Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,387,553 T→A 6.8% R212R (CGA→CGT aaeB ← p‑hydroxybenzoic acid efflux system component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,387,5530TA 6.8% 296.4 / 6.3 85R212R (CGA→CGTaaeBp‑hydroxybenzoic acid efflux system component
Reads supporting (aligned to +/- strand):  ref base T (43/36);  new base A (3/3);  total (46/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTATTCTGAATAAGATAAGTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAAGAG  >  NC_000913/3387406‑3387693
                                                                                                                                                   |                                                                                                                                             
cgTATTCTGAATAAGATAAGTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTc                                                                                                                                              <  3:136705/149‑1 (MQ=255)
     tCTGAATAAGATAAGTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCAc                                                                                                                                          >  2:612992/1‑148 (MQ=255)
       tGAATAAGATAAGTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCa                                                                                                                                        <  2:284917/148‑1 (MQ=255)
       tGAATAAGATAAGTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAg                                                                                                                                       >  3:703888/1‑149 (MQ=255)
        gAATAAGATAAGTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGACGCACCacg                                                                                                                                      <  3:888158/149‑3 (MQ=255)
             agataagTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGACCTGGTGCGACGCACCACGgcgc                                                                                                                                  <  1:632569/148‑1 (MQ=255)
              gataagTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGccc                                                                                                                               >  4:405073/1‑149 (MQ=255)
               ataagTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCa                                                                                                                                        <  4:340666/140‑1 (MQ=255)
               ataagTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCa                                                                                                                                        >  3:340666/1‑140 (MQ=255)
                  agTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAgg                                                                                                                           >  4:777918/1‑149 (MQ=255)
                   gTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGc                                                                                                                          >  4:324000/1‑149 (MQ=255)
                         cAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTAt                                                                                                                     >  1:446537/1‑148 (MQ=255)
                            gATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGAc                                                                                                                 >  3:672833/1‑149 (MQ=255)
                              ttGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACa                                                                                                                <  2:382886/148‑1 (MQ=255)
                                    aaTCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCa                                                                                                          >  2:342248/1‑148 (MQ=255)
                                        agcgtcagcgATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATc                                                                                                     >  3:771003/1‑149 (MQ=255)
                                          cgtcagcgATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCg                                                                                                    >  1:186579/1‑148 (MQ=255)
                                            tcagcgATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATc                                                                                                                    >  3:268612/1‑130 (MQ=255)
                                            tcagcgATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATc                                                                                                                    <  4:268612/130‑1 (MQ=255)
                                            tcagcgATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCa                                                                                                 <  3:777918/149‑1 (MQ=255)
                                             cagcgATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCa                                                                                                 >  1:740457/1‑148 (MQ=255)
                                                   tAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTg                                                                                           >  2:678540/1‑148 (MQ=255)
                                                   tAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGa                                                                                          <  4:179562/149‑1 (MQ=255)
                                                     gCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATa                                                                                        >  4:375953/1‑149 (MQ=255)
                                                      cGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGAt                                                                                         <  1:859668/147‑1 (MQ=255)
                                                      cGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGAt                                                                                         >  2:859668/1‑147 (MQ=255)
                                                             aTCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTg                                                                                >  4:355454/1‑149 (MQ=255)
                                                              tCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGccc                                                                                                                               >  3:681213/1‑101 (MQ=255)
                                                              tCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGccc                                                                                                                               <  4:681213/101‑1 (MQ=255)
                                                              tCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCTCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGc                                                                               >  4:758558/1‑149 (MQ=255)
                                                              tCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTg                                                                                >  2:250961/1‑148 (MQ=255)
                                                              tCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTg                                                                                >  1:493333/1‑148 (MQ=255)
                                                              tCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTg                                                                                >  2:710576/1‑148 (MQ=255)
                                                               cGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGc                                                                               <  2:493333/148‑1 (MQ=255)
                                                                gCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCa                                                                              <  2:793263/148‑1 (MQ=255)
                                                                   tttAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTaa                                                                          <  3:405073/149‑1 (MQ=255)
                                                                       aaCGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGa                                                                      <  3:375953/149‑1 (MQ=255)
                                                                        aCGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGat                                                                     <  3:544228/149‑1 (MQ=255)
                                                                        aCGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGCTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACa                                                                                      >  4:489373/1‑132 (MQ=255)
                                                                        aCGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGCTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACa                                                                                      <  3:489373/132‑1 (MQ=255)
                                                                           tCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGatat                                                                   >  2:92129/1‑148 (MQ=255)
                                                                             gATTGGCCCGCGCCGAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTgc                                                                >  3:63882/1‑149 (MQ=255)
                                                                              aTTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTgc                                                                >  2:267342/1‑148 (MQ=255)
                                                                               ttGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTgcgc                                                              >  4:743809/1‑149 (MQ=255)
                                                                                    ccGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGatat                                                                   <  1:806282/139‑1 (MQ=255)
                                                                                    ccGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGatat                                                                   >  2:806282/1‑139 (MQ=255)
                                                                                     cgcgCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCa                                                         >  1:758947/1‑148 (MQ=255)
                                                                                      gcgcCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGc                                                       <  3:599551/149‑1 (MQ=255)
                                                                                         cccAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCaaa                                                    <  3:303234/149‑1 (MQ=255)
                                                                                          ccAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAAc                                                   >  4:721126/1‑149 (MQ=255)
                                                                                           cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTgcgc                                                              <  3:681001/137‑1 (MQ=255)
                                                                                           cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTgcgc                                                              >  4:681001/1‑137 (MQ=255)
                                                                                           cAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTCGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAAc                                                   >  2:155445/1‑148 (MQ=255)
                                                                                              cGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACAAGCAAACTTTc                                               <  4:771003/149‑1 (MQ=255)
                                                                                                ggAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCa                                             >  3:184120/1‑149 (MQ=255)
                                                                                                 gAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCa                                             <  2:273431/148‑1 (MQ=255)
                                                                                                   aGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGct                                          >  4:331221/1‑149 (MQ=255)
                                                                                                    gATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGct                                          <  1:92129/148‑1 (MQ=255)
                                                                                                      ttCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCtctc                                       >  3:20173/1‑149 (MQ=255)
                                                                                                           tatTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATcc                                  >  3:58745/1‑149 (MQ=255)
                                                                                                           tatTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATcc                                  >  4:549772/1‑149 (MQ=255)
                                                                                                              tCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTcc                                              >  2:80299/1‑134 (MQ=255)
                                                                                                              tCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTcc                                              <  1:80299/134‑1 (MQ=255)
                                                                                                                 ggTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCActtct                            <  3:324000/149‑1 (MQ=255)
                                                                                                                 ggTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCActtc                             <  1:710576/148‑1 (MQ=255)
                                                                                                                    tgctgcGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGt                         <  4:703888/149‑1 (MQ=255)
                                                                                                                     gctgcGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGt                         <  1:612992/148‑1 (MQ=255)
                                                                                                                         cgcATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTgat                    <  3:331221/149‑1 (MQ=255)
                                                                                                                               ccTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGttt                       <  4:645686/140‑1 (MQ=255)
                                                                                                                               ccTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGttt                       >  3:645686/1‑140 (MQ=255)
                                                                                                                                 ttGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATcgc             >  1:455964/1‑148 (MQ=255)
                                                                                                                                     ggcgacctggtgCGACGCACCACGGCGCTACAAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGga          >  2:423396/8‑148 (MQ=38)
                                                                                                                                      gcgcCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGaa       >  3:274917/1‑149 (MQ=255)
                                                                                                                                        gcCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGaaaa     <  3:721126/149‑1 (MQ=255)
                                                                                                                                        gcCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTCGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGaaa      <  1:155445/148‑1 (MQ=255)
                                                                                                                                         ccGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGaaaa     >  2:148610/1‑148 (MQ=255)
                                                                                                                                         acctggtgCGACGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCaaa                                                    <  3:633553/98‑1 (MQ=255)
                                                                                                                                         acctggtgCGACGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCaaa                                                    >  4:633553/4‑101 (MQ=255)
                                                                                                                                          cctggtgCGACGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAaga   >  4:431760/3‑149 (MQ=255)
                                                                                                                                          cGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAaga   <  4:672833/149‑1 (MQ=255)
                                                                                                                                          cGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAaga   <  4:274917/149‑1 (MQ=255)
                                                                                                                                          cGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAag    <  1:771842/148‑1 (MQ=255)
                                                                                                                                           gtggtgCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAagag  <  3:355454/149‑1 (MQ=255)
                                                                                                                                                gCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAaga   <  3:504640/143‑1 (MQ=255)
                                                                                                                                                gCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAaga   >  4:504640/1‑143 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
CGTATTCTGAATAAGATAAGTTTCGCAGGATTGGGTAATCAGCGTCAGCGATAGCGTATTGATCGCTTTTAAACGTCGATTGGCCCGCGCCCAGCGGGAAGATTCCATATTCAGGTTGCTGCGCATGCCTTGTAGCGCCGTGGTGCGTCGCACCAGGTCGCCCCAGGCTTTATCGACAACTTCACCATCGCCATGCTTGATACAGAGTTGCATTAATTGATATTGCGCGACCAGCAAACTTTCCAGCTCTCGATCCACTTCTTGTTTGATCGATCGCGGAGAAAAGAG  >  NC_000913/3387406‑3387693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: