Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,078,186 C→T 7.4% G225D (GGC→GAC)  cmtA ← putative mannitol‑specific PTS IIB and IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,078,1860CT 7.4% 378.4 / 11.6 120G225D (GGC→GAC) cmtAputative mannitol‑specific PTS IIB and IIC components
Reads supporting (aligned to +/- strand):  ref base C (53/58);  new base T (6/3);  total (59/61)
Fisher's exact test for biased strand distribution p-value = 3.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 125 total aligned reads displayed.

TGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATG  >  NC_000913/3078040‑3078333
                                                                                                                                                  |                                                                                                                                                   
tGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGccc                                                                                                                                                   >  4:898740/1‑149 (MQ=255)
     ataatGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGAcc                                                                                                                                              >  3:778705/1‑149 (MQ=255)
        atGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTgg                                                                                                                                           <  4:711070/149‑1 (MQ=255)
              aGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTaga                                                                                                                                     <  4:213773/149‑1 (MQ=255)
              aGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTag                                                                                                                                      >  2:185170/1‑148 (MQ=255)
                cGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTagag                                                                                                                                    >  1:690242/1‑148 (MQ=255)
                cGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTagag                                                                                                                                    >  1:188422/1‑148 (MQ=255)
                cGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAgg                                                                                                                                   <  4:863351/149‑1 (MQ=255)
                 ggCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCAGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAgg                                                                                                                                   <  2:564660/148‑1 (MQ=255)
                           gCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATaaa                                                                                                                         >  3:295301/1‑148 (MQ=255)
                           gCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATaaa                                                                                                                         <  4:295301/148‑1 (MQ=255)
                             acaTACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAaaga                                                                                                                       >  2:627115/1‑148 (MQ=255)
                               aTACGGGAAATACAGTTCGTGGACCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAagaaga                                                                                                                    <  3:898740/149‑1 (MQ=255)
                                tACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGcc                                                                                                                                                    <  3:262283/116‑1 (MQ=255)
                                tACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAagaaga                                                                                                                    <  2:188422/148‑1 (MQ=255)
                                tACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGAt                                                                                                                   <  4:590418/149‑1 (MQ=255)
                                tACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCACGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCAGGGTTAGAGGCCACCATAAagaaga                                                                                                                    <  2:323525/148‑1 (MQ=255)
                                    ggAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGAt                                                                                                               <  3:334189/149‑1 (MQ=255)
                                         tACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTaga                                                                                                                                     <  2:113083/122‑1 (MQ=255)
                                         tACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTaga                                                                                                                                     >  1:113083/1‑122 (MQ=255)
                                           cAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCg                                                                                                         >  2:641902/1‑148 (MQ=255)
                                              ttCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTaa                                                                                                     >  3:630543/1‑149 (MQ=255)
                                              ttCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTaa                                                                                                     <  4:778705/149‑1 (MQ=255)
                                               tCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCt                                                                                                                                             >  1:410339/1‑108 (MQ=255)
                                               tCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCt                                                                                                                                             <  2:410339/108‑1 (MQ=255)
                                                   ggATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGa                                                                                                                <  1:49335/133‑1 (MQ=255)
                                                   ggATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGa                                                                                                                >  2:49335/1‑133 (MQ=255)
                                                    gATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGaa                                                                                                >  1:187606/1‑148 (MQ=255)
                                                        ccACCGAGGAGGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCt                                                                                            >  1:476226/1‑148 (MQ=255)
                                                         cACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAATCAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTg                                                                                           <  1:653285/148‑1 (MQ=255)
                                                         cACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGt                                                                                          >  4:898681/1‑149 (MQ=255)
                                                          aCCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGtt                                                                                         >  3:477250/1‑149 (MQ=255)
                                                              aGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCt                                                                                            <  4:9000/142‑1 (MQ=255)
                                                              aGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCt                                                                                            >  3:9000/1‑142 (MQ=255)
                                                                        taatCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGccac                                                                                                                               >  3:332831/1‑97 (MQ=255)
                                                                        taatCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGccac                                                                                                                               <  4:332831/97‑1 (MQ=255)
                                                                         aatCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGat                                                                          <  3:293414/149‑1 (MQ=255)
                                                                          atcatcGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGata                                                                         >  4:588257/1‑149 (MQ=255)
                                                                          atcatcGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGat                                                                          >  1:848444/1‑148 (MQ=255)
                                                                            catcGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGataat                                                                       <  3:588257/149‑1 (MQ=255)
                                                                            catcGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGataa                                                                        >  2:110646/1‑148 (MQ=255)
                                                                             atcGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGataat                                                                       <  2:386180/148‑1 (MQ=255)
                                                                              tcGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGataata                                                                      >  2:123701/1‑148 (MQ=255)
                                                                                gCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGaa                                                                                                >  3:410219/1‑120 (MQ=255)
                                                                                gCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGaa                                                                                                <  4:410219/120‑1 (MQ=255)
                                                                                         gcAGAACGTTTACTCATCTCTTTACCAAACAAGGTAACCGCCAGCAGCAGTCCCAGGCCCGGTCTTGGGATAAAGGCCACCATAAAGAAGTTGGACTTACCGTTAACCGAAGCCTGTTGCATTCCCAACGGAAAATAGACGCCCTgaac                                                          >  4:833143/1‑147 (MQ=255)
                                                                                         gcAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTgatc                                                          >  3:874643/1‑149 (MQ=255)
                                                                                           aGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTgatcg                                                         <  1:123701/148‑1 (MQ=255)
                                                                                                   tACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCatta                                                <  3:243637/149‑1 (MQ=255)
                                                                                                      tCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTgatcgat                                                       <  4:73505/139‑1 (MQ=255)
                                                                                                       cATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATg                                                                                                                  >  4:231112/1‑79 (MQ=255)
                                                                                                       cATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATg                                                                                                                  <  3:231112/79‑1 (MQ=255)
                                                                                                       cATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTg                                             >  2:361243/1‑148 (MQ=255)
                                                                                                       cATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTg                                             >  1:852736/1‑148 (MQ=255)
                                                                                                       cATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTg                                             <  2:690242/148‑1 (MQ=255)
                                                                                                       cATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTg                                             <  2:719265/148‑1 (MQ=255)
                                                                                                       cATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGa                                            <  3:459637/149‑1 (MQ=255)
                                                                                                        aTCCCTTTACCAAACAAGGTGAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGa                                            <  2:718214/148‑1 (MQ=255)
                                                                                                        aTCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGa                                            <  1:627115/148‑1 (MQ=255)
                                                                                                        aTCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGa                                            <  1:185170/148‑1 (MQ=255)
                                                                                                             tttACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGaaaa                                      >  3:536008/1‑149 (MQ=255)
                                                                                                             tttACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGaaa                                       >  2:621548/1‑148 (MQ=255)
                                                                                                             ggtACCAAACGAAGTAAGCGCCAGCACCAGCCCCCGGCCCGGACCTGGGTTAGCGGCCCCCGTAGGGAAGATGCATTTACCGTTAACCGGCGCCTCTTGCAGTCCCAGCGGATAATAGACGCCCTGATCGCGCGCATTATTGAGGaaaa                                      <  4:480257/147‑1 (MQ=255)
                                                                                                              ttACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGaaaa                                      >  1:104900/1‑148 (MQ=255)
                                                                                                               tACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGc                                    <  3:85779/149‑1 (MQ=255)
                                                                                                                 ccAAACAAGGTAAACGCCAGCAGCAGCTCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCAc                                  >  4:873261/1‑149 (MQ=255)
                                                                                                                  cAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAAc                                                                                                    <  3:421553/82‑1 (MQ=255)
                                                                                                                  cAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAAc                                                                                                    >  4:421553/1‑82 (MQ=255)
                                                                                                                    aaCAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTgat                                                           >  4:26328/1‑121 (MQ=255)
                                                                                                                    aaCAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTg                                             <  1:710429/135‑1 (MQ=255)
                                                                                                                    aaCAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACtt                                >  1:692200/1‑148 (MQ=255)
                                                                                                                    aaCAAGGTAAACGCCAGAAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTg                                             >  2:710429/1‑135 (MQ=255)
                                                                                                                     aCAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCAAttt                               <  1:621548/148‑1 (MQ=255)
                                                                                                                            aaaCGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGt                        >  2:483532/1‑148 (MQ=255)
                                                                                                                            aaaCGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGt                        <  2:626357/148‑1 (MQ=255)
                                                                                                                              aCGCCAGCAGCCGCCCCAGGCCCGGCCCTGGGTTAGCGGCCACCATAAAGAAGGTGGATGTTCCGTTGACCGAAGCCTGTTGCATTCCCAGCGGATAATGGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTc                      >  2:521505/1‑148 (MQ=255)
                                                                                                                                gCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATCGGCGTCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCAt                    <  1:319264/148‑1 (MQ=255)
                                                                                                                                  cagcagcagCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATtga                 <  4:502437/149‑1 (MQ=255)
                                                                                                                                  cagcagcagCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATtg                  >  2:128894/1‑148 (MQ=255)
                                                                                                                                      agcagcCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATtgatga              <  1:361243/148‑1 (MQ=255)
                                                                                                                                      agcagcCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGAtgac             <  4:360645/149‑1 (MQ=255)
                                                                                                                                      agcagcCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGAtgac             <  4:891314/149‑1 (MQ=255)
                                                                                                                                       tctaacccAGGTCCGGGCCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGAtgact            >  3:839288/6‑149 (MQ=255)
                                                                                                                                       gcagcCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGAtgact            <  3:756261/149‑1 (MQ=255)
                                                                                                                                        ctaacccAGGTCCGGGCCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATtg                  <  2:847974/138‑1 (MQ=255)
                                                                                                                                        ctaacccAGGTCCGGGCCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATtg                  >  1:847974/5‑142 (MQ=255)
                                                                                                                                         agcCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGAtgactg           <  2:104900/148‑1 (MQ=255)
                                                                                                                                            cccAGGTCCGGGCCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATtga                 <  4:118290/139‑1 (MQ=255)
                                                                                                                                            cccAGGTCCGGGCCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGaca        >  1:761592/1‑148 (MQ=255)
                                                                                                                                            cccAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGc                            <  4:198208/128‑1 (MQ=255)
                                                                                                                                            cccAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGc                            >  3:198208/1‑128 (MQ=255)
                                                                                                                                            cccAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGacaa       <  3:898681/149‑1 (MQ=255)
                                                                                                                                              cAGGTCCGGGCCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGACGAAAAGCAc                                  >  2:844074/1‑120 (MQ=255)
                                                                                                                                              cAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGacaac      >  1:86390/1‑148 (MQ=255)
                                                                                                                                              cAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGacaac      >  1:202090/1‑148 (MQ=255)
                                                                                                                                                 gCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAAt   <  2:848444/148‑1 (MQ=255)
                                                                                                                                                 gCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAAt   <  2:187606/148‑1 (MQ=255)
                                                                                                                                                 gCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATg  <  3:577447/149‑1 (MQ=255)
                                                                                                                                                  cccGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATg  <  2:835686/148‑1 (MQ=255)
                                                                                                                                                  cccGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATg  <  1:110646/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TGGCAATAATGGTCAGCGGCTTCATCAGCACATACGGGAAATACAGTTCGTGGATCCCACCGAGGAAGTGAATAATCATCGCCCCGGGCGCAGAACGTTTACTCATCCCTTTACCAAACAAGGTAAACGCCAGCAGCAGCCCCAGGCCCGGACCTGGGTTAGAGGCCACCATAAAGAAGATGGATTTACCGTTAACCGAAGCCTGTTGCATTCCCAGCGGATAATAGACGCCCTGATCGATCGCATTATTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATG  >  NC_000913/3078040‑3078333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: