‑1 (MQ=255)
                     tgCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTgaga                                                                                                                             <  1:895431/148‑1 (MQ=255)
                      gCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAAt                                                                                                                           <  4:843380/149‑1 (MQ=255)
                       ccAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATg                                                                                                                          >  3:27770/1‑149 (MQ=255)
                        cAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATg                                                                                                                          >  2:353321/1‑148 (MQ=255)
                         aGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACTACGTTGAAg                                                                                                                                         >  3:597092/1‑132 (MQ=255)
                             cgtcgtTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCgg                                                                                                                     >  2:402792/1‑148 (MQ=255)
                             cgtcgtTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGgc                                                                                                                    >  4:336270/1‑149 (MQ=255)
                             cgtcgtTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGgc                                                                                                                    <  4:27770/149‑1 (MQ=255)
                              gtcgtTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGgcg                                                                                                                   >  3:657903/1‑149 (MQ=255)
                               tcgtTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGgcg                                                                                                                   >  2:234720/1‑148 (MQ=255)
                                cgtTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCa                                                                                                                 <  3:543763/149‑1 (MQ=255)
                                    cAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCg                                                                                                              >  1:125839/1‑148 (MQ=255)
                                       ccccAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGtt                                                                                                          >  3:322881/1‑149 (MQ=255)
                                         ccAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGtt                                                                                                                                 >  3:808004/1‑124 (MQ=255)
                                         ccAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGtt                                                                                                                                 <  4:808004/124‑1 (MQ=255)
                                         ccAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTcc                                                                                                        >  4:349617/1‑149 (MQ=255)
                                           aGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCa                                                                                                       <  2:620033/148‑1 (MQ=255)
                                           aGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAg                                                                                                      <  4:387240/149‑1 (MQ=255)
                                               cgcgcAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCa                                                                                                  <  3:433399/149‑1 (MQ=255)
                                                   cagcaCGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCg                                                                                               >  1:249852/1‑148 (MQ=255)
                                                     gcaCGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGtt                                                                                             <  1:717279/148‑1 (MQ=255)
                                                      caCGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTc                                                                                            <  2:733177/148‑1 (MQ=255)
                                                        cGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAgg                                                                                         <  3:336270/149‑1 (MQ=255)
                                                             gACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAAc                                                                                    <  3:349617/149‑1 (MQ=255)
                                                                  ttCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCTAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAActtct                                                                                >  1:74141/1‑148 (MQ=255)
                                                                   tCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCAGCTTCAACTACGTTGAAGGTg                                                                                                                                      <  1:13368/93‑1 (MQ=38)
                                                                   tCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAActtctt                                                                               <  1:164478/148‑1 (MQ=255)
                                                                   tCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAActtctt                                                                               >  2:226238/1‑148 (MQ=255)
                                                                     gAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACTACGTTGAAgg                                                                                                                                        <  2:125463/89‑2 (MQ=255)
                                                                          ctGCTGGCGTACCGGGGGGACCCTAAAGAACTGCGAGGGCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCt                                                                       <  3:245723/148‑1 (MQ=255)
                                                                            gCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt                                                                     <  4:498736/149‑1 (MQ=255)
                                                                            gCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCttt                                                                     <  4:56669/149‑1 (MQ=255)
                                                                            gCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCtt                                                                      <  1:442712/148‑1 (MQ=255)
                                                                              tGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTg                                                                    <  2:912052/148‑1 (MQ=255)
                                                                              tGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGa                                                                   <  4:728754/149‑1 (MQ=255)
                                                                              tGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGa                                                                   <  4:216759/149‑1 (MQ=255)
                                                                              tGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGa                                                                   >  4:65137/1‑149 (MQ=255)
                                                                               gACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGAt                                                                  <  3:65137/149‑1 (MQ=255)
                                                                                        gCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAg                                                                                                                                         >  3:47394/1‑69 (MQ=255)
                                                                                             aCCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGa                                                     <  2:164318/148‑1 (MQ=255)
                                                                                             aCCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGa                                                     <  1:252530/148‑1 (MQ=255)
                                                                                             aCCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGAc                                                    >  3:126114/1‑149 (MQ=255)
                                                                                               cTGAAGAACCTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCcaca                                                       >  3:834871/1‑144 (MQ=255)
                                                                                               cTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCcaca                                                       <  4:834871/144‑1 (MQ=255)
                                                                                                tGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGcc                                                               <  3:806103/135‑1 (MQ=255)
                                                                                                tGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGcc                                                               >  4:806103/1‑135 (MQ=255)
                                                                                                 gaagaaACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGtt                                                >  4:26937/1‑149 (MQ=255)
                                                                                                  aagaaACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGtt                                                >  2:372544/1‑148 (MQ=255)
                                                                                                   agaaACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTa                                               >  1:54512/1‑148 (MQ=255)
                                                                                                    gaaACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCAc                                                                                                                >  3:198387/1‑82 (MQ=255)
                                                                                                    gaaACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCAc                                                                                                                <  4:198387/82‑1 (MQ=255)
                                                                                                     aaaCTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACg                                             >  2:805793/1‑148 (MQ=255)
                                                                                                       aCTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCa                                           <  1:665201/148‑1 (MQ=255)
                                                                                                       aCTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAg                                          <  3:788506/149‑1 (MQ=255)
                                                                                                         tGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGc                                        >  3:503462/1‑149 (MQ=255)
                                                                                                         tGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGc                                        >  3:179603/1‑149 (MQ=255)
                                                                                                           cGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGcc                                       >  2:859701/1‑148 (MQ=255)
                                                                                                           cGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGcc                                       >  2:192235/1‑148 (MQ=255)
                                                                                                               gTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCa                                  >  3:597693/1‑149 (MQ=255)
                                                                                                                tCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCa                                  >  1:195469/1‑148 (MQ=255)
                                                                                                                 cTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAg                                 <  1:226238/148‑1 (MQ=255)
                                                                                                                  tttCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGAc                               >  4:724522/1‑149 (MQ=255)
                                                                                                                    tCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGt                             <  4:8856/149‑1 (MQ=255)
                                                                                                                     cAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGtt                            <  3:489531/149‑1 (MQ=255)
                                                                                                                      aGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGTCGGTTACGCAGGCCGCGCAGACGTTc                           <  3:656478/149‑1 (MQ=255)
                                                                                                                      aGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTc                           <  4:179603/149‑1 (MQ=255)
                                                                                                                         gcCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGa                                                     <  4:201673/120‑1 (MQ=255)
                                                                                                                         gcCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGa                                                     >  3:201673/1‑120 (MQ=255)
                                                                                                                           cATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCtt                                                                      <  4:684148/101‑1 (MQ=255)
                                                                                                                             tAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCAtctc                     <  2:558967/148‑1 (MQ=255)
                                                                                                                                tCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGt                  <  2:125839/148‑1 (MQ=255)
                                                                                                                                  aGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCGGACGTTCCATCTCGGt                  >  1:192251/1‑146 (MQ=255)
                                                                                                                                  aGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCGGACGTTCCATCTCGGt                  <  2:192251/146‑1 (MQ=255)
                                                                                                                                   gCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGcc              >  3:232440/1‑149 (MQ=255)
                                                                                                                                        tCAGCTTCAACTACGTTGAAGGTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCAtctc         >  3:481465/1‑149 (MQ=255)
                                                                                                                                         cACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCAtctc         >  1:637387/1‑148 (MQ=255)
                                                                                                                                         cACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATctct        >  4:690880/1‑149 (MQ=255)
                                                                                                                                         cACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATctct        >  4:715699/1‑149 (MQ=255)
                                                                                                                                           ccTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGc               <  1:163871/140‑1 (MQ=255)
                                                                                                                                           ccTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCcctt       >  1:197749/1‑148 (MQ=255)
                                                                                                                                             ttCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCtcttct     >  1:828704/1‑148 (MQ=255)
                                                                                                                                              tCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAg                                 <  1:672172/119‑1 (MQ=255)
                                                                                                                                              tCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAg                                 >  2:672172/1‑119 (MQ=255)
                                                                                                                                               cAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCtttt  <  4:597693/149‑1 (MQ=255)
                                                                                                                                               cAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCtttt  <  4:322881/149‑1 (MQ=255)
                                                                                                                                               cAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCttt   <  1:188677/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
AGAGAAACGGATAGAGCTATGTGCCAGCTCGTCGTTCAGCCCCAGCGCGCGCAGCACGTAGGACGGTTCGAGGCTTGCTGACGTACAGGCGGAACCTGAAGAAACTGCGAGGTCTTTCAGCGCCATAATCAGCGACTCACCTTCAACGTAGTTGAAGCTGACGTTGAGAATGTTCGGCGCACCGTGTTCCAGGTCACCGTTCAGGTAAACTTCTTCGATATCTTTGATGCCGTTCCACAGACGGTTACGCAGGCCGCGCAGACGTTCCATCTCGGTCGCCATCTCTTCTTTT  >  NC_000913/2660464‑2660755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: