Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 403,187 G→A 7.4% intergenic (+25/‑94) phoA → / → psiF bacterial alkaline phosphatase/PsiF family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913403,1870GA 7.4% 304.3 / 10.0 95intergenic (+25/‑94)phoA/psiFbacterial alkaline phosphatase/PsiF family protein
Reads supporting (aligned to +/- strand):  ref base G (36/52);  new base A (2/5);  total (38/57)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

ACAAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTACTGGTTACCTTGCTTTTCGGTCTGGTTTTTTTAACCA  >  NC_000913/403039‑403332
                                                                                                                                                    |                                                                                                                                                 
acaAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCg                                                                                                                                                   >  4:853480/1‑149 (MQ=255)
 caAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCg                                                                                                                                                   >  2:738162/1‑148 (MQ=255)
  aaGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGc                                                                                                                                                  <  2:768759/148‑1 (MQ=255)
  aaGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCt                                                                                                                                                 >  3:748515/1‑149 (MQ=255)
     aaCATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACTGACCAGACCGATCTCTTCAGCACCATGAAAGCCGCTCTGGGGCTGAAATAAAACTGCGCCCGGCAGCGAAAATTCgccgcc                                                                                                                                              <  4:573511/149‑1 (MQ=255)
     aaCATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGCGAAAATTCACTGcc                                                                                                                                              <  4:820281/149‑1 (MQ=255)
     aaCATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGCGAAAATTCACTGcc                                                                                                                                              <  3:177602/149‑1 (MQ=255)
      aCATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCg                                                                                                                                             >  3:490597/1‑149 (MQ=255)
      aCATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCg                                                                                                                                             >  3:1284/1‑149 (MQ=255)
         tACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCggg                                                                                                                                           >  1:332081/1‑148 (MQ=255)
         tACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGggt                                                                                                                                          >  3:351955/1‑149 (MQ=255)
         tACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGggt                                                                                                                                          <  4:627673/149‑1 (MQ=255)
         tACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGCGAAAATTCACTGCCg                                                                                                                                             <  4:747396/146‑1 (MQ=255)
         tACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGCGAAAATTCACTGCCg                                                                                                                                             >  3:747396/1‑146 (MQ=255)
         tACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAAC‑‑CACCCGGCAGCGAAAATTCACTGCCgggcg                                                                                                                                         <  1:435584/148‑3 (MQ=38)
            cGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAGACCGCGCCCGGCAGTGAATTTTCGCTGCCGggtggt                                                                                                                                       >  4:612293/1‑149 (MQ=255)
             ggCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAAC‑‑CACCCGGCAGCGAAAATTCACTGCCgggcgcggtt                                                                                                                                    <  4:230134/149‑8 (MQ=37)
                 gtcagtTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGttttt                                                                                                                                   <  2:333258/148‑1 (MQ=255)
                  tcagtTGCGTATTGCGGCGTATGGCCCGCATTCCGCCAATGATGTTGGACTGACCGACCAGACCGATCTCCTCTACACCATGAAAGACGCTCTGGGGCTGAAATATAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGttttttt                                                                                                                                 <  3:756940/149‑1 (MQ=255)
                  tcagtTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGttttttt                                                                                                                                 <  3:385248/149‑1 (MQ=255)
                  tcagtTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGtttttt                                                                                                                                  <  1:522476/148‑1 (MQ=255)
                      ttGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTg                                                                                                                             >  4:901532/1‑149 (MQ=255)
                         cGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTa                                                                                                                          <  3:99948/149‑1 (MQ=255)
                         cGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTa                                                                                                                          <  3:853480/149‑1 (MQ=255)
                         cGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTa                                                                                                                          >  4:842977/1‑149 (MQ=255)
                                  gcgTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGa                                                                                                                 <  4:748515/149‑1 (MQ=255)
                                  gcgTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGa                                                                                                                 >  4:512352/1‑149 (MQ=255)
                                     tATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACt                                                                                                               >  2:355599/1‑148 (MQ=255)
                                        ggCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTaa                                                                                                            <  2:436198/148‑1 (MQ=255)
                                        ggCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAAt                                                                                                           <  3:498140/149‑1 (MQ=255)
                                         gCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATg                                                                                                          >  3:713111/1‑149 (MQ=255)
                                          cccGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATg                                                                                                          <  1:308282/148‑1 (MQ=255)
                                            cGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTg                                                                                                                                <  2:443682/124‑1 (MQ=255)
                                            cGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTg                                                                                                                                >  1:443682/1‑124 (MQ=255)
                                            cGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGc                                                                                                        >  2:490376/1‑148 (MQ=255)
                                            cGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCa                                                                                                       <  3:612293/149‑1 (MQ=255)
                                              cATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGa                                                                                                     <  4:351955/149‑1 (MQ=255)
                                                           ttgttgGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCAt                                                                                         <  2:332081/148‑1 (MQ=255)
                                                              ttgGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGttttttt                                                                                                                                 <  4:382020/105‑1 (MQ=255)
                                                              ttgGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGttttttt                                                                                                                                 >  3:382020/1‑105 (MQ=255)
                                                              ttgGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACg                                                                                      <  1:490376/148‑1 (MQ=255)
                                                               tgGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGttt                                                                                                                                     >  1:781154/1‑100 (MQ=255)
                                                               tgGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGCGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGttt                                                                                                                                     <  2:781154/100‑1 (MQ=255)
                                                                 gACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTc                                                                                   <  2:479211/148‑1 (MQ=255)
                                                                   cTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCAt                                                                                 <  1:58672/148‑1 (MQ=255)
                                                                   cTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCAt                                                                                 <  1:395607/148‑1 (MQ=255)
                                                                       ccgaccAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGtt                                                                             >  2:677521/1‑148 (MQ=255)
                                                                          accAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTaaaa                                                                         >  4:121736/1‑149 (MQ=255)
                                                                          accAGACCGAGCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTaaa                                                                          <  1:303017/148‑1 (MQ=255)
                                                                              gACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACAt                                                                      >  1:839294/1‑148 (MQ=255)
                                                                               aCCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGa                                                                    >  4:6712/1‑149 (MQ=255)
                                                                                ccGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGa                                                                    <  1:763926/148‑1 (MQ=255)
                                                                                  gATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAag                                                                  >  2:341437/1‑148 (MQ=255)
                                                                                   aTCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAagag                                                                >  4:580064/1‑149 (MQ=255)
                                                                                   aTCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAagag                                                                <  3:580064/149‑1 (MQ=255)
                                                                                    tctcTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAgg                                                               >  4:659132/1‑149 (MQ=255)
                                                                                        ttctACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGAt                                                            >  1:150348/1‑148 (MQ=255)
                                                                                        ttctACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATg                                                           <  4:221732/149‑1 (MQ=255)
                                                                                         tctACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCa                                                                                  <  1:866508/125‑1 (MQ=255)
                                                                                         tctACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCa                                                                                  >  2:866508/1‑125 (MQ=255)
                                                                                         tctACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATg                                                           >  2:623188/1‑148 (MQ=255)
                                                                                          ctACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGt                                                         <  4:606562/149‑1 (MQ=255)
                                                                                             cacCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGc                                                       <  1:623188/148‑1 (MQ=255)
                                                                                              acCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCt                                                      <  1:303270/148‑1 (MQ=255)
                                                                                                cATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATg                                                   >  4:499618/1‑149 (MQ=255)
                                                                                                   gAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTaaaa                                                                         <  3:584759/124‑1 (MQ=255)
                                                                                                   gAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTaaaa                                                                         >  4:584759/1‑124 (MQ=255)
                                                                                                   gAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGaaa                                                <  3:512352/149‑1 (MQ=255)
                                                                                                    aaaGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGaaaa                                               <  3:659132/149‑1 (MQ=255)
                                                                                                     aaGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGGTATGaaaa                                               <  2:614866/148‑1 (MQ=255)
                                                                                                     aaGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGaaaa                                               <  1:738162/148‑1 (MQ=255)
                                                                                                     aaGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAAt                                              >  3:247101/1‑149 (MQ=255)
                                                                                                       gCCGCTCTGGGGCTTAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATaa                                            <  3:842977/149‑1 (MQ=255)
                                                                                                       gCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATaa                                            <  4:840048/149‑1 (MQ=255)
                                                                                                        ccGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACgg                                                                                               >  3:324508/1‑97 (MQ=255)
                                                                                                        ccGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACgg                                                                                               <  4:324508/97‑1 (MQ=255)
                                                                                                        ccGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATaa                                            >  1:152736/1‑148 (MQ=255)
                                                                                                         cGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACa                                          <  4:490597/149‑1 (MQ=255)
                                                                                                              tGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTAc                                      <  1:51629/148‑1 (MQ=255)
                                                                                                              tGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTACt                                     <  4:828839/149‑1 (MQ=255)
                                                                                                              tGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTACt                                     <  3:901532/149‑1 (MQ=255)
                                                                                                               ggggCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTACt                                     <  2:150348/148‑1 (MQ=255)
                                                                                                                    tGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACCTTACTGGTTa                                <  1:677521/148‑1 (MQ=255)
                                                                                                                    tGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTACTGGTTa                                >  1:228880/1‑148 (MQ=255)
                                                                                                                    tGAAATAAAAACGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTACTGTTAGCAACCGGACTTAATGGCAGATCACGGGCGCATACGCTCATGATTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTACTGGTTa                                >  2:66206/1‑148 (MQ=255)
                                                                                                                         tAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTACTGGTTACCTTGc                          >  4:725781/1‑149 (MQ=255)
                                                                                                                          aaaaCCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTACTGGTTACCTTGc                          <  2:814938/148‑1 (MQ=255)
                                                                                                                            aaCCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTACTGGTTACCTTGCtt                        >  1:379404/1‑148 (MQ=255)
                                                                                                                               cgcgCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTACt                                     <  2:577858/132‑1 (MQ=255)
                                                                                                                               cgcgCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTACt                                     >  1:577858/1‑132 (MQ=255)
                                                                                                                                    ccGGCAGCGAAAATTCACTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTACTGGTTACCTTGCTTTTCGGTCTg               >  4:566629/1‑149 (MQ=255)
                                                                                                                                         aGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTACTGGTTACCTTGCTTTTCGGTCTGGtttt          <  4:1284/149‑1 (MQ=255)
                                                                                                                                          gTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTACTGGTTACCTTGCTTTTCGGTCTGGttttt         <  3:106015/149‑1 (MQ=255)
                                                                                                                                          gTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTACTGGTTACCTTGCTTTTCGGTCTGGttttt         >  4:180970/1‑149 (MQ=255)
                                                                                                                                              aTTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTACTGGTTACCTTGCTTTTCGGTCTGGTTTTTTTaa     <  4:247101/149‑1 (MQ=255)
                                                                                                                                              aTTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTACTGGTTACCTTGCTTTTCGGTCTGGTTTTTTTa      <  1:355599/148‑1 (MQ=255)
                                                                                                                                               ttttCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTACTGGTTACCTTGCTTTTCGGTCTGGTTTTTTTaa     <  2:379404/148‑1 (MQ=255)
                                                                                                                                                 ttCGCTGCCGGGTGGTTTTTTTGCTGTTAACAAACAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTACTGGTTACCTTGCTTTTCGGTCTGGTTTTTTTAacca  <  3:180970/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACAAGAACATACCGGCAGTCAGTTGCGTATTGCGGCGTATGGCCCGCATGCCGCCAATGTTGTTGGACTGACCGACCAGACCGATCTCTTCTACACCATGAAAGCCGCTCTGGGGCTGAAATAAAACCGCGCCCGGCAGTGAATTTTCGCTGCCGGGTGGTTTTTTTGCTGTTAGCAACCAGACTTAATGGCAGATCACGGGCGCATACGCTCATGGTTAAAACATGAAGAGGGATGGTGCTATGAAAATAACATTACTGGTTACCTTGCTTTTCGGTCTGGTTTTTTTAACCA  >  NC_000913/403039‑403332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: