Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 314,258 G→A 12.9% intergenic (‑453/‑99) ykgP ← / → eaeH pseudogene, oxidoreductase family/pseudogene, attaching and effacing protein homology;factor; Not classified

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913314,2580GA12.9% 255.7 / 24.8 93intergenic (‑453/‑99)ykgP/eaeHpseudogene, oxidoreductase family/pseudogene, attaching and effacing protein homology;factor; Not classified
Reads supporting (aligned to +/- strand):  ref base G (42/39);  new base A (6/6);  total (48/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

ATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTC  >  NC_000913/314115‑314403
                                                                                                                                               |                                                                                                                                                 
aTTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGaaaa                                                                                                                                              <  3:257853/149‑1 (MQ=255)
aTTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGaaa                                                                                                                                               <  1:471712/148‑1 (MQ=255)
aTTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGaaa                                                                                                                                               <  2:16155/148‑1 (MQ=255)
aTTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGaaa                                                                                                                                               <  2:805871/148‑1 (MQ=255)
aTTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCCGCAGaaa                                                                                                                                               <  1:448143/148‑1 (MQ=255)
 tttAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGaaaa                                                                                                                                              <  1:343886/148‑1 (MQ=255)
   tAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTTCATAAAGGTATTTCCTTTTCCGCAGAAAAGGa                                                                                                                                           <  3:592366/149‑1 (MQ=255)
   tAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCCGCAGAAAAGGa                                                                                                                                           <  3:6546/149‑1 (MQ=255)
    aaTGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCCGCAGAAAAGGaa                                                                                                                                          <  3:731095/149‑1 (MQ=255)
          ttGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCgg                                                                                                                                     >  3:225329/1‑148 (MQ=255)
          ttGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCgg                                                                                                                                     <  4:225329/148‑1 (MQ=255)
           tGCACAAAAAGCTAAACTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTTCGGAAAAGGAAATCggg                                                                                                                                    <  2:691695/148‑1 (MQ=255)
               caAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACCTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATc                                                                                                                               >  4:286195/1‑149 (MQ=255)
                  aaaGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATcccc                                                                                                                            >  3:708690/1‑149 (MQ=255)
                   aaGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATcccc                                                                                                                            >  1:876359/1‑148 (MQ=255)
                   aaGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCg                                                                                                                           <  4:341593/149‑1 (MQ=255)
                   aaGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCg                                                                                                                           >  4:805325/1‑149 (MQ=255)
                       tAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACTTAAAGGTATTTCCTTTTCCGCAGAAAAGGaa                                                                                                                                          <  1:447244/130‑1 (MQ=255)
                       tAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCCGCAGAAAAGGaa                                                                                                                                          >  2:447244/1‑130 (MQ=255)
                       tAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCCGCAGAAAAGGAAAtaccttt                                                                                                                                  <  4:420357/138‑7 (MQ=255)
                       tAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCCGCAGAAAAGGAAAtaccttt                                                                                                                                  >  3:420357/1‑132 (MQ=255)
                        aaaTTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGttt                                                                                                                       >  1:303926/1‑148 (MQ=255)
                          aTTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGttttt                                                                                                                     <  2:303926/148‑1 (MQ=255)
                                ccccATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACaa                                                                                                              >  4:121575/1‑149 (MQ=255)
                                  ccATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGc                                                                                                            <  3:678681/149‑1 (MQ=255)
                                  ccATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTAACAAGc                                                                                                            >  4:644206/1‑149 (MQ=255)
                                     taaataaaAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGa                                                                                                         >  3:869052/1‑149 (MQ=255)
                                        ataaaAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACaa                                                                                                              >  4:346349/1‑141 (MQ=255)
                                        ataaaAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACaa                                                                                                              <  3:346349/141‑1 (MQ=255)
                                            aaaTATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGAc                                                                                                                >  4:260163/1‑135 (MQ=255)
                                            aaaTATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGAc                                                                                                                <  3:260163/135‑1 (MQ=255)
                                            aaaTATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCatt                                                                                                  >  3:130897/1‑149 (MQ=255)
                                            aaaTATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCatt                                                                                                  >  4:189348/1‑149 (MQ=255)
                                              atatAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCattat                                                                                                >  3:890577/1‑149 (MQ=255)
                                              atatAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCattat                                                                                                <  3:121575/149‑1 (MQ=255)
                                               tataATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCattatt                                                                                               >  3:190815/1‑149 (MQ=255)
                                                  aaTCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTtgt                                                                                            <  4:890577/149‑1 (MQ=255)
                                                   aTCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCgg                                                                                                                                     <  3:901242/107‑1 (MQ=255)
                                                   aTCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCgg                                                                                                                                     >  4:901242/1‑107 (MQ=255)
                                                        gcgcCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGc                                                                                      <  4:869052/149‑1 (MQ=255)
                                                                  accTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGt                                                                            >  3:460582/1‑149 (MQ=255)
                                                                   ccTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATTAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGtt                                                                           <  3:644206/149‑1 (MQ=255)
                                                                      gatgaGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTATTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTaa                                                                         >  1:267394/1‑148 (MQ=255)
                                                                        tgaGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACCTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTc                                                                      <  3:286195/149‑1 (MQ=255)
                                                                           gTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGtt                                                                   <  4:460582/149‑1 (MQ=255)
                                                                            tGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTCCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTtc                                                                  <  3:288656/149‑1 (MQ=255)
                                                                               cTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCatta                                                                                                 <  4:690754/115‑1 (MQ=255)
                                                                               cTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCatta                                                                                                 >  3:690754/1‑115 (MQ=255)
                                                                                tataGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCAGCGGAAAAGGAAATCGGGTAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTtcatca                                                              >  4:419129/1‑149 (MQ=255)
                                                                                 ataGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAg                                                             >  3:588228/1‑149 (MQ=255)
                                                                                      cACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAAt                                                        >  4:830000/1‑149 (MQ=255)
                                                                                        cTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAAtt                                                       <  2:185666/148‑1 (MQ=255)
                                                                                          ggATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTCCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTAtc                                                    >  3:156296/1‑149 (MQ=255)
                                                                                           gATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTAtct                                                   >  3:724977/1‑149 (MQ=255)
                                                                                           gATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTAtct                                                   >  3:169192/1‑149 (MQ=255)
                                                                                             tatatTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCa                                                 >  3:86031/1‑149 (MQ=255)
                                                                                              atatTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCaa                                                >  4:257915/1‑149 (MQ=255)
                                                                                                 ttAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGt                                             >  3:629585/1‑149 (MQ=255)
                                                                                                  tAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGcc                                                                                     <  1:806885/108‑1 (MQ=255)
                                                                                                  tAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGcc                                                                                     >  2:806885/1‑108 (MQ=255)
                                                                                                  tAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGt                                             >  2:286320/1‑148 (MQ=255)
                                                                                                    ggTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCgg                                                                                                                          <  3:635731/69‑1 (MQ=255)
                                                                                                    ggTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCgg                                                                                                                          >  4:635731/1‑69 (MQ=255)
                                                                                                    ggTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTAGGTTCTTTAATTCGTTCATCAGGAAATTATCTCAATGTcac                                          >  4:587730/1‑149 (MQ=255)
                                                                                                      tGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTcaca                                         >  1:510257/1‑148 (MQ=255)
                                                                                                       ggCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACAtt                                       <  3:189348/149‑1 (MQ=255)
                                                                                                          ggTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATtata                                    <  3:875968/149‑1 (MQ=255)
                                                                                                           gTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATaa                                   >  4:5272/1‑149 (MQ=255)
                                                                                                             gCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATaaaa                                 <  4:130897/149‑1 (MQ=255)
                                                                                                              cACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAAc                                >  3:849467/1‑149 (MQ=255)
                                                                                                                cTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAg                              <  4:724977/149‑1 (MQ=255)
                                                                                                                 tttCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAgg                             <  3:257915/149‑1 (MQ=255)
                                                                                                                  ttCTTACATAAAGGTATTCCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGt                            <  4:156296/149‑1 (MQ=255)
                                                                                                                       aCATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAAc                                >  1:831680/1‑140 (MQ=255)
                                                                                                                       aCATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAAc                                <  2:831680/140‑1 (MQ=255)
                                                                                                                        cATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATaaa                      <  4:849467/149‑1 (MQ=255)
                                                                                                                           aaaGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAaacaa                   <  3:419129/149‑1 (MQ=255)
                                                                                                                            aaGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTCTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAaacaa                   >  2:275368/1‑148 (MQ=255)
                                                                                                                              ccgaTTTCCTTTTCCGCAGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCa                >  3:134457/4‑149 (MQ=255)
                                                                                                                                 aTTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTCTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACg              <  1:275368/148‑1 (MQ=255)
                                                                                                                                    tCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGAttt          >  3:8310/1‑149 (MQ=255)
                                                                                                                                    tCCTTTTCCGCAGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGAttt          >  4:258485/1‑149 (MQ=255)
                                                                                                                                    tCCTTTTCCGCAGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGAtt           >  2:321871/1‑148 (MQ=255)
                                                                                                                                    tCCTTTTCCGCAGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGAtt           >  2:883303/1‑148 (MQ=255)
                                                                                                                                      cTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGt                                                                    >  4:392810/1‑89 (MQ=255)
                                                                                                                                      cTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGt                                                                    <  3:392810/89‑1 (MQ=255)
                                                                                                                                      cTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAAc                                <  4:708995/125‑1 (MQ=255)
                                                                                                                                      cTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAAc                                >  3:708995/1‑125 (MQ=255)
                                                                                                                                       ttttCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAg                              <  3:885949/126‑1 (MQ=255)
                                                                                                                                       ttttCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAg                              >  4:885949/1‑126 (MQ=255)
                                                                                                                                       ttttCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCg        <  2:267394/148‑1 (MQ=255)
                                                                                                                                        tttCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGtt      <  3:805325/149‑1 (MQ=255)
                                                                                                                                            tGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTc  >  4:730775/1‑149 (MQ=255)
                                                                                                                                               ggAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGAtt           <  3:379889/137‑1 (MQ=255)
                                                                                                                                               ggAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGAtt           >  4:379889/1‑137 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
ATTTAATGACTTGCACAAAAAGCTAAATTTTCCCCCATAAATAAAAATATAATCCCGCGCCCAACCACCTGATGAGTGGCTATAGGCACTGGATATATTAGGTGGCGGTGCACTTTCTTACATAAAGGTATTTCCTTTTCTGCGGAAAAGGAAATCGGGAAATCCCCGGTTTTTCTGACAAGCAGACGCCATTATTTGTGTCTGCCTATGTTCGTTAATTCGTTCATCAGGAAATTATCTCAATGTCACATTATAAAACAGGTCATAAACAACCACGATTTCGTTATTC  >  NC_000913/314115‑314403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: