Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,771,180 G→T 5.0% L11L (CTG→CTT yfjT → CP4‑57 prophage; putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,771,1800GT 5.0% 422.1 / 9.6 119L11L (CTG→CTTyfjTCP4‑57 prophage; putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (64/49);  new base T (4/2);  total (68/51)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.89e-01

Only 100 of 121 total aligned reads displayed.

CCGTATACGAAAGAAAAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAA  >  NC_000913/2771032‑2771326
                                                                                                                                                    |                                                                                                                                                  
ccGTATACGAAAGAAAAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTg                                                                                                                                                    <  3:184567/149‑1 (MQ=255)
 cGTATACGAAAGAAAAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTg                                                                                                                                                    >  1:167776/1‑148 (MQ=255)
     taCGAAAGAAAAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAg                                                                                                                                               >  4:882035/1‑149 (MQ=255)
       cGAAAGAAAAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGt                                                                                                                                              >  1:109104/1‑148 (MQ=255)
        gaaagaaaAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTTGCCAGTAc                                                                                                                                            >  4:389766/1‑149 (MQ=255)
        gaaagaaaAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTa                                                                                                                                             <  1:647364/148‑1 (MQ=255)
            gaaaAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTTGCCAGTACaaac                                                                                                                                        <  3:389766/149‑3 (MQ=255)
               aaaTACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTc                                                                                                                                     <  3:237547/149‑1 (MQ=255)
                 aTACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGc                                                                                                                                   >  3:795161/1‑149 (MQ=255)
                        gAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCt                                                                                                                              >  4:321623/1‑147 (MQ=255)
                        gAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCt                                                                                                                              <  3:321623/147‑1 (MQ=255)
                           tatTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACa                                                                                                                         <  4:71722/149‑1 (MQ=255)
                           tatTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACa                                                                                                                         >  4:415463/1‑149 (MQ=255)
                             ttCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACaa                                                                                                                                          <  4:302867/130‑1 (MQ=255)
                             ttCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACaa                                                                                                                                          >  3:302867/1‑130 (MQ=255)
                               cccATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCct                                                                                                                     <  4:773702/149‑1 (MQ=255)
                                 cATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCctct                                                                                                                   <  3:494752/149‑1 (MQ=255)
                                   tGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGc                                                                                                                 <  3:882035/149‑1 (MQ=255)
                                    gACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGc                                                                                                                 >  1:821306/1‑148 (MQ=255)
                                         cAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTcc                                                                                                            <  2:252870/148‑1 (MQ=255)
                                            aCTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCgg                                                                                                        >  3:652679/1‑149 (MQ=255)
                                             cTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCgg                                                                                                        >  2:588570/1‑148 (MQ=255)
                                                 tGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGActc                                                                                                    <  1:141348/148‑1 (MQ=255)
                                                   ggACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTg                                                                                                  <  2:803733/148‑1 (MQ=255)
                                                   agACGGCTGCTGACACCGCCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGATCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTgg                                                                                                 >  4:784146/2‑149 (MQ=255)
                                                    gACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGAc                                                                                                      <  1:792525/143‑1 (MQ=255)
                                                    gACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGAc                                                                                                      >  2:792525/1‑143 (MQ=255)
                                                     aCGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGc                                                                                                >  1:821705/1‑148 (MQ=255)
                                                         ctgctgATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACa                                                                                            <  2:109104/148‑1 (MQ=255)
                                                         ctgctgATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAg                                                                                           >  3:575898/1‑149 (MQ=255)
                                                          tgctgATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAg                                                                                           <  2:398927/148‑1 (MQ=255)
                                                           gctgATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAggg                                                                                         <  4:278111/149‑1 (MQ=255)
                                                           gctgATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAgg                                                                                          <  2:849905/148‑1 (MQ=255)
                                                           gctgATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAgg                                                                                          <  2:693088/148‑1 (MQ=255)
                                                             tgATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGt                                                                                       >  4:884932/1‑149 (MQ=255)
                                                              gATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGt                                                                                       >  1:37815/1‑148 (MQ=255)
                                                               aTACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTa                                                                                      <  2:167776/148‑1 (MQ=255)
                                                                 aCCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACgg                                                                                   >  4:211988/1‑149 (MQ=255)
                                                                 aCCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACg                                                                                    <  2:37815/148‑1 (MQ=255)
                                                                     tCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAg                                                                               >  3:634958/1‑149 (MQ=255)
                                                                      cAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAg                                                                               >  2:457178/1‑148 (MQ=255)
                                                                           ccTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAAt                                                                         >  3:208568/1‑149 (MQ=255)
                                                                            cTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATg                                                                        <  4:634958/149‑1 (MQ=255)
                                                                            cTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATg                                                                        <  4:590521/149‑1 (MQ=255)
                                                                              gATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAgg                                                                                          >  3:575359/1‑129 (MQ=255)
                                                                              gATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAgg                                                                                          <  4:575359/129‑1 (MQ=255)
                                                                               aTAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCg                                                                      >  1:7574/1‑148 (MQ=255)
                                                                                   ccGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAAt                                                                         <  4:875988/141‑1 (MQ=255)
                                                                                   ccGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAAt                                                                         >  3:875988/1‑141 (MQ=255)
                                                                                    cGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATa                                                                 >  1:295080/1‑148 (MQ=255)
                                                                                      ggAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAg                                                              <  4:795161/149‑1 (MQ=255)
                                                                                       gAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAg                                                              <  1:286693/148‑1 (MQ=255)
                                                                                       gAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAg                                                              <  1:507603/148‑1 (MQ=255)
                                                                                           aaaaaCAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGa                                                         >  3:307857/1‑149 (MQ=255)
                                                                                             aaaCAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACg                                                       >  3:880401/1‑149 (MQ=255)
                                                                                             aaaCAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACg                                                       >  3:725244/1‑149 (MQ=255)
                                                                                             aaaCAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACg                                                       >  3:222670/1‑149 (MQ=255)
                                                                                              aacaacGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACgg                                                      >  4:479791/1‑149 (MQ=255)
                                                                                              aacaacGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACg                                                       >  1:863700/1‑148 (MQ=255)
                                                                                              aacaacGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACg                                                       >  2:370023/1‑148 (MQ=255)
                                                                                               acaacGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACgg                                                      <  1:457178/148‑1 (MQ=255)
                                                                                                caacGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGAt                                                    <  3:479791/149‑1 (MQ=255)
                                                                                                 aacGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATg                                                   >  3:19311/1‑149 (MQ=255)
                                                                                                 aacGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATg                                                   >  3:141035/1‑149 (MQ=255)
                                                                                                  acGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCg                                                                                                                <  1:723833/87‑1 (MQ=255)
                                                                                                  acGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCg                                                                                                                >  2:723833/1‑87 (MQ=255)
                                                                                                  acGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGc                                                  <  4:880401/149‑1 (MQ=255)
                                                                                                    gagaAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCa                                                >  3:877745/1‑149 (MQ=255)
                                                                                                      gaAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAg                                               <  2:863700/148‑1 (MQ=255)
                                                                                                         aGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTa                                                                                                                                             >  4:410700/1‑51 (MQ=255)
                                                                                                         aGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTa                                                                                                                                             <  3:410700/51‑1 (MQ=255)
                                                                                                           gagaCATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGaa                                          >  2:493241/1‑148 (MQ=255)
                                                                                                                aTAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGt                                    >  3:184723/1‑149 (MQ=255)
                                                                                                                 tAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACgg                                                      <  1:45259/130‑1 (MQ=255)
                                                                                                                 tAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGt                                    >  2:890958/1‑148 (MQ=255)
                                                                                                                    aTGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTAc                                <  4:141035/149‑1 (MQ=255)
                                                                                                                    aTGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTAACAGCCTCTGCGGTCCCCGGACTCTGGCAACa                                                                                            <  1:151968/89‑1 (MQ=255)
                                                                                                                    aTGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTAACAGCCTCTGCGGTCCCCGGACTCTGGCAACa                                                                                            >  2:151968/1‑89 (MQ=255)
                                                                                                                     tGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATAc                                                                >  1:126753/1‑116 (MQ=255)
                                                                                                                     tGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATAc                                                                <  2:126753/116‑1 (MQ=255)
                                                                                                                     tGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTAc                                >  1:327627/1‑148 (MQ=255)
                                                                                                                     tGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGACAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGAAAACAGTGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCTGAAAGATATTTAc                                >  2:856309/1‑148 (MQ=255)
                                                                                                                        aaaTACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCAt                             <  2:821306/148‑1 (MQ=255)
                                                                                                                          aTACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCa                           <  1:588570/148‑1 (MQ=255)
                                                                                                                           tACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGAGGTTTACCATCAAc                         >  3:167592/1‑149 (MQ=255)
                                                                                                                               ttCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGc                      >  2:230379/1‑148 (MQ=255)
                                                                                                                                   cTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACgg                  >  1:378549/1‑148 (MQ=255)
                                                                                                                                    tAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACggg                 >  1:868344/1‑148 (MQ=255)
                                                                                                                                     aaGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGa                >  1:908326/1‑148 (MQ=255)
                                                                                                                                       gCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGaaa              <  2:565775/148‑1 (MQ=255)
                                                                                                                                         aacattGTACTTGCCAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATc            >  1:537658/5‑148 (MQ=255)
                                                                                                                                           gTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACGGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCgg         >  3:44296/1‑149 (MQ=255)
                                                                                                                                           gTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCgg         <  4:819466/149‑1 (MQ=255)
                                                                                                                                           gTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCgg         <  3:784146/149‑1 (MQ=255)
                                                                                                                                            tttGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCgg         <  1:62902/148‑1 (MQ=255)
                                                                                                                                            tttGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGa        >  4:589278/1‑149 (MQ=255)
                                                                                                                                            attGTACTTGCCAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGa        >  4:552782/2‑149 (MQ=255)
                                                                                                                                                  cTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGaa  <  3:313004/149‑1 (MQ=255)
                                                                                                                                                   tGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGt                                    <  4:30919/114‑1 (MQ=255)
                                                                                                                                                   tGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGaa  >  2:869713/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CCGTATACGAAAGAAAAATACTTCGAATATTCCCATGACGGCAAACTGATGGGACGGCTGCTGATACCGTCAAACCCTGATAACCGGGAATAAAAACAACGAGAAAGGAGACATAAATGAAAATACGTTCACTAAGCCGGTTTGTACTGGCAAGTACAATGTTCGCCAGCTTTACAGCCTCTGCGGTCCCCGGACTCTGGCAACAGGGGTACGGTCAGGGCAATGCGGAATACAGTGTGACGGATGCCAGCGGAAAGATGTTTACCATCAACTGCACGGGAAATCCGGACCAGAA  >  NC_000913/2771032‑2771326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: