| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,706,606 | G→T | 10.2% | L173L (CTC→CTA) ‡ | lepA ← | back‑translocating elongation factor EF4, GTPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,706,606 | 0 | G | T | 10.2% | 295.9 / 20.2 | 88 | L173L (CTC→CTA) ‡ | lepA | back‑translocating elongation factor EF4, GTPase |
| Reads supporting (aligned to +/- strand): ref base G (46/33); new base T (4/5); total (50/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01 | |||||||||||
TTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGA > NC_000913/2706459‑2706754 | tttGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCga < 3:666070/149‑1 (MQ=255)tttGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCga < 3:486402/149‑1 (MQ=255) gCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCTCGAACGt > 4:336829/1‑149 (MQ=255) cccTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGt > 1:825086/1‑148 (MQ=255) ccTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTcc < 3:700256/149‑1 (MQ=255) cTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCTCGAACGTctg < 4:625281/149‑3 (MQ=255) cTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCTCGAACGTctg < 3:590175/149‑3 (MQ=255) ttACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGTTAGTTGTCGAACTATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCTGAATGTCGCGCACCAGACGTTCTCGAACGtatg < 2:599934/148‑4 (MQ=255) ttACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCTCGAACGTctgg < 3:405017/149‑4 (MQ=255) ttACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCTCGAACGTctgg < 3:336829/149‑4 (MQ=255) tACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTg > 1:590205/1‑148 (MQ=255) cGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGcc > 2:602762/1‑148 (MQ=255) cGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGcc > 1:781249/1‑148 (MQ=255) gTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCg > 2:797569/1‑148 (MQ=255) tttttAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCgg < 2:825086/148‑1 (MQ=255) ttttAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGt > 2:729207/1‑148 (MQ=255) ttAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGtttt < 4:802978/149‑1 (MQ=255) tAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGtttt > 1:171737/1‑148 (MQ=255) aCGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCt > 1:719081/1‑148 (MQ=255) ggATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGa < 1:192624/148‑1 (MQ=255) aTAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACa > 4:415534/1‑149 (MQ=255) gTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAgcgc > 3:904975/1‑149 (MQ=255) tGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCa > 4:706610/1‑149 (MQ=255) aaaCAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCAc > 1:531632/1‑148 (MQ=255) aacGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGt > 4:163440/1‑149 (MQ=255) gCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCgg > 3:609231/1‑149 (MQ=255) cAGGTAGTTGTCGAACCATGAGTCGATAATTAGTTCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGtt > 1:226635/1‑121 (MQ=255) cAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGtt < 2:226635/121‑1 (MQ=255) aGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGc < 3:427485/149‑1 (MQ=255) ggTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACCTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGc < 2:781249/148‑1 (MQ=255) gTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTc < 4:371301/96‑1 (MQ=255) gTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTc > 3:371301/1‑96 (MQ=255) ttGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGa < 2:171737/148‑1 (MQ=255) tGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGAt > 2:81467/1‑148 (MQ=255) cGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGtt < 1:492301/110‑1 (MQ=255) cGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGtt > 2:492301/1‑110 (MQ=255) aaCCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCcgac < 3:163440/149‑1 (MQ=255) ccATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCcgacg > 2:589702/1‑148 (MQ=255) ccATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCcgacg < 2:380382/148‑1 (MQ=255) gAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGatat < 1:164935/148‑1 (MQ=255) gAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATc > 3:351182/1‑149 (MQ=255) tCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTc > 3:74120/1‑149 (MQ=255) cGAGAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTc > 2:821822/1‑148 (MQ=255) gATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCaa > 3:893387/1‑149 (MQ=255) tAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAAtt > 4:842145/1‑149 (MQ=255) aGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATttcttc < 3:892618/149‑1 (MQ=255) gTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATttcttc > 1:750483/1‑148 (MQ=255) ccTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGc < 1:602762/148‑1 (MQ=255) tGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCAc < 3:589736/149‑1 (MQ=255) gCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGATGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCAcg > 4:738785/1‑149 (MQ=255) cGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGtt < 4:74120/149‑1 (MQ=255) gggCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTc > 3:605187/1‑149 (MQ=255) ggCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTc < 1:81467/148‑1 (MQ=255) gCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTACGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCa < 1:267836/148‑1 (MQ=255) tCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCATCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGAt < 1:359502/148‑1 (MQ=255) tCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGAt > 2:161300/1‑148 (MQ=255) tCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGAt > 1:343854/1‑148 (MQ=255) tCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGAt > 1:333936/1‑148 (MQ=255) ggATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGc > 4:487045/1‑149 (MQ=255) gATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCt > 4:770177/1‑149 (MQ=255) aTCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTg > 4:716220/1‑149 (MQ=255) gCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCg > 3:722296/1‑149 (MQ=255) ccTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCgg > 4:482688/1‑149 (MQ=255) ccTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCg > 2:582984/1‑148 (MQ=255) ttCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGc < 1:301687/148‑1 (MQ=255) gcggcggAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCa > 4:129932/1‑149 (MQ=255) gcggAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATc < 4:609231/149‑1 (MQ=255) gAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCtt < 1:797569/148‑1 (MQ=255) aTGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGtt < 4:904975/149‑1 (MQ=255) aTGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGtt < 3:706610/149‑1 (MQ=255) aTGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGtt < 3:487045/149‑1 (MQ=255) aTGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGtt < 3:312405/149‑1 (MQ=255) gTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCa < 3:770177/149‑1 (MQ=255) gTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCa < 3:482688/149‑1 (MQ=255) gTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCa < 3:415534/149‑1 (MQ=255) cgcgCACCAGACGTTCGAGAACGTCTTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTAGTTCAGt > 3:778751/1‑149 (MQ=255) gcgcACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTa > 4:357560/1‑149 (MQ=255) cgcACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCGATCTTGtt < 3:833609/143‑1 (MQ=255) cgcACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGtt > 4:833609/1‑143 (MQ=255) gcACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGt > 2:908431/1‑141 (MQ=255) gcACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGt < 1:908431/141‑1 (MQ=255) aggACGTTCTCGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGca > 4:77292/3‑149 (MQ=255) aggACGTTCTCGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGc > 1:146170/3‑148 (MQ=255) aGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGca > 2:589277/1‑148 (MQ=255) gACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGcaca > 3:249995/1‑149 (MQ=255) gACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGcac > 2:668771/1‑148 (MQ=255) aCGTTCTCGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGTGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGcaca > 2:167984/1‑148 (MQ=255) aCGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATttc < 2:145460/90‑1 (MQ=255) aCGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATttc > 1:145460/1‑90 (MQ=255) ttCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACtt > 3:89942/1‑149 (MQ=255) gagaACGTCCTGCACACCAACGCCGGTTTTCGCTGGACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAAATTcta < 4:89942/149‑3 (MQ=255) | TTTGTCGCCCTTACGCAGGGTGCCGTTTTTAATACGGATAAGTGAAACAACGCCCAGGTAGTTGTCGAACCATGAGTCGATAATTAGTGCCTGCAACGGGCCTTCCGGATCGCCTTCCGGCGGCGGAATGTCGCGCACCAGACGTTCGAGAACGTCCTGCACACCAACGCCGGTTTTCGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGA > NC_000913/2706459‑2706754 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |