Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,313,208 T→A 10.9% intergenic (‑30/+134) rbfA ← / ← infB 30s ribosome binding factor/translation initiation factor IF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,313,2080TA10.9% 231.0 / 16.7 73intergenic (‑30/+134)rbfA/infB30s ribosome binding factor/translation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base T (35/30);  new base A (5/3);  total (40/33)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

CGGAAACGGTGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAG  >  NC_000913/3313061‑3313346
                                                                                                                                                   |                                                                                                                                          
cGGAAACGGTGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCAATAATAAATTCTCCTGACAAAAAAGGGGCTGtt                                                                                                                                           >  4:595416/1‑149 (MQ=255)
  gAAACGGTGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAg                                                                                                                                         >  4:223771/1‑149 (MQ=255)
   aaaCGGTGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGc                                                                                                                                        <  4:139018/149‑1 (MQ=255)
       ggtggtCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGcccc                                                                                                                                     <  2:561808/148‑1 (MQ=255)
        gtggtCATCATGCCCAGGCGAGGATCTTTAATTTCACTCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCtt                                                                                                                                   <  4:10551/149‑1 (MQ=255)
          ggtCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCttt                                                                                                                                  >  1:21386/1‑148 (MQ=255)
          ggtCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTAACAGCCCCttt                                                                                                                                  <  1:6649/148‑1 (MQ=255)
           gtCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCttttt                                                                                                                                >  4:627208/1‑149 (MQ=255)
            tcatcaTGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTa                                                                                                                               >  3:446809/1‑149 (MQ=255)
            tcatcaTGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTa                                                                                                                               >  4:295722/1‑149 (MQ=255)
               tcatGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTaaaa                                                                                                                            >  4:504994/1‑149 (MQ=255)
               tcatGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTaaa                                                                                                                             <  2:757874/148‑1 (MQ=255)
                       aGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTc                                                                                                                    <  3:504994/149‑1 (MQ=255)
                        ggCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCa                                                                                                                   <  4:446809/149‑1 (MQ=255)
                         gCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAACATTAATTTCAg                                                                                                                  <  3:513753/149‑1 (MQ=255)
                            aGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTg                                                                                                               <  3:295722/149‑1 (MQ=255)
                               aTCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGa                                                                                                             <  1:760197/148‑1 (MQ=255)
                                tCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGaa                                                                                                            >  1:371885/1‑148 (MQ=255)
                                tCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAg                                                                                                           <  4:187683/149‑1 (MQ=255)
                                tCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATCTCAGGTGGAAg                                                                                                           <  3:595416/149‑1 (MQ=255)
                                  tttAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAATGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAggt                                                                                                         >  3:79976/1‑148 (MQ=255)
                                  tttAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAggg                                                                                                         >  3:725349/1‑149 (MQ=255)
                                  tttAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTAACAGCCCCtttttt                                                                                                                               <  4:815762/127‑2 (MQ=255)
                                  tttAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTAACAGCCCCtttttt                                                                                                                               >  3:815762/1‑126 (MQ=255)
                                   ttAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGTTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAggg                                                                                                         >  1:629001/1‑148 (MQ=255)
                                   ttAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAggg                                                                                                         >  2:626398/1‑148 (MQ=255)
                                   ttAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTAACAGCCCCttt                                                                                                                                  <  1:699478/123‑1 (MQ=255)
                                   ttAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTAACAGCCCCttt                                                                                                                                  >  2:699478/1‑123 (MQ=255)
                                       tttCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAg                                                                                                           <  4:110930/142‑1 (MQ=255)
                                       tttCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAg                                                                                                           >  3:110930/1‑142 (MQ=255)
                                       tttCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGt                                                                                                     <  2:21386/148‑1 (MQ=255)
                                        ttCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGtt                                                                                                    >  2:242753/1‑148 (MQ=255)
                                                     aTGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGa                                                                                       >  1:419849/1‑148 (MQ=255)
                                                     aTGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGAt                                                                                      <  4:79976/149‑1 (MQ=255)
                                                          agCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGa                                                                                  <  2:42089/148‑1 (MQ=255)
                                                                         cATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATaa                                                                                    <  3:819483/131‑1 (MQ=255)
                                                                         cATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATaa                                                                                    >  4:819483/1‑131 (MQ=255)
                                                                         cATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTcaca                                                                   >  2:829866/1‑148 (MQ=255)
                                                                           tttCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATc                                                                 >  1:99872/1‑148 (MQ=255)
                                                                           tttCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCa                                                                >  4:95294/1‑149 (MQ=255)
                                                                            ttCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAg                                                               <  4:725349/149‑1 (MQ=255)
                                                                                tGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCa                                                            <  1:242753/148‑1 (MQ=255)
                                                                                 gcgcTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCaa                                                           <  2:99872/148‑1 (MQ=255)
                                                                                          gcTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCg                                                  <  2:419849/148‑1 (MQ=255)
                                                                                               gggCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGc                                             <  2:371885/148‑1 (MQ=255)
                                                                                                 gCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGc                                          <  3:223771/149‑1 (MQ=255)
                                                                                                      cAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTTACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTaaa                                     >  3:348986/1‑149 (MQ=255)
                                                                                                      cAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGa                                                                                            <  3:511137/94‑1 (MQ=255)
                                                                                                      cAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGa                                                                                            >  4:511137/1‑94 (MQ=255)
                                                                                                              ttCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATATGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTa                              >  1:655456/1‑148 (MQ=255)
                                                                                                              ttCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTa                              >  2:637839/1‑148 (MQ=255)
                                                                                                                  ccATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATccc                          >  1:462937/1‑148 (MQ=255)
                                                                                                                  ccATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATccc                          >  1:665795/1‑148 (MQ=255)
                                                                                                                   cATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTaa                                      >  2:560583/1‑135 (MQ=255)
                                                                                                                   cATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTaa                                      <  1:560583/135‑1 (MQ=255)
                                                                                                                     taataaATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGAATGCAGCGTAAACGCCTTATCCCGCAt                      >  4:673405/1‑149 (MQ=255)
                                                                                                                       ataaATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATgg                    >  3:416484/1‑149 (MQ=255)
                                                                                                                         aaaTTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGa                   >  1:207043/1‑148 (MQ=255)
                                                                                                                            ttCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGAATGCAGCGTAAACGCCTTATCCCGCATGGAAccc               <  3:673405/149‑1 (MQ=255)
                                                                                                                               tcCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGa                                                                                  >  4:420598/1‑79 (MQ=255)
                                                                                                                               tcCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGa                                                                                  <  3:420598/79‑1 (MQ=255)
                                                                                                                                 cTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTaaaa          >  4:27482/1‑149 (MQ=255)
                                                                                                                                   gACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTaaaaa         >  1:828324/1‑148 (MQ=255)
                                                                                                                                    aCAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAAcc       <  3:27482/149‑1 (MQ=255)
                                                                                                                                    aCAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAAc        >  2:790173/1‑148 (MQ=255)
                                                                                                                                      aaaaaaGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCt      <  1:829866/148‑1 (MQ=255)
                                                                                                                                       aaaaaGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGaa                                                                                                            >  2:277281/1‑45 (MQ=255)
                                                                                                                                       aaaaaGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGaa                                                                                                            <  1:277281/45‑1 (MQ=255)
                                                                                                                                       aaaaaGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTa    >  4:483900/1‑149 (MQ=255)
                                                                                                                                        aaaaGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTaa   <  3:627208/149‑1 (MQ=255)
                                                                                                                                        aaaaGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGCGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGCCAATCCATGCTGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTa    >  2:574373/1‑148 (MQ=255)
                                                                                                                                        aaaaGGGGCTAACAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTa    >  1:223404/1‑148 (MQ=255)
                                                                                                                                          aaGGGGCTAACAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACCTCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAg  >  1:263376/1‑148 (MQ=255)
                                                                                                                                          aaGGGGCTAACAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAg  >  1:906704/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                          
CGGAAACGGTGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGGGGCTGTTAGCCCCTTTTTAAAATTAATTTCAGGTGGAAGGGCTGTTCACGTTGACCTGATAAGACGCGCCAGCGTCACATCAGGCAATCCATGCCGGATGCAGCGTAAACGCCTTATCCCGCATGGAACCCTAAAAACCTTAAG  >  NC_000913/3313061‑3313346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑